Zebrafish (Brachydanio rerio) were exposed to PCBs (50 μg/L) for 4 weeks. The gill and the liver morphology were examined by transmission eletron microslogy. Results indicated that the membrane and partial cytoplasm ...Zebrafish (Brachydanio rerio) were exposed to PCBs (50 μg/L) for 4 weeks. The gill and the liver morphology were examined by transmission eletron microslogy. Results indicated that the membrane and partial cytoplasm of the epithelium cell in secondary lamella collapsed with lots of mucous and cell debris lying around. Hepatic cells which exposed to PCBs for 4 weeks showed the deforming nuclear deviated from cell centre, mitochondrim collapsed and rough-surfaced endoplasmic reticulum fractured. Furthermore, there were lots of lipofuscin granule and lipid droplet in the cytoplasm of the damaged cells.展开更多
为探究重金属和农药对斑马鱼的毒性与安全评价,选用硫酸铜、氰戊菊酯对斑马鱼进行急性毒性试验,以24～96 h半致死浓度(LC_(50))判定斑马鱼对这两种药物的敏感性。结果表明,硫酸铜24 hLC_(50)48h LC_(50)、72 h LC_(50)、96 h LC_(50)分...为探究重金属和农药对斑马鱼的毒性与安全评价,选用硫酸铜、氰戊菊酯对斑马鱼进行急性毒性试验,以24～96 h半致死浓度(LC_(50))判定斑马鱼对这两种药物的敏感性。结果表明,硫酸铜24 hLC_(50)48h LC_(50)、72 h LC_(50)、96 h LC_(50)分别为12 mg/L、7.9 mg/L、6.8 mg/L、5.4 mg/L;氰戊菊酯24 h LC_(50)、48 h LC_(50)、72 h LC_(50)、96h LC_(50)分别为1.2× 10^(-4)mg/L、0.9 × 10^(-4)mg/L、0 9×10^(-4)mg/L、0.9×10^(-4)/mg/L;硫酸铜安全质量浓度为1.03 mg/L,氰戊菊酯为1.52×10^(-5)mg/L。参照我国化学物质对鱼类毒性分级标准,判定硫酸铜对斑马鱼急性毒性为Ⅱ级,氰戊菊酯对斑马鱼急性毒性为Ⅰ级。展开更多
文摘Zebrafish (Brachydanio rerio) were exposed to PCBs (50 μg/L) for 4 weeks. The gill and the liver morphology were examined by transmission eletron microslogy. Results indicated that the membrane and partial cytoplasm of the epithelium cell in secondary lamella collapsed with lots of mucous and cell debris lying around. Hepatic cells which exposed to PCBs for 4 weeks showed the deforming nuclear deviated from cell centre, mitochondrim collapsed and rough-surfaced endoplasmic reticulum fractured. Furthermore, there were lots of lipofuscin granule and lipid droplet in the cytoplasm of the damaged cells.
文摘为探究重金属和农药对斑马鱼的毒性与安全评价,选用硫酸铜、氰戊菊酯对斑马鱼进行急性毒性试验,以24～96 h半致死浓度(LC_(50))判定斑马鱼对这两种药物的敏感性。结果表明,硫酸铜24 hLC_(50)48h LC_(50)、72 h LC_(50)、96 h LC_(50)分别为12 mg/L、7.9 mg/L、6.8 mg/L、5.4 mg/L;氰戊菊酯24 h LC_(50)、48 h LC_(50)、72 h LC_(50)、96h LC_(50)分别为1.2× 10^(-4)mg/L、0.9 × 10^(-4)mg/L、0 9×10^(-4)mg/L、0.9×10^(-4)/mg/L;硫酸铜安全质量浓度为1.03 mg/L,氰戊菊酯为1.52×10^(-5)mg/L。参照我国化学物质对鱼类毒性分级标准,判定硫酸铜对斑马鱼急性毒性为Ⅱ级,氰戊菊酯对斑马鱼急性毒性为Ⅰ级。
