Silencing Filamin A Inhibits the Invasion and Migration of Breast Cancer Cells by Up-regulating 14-3-3σ

Filamin A and 14-3-3-σ are closely associated with the development of breast cancer. However, the exact relationship between them is still unknown. The present study aimed to examine the interaction of filamin A with 14-3-3-σ in the invasion and migration of breast cancer. RNA interference technology was employed to silence filamin A in MDA-MB-231 cells. Real-time PCR and Westem blotting were used to detect the expression of filamin A and 14-3-3-σ at mRNA and protein levels, respectively. Double immunofluorescence was applied to show their colocalization morphologically. Wound healing assay and Trans-well assay were used to testify the migration and invasion of MDA-MB-231 cells in filamin A-silenced cells. The results showed that silencing filamin A significantly increased the mRNA and protein levels of 14-3-3σ. In addition, double immunofluorescence displayed that filamin A and 14-3-3σ were predominantly colocalized in the cytoplasm of MDA-MB-231 cells. Silencing filamin A led to the enhanced fluorescence of 14-3-3σ. Furthermore, cell functional experiments showed that silencing filamin A inhibited the migration and invasion of MDA-MB-231 cells in vitro. In conclusion, silencing filamin A may inhibit the invasion and migration of breast cancer cells by upregulating 14-3-3σ.

Microstructural evolution of pre-twinned Mg alloy with annealing temperature and underlying boundary migration mechanism

This study investigates the variations in the microstