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急性脑出血模型大鼠脑组织中差异表达基因测序的验证及功能分析 被引量:1
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作者 高玉广 钟洁 +3 位作者 黄德庆 马玉娟 廖煜雄 刘琦琦 《中国组织工程研究》 CAS 北大核心 2024年第20期3182-3189,共8页
背景:急性脑出血中存在差异表达的基因,这些基因与脑出血发生发展有关。目的:筛选急性脑出血大鼠模型脑组织中差异表达的基因和关键基因,并采用qPCR进行验证,分析关键基因与脑出血后神经功能、脑组织含水量的关系。方法:78只SD大鼠随机... 背景:急性脑出血中存在差异表达的基因,这些基因与脑出血发生发展有关。目的:筛选急性脑出血大鼠模型脑组织中差异表达的基因和关键基因,并采用qPCR进行验证,分析关键基因与脑出血后神经功能、脑组织含水量的关系。方法:78只SD大鼠随机分为2组:脑出血组大鼠于右侧尾状核部位注射胶原酶构建脑出血模型,假手术组大鼠于相同部位注射等量生理盐水。运用TRIzol法将2组大鼠的脑组织提取出RNA,转录组测序,筛选急性脑出血脑组织的差异表达的基因,经过qPCR验证,分析基因与脑出血后神经功能、脑组织含水量的关系,并结合生物信息学对关键基因进行GO、KEGG功能富集分析。结果与结论:①筛选出10个关键基因,分别是CXCL8、SERPINE1、TFPI2、CXCR4、GDA、KCNQ5、ERICH3、SCN3B、CACNA1E、CCL20;②脑出血组的关键基因GDA、KCNQ5、ERICH3、SCN3B、CACNA1E含量低于假手术组(P<0.05),CXCL8、SERPINE1、TFPI2、CXCR4、CCL20含量高于假手术组(P<0.05);③关键基因GDA、KCNQ5、ERICH3、SCN3B、CACNA1E含量与脑组织含水量、神经功能缺损评分正相关(P<0.05),CXCL8、SERPINE1、TFPI2、CXCR4、CCL20含量与脑组织含水量、神经功能缺损评分负相关(P<0.05);④GO分析显示基因在生物过程差异表达基因主要富集于白细胞趋化性、趋化因子介导的信号通路;细胞组成差异表达基因主要富集于阳离子通道复合物、离子通道复合物;分子功能差异表达基因主要富集于门控通道活动、离子通道活动;⑤KEGG分析示基因集中于TNF信号通路、谷氨酸能突触、GABA能突触;⑥结论:在脑出血中出现差异表达的基因为CXCL8、SERPINE1、TFPI2、CXCR4、GDA、KCNQ5、ERICH3、SCN3B、CACNA1E、CCL20,这些基因与脑出血后脑组织含水量、神经功能相关,这些基因主要富集在细胞组分、结合功能、细胞突起等相关的生物学功能上。 展开更多
关键词 脑出血 基因 差异性表达 神经功能 脑组织含水量 生物信息学分析
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二甲双胍对小鼠脑缺血再灌注损伤后认知功能的影响及其机制
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作者 彭志锋 马国英 +1 位作者 杨靖辉 刘颖 《山西医科大学学报》 CAS 2024年第5期589-593,共5页
目的探讨二甲双胍对脑缺血再灌注(I/R)小鼠认知功能的影响及其机制。方法采用随机数字表法将40只雄性C57BL/6小鼠[(23±2)g,7~8周龄]分为4组:sham组、I/R组、I/R+100 mg/kg二甲双胍组(低剂量组)和I/R+200 mg/kg二甲双胍组(高剂量组)... 目的探讨二甲双胍对脑缺血再灌注(I/R)小鼠认知功能的影响及其机制。方法采用随机数字表法将40只雄性C57BL/6小鼠[(23±2)g,7~8周龄]分为4组:sham组、I/R组、I/R+100 mg/kg二甲双胍组(低剂量组)和I/R+200 mg/kg二甲双胍组(高剂量组),每组10只。脑缺血组小鼠采用大脑中动脉阻断(MCAO)60 min后再灌注7 d,sham组除不插入细丝阻断外,其余手术步骤相同;低剂量组和高剂量组小鼠在脑缺血再灌注后4 h,每天分别用100,200 mg/kg二甲双胍溶液灌胃,持续7 d。采用Morris水迷宫实验评价小鼠学习和记忆相关指标;采用干湿质量法评估小鼠脑水肿情况;采用HE染色评估小鼠神经元形态变化;采用ELISA试剂盒检测小鼠脑组织脑源性神经营养因子(BDNF)含量和超氧化物歧化酶(SOD)活性。结果与sham组相比,I/R组小鼠自发交替正确率降低,逃避潜伏期增加,目标象限时间降低,穿越平台次数降低(均P<0.05);脑组织含水量增加(P<0.05);BDNF含量和SOD活性均降低(P<0.05);脑组织中变性神经元数量增多。与I/R组相比,低剂量组小鼠目标象限时间增加(P<0.05);高剂量组小鼠自发交替正确率增加(P<0.05),逃避潜伏期降低(P<0.05),目标象限时间增加(P<0.05),穿越平台次数增加(P<0.05),脑组织含水量降低(P<0.05),BDNF含量和SOD活性增加(P<0.05),神经元变性减少。结论200 mg/kg二甲双胍后处理可改善脑缺血再灌注小鼠认知功能,可能与脑水肿减轻、神经元变性改善、脑内BDNF含量和SOD活性增加有关。 展开更多
关键词 二甲双胍 缺血再灌注 水迷宫 脑源性神经营养因子 超氧化物歧化酶 小鼠
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睡眠剥夺大鼠脑肠肽改变在大鼠咽喉反流中的作用
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作者 张雪 郝梅 张延平 《听力学及言语疾病杂志》 CAS CSCD 北大核心 2024年第6期532-535,共4页
目的研究睡眠剥夺大鼠血清中脑肠肽改变在大鼠咽喉反流发病中的作用。方法选取40只健康成年雄性SD大鼠,分为实验组和对照组,对照组正常睡眠,实验组分为三组,每组10只,用改良多平台水环境法剥夺8 h的白天睡眠,分别持续1、2、3个月,用ELIS... 目的研究睡眠剥夺大鼠血清中脑肠肽改变在大鼠咽喉反流发病中的作用。方法选取40只健康成年雄性SD大鼠,分为实验组和对照组,对照组正常睡眠,实验组分为三组,每组10只,用改良多平台水环境法剥夺8 h的白天睡眠,分别持续1、2、3个月,用ELISA法检测各组外周血中胃泌素(MTL)和生长抑素(SS)浓度,放射免疫法检测外周血中胃动素(GAS)浓度,比较各组结果。结果睡眠剥夺2月组GAS浓度明显低于对照组,睡眠剥夺3月组GAS浓度明显高于1月组和2月组(P<0.05)。三个实验组与对照组之间比较MTL浓度差异均无统计学意义(P>0.05),睡眠剥夺2月组MTL浓度明显高于3月组(P<0.01)。各实验组与对照组之间、三个实验组之间SS浓度均无统计学差异。结论睡眠剥夺可能通过影响GAS和MTL的分泌与大鼠咽喉反流的发生相关。 展开更多
关键词 改良多平台水环境法 睡眠剥夺 脑肠肽 咽喉反流
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Protective effects of dl-3n-butylphthalide against diffuse brain injury 被引量:7
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作者 Yaning Zhao Jianmin Li +2 位作者 Pan Zhang Changxiang Chen Shuxing Li 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第28期2615-2624,共10页
DI-3n-butyiphthalide can effectively treat cerebral ischemia; however, the mechanisms underlying the effects of dl-3n-butylphthalide on microcirculation disorders following diffuse brain injury remain unclear. In this... DI-3n-butyiphthalide can effectively treat cerebral ischemia; however, the mechanisms underlying the effects of dl-3n-butylphthalide on microcirculation disorders following diffuse brain injury remain unclear. In this study, models of diffuse brain injury were established in Sprague-Dawley rats with the vertical impact method. DI-3n-butylphthalide at 80 and 160 mg/kg was given via intraperitoneal injection immediately after diffuse brain injury. Ultrastructural changes in the cerebral cortex were observed using electron microscopy. Cerebral blood flow was measured by laser Doppler flowmetry, vascular density was marked by tannic acid-ferric chloride staining, vascular permeability was es- timated by the Evans blue method, brain water content was measured using the dry-wet method, and rat behavior was measured by motor function and sensory function tests. At 6, 24, 48, and 72 hours after administration of dl-3n-butylphthalide, reduced cerebral ultrastructure damage, in- creased vascular density and cerebral blood flow, and improved motor and sensory functions were observed. Our findings demonstrate that dl-3n-butylphthalide may have protective effects against diffuse brain injury by ameliorating microcirculation disorder and reducing blood-brain barrier dam- age and cerebral edema. 展开更多
