Objective:To determine the anti-breast cancer activities and the safety oral consumption of Dillenia suffruticosa root aqueous extract(DRAE)in BALB/c mice.Methods:In the anti-breast cancer study,female BALB/c mice wer...Objective:To determine the anti-breast cancer activities and the safety oral consumption of Dillenia suffruticosa root aqueous extract(DRAE)in BALB/c mice.Methods:In the anti-breast cancer study,female BALB/c mice were divided into five groups(n=12),which were(1)positive control(with breast cancer,untreated),(2)negative control(without breast cancer,untreated)and other three groups of mice with breast cancer treated with 1 000,500 and 250 mg/kg of DRAE,respectively,by oral gavage for 28 days.All mice except from the negative control group were injected into the mammary fat pad with 4T1 cells(1×1054T1 cells/0.1 m L of phosphate buffer solution).DRAE was administered orally on Day 11 after the tumor has developed.Results:The tumor volume of the 1 000 mg/kg of DRAE group reduced significantly compared to the positive control while treatment with 500 mg/kg of DRAE had significantly inhibited metastasis to the heart.In the acute toxicity study,treatment with up to5 000 mg/kg of DRAE was not toxic to the animals,indicating its safety when a large amount of this plant extract was ingested.Based on the sub-acute toxicity study,treatment of the highest dose of DRAE(1 000 mg/kg)had mild liver toxicity indicated by mild focal hemorrhage.Conclusions:DRAE possesses anti-breast cancer properties but at the same time it shows mild toxicity to the liver.The non observable adverse effect dose for DRAE is500 mg/kg.展开更多
In the present study, films and coatings for food applications based on a gum exudate from Brea tree （Cercidium praecox） were formulated and the influence of plasticizer content on their functional properties was ev...In the present study, films and coatings for food applications based on a gum exudate from Brea tree （Cercidium praecox） were formulated and the influence of plasticizer content on their functional properties was evaluated. Brea gum is a renewable resource available in semi-desert areas, extracted by native people. Films were produced by casting method and characterized through water vapour permeability, mechanical （tensile strength and elongation at break）, morphological and optical properties. Glycerol was used as plasticizer. Brea gum films presented good visual aspect, transparency and amber colour. Microscopic observation showed a dense and homogeneous structure. Film wettability increased with glycerol content due to hydrophilic nature of the plasticizer. Solubility of films in water increased with temperature. Water sorption isotherms of films at 25 ~C showed that at high aw （above 0.50）, the film matrix swells altering its structure and properties. Water vapour permeability remained practically constant up to 20% of glycerol content and then increases linearly with the plasticizer. The addition of glycerol decreased the opacity and tensile strength. The best film properties were obtained at 20% of glycerol concentration.展开更多
AIM:To assess the effectiveness of hepatitis B virus(HBV) vaccination program among fully vaccinated children.METHODS:A national community based crosssectional study was carried out in 6 governorates representing Egyp...AIM:To assess the effectiveness of hepatitis B virus(HBV) vaccination program among fully vaccinated children.METHODS:A national community based crosssectional study was carried out in 6 governorates representing Egypt. A total of 3600 children aged from 9 mo to 16 years who were fully vaccinated with HBV vaccine during infancy were recruited. Face to face interviews were carried out and sera were evaluated for hepatitis B surface antigen(HBsA g),anti-HBV core antibodies(total) and quantitative detection of hepatitis B surface antibody using enzyme linked immunoassays techniques. Samples positive to HBs Ag/anti-HBV core antibodies were subjected to quantitative HBV-DNA detection by real time polymerase chain reaction with 3.8 IU/L detection limit. RESULTS:Sero-protection was detected among 2059 children(57.2%) with geometric mean titers 75.4 ± 3.6 IU/L compared to 3.1 ± 2.1 IU/L among nonseroprotected children. Multivariate logistic analysis revealed that older age and female gender were the significant predicting variables for having non seroprotective level,with adjusted odds ratio 3.3,9.1and 14.2 among children aged 5 to < 10,10 to < 15 and ≥ 15 years respectively compared to those < 5 years and 1.1 among girls compared to boys with P < 0.01. HBs Ag was positive in 0.11% and breakthrough infection was 0.36% and 0.39% depending on positivity of anti-HBc and DNA detection respectively. The prevalence of HBV infection was significantly higher among children aged ≥ 7 years(0.59%) compared to 0.07% among younger children with odds ratio equal to 8.4(95%CI:1.1-64.2) and P < 0.01.The prevalence was higher among girls(0.48%) than boys(0.29%) with P > 0.05. C ON C LU S I ON :T he E gy pt ian c ompuls or y H B V vaccination program provides adequate protection. Occult HBV infection exists among apparently healthy vaccinated children. Adherence to infection control measures is mandatory.展开更多
目的探讨保乳手术和改良根治术治疗早期乳腺early-stage breast cancer,EBC)的临床疗效。方法方便选取该院自2013年6月—2015年6月间收治的TNM分期为Ⅰ期的EBC患者86例作为研究对象,依据手术指征和患者意愿分成两组,行保乳手术治疗的患...目的探讨保乳手术和改良根治术治疗早期乳腺early-stage breast cancer,EBC)的临床疗效。方法方便选取该院自2013年6月—2015年6月间收治的TNM分期为Ⅰ期的EBC患者86例作为研究对象,依据手术指征和患者意愿分成两组,行保乳手术治疗的患者46例,采用改良根治术患者40例,对比两组患者术后并发症发生率、使用乳腺癌生活质量量表(QLQ-BR23)评估两组患者手术治疗效果;随访2年,记录两组患者复发/转移率、死亡率情况。结果保乳手术组患者术后并发症发生率为4.35%,改良组为22.50%(χ~2=6.320,P<0.05);术后6个月保乳组患者QLQBR23评分优于改良组,组间数据对比差异有统计学意义(P<0.05);术后随访2年,保乳组患者复发率为4.35%、转移率为2.17%、死亡率为4.35%,改良组依次为0.00%、5.00%、5.00%,组间数据差异无统计学意义(P>0.05)。结论早期乳腺癌患者采用保乳手术治疗并发症发生率低、术后患者的生活质量表现优秀,术后近期复发率和死亡率与改良根治术无显著差异,因此值得推广使用。展开更多
基金Supported by the Universiti Putra Malaysia with Grant No.9366600
文摘Objective:To determine the anti-breast cancer activities and the safety oral consumption of Dillenia suffruticosa root aqueous extract(DRAE)in BALB/c mice.Methods:In the anti-breast cancer study,female BALB/c mice were divided into five groups(n=12),which were(1)positive control(with breast cancer,untreated),(2)negative control(without breast cancer,untreated)and other three groups of mice with breast cancer treated with 1 000,500 and 250 mg/kg of DRAE,respectively,by oral gavage for 28 days.All mice except from the negative control group were injected into the mammary fat pad with 4T1 cells(1×1054T1 cells/0.1 m L of phosphate buffer solution).DRAE was administered orally on Day 11 after the tumor has developed.Results:The tumor volume of the 1 000 mg/kg of DRAE group reduced significantly compared to the positive control while treatment with 500 mg/kg of DRAE had significantly inhibited metastasis to the heart.In the acute toxicity study,treatment with up to5 000 mg/kg of DRAE was not toxic to the animals,indicating its safety when a large amount of this plant extract was ingested.Based on the sub-acute toxicity study,treatment of the highest dose of DRAE(1 000 mg/kg)had mild liver toxicity indicated by mild focal hemorrhage.Conclusions:DRAE possesses anti-breast cancer properties but at the same time it shows mild toxicity to the liver.The non observable adverse effect dose for DRAE is500 mg/kg.
