In this study,we isolated a polysaccharide from Branchiostoma belcheri (PBB) by enzymatic protein hydrolysis and alcohol precipitation.We investigated the effects of PBB supplementation on DNA oxidation and growth of ...In this study,we isolated a polysaccharide from Branchiostoma belcheri (PBB) by enzymatic protein hydrolysis and alcohol precipitation.We investigated the effects of PBB supplementation on DNA oxidation and growth of the transplanted tumor cells Sarcoma (S180) in mice.Sixty healthy Kunming mice weighing between 18 and 25 g were randomly assigned to 6 groups,each consisting of 10 animals.All the mice,except for the blank control group,were inoculated with S180 sarcoma cells into the axilla of the left foreleg.PBB was given to mice by gavage at doses of 0 (model control),25,50,or 100 mg/kg b.w.in 0.2 ml saline for 30 days.The fifth group of S180-mice was given cytoxan (50 mg/kg) by peritoneal injection as a positive control group.The animals had free access to food and water.The mice were sacrificed after the final treatment and blood was quickly collected.Spontaneous and oxidized DNA damage of peripheral lymphocytes induced by H2O2 were analyzed by SCGE.O6-methyl-guanine (O6-MeG) was measured by high-performance capillary zone electrophoresis.The average tumor weights (0.856-1.118 g) of the three PBB groups were significantly lower than that of the model control group (1.836 g) (p<0.05).The tumor inhibition ratios of the PBB groups were 39.1%-53.4% and similar to the cytoxan positive group (57.5%).There were no significant differences in spontaneous DNA damage in peripheral lymphocytes among the groups.The oxidative DNA damage induced by 10 μmol/L H2O2 in the 50 and 100 mg/kg b.w.groups were 246.1 AU and 221.7 AU,respectively,both of which were significantly lower than that in the model group (289.0 AU;p<0.05).The plasma concentrations of O6-MeG in the 25,50,and 100 mg/kg supplemented groups were 2.09 μmol/L,1.86 μmol/L,and 1.63 μmol/L,respectively,all of which were significantly lower than that of the model group (2.67 μmol/L;p<0.05).These results indicated that PBB may have antioxidative activity and thus reduce oxidation-induced DNA damage.展开更多
Amphioxus, also called lancelet or cephalochordate, is a promising model organism owning to its particularly evolu- tionary position, simple genome content and comparable body plan to that of vertebrates （Holland et ...Amphioxus, also called lancelet or cephalochordate, is a promising model organism owning to its particularly evolu- tionary position, simple genome content and comparable body plan to that of vertebrates （Holland et aL, 2004; Bertrand and Escriva, 2011）. However, use of amphioxus as a model or- ganism has been limited for many years because of lack of an efficient genomic modification method. Recently, several revolutionary gene targeting methods that could induce directed mutations, insertions and deletions at intended target sites, have been developed （Gaj et al., 2013）.展开更多
Amphioxus is the ancestor of vertebrates 5×108 a ago, it is a typical transitional sample of evolution from invertebrates to vertebrates. Inter simple sequence repeats (ISSRs) and random amplified polymorphic DNA...Amphioxus is the ancestor of vertebrates 5×108 a ago, it is a typical transitional sample of evolution from invertebrates to vertebrates. Inter simple sequence repeats (ISSRs) and random amplified polymorphic DNAs (RAPDs) technologies were applied to detect the genetic variation of 3 bulking samples and individually sampled populations in nowadays Xiamen sea areas (Xiekou, Nanxian and Huangcuo) where the amphioxuses are alive. For the bulking sampled populations, 5 ISSR and 10 RAPD primers generated 357 bands, of which 181 (50.7%) were polymorphic. Nei index and UPGMA statistical analysis indicated that amphioxuses in these 3 areas could be divided into 2 groups. The genetic distance between animals in Nanxian and Huangcuo areas was 0.07 and classified into 1 group, while the population in Xiekou belonged to another group because its genetic distances in Nanxian and Huangcuo were 0.12 and 0.14, respectively. The result was in accordance with the morphological comparison among animals from those areas. For individually sampled population, Shannon's index of genetic diversity was used to partition the diversity of the animals among these 3 sea areas, and the results showed that the indices in populations of Xiekou, Nanxian and Huangcuo, were 0.583, 0.482 and 0.374, respectively. The linear regression equation analysis for amphioxus' genetic diversity versus the environment factors revealed that granularity/sorting coefficient and water depth were the most important factors that affect amphioxus genetic diversity. On the basis of the results, the suggestions for amphioxus resource conservation in Xiamen sea areas are put forward.展开更多