关键词 neural regeneration brain injury diffuse brain injury blood-brain barrier brain edema vasculardensity cerebral blood flow vascular permeability brain water content grants-supported paper NEUROREGENERATION
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Target inhibition of caspase-8 alleviates brain damage after subarachnoid hemorrhage 被引量:3
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作者 Da-Qiang Ke Zhi-Yang Chen +2 位作者 Zhou-Ling Li Xia Huang Hui Liang 《Neural Regeneration Research》 SCIE CAS CSCD 2020年第7期1283-1289,共7页
Caspase-8 plays an important role in the mediation of inflammation and the effect of its role in subarachnoid hemorrhage remains elusive.The nucleotide-binding oligomerization domain-like receptor protein 3 inflammaso... Caspase-8 plays an important role in the mediation of inflammation and the effect of its role in subarachnoid hemorrhage remains elusive.The nucleotide-binding oligomerization domain-like receptor protein 3 inflammasome has been postulated to mediate inflammation during SAH.The aim of the present study was to investigate the effects of caspase-8 inhibition on SAH injury and further elucidate the molecular mechanisms.In this study,a subarachnoid hemorrhage model was established by endovascular perforation process in adult male Sprague-Dawley rats.Z-IETD-FMK(0.5,1,2 mg/kg;an inhibitor of caspase-8)was delivered via intravenous(tail vein)injection immediately after subarachnoid hemorrhage.After 12 hours of subarachnoid hemorrhage,western blot assay showed that the expression of cleaved caspase-8 was significantly increased at 12 hours,peaked at 24 hours,and then decreased at 72 hours after subarachnoid hemorrhage.Immunofluorescence staining demonstrated that caspase-8 was expressed in microglia after subarachnoid hemorrhage.Z-IETDFMK significantly improved neurological deficits and reduced brain water content 24 hours after subarachnoid hemorrhage.The Morris water maze and rotarod test confirmed that Z-IETD-FMK significantly improved spatial learning and memory abilities and motor coordination at 21–27 days after subarachnoid hemorrhage.Furthermore,inhibition of caspase-8 activation reduced the expression of pyrin domain-containing 3,caspase-1,and interleukin-1βafter subarachnoid hemorrhage.In conclusion,our findings suggest that caspase-8 inhibition alleviates subarachnoid hemorrhage-induced brain injuries by suppressing inflammation.The study was approved by the Institutional Animal Ethics Committee of the First Affiliated Hospital,School of Medicine,Zhejiang University,China(approval No.2016-193)on February 25,2016. 展开更多
关键词 brain water content CASPASE-8 INFLAMMATION Morris water maze neurological function NEUROPROTECTION pyrin domain-containing 3 rotarod test subarachnoid hemorrhage Z-IETD-FMK
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Effect of thermal therapy using hot water bottles on brain natriuretic peptide in chronic hemodialysis patients
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作者 Yoko Uchiyama-Tanaka 《Health》 2013年第2期253-258,共6页
