羊种布氏菌043强毒株BRA0434基因缺失突变株的构建与毒力的初步评价

目的检测BRA0434基因对羊种布氏菌043毒力的影响。方法利用同源重组的原理,以BRA0434基因外侧的同源臂序列构建重组质粒,将其电转化至布氏菌043感受态细胞,通过卡那抗性筛选和检测引物鉴定而获得羊种布氏菌043-ΔBRA0434缺失突变株,并以之和羊种布氏菌043以106 CFU剂量腹腔接种小鼠,又以100∶1的感染复数侵染小鼠巨噬细胞。结果与羊种布氏菌043相比,羊种布氏菌043-ΔBRA0434缺失突变株的载菌量和小鼠脾脏重量的影响都有一定程度的下降;侵染小鼠巨噬细胞试验结果显示羊种布氏菌043-ΔBRA0434缺失突变株的毒力较羊种布氏菌043有所降低。结论BRA0434基因的缺失会减轻羊种布氏菌043的毒力。

The expression and clinical significance of BRAF in prostate cancer

Objective:The aim of this study was to investigate the expression of BRAF of prostate cancer,and to explore its relations with clinic-pathological factors.Methods:Immunohistochemistry was used to detect the expression of BRAF in 74 cases of prostate cancer,and 51 cases of benign prostate hyperplasia(BPH).Results:The positive rate of BRAF had significant difference between patients with prostate cancer and BPH(P < 0.05).The expression of BRAF was correlated with grade and stage of prostate cancer(P < 0.05),but not to age of onset(P > 0.05).Conclusion:The expression of BRAF may play a role in the tumorigenesis and progression of colorectal cancer.BRAF could be a factor to diagnose the typing of prostate cancers and predict the prognosis of prostate cancers.

除草剂“Basta”对油菜植株和愈伤组织的生长分化的影响

通过研究除草剂“Basta”对3个油菜品种的植株和愈伤组织的生长及分化的影响,发现“Basta”对油菜植株和愈伤组织的生长及分化具有强烈的抑制作用,而且品种不同,抑制程度不同。并在此基础上找到合适的“Basta”筛选压,为以bra基因作为选择标记基因的基因转化试验莫定了基础。