Gene targeting technology is an important means to investigate gene functions, but its efficiency of gene targeting is very low, especially for somatic cell targeting. Artificially induced double-strand breaks （DSB）...Gene targeting technology is an important means to investigate gene functions, but its efficiency of gene targeting is very low, especially for somatic cell targeting. Artificially induced double-strand breaks （DSB） and triplex forming oligonucleotide （TFO） are currently developed methods to improve the targeting efficiency. This paper summarized the basic principles, design ideas and application in gene targeting efficiency improvement of these two methods, analyzed and com- pared their characteristics, and finally proposed prospects for their future development.展开更多
The characteristics of the flow field associated with a multi-hole combined external rotary bit have been studied by means of numerical simulation in the framework of an RNG k-εturbulence model,and compared with the ...The characteristics of the flow field associated with a multi-hole combined external rotary bit have been studied by means of numerical simulation in the framework of an RNG k-εturbulence model,and compared with the results of dedicated rock breaking drilling experiments.The numerical results show that the nozzle velocity and dynamic pressure of the nozzle decrease with an increase in the jet distance,and the axial velocity of the nozzle decays regularly with an increase in the dimensionless jet distance.Moreover,the axial velocity related to the nozzle with inclination angle 20°and 30°can produce a higher hole depth,while the radial velocity of the nozzle with 60°inclination can enlarge the hole diameter.The outcomes of the CFD simulations are consistent with the actual dynamic rock breaking and pore forming process,which lends credence to the present results and indicates that they could be used as a reference for the future optimization of systems based on the multi-hole combined external rotary bit technology.展开更多
A new load surface based approach to the reliability analysis of caisson-type breakwater is proposed. Uncertainties of the horizontal and vertical wave loads acting on breakwater are considered by using the so-called ...A new load surface based approach to the reliability analysis of caisson-type breakwater is proposed. Uncertainties of the horizontal and vertical wave loads acting on breakwater are considered by using the so-called load surfaces, which can be estimated as functions of wave height, water level, and so on. Then, the first-order reliability method（FORM） can be applied to determine the probability of failure under the wave action. In this way, the reliability analysis of breakwaters with uncertainties both in wave height and in water level is possible. Moreover, the uncertainty in wave breaking can be taken into account by considering a random variable for wave height ratio which relates the significant wave height to the maximum wave height. The proposed approach is applied numerically to the reliability analysis of caisson breakwater under wave attack that may undergo partial or full wave breaking.展开更多
基金Supported by Shandong Swine Industry Technology System and Science and Technology Planning Program for Basic Research in Qingdao City(12-1-4-14-jch)
文摘Gene targeting technology is an important means to investigate gene functions, but its efficiency of gene targeting is very low, especially for somatic cell targeting. Artificially induced double-strand breaks （DSB） and triplex forming oligonucleotide （TFO） are currently developed methods to improve the targeting efficiency. This paper summarized the basic principles, design ideas and application in gene targeting efficiency improvement of these two methods, analyzed and com- pared their characteristics, and finally proposed prospects for their future development.
基金the Science and Technology Innovation and Entrepreneurship Fund of China Coal Technology Engineering Group(2019-TD-QN038,2019-TDQN017)Enterprise Independent Innovation Guidance Project(2018ZDXM05,2019YBXM30).
文摘The characteristics of the flow field associated with a multi-hole combined external rotary bit have been studied by means of numerical simulation in the framework of an RNG k-εturbulence model,and compared with the results of dedicated rock breaking drilling experiments.The numerical results show that the nozzle velocity and dynamic pressure of the nozzle decrease with an increase in the jet distance,and the axial velocity of the nozzle decays regularly with an increase in the dimensionless jet distance.Moreover,the axial velocity related to the nozzle with inclination angle 20°and 30°can produce a higher hole depth,while the radial velocity of the nozzle with 60°inclination can enlarge the hole diameter.The outcomes of the CFD simulations are consistent with the actual dynamic rock breaking and pore forming process,which lends credence to the present results and indicates that they could be used as a reference for the future optimization of systems based on the multi-hole combined external rotary bit technology.
基金supported by Basic Science Research Program through the National Research Foundation of Korea(NRF)funded by the Ministry of Education,Science and Technology(Grant No.NRF-2012R1A1A4A01010830)
文摘A new load surface based approach to the reliability analysis of caisson-type breakwater is proposed. Uncertainties of the horizontal and vertical wave loads acting on breakwater are considered by using the so-called load surfaces, which can be estimated as functions of wave height, water level, and so on. Then, the first-order reliability method（FORM） can be applied to determine the probability of failure under the wave action. In this way, the reliability analysis of breakwaters with uncertainties both in wave height and in water level is possible. Moreover, the uncertainty in wave breaking can be taken into account by considering a random variable for wave height ratio which relates the significant wave height to the maximum wave height. The proposed approach is applied numerically to the reliability analysis of caisson breakwater under wave attack that may undergo partial or full wave breaking.