Introduction: The use of repeated thermal therapy for improving the symptoms of chronic heart failure (CHF) has been recently demonstrated. Usually, thermal therapy requires an infrared dry sauna. However, it is diffi... Introduction: The use of repeated thermal therapy for improving the symptoms of chronic heart failure (CHF) has been recently demonstrated. Usually, thermal therapy requires an infrared dry sauna. However, it is difficult for small clinics to acquire such an expensive and extensive system. The author assessed the efficacy of its substitution with hot water bottles. Moreover, there are no prior studies demonstrating the efficacy of thermal therapy in hemodialysis patients with chronic heart failure. Methods: The author evaluated plasma brain natriuretic peptide (BNP) levels in 98 hemodialysis patients in a clinic. A total of nine patients whose BNP levels were more than 500 pg/mL agreed to be enrolled in this study and received thermal therapy using hot water bottles. Results: Plasma BNP levels, a potential marker for CHF, tended to decrease (891 ± 448 pg/mL to 680 ± 339 pg/mL), but the difference was not significant (P = 0.0845). The oral temperature changed from 36.44℃± 0.45℃ to 37.04℃ ± 0.48℃ (+0.597℃, P < 0.0001). No side effects were experienced during the therapy. Moreover, most patients had an improvement in their symptoms and the ability to perform activities of daily living. Conclusion: Thermal therapy using hot water bottles is very safe and tends to reduce plasma BNP levels in hemodialysis patients with CHF. 展开更多
关键词 BNP brain NATRIURETIC Peptide CHRONIC Heart Failure CHRONIC KIDNEY Disease HEMODIALYSIS Hot water BOTTLE Thermal Therapy
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骨窗封闭对中重型颅脑创伤小鼠模型的影响
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作者 赵明宇 杨晨 +3 位作者 刘宇恒 李景 于明圣 王增光 《中国现代神经疾病杂志》 CAS 北大核心 2024年第6期425-434,共10页
目的探讨骨窗封闭对中重型颅脑创伤小鼠模型的影响。方法采用控制性皮质撞击法分别构建中型和重型颅脑创伤小鼠模型,随机分为中型颅脑创伤骨窗封闭组(中型骨窗封闭组,50只)、中型颅脑创伤骨窗未封闭组(中型骨窗未封闭组,50只)、重型颅... 目的探讨骨窗封闭对中重型颅脑创伤小鼠模型的影响。方法采用控制性皮质撞击法分别构建中型和重型颅脑创伤小鼠模型,随机分为中型颅脑创伤骨窗封闭组(中型骨窗封闭组,50只)、中型颅脑创伤骨窗未封闭组(中型骨窗未封闭组,50只)、重型颅脑创伤骨窗封闭组(重型骨窗封闭组,50只)、重型颅脑创伤骨窗未封闭组(重型骨窗未封闭组,50只),监测颅内压,测定脑组织含水量和脑水肿体积,采用改良神经功能缺损评分(mNSS)评估神经功能缺损程度,Morris水迷宫实验评估空间学习能力和记忆力,Nissl染色评估大脑皮质和海马CA1区神经元损伤程度。结果颅内压监测,无论中型还是重型颅脑创伤模型小鼠骨窗封闭组与骨窗未封闭组颅内压差异均有统计学意义(P=0.007,0.000),模型制备后不同观察时间点颅内压差异亦有统计学意义(P=0.000,0.000),其中,中型骨窗封闭组模型制备后第1天颅内压高于中型骨窗未封闭组(P=0.009),重型骨窗封闭组第1天(P=0.000)和第3天(P=0.038)颅内压高于重型骨窗未封闭组;模型制备后第7天中型骨窗封闭组(P=0.000,0.000)和重型骨窗封闭组(P=0.000,0.008)颅内压均低于第1天和第3天,第3天亦低于第1天(P=0.000,0.000),仅第7天中型骨窗未封闭组颅内压低于第1天(P=0.031)。脑组织含水量测定显示,重型骨窗封闭组模型制备后第1天(P=0.028)、第3天(P=0.023)和第7天(P=0.023)脑组织含水量均低于重型骨窗未封闭组。脑水肿体积测定,无论中型还是重型颅脑创伤模型小鼠骨窗封闭组脑水肿体积均小于骨窗未封闭组(P=0.021,0.037)。神经功能缺损程度评估,无论中型还是重型颅脑创伤模型小鼠骨窗封闭组与骨窗未封闭组模型制备后不同观察时间点mNSS评分差异均具有统计学意义(P=0.000,0.001),其中,模型制备后第7天中型骨窗封闭组(P=0.002)、中型骨窗未封闭组(P=0.013)、重型骨窗封闭组(P=0.009)mNSS评分均低于第1天,重型骨窗封闭组(P=0.006)和重型骨窗未封闭组(P=0.002)mNSS评分低于第3天。Morris水迷宫实验,重型骨窗封闭组小鼠平台潜伏期长于(P=0.045)、目标象限停留时间短于(P=0.025)重型骨窗未封闭组。Nissl染色显示,对于中型颅脑创伤模型小鼠,骨窗封闭组大脑皮质神经元Nissl小体密度减少,染色变浅;海马CA1区神经元Nissl小体密度减少,染色变浅,形态模糊。对于重型颅脑创伤模型小鼠,骨窗封闭组大脑皮质神经元Nissl小体染色变浅,染色模糊,可见较多异染颗粒;海马CA1区神经元胞体水肿,Nissl小体染色模糊。结论中型颅脑创伤模型小鼠,骨窗封闭虽在急性期增高颅内压,但对脑水肿程度、神经功能和认知功能无明显影响;重型颅脑创伤模型小鼠,骨窗封闭可导致颅内压升高、空间学习能力和记忆力减退,但可减轻脑水肿程度,应根据研究目的选择是否进行骨窗封闭。 展开更多
关键词 脑损伤 创伤性 减压颅骨切除术 颅内压 脑水肿 Morris水迷宫试验 虎斑小体 疾病模型 动物
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加味真武汤联合西药治疗腹膜透析合并慢性心力衰竭临床研究
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作者 彭玲 孙致健 +3 位作者 刘兴正 杨雪瑞 莫锦莹 宋高峰 《新中医》 CAS 2024年第16期1-5,共5页
目的:观察加味真武汤联合西药治疗腹膜透析(PD)合并慢性心力衰竭(CHF)阳虚水泛证的临床疗效。方法:选取54例PD合并CHF阳虚水泛证患者,采用随机数字表法分为对照组与观察组各27例。研究过程中对照组剔除2例、观察组剔除3例,最终纳入研究... 目的:观察加味真武汤联合西药治疗腹膜透析(PD)合并慢性心力衰竭(CHF)阳虚水泛证的临床疗效。方法:选取54例PD合并CHF阳虚水泛证患者,采用随机数字表法分为对照组与观察组各27例。研究过程中对照组剔除2例、观察组剔除3例,最终纳入研究对照组25例、观察组24例。对照组给予常规西药治疗,观察组在对照组基础上给予加味真武汤治疗,2组均治疗24周。比较2组临床疗效、中医证候积分及心功能指标[脑钠肽(BNP)、半乳糖凝集素-3 (Galectin-3)]水平。结果:治疗24周后,观察组总有效率79.17%,高于对照组28.00%,差异有统计学意义(P<0.05)。观察组中医证候积分较治疗前降低,且低于对照组,差异均有统计学意义(P<0.05);对照组中医证候积分与治疗前比较,差异无统计学意义(P>0.05)。观察组血清BNP、Galectin-3水平均较治疗前降低,且均低于对照组,差异均有统计学意义(P<0.05);对照组血清BNP、Galectin-3水平与治疗前比较,差异均无统计学意义(P>0.05)。结论:加味真武汤联合西药治疗PD合并CHF阳虚水泛证临床疗效较好,可有效改善临床症状,降低血清BNP、Galectin-3水平。 展开更多
关键词 慢性心力衰竭 腹膜透析 阳虚水泛证 加味真武汤 半乳糖凝集素-3 脑钠肽
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基于CT灌注自动化定量净摄水率预测急性缺血性脑卒中神经功能预后
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作者 滕晓萍 张久楼 +3 位作者 王悦 张墀 鲁珊珊 施海彬 《中国医学影像技术》 CSCD 北大核心 2024年第10期1466-1470,共5页
目的观察基于CT灌注(CTP)自动化定量净摄水率(CTP-aNWU)预测急性缺血性脑卒中(AIS)神经功能预后的价值。方法回顾性纳入145例急性前循环大血管闭塞性卒中患者,记录入院时及随访临床资料,分析Alberta卒中项目早期CT评分-净摄水率(ASPECTS... 目的观察基于CT灌注(CTP)自动化定量净摄水率(CTP-aNWU)预测急性缺血性脑卒中(AIS)神经功能预后的价值。方法回顾性纳入145例急性前循环大血管闭塞性卒中患者,记录入院时及随访临床资料,分析Alberta卒中项目早期CT评分-净摄水率(ASPECTS-NWU),基于平扫CT(NCCT)及CTP获得梗死核心CTP-aNWU;根据发病90天mRS评分结果将患者分为预后良好组(mRS≤2分,n=54)及预后不良组(mRS>2分,n=91),比较组间临床及影像学资料,筛选AIS神经功能预后的独立预测因素并评估其预测效能。结果组间年龄、入院NIHSS评分、入院ASPECTS,病灶ASPECTS-NWU、CTP-aNWU、梗死核心体积及低灌注因子差异均有统计学意义(P均<0.05);其中,梗死核心体积[OR=0.977(0.963~0.992),P=0.002]及CTP-aNWU[OR=0.876(0.793~0.969),P=0.010]均为AIS神经功能预后的独立预测因素。以单一CTP-aNWU预测AIS发病90天神经功能预后的受试者工作特征曲线下面积(AUC)为0.634[95%CI(0.550,0.713)],联合梗死核心体积为0.790[95%CI(0.714,0.853)],后者高于前者(Z=3.500,P<0.001)。结论CTP-aNWU为AIS神经功能预后的独立预测因素;联合梗死核心体积有助于提升预测效能。 展开更多