文摘In the present study, films and coatings for food applications based on a gum exudate from Brea tree （Cercidium praecox） were formulated and the influence of plasticizer content on their functional properties was evaluated. Brea gum is a renewable resource available in semi-desert areas, extracted by native people. Films were produced by casting method and characterized through water vapour permeability, mechanical （tensile strength and elongation at break）, morphological and optical properties. Glycerol was used as plasticizer. Brea gum films presented good visual aspect, transparency and amber colour. Microscopic observation showed a dense and homogeneous structure. Film wettability increased with glycerol content due to hydrophilic nature of the plasticizer. Solubility of films in water increased with temperature. Water sorption isotherms of films at 25 ~C showed that at high aw （above 0.50）, the film matrix swells altering its structure and properties. Water vapour permeability remained practically constant up to 20% of glycerol content and then increases linearly with the plasticizer. The addition of glycerol decreased the opacity and tensile strength. The best film properties were obtained at 20% of glycerol concentration.
基金The Science and Technology Development Fund(STDF),Egypt,No.1611
文摘AIM:To assess the effectiveness of hepatitis B virus(HBV) vaccination program among fully vaccinated children.METHODS:A national community based crosssectional study was carried out in 6 governorates representing Egypt. A total of 3600 children aged from 9 mo to 16 years who were fully vaccinated with HBV vaccine during infancy were recruited. Face to face interviews were carried out and sera were evaluated for hepatitis B surface antigen(HBsA g),anti-HBV core antibodies(total) and quantitative detection of hepatitis B surface antibody using enzyme linked immunoassays techniques. Samples positive to HBs Ag/anti-HBV core antibodies were subjected to quantitative HBV-DNA detection by real time polymerase chain reaction with 3.8 IU/L detection limit. RESULTS:Sero-protection was detected among 2059 children(57.2%) with geometric mean titers 75.4 ± 3.6 IU/L compared to 3.1 ± 2.1 IU/L among nonseroprotected children. Multivariate logistic analysis revealed that older age and female gender were the significant predicting variables for having non seroprotective level,with adjusted odds ratio 3.3,9.1and 14.2 among children aged 5 to < 10,10 to < 15 and ≥ 15 years respectively compared to those < 5 years and 1.1 among girls compared to boys with P < 0.01. HBs Ag was positive in 0.11% and breakthrough infection was 0.36% and 0.39% depending on positivity of anti-HBc and DNA detection respectively. The prevalence of HBV infection was significantly higher among children aged ≥ 7 years(0.59%) compared to 0.07% among younger children with odds ratio equal to 8.4(95%CI:1.1-64.2) and P < 0.01.The prevalence was higher among girls(0.48%) than boys(0.29%) with P > 0.05. C ON C LU S I ON :T he E gy pt ian c ompuls or y H B V vaccination program provides adequate protection. Occult HBV infection exists among apparently healthy vaccinated children. Adherence to infection control measures is mandatory.
文摘目的探讨保乳手术和改良根治术治疗早期乳腺early-stage breast cancer,EBC)的临床疗效。方法方便选取该院自2013年6月—2015年6月间收治的TNM分期为Ⅰ期的EBC患者86例作为研究对象,依据手术指征和患者意愿分成两组,行保乳手术治疗的患者46例,采用改良根治术患者40例,对比两组患者术后并发症发生率、使用乳腺癌生活质量量表(QLQ-BR23)评估两组患者手术治疗效果;随访2年,记录两组患者复发/转移率、死亡率情况。结果保乳手术组患者术后并发症发生率为4.35%,改良组为22.50%(χ~2=6.320,P<0.05);术后6个月保乳组患者QLQBR23评分优于改良组,组间数据对比差异有统计学意义(P<0.05);术后随访2年,保乳组患者复发率为4.35%、转移率为2.17%、死亡率为4.35%,改良组依次为0.00%、5.00%、5.00%,组间数据差异无统计学意义(P>0.05)。结论早期乳腺癌患者采用保乳手术治疗并发症发生率低、术后患者的生活质量表现优秀,术后近期复发率和死亡率与改良根治术无显著差异,因此值得推广使用。