文摘In this study,we isolated a polysaccharide from Branchiostoma belcheri (PBB) by enzymatic protein hydrolysis and alcohol precipitation.We investigated the effects of PBB supplementation on DNA oxidation and growth of the transplanted tumor cells Sarcoma (S180) in mice.Sixty healthy Kunming mice weighing between 18 and 25 g were randomly assigned to 6 groups,each consisting of 10 animals.All the mice,except for the blank control group,were inoculated with S180 sarcoma cells into the axilla of the left foreleg.PBB was given to mice by gavage at doses of 0 (model control),25,50,or 100 mg/kg b.w.in 0.2 ml saline for 30 days.The fifth group of S180-mice was given cytoxan (50 mg/kg) by peritoneal injection as a positive control group.The animals had free access to food and water.The mice were sacrificed after the final treatment and blood was quickly collected.Spontaneous and oxidized DNA damage of peripheral lymphocytes induced by H2O2 were analyzed by SCGE.O6-methyl-guanine (O6-MeG) was measured by high-performance capillary zone electrophoresis.The average tumor weights (0.856-1.118 g) of the three PBB groups were significantly lower than that of the model control group (1.836 g) (p<0.05).The tumor inhibition ratios of the PBB groups were 39.1%-53.4% and similar to the cytoxan positive group (57.5%).There were no significant differences in spontaneous DNA damage in peripheral lymphocytes among the groups.The oxidative DNA damage induced by 10 μmol/L H2O2 in the 50 and 100 mg/kg b.w.groups were 246.1 AU and 221.7 AU,respectively,both of which were significantly lower than that in the model group (289.0 AU;p<0.05).The plasma concentrations of O6-MeG in the 25,50,and 100 mg/kg supplemented groups were 2.09 μmol/L,1.86 μmol/L,and 1.63 μmol/L,respectively,all of which were significantly lower than that of the model group (2.67 μmol/L;p<0.05).These results indicated that PBB may have antioxidative activity and thus reduce oxidation-induced DNA damage.
基金supported by the National Natural Science Foundation of China (Nos.31071110,30830023 and 31101631)the Scientific and Technical Innovation Committee of Shenzhen,China (No.CXZZ20120614164555920)
文摘Amphioxus, also called lancelet or cephalochordate, is a promising model organism owning to its particularly evolu- tionary position, simple genome content and comparable body plan to that of vertebrates （Holland et aL, 2004; Bertrand and Escriva, 2011）. However, use of amphioxus as a model or- ganism has been limited for many years because of lack of an efficient genomic modification method. Recently, several revolutionary gene targeting methods that could induce directed mutations, insertions and deletions at intended target sites, have been developed （Gaj et al., 2013）.
基金supported by the Science and Technology ofFujian Province under contract No.2001J033.
文摘Amphioxus is the ancestor of vertebrates 5×108 a ago, it is a typical transitional sample of evolution from invertebrates to vertebrates. Inter simple sequence repeats (ISSRs) and random amplified polymorphic DNAs (RAPDs) technologies were applied to detect the genetic variation of 3 bulking samples and individually sampled populations in nowadays Xiamen sea areas (Xiekou, Nanxian and Huangcuo) where the amphioxuses are alive. For the bulking sampled populations, 5 ISSR and 10 RAPD primers generated 357 bands, of which 181 (50.7%) were polymorphic. Nei index and UPGMA statistical analysis indicated that amphioxuses in these 3 areas could be divided into 2 groups. The genetic distance between animals in Nanxian and Huangcuo areas was 0.07 and classified into 1 group, while the population in Xiekou belonged to another group because its genetic distances in Nanxian and Huangcuo were 0.12 and 0.14, respectively. The result was in accordance with the morphological comparison among animals from those areas. For individually sampled population, Shannon's index of genetic diversity was used to partition the diversity of the animals among these 3 sea areas, and the results showed that the indices in populations of Xiekou, Nanxian and Huangcuo, were 0.583, 0.482 and 0.374, respectively. The linear regression equation analysis for amphioxus' genetic diversity versus the environment factors revealed that granularity/sorting coefficient and water depth were the most important factors that affect amphioxus genetic diversity. On the basis of the results, the suggestions for amphioxus resource conservation in Xiamen sea areas are put forward.