关键词 缺血性脑卒中 脑水肿 体层摄影术 X线计算机 预后 净摄水率
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Enhanced Expression of Aquaporin-9 in Rat Brain Edema Induced by Bacterial Lipopolysaccharides 被引量:2
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作者 王怀立 金润铭 +1 位作者 田培超 禚志红 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2009年第2期150-155,共6页
To investigate the role of AQP9 in brain edema, the expression of AQP9 in an infectious rat brain edema model induced by the injection of lipopolysaccharide (LPS) was examined. Immuno- histochemistry and reverse tra... To investigate the role of AQP9 in brain edema, the expression of AQP9 in an infectious rat brain edema model induced by the injection of lipopolysaccharide (LPS) was examined. Immuno- histochemistry and reverse transcription-polymerase chain reaction (RT-PCR) analysis demonstrated that the expressions of AQP9 mRNA and protein at all observed intervals were significantly increased in LPS-treated animals in comparison with the control animals. Time-course analysis showed that the first signs of blood-brain barrier disruption and the increase of brain water content in LPS-treated animals were evident 6 h after LPS injection, with maximum value appearing at 12 h, which coincided with the expression profiles of AQP9 mRNA and protein in LPS-treated animals. The further correlation analysis revealed strong positive correlations among the brain water content, the disruption of the blood-brain barrier and the enhanced expressions of AQP9 mRNA and protein in LPS-treated animals. These results suggested that the regulation of AQP9 expression may play im- portant roles in water movement and in brain metabolic homeostasis associated with the pathophysi- ology of brain edema induced by LPS injection. 展开更多
关键词 AQUAPORINS AQUAPORIN-9 LIPOPOLYSACCHARIDES infectious brain edema water channels
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Endogenous adult neurogenesis and cognitive function recovery following traumatic brain injury in the rat hippocampus 被引量:2
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作者 Wangmiao Zhao Linchun Huan +6 位作者 Yan Zhao Jie Zhao Qi Zhang Lin Zhang Rong Yan Shuyuan Yang Xinyu Yang 《Neural Regeneration Research》 SCIE CAS CSCD 2010年第9期645-650,共6页
BACKGROUND:Endogenous neural progenitor cells play a beneficial role for cognitive recovery following traumatic brain injury.However,there are few classification-control studies aimed at varying graded brain trauma.O... BACKGROUND:Endogenous neural progenitor cells play a beneficial role for cognitive recovery following traumatic brain injury.However,there are few classification-control studies aimed at varying graded brain trauma.OBJECTIVE:To observe the effects of adult endogenous neurogenesis on cognitive function repair and regeneration of neural progenitor cells following varying graded traumatic hippocampal injury to determine the significance of endogenous neurogenesis in the repair of brain injury.DESIGN,TIME AND SETTING:A randomized,controlled,animal experiment was performed at the Key Laboratory of Injuries,Variations and Regeneration of Nervous System,Tianjin Medical University General Hospital,from February to October 2009.MATERIALS:Mouse anti-rat 5-bromodeoxyuridine (BrdU) and neuronal nuclei (NeuN) monoclonal antibodies were purchased from Millipore Corporation,USA.METHODS:A total of 45 Wistar rats were randomly assigned to three groups.Mild and severe injury groups were respectively subjected to (182 ± 2) kPa and (284 ± 4) kPa lateral fluid percussion to establish models of brain injury,and the control group was subjected to surgery with no lateral fluid percussion.MAIN OUTCOME MEASURES:Cognitive function was estimated using the Morris water maze.Proliferation,survival,and differentiation of newly generated cells in the injured hippocampus were observed through the use of immunofluorescent staining.RESULTS:At 7 days post-injury,the number of BrdU+ cells in the hippocampal dentate gyrus significantly increased in the mild and severe injury groups compared with the control group (P〈0.01).At 61 days post-injury,the number of BrdU7NeuN+ cells in the hippocampal dentate gyrus was significantly greater in the mild injury group compared with the severe injury and control groups (P〈 0.01).In addition,the control group exhibited the greatest proportion of surviving cells that differentiated into mature neurons compared with the injury groups (P〈 0.01).Moreover,at 61 days post-injury,cognitive function in rats with mild injury recovered to normal levels,whereas the severe injury group exhibited cognitive deficits (P〈 0.01).CONCLUSION:Traumatic brain injury may be a stimulation factor for proliferation of neural progenitor cells in the adult hippocampus but severe brain trauma does not lead to an increased number of newly generated cells.Endogenous adult neurogenesis repairs neurological functions to an extent.However,recovery of neurological function remains limited following severe traumatic brain injury. 展开更多
关键词 traumatic brain injury HIPPOCAMPUS Morris water maze NEUROGENESIS cognitive function brain injury neural regeneration
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人神经干细胞移植对缺氧缺血性脑病大鼠神经修复的研究
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作者 丁亚兵 赵媛 +3 位作者 张凡 王倩 汪兆艳 栾佐 《华中科技大学学报(医学版)》 CAS CSCD 北大核心 2024年第3期287-294,共8页
目的探究移植人神经干细胞(hNSCs)治疗新生大鼠中、重度缺氧缺血性脑病(HIE)及后遗症期对神经的保护机制。方法取80只7日龄雄性SD大鼠,随机取其中55只采用Rice-Vannucci法制备HIE模型,建模后24 h对存活模型鼠采用Longa评分筛选出中、重... 目的探究移植人神经干细胞(hNSCs)治疗新生大鼠中、重度缺氧缺血性脑病(HIE)及后遗症期对神经的保护机制。方法取80只7日龄雄性SD大鼠,随机取其中55只采用Rice-Vannucci法制备HIE模型,建模后24 h对存活模型鼠采用Longa评分筛选出中、重度神经损伤大鼠并随机分为HIE+PBS组(PBS组,n=23)、HIE+hNSCs组(NSC组,n=23),从剩余25只大鼠中随机取23只作为假手术组(Sham组,n=23),Sham组只游离右侧颈总动脉,不予离断,也不予缺氧处理。三组同步给予药物抗排斥反应,PBS组与NSC组在建模后3 d分别经右侧脑室注射5μL PBS溶液或hNSCs悬液。移植后10 d,三组各随机取15只大鼠采用酶联免疫吸附实验(ELISA)检测脑内血管内皮生长因子(VEGF)、脑源性神经营养因子(BDNF)含量,采用免疫荧光染色观察两种因子在脑内的分布情况。移植后12周,通过免疫荧光染色观察NSC组大鼠脑内hNSCs的迁移、分化,以水迷宫实验对三组各剩余的8只大鼠进行神经功能检测。结果移植后10 d,NSC组VEGF、BDNF两种因子含量均最多,PBS组次之,Sham组最少,NSC组两种因子含量均显著高于Sham组(均P<0.05)。移植后12周,hNSCs向HIE大鼠两侧脑半球迁移,以右侧为主,成熟神经元分化率约30%。移植后12周,水迷宫实验中,PBS组潜伏期均长于Sham组及NSC组(均P<0.05),PBS组穿越平台次数均少于Sham及NSC组(均P<0.05);而Sham组与NSC组结果比较,差异无统计学意义(均P>0.05)。结论脑室移植hNSCs通过加强HIE大鼠脑内VEGF、BDNF的旁分泌效应,及迁移并分化为神经元的替代作用,从而促进HIE大鼠后遗症期神经损伤的修复,为HIE治疗提供新思路。 展开更多
关键词 新生儿缺氧缺血性脑病 脑源性神经营养因子 细胞迁移 细胞分化 水迷宫
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MK-801 attenuates lesion expansion following acute brain injury in rats: a meta-analysis 被引量:1
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作者 Nan-Xing Yi Long-Yun Zhou +8 位作者 Xiao-Yun Wang Yong-Jia Song Hai-Hui Han Tian-Song Zhang Yong-Jun Wang Qi Shi Hao Xu Qian-Qian Liang Ting Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2019年第11期1919-1931,共13页
OBJECTIVE: To evaluate the efficacy and safety of MK-801 and its effect on lesion volume in rat models of acute brain injury.DATA SOURCES: Key terms were "stroke","brain diseases","brain injur... OBJECTIVE: To evaluate the efficacy and safety of MK-801 and its effect on lesion volume in rat models of acute brain injury.DATA SOURCES: Key terms were "stroke","brain diseases","brain injuries","brain hemorrhage, traumatic","acute brain injury","dizocilpine maleate","dizocilpine","MK-801","MK801","rat","rats","rattus" and "murine". PubMed, Cochrane library, EMBASE, the China National Knowledge Infrastructure, WanFang database, the VIP Journal Integration Platform(VJIP) and SinoMed databases were searched from their inception dates to March 2018.DATA SELECTION: Studies were selected if they reported the effects of MK-801 in experimental acute brain injury. Two investigators independently conducted literature screening, data extraction, and methodological quality assessments.OUTCOME MEASURES: The primary outcomes included lesion volume and brain edema. The secondary outcomes included behavioral assessments with the Bederson neurological grading system and the water maze test 24 hours after brain injury.RESULTS: A total of 52 studies with 2530 samples were included in the systematic review. Seventeen of these studies had a high methodological quality. Overall, the lesion volume(34 studies, n = 966, MD =-58.31, 95% CI:-66.55 to-50.07;P < 0.00001) and degree of cerebral edema(5 studies, n = 75, MD =-1.21, 95% CI:-1.50 to-0.91;P < 0.00001) were significantly decreased in the MK-801 group compared with the control group. MK-801 improved spatial cognition assessed with the water maze test(2 studies, n = 60, MD =-10.88, 95% CI:-20.75 to-1.00;P = 0.03) and neurological function 24 hours after brain injury(11 studies, n = 335, MD =-1.04, 95% CI:-1.47 to-0.60;P < 0.00001). Subgroup analysis suggested an association of reduction in lesion volume with various injury models(34 studies, n = 966, MD =-58.31, 95% CI:-66.55 to-50.07;P = 0.004). Further network analysis showed that 0–1 mg/kg MK-801 may be the optimal dose for treatment in the middle cerebral artery occlusion animal model.CONCLUSION: MK-801 effectively reduces brain lesion volume and the degree of cerebral edema in rat models of experimental acute brain injury, providing a good neuroprotective effect. Additionally, MK-801 has a good safety profile, and its mechanism of action is well known. Thus, MK-801 may be suitable for future clinical trials and applications. 展开更多
关键词 nerve REGENERATION acute brain injury neurological function spatial cognition water MAZE test LESION volume brain EDEMA rat systematic review META-ANALYSIS neural REGENERATION
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Effects of exogenous ganglioside-1 on learning and memory in a neonatal rat model of hypoxia-ischemia brain injury
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作者 Shizhi Li Nong Xiao +5 位作者 Xiaoping Zhang Ling Liu Liyun Lin Siyuan Chen Yuxia Chen Bei Xu 《Neural Regeneration Research》 SCIE CAS CSCD 2008年第9期1004-1009,共6页
BACKGROUND: Exogenous ganglioside-1 (GM1) can cross the blood-brain barrier and play a protective role against hypoxia-ischemia-induced brain damage. OBJECTIVE: To examine the possible mechanisms of exogenous GM1 ... BACKGROUND: Exogenous ganglioside-1 (GM1) can cross the blood-brain barrier and play a protective role against hypoxia-ischemia-induced brain damage. OBJECTIVE: To examine the possible mechanisms of exogenous GM1 protection in hypoxia-ischemia-induced brain damage in a neonatal rat model by measuring changes in brain mass, pathological morphology, growth-associated protein-43 expression, and neurobehavioral manifestations. DESIGN, TIME AND SETTING: A randomized block-design study was performed at the Immunohistochemistry Laboratory of the Pediatric Research Institute, Children's Hospital of Chongqing Medical University from August 2005 to August 2006. MATERIALS: A total of 36 neonatal, 7-day-old, Sprague Dawley rats were used in this experiment. The hypoxia-ischemia-induced brain damage model was established by permanently occluding the right carotid artery, followed by oxygen inhalation at a low concentration (8% O2, 92% N2) for 2 hours, METHODS: All rats were randomly divided into the following groups: GMI, model, and sham operation, with 12 rats each group. Rats in the GM 1 and model groups received hypoxic/ischemic-induced brain damage. Rats in the GM1 group received injections of GM1 (i.p., 20 mg/kg) at 0, 24, 48, 72, 96, 120, and 144 hours following models established, and rats in the model group were administered (i.p.) the same amount of saline. The right carotid artery was separated, but not ligated, in the sham operation group rats. MAIN OUTCOME MEASURES: At 1 week after surgery, expression of growth-associated protein-43, a marker of neural development and plasticity, was detected in the hippocampal CA3 region by immunohistochemistry. Brain mass was measured, and the pathological morphology was observed. At 4 weeks after surgery, behavioral changes in the remaining rats were tested by Morris water maze, and growth-associated protein-43 expression was measured. RESULTS: (1) In the GMI and sham operation groups, growth-associated protein-43 expression was greater in the hippocampal CA3 region compared to the model group 1 week after surgery (P 〈 0.05). In all three groups, brain weight of the right hemisphere was significantly less than the left hemisphere, in particular in the model group (P 〈 0.05). In the GMI group, the weight difference between two hemispheres, as well as the extent of damage in the right hemisphere, was less than the model group (P 〈 0.01 ). In the sham operation Uoup, brain tissue consisted of integrated structures and ordered cells. In the model group, the cerebral cortex layers of the right hemisphere were not defined, neurons were damaged, and neurons were disarranged in the hippocampal area. In the GM1 group, neurons were dense in the right cerebral cortex and hippocampal area, with no significant change in glial proliferation. (2) The average time of escape latency in the GM1 group was shortened 4 weeks alter surgery, and significantly less than the model group (P 〈 0.05). In addition, the frequency platform passing in the GMI group was significantly greater than the model group (P 〈 0.01). CONCLUSION: Exogenous GM1 may reduce brain injury and improve learning and memory in hypoxia-ischemia-induced brain damage rats. This protection may be associated with increased growth-associated protein-43 expression, which is involved in neuronal remodeling processes. 展开更多
关键词 GANGLIOSIDE growth-associated protein-43 hypoxia-ischemia brain damage Morris water maze
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Effects of a Free Water Protocol on Inpatients in a Neuro-Rehabilitation Setting 被引量:1
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作者 Sepideh Pooyania Laura Vandurme +1 位作者 Reesa Daun Caitlin Buchel 《Open Journal of Therapy and Rehabilitation》 2015年第4期132-138,共7页
Background: Dysphagia is common among stroke and acquired brain injury (ABI) patients and may result in aspiration. To reduce the risk of thin liquid aspiration, patients are often restricted to thickened fluids. Ther... Background: Dysphagia is common among stroke and acquired brain injury (ABI) patients and may result in aspiration. To reduce the risk of thin liquid aspiration, patients are often restricted to thickened fluids. There is considerable clinical interest in the risks and benefits of offering oral water intake, in the form of water protocols, to patients with thin-liquid dysphagia. Objectives: A controlled pilot study of a free water protocol was undertaken at Riverview Health Centre, in Winnipeg, Manitoba to assess benefits, feasibility and possible complications of free water protocol. Methods: The study examined 16 individuals with stroke or ABI who were randomly assigned to either a control group on thickened fluids (six subjects) or a treatment group (ten subjects) that followed a free water protocol. The average length of time in the study was 4.3 weeks with a range of 1 - 17 weeks among all participants. Results: Throughout the study, there was no incidence of pneumonia in any of the sixteen participants. No statistically significant difference was noted in the weekly total liquid intake between the treatment group and the control group. Perceived swallowing related care results suggest statistically significant improvements from initial to final measures for both the treatment group (p = 0.004) and the control group (p = 0.02). However, a comparison of the change in both measures, between the treatment and control groups, shows no statistically significant differences. Conclusion: This pilot study suggests the need for larger scale work in order to more accurately identify the effects of free water protocols. 展开更多
关键词 Free water PROTOCOL DYSPHAGIA Stroke and ACQUIRED brain Injury
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针灸与康复疗法干预脑缺血模型大鼠神经功能及肠道菌群的变化 被引量:8
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作者 崔振华 林夏妃 +3 位作者 陈永敏 林烨 李关羽 宋振华 《中国组织工程研究》 CAS 北大核心 2023年第17期2692-2698,共7页
背景:针灸与康复训练均能够有效缓解脑缺血患者症状,目前有关两者联合治疗是否对脑缺血患者神经功能障、肠道菌群失调具有调控作用尚不明确。目的:探究针灸联合康复疗法对脑缺血大鼠神经功能、肠道菌群的影响。方法:将60只SD大鼠按随机... 背景:针灸与康复训练均能够有效缓解脑缺血患者症状,目前有关两者联合治疗是否对脑缺血患者神经功能障、肠道菌群失调具有调控作用尚不明确。目的:探究针灸联合康复疗法对脑缺血大鼠神经功能、肠道菌群的影响。方法:将60只SD大鼠按随机数表法随机分为假手术组、模型组、针刺组、康复组和针康组(n=12)。除假手术组外,其余各组建立脑缺血大鼠模型;假手术组仅分离左侧颈总动脉;针刺组用头穴丛刺干预,康复组进行任务导向性跑台训练,针康组同时给予头穴丛针刺和任务导向性跑台训练干预,干预14 d。观察造模后4 h及1,7,14 d各组大鼠神经功能情况,检测干预14 d后大鼠脑含水量变化,Western blot检测缺血半暗带神经元生长相关蛋白(生长相关蛋白43、神经丝蛋白200、排斥指导分子a)表达,试剂盒检测脑组织乳酸脱氢酶、超氧化物歧化酶、丙二醛水平,酶联免疫吸附法检测脑组织肿瘤坏死因子α、白细胞介素1β质量浓度,实时荧光定量PCR检测大鼠粪便中大肠杆菌、双歧杆菌、乳酸杆菌和肠球菌变化。结果与结论:①造模后4 h及1,7,14 d,假手术组无神经功能障碍,模型组大鼠出现明显神经功能障碍(P<0.05);②与同时段模型组相比,针刺组和康复组大鼠神经功能缺损随着干预时间延长有所改善(P<0.05),而针康组大鼠神经功能改善更显著(P<0.01);③与模型组比较,针刺组、康复组大鼠缺血半暗带生长相关蛋白43、神经丝蛋白200表达上调(P<0.05),排斥指导分子a表达下调(P<0.05);大鼠脑组织含水量和脑组织中乳酸脱氢酶、丙二醛、肿瘤坏死因子α、白细胞介素1β质量浓度降低(P<0.05),超氧化物歧化酶活性升高(P<0.05);粪便中大肠杆菌、肠球菌数量明显增多(P<0.05),双歧杆菌、乳酸菌数量明显降低(P<0.05);且针康组上述指标改善更为显著(P<0.01);④提示针灸联合康复疗法可通过刺激缺血半暗带区神经元再生改善脑缺血大鼠神经功能,减轻氧化应激反应和炎症反应,调节肠道菌群紊乱,对缺血性脑卒中具有保护作用。 展开更多
关键词 针康法 针刺 脑缺血 神经功能 脑含水量 炎症 肠道菌群 组织工程
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填精补髓中药汤剂对脑出血模型小鼠Keap1-Nrf2-ARE信号通路的影响
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作者 朱沛维 时克 《中国药业》 CAS 2023年第S02期7-10,共4页
目的探讨填精补髓中药汤剂对脑出血模型小鼠Keap1-Nrf2-ARE信号通路的影响。方法将48只Wistar雄性小鼠分为假手术组、模型组(均予等体积生理盐水灌胃),阳性药组(每100 g体质量灌胃脑血康30 mg)、中药汤剂组(每100 g体质量灌胃填精补髓中... 目的探讨填精补髓中药汤剂对脑出血模型小鼠Keap1-Nrf2-ARE信号通路的影响。方法将48只Wistar雄性小鼠分为假手术组、模型组(均予等体积生理盐水灌胃),阳性药组(每100 g体质量灌胃脑血康30 mg)、中药汤剂组(每100 g体质量灌胃填精补髓中药2 mL),各12只。采用Rynkowski法建立脑出血小鼠模型,各组均于麻醉清醒后给药,每天1次,比较各组小鼠的神经功能(Longa)评分、脑含水量、血肿周围脑组织病理变化情况。应用Western blot法检测Nrf2信号通路相关蛋白(Nrf2,Keap1,HO-1)的表达水平,采用实时荧光定量聚合酶链式反应(RT-PCR)法检测Nrf2,Keap1,HO-1 mRNA的表达水平。结果术后1,3 d,模型组小鼠的Longa评分、脑含水量均较假手术组高,阳性药组、中药汤剂组小鼠的Longa评分、脑含水量均低于模型组,差异均有统计学意义(P<0.05);模型组小鼠的血肿区被大量红细胞浸润,伴随神经细胞数量减少,存在明显的间质水肿,神经细胞排列稀疏,中药汤剂组、阳性药组小鼠的红细胞浸润程度、间质水肿程度均轻于模型组;中药汤剂组、阳性药组小鼠术后3 d的脑组织Nrf2,Keap1,HO-1 mRNA及蛋白表达水平均显著低于模型组(P<0.05)。结论填精补髓中药汤剂可通过激活Keap1-Nrf2-ARE信号通路而发挥抗氧化作用,改善脑出血模型小鼠的神经功能障碍及脑水肿,预防脑出血后继发损伤。 展开更多
关键词 填精补髓中药汤剂 脑出血小鼠模型 Keap1-Nrf2-ARE信号通路 脑含水量
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母体妊娠期精神压力暴露对子代鼠脑白质超微机构及其认知功能的影响
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作者 程艳 郭建伟 +1 位作者 朱斌 伊敏敏 《海南医学院学报》 2023年第16期1207-1216,共10页
目的:探究母体妊娠期精神压力暴露对子代鼠认知功能及脑白质超微结构的影响。方法:亲代鼠分为压力束缚暴露组(PE组)和对照组(C组)两组,雌性SD大鼠阴道涂片阳性日期为妊娠第0天。PE组亲代雌鼠妊娠第14~20天暴露于束缚压力(3次/天),持续45... 目的:探究母体妊娠期精神压力暴露对子代鼠认知功能及脑白质超微结构的影响。方法:亲代鼠分为压力束缚暴露组(PE组)和对照组(C组)两组,雌性SD大鼠阴道涂片阳性日期为妊娠第0天。PE组亲代雌鼠妊娠第14~20天暴露于束缚压力(3次/天),持续45 min~1 h/次。1月龄子代鼠行为学测试(Morris水迷宫和Y迷宫),后心脏灌注固定脑标本并置入混合固定液中。使用现代体视学方法计算脑白质总体积、有髓神经纤维总长度及总体积和髓鞘总长度及总体积,对髓鞘内外直径及内外周长进行计分析。结果:(1)行为学测试:与C组相比,PE组小鼠第3、4天Morris水迷宫定位航行试验潜伏期显著延长、空间探索试验平台所在象限滞留时间百分比和穿越平台有效区域次数显著减少,Y迷宫新颖臂探索距离、探索次数及探索时间明显增加(P<0.05)。(2)与C组相比,PE组小鼠脑白质总体积、有髓神经纤维总长度、有髓神经纤维总体积、髓鞘总体积明显下降(P<0.05),髓鞘内直径、外直径明显下降(P<0.05),内周长、外周长及内外周长差明显增加(P<0.05)。(3)行为学测试结果与脑白质超微结构测定结果间存在相关关系。结论:母体妊娠期精神压力暴露可损害子代鼠认知功能及脑白质及其超微结构,认知功能降低与脑白质损害间存在相关关系。 展开更多
关键词 精神压力暴露 认知功能 脑白质 MORRIS水迷宫 Y迷宫
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石甘散联合丙戊酸钠对癫痫模型幼鼠脑损伤保护作用和认知功能的影响
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作者 李丽丽 《齐齐哈尔医学院学报》 2023年第3期214-218,共5页
目的探讨石甘散联合丙戊酸钠对癫痫模型幼鼠脑损伤保护作用及对其认知功能的影响。方法采用随机数表法将60只健康Wistar幼鼠分为对照组、模型组、丙戊酸钠组、石甘散组和联合组共五组,每组各12只。给予对照组幼鼠连续28天腹腔注射1 ml 0... 目的探讨石甘散联合丙戊酸钠对癫痫模型幼鼠脑损伤保护作用及对其认知功能的影响。方法采用随机数表法将60只健康Wistar幼鼠分为对照组、模型组、丙戊酸钠组、石甘散组和联合组共五组,每组各12只。给予对照组幼鼠连续28天腹腔注射1 ml 0.9%氯化钠注射液,其余四组幼鼠连续28天腹腔注射1 ml 2 mg/ml戊四氮溶液。建模成功后,给予对照组和模型组幼鼠每日2次0.9%氯化钠注射液灌胃,给予丙戊酸钠组幼鼠每日1次200 mg/kg丙戊酸钠灌胃,给予石甘散组幼鼠每日1次1 g/kg石甘散溶液灌胃,给予联合组幼鼠每日1次200 mg/kg丙戊酸钠灌胃和1次1 g/kg石甘散溶液灌胃,所有幼鼠均连续灌胃治疗28天。治疗结束后进行Morris水迷宫测试,随后处死幼鼠并提取海马组织血液,分别采用TUNEL法和JC-1法检测海马组织神经元凋亡情况和神经元线粒体跨膜电位(Δψm),采用酶联免疫吸附法检测血清神经元特异性烯醇化酶(NSE)。结果(1)对照组幼鼠1-5天的逃避潜伏期显著短于其余四组(P<0.01);丙戊酸钠组、石甘散组和联合组幼鼠1-5天的逃避潜伏期均显著短于模型组;联合组幼鼠1-5天的逃避潜伏期显著短于丙戊酸钠组和石甘散组(P<0.01)。(2)五组幼鼠平台穿越次数比较有显著差异,对照组幼鼠穿越次数最多,其次为联合组,模型组幼鼠穿越次数最少(P<0.01)。(3)五组幼鼠海马组织神经元细胞凋亡比例比较有显著差异,对照组比例最低,其次为联合组,模型组最高(P<0.01)。(4)五组幼鼠海马组织神经元Δψm比较有显著差异,对照组Δψm最高,其次为联合组,模型组最低(P<0.01)。(5)五组幼鼠血清NSE水平比较有显著差异,对照组NSE水平最低,其次为联合组,模型组最高(P<0.01)。(6)丙戊酸钠组和石甘散组幼鼠1-5天的逃避潜伏期、平台穿越次数、海马组织神经元细胞凋亡比例、海马组织神经元Δψm以及血清NSE比较无显著差异(P>0.05)。结论石甘散联合丙戊酸钠能够有效改善癫痫幼鼠认知功能,阻断线粒体异常电位保护线粒体功能,抑制海马组织神经元凋亡,减轻脑损伤程度。 展开更多
关键词 癫痫 石甘散 丙戊酸钠 脑损伤 认知功能 MORRIS水迷宫 神经元凋亡 线粒体跨膜电位 NSE
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脑出血大鼠脑组织MDA、TNF-α含量变化及其依达拉奉干预效应的研究 被引量:19
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作者 崔洁 曹参祥 +1 位作者 郑静 王景周 《第三军医大学学报》 CAS CSCD 北大核心 2007年第11期1032-1035,共4页
目的观察3-甲基-1-苯基-2-吡唑啉-5-酮(MCI-186,商品名为依达拉奉)对出血性脑组织的保护作用和可能机制。方法将96只Sprague-Dawley大鼠按随机数字表法分为假手术组、脑出血模型组、依达拉奉治疗组,每组32只。采用自体血脑内注入法制作... 目的观察3-甲基-1-苯基-2-吡唑啉-5-酮(MCI-186,商品名为依达拉奉)对出血性脑组织的保护作用和可能机制。方法将96只Sprague-Dawley大鼠按随机数字表法分为假手术组、脑出血模型组、依达拉奉治疗组,每组32只。采用自体血脑内注入法制作大鼠脑出血模型,假手术组只进针不注血。依达拉奉治疗组大鼠腹腔注射依达拉奉(3mg/kg),分别于造模前30min及造模后1次/12h。于6、24、72、168h分别用Longa标准测定神经功能缺损;干、湿重法检测脑含水量;酶联免疫吸附法(ELISA)和硫代巴比妥酸法测定血肿周围组织中脑组织TNF-α和丙二醛(malondialde-hyde,MDA)的含量。结果与假手术组相比,脑出血模型组大鼠神经功能缺损评分、脑含水量、TNF-α和MDA含量均显著增高(P<0.05);与脑出血模型组相比,依达拉奉治疗组大鼠神经功能缺损评分、脑含水量、TNF-α和MDA含量显著下降(P<0.05)。结论依达拉奉对出血后脑组织具有保护作用,其机制可能与抑制MDA和TNF-α的活性有关。 展开更多
关键词 依达拉奉 脑出血 脑含水量 丙二醛 肿瘤坏死因子-Α
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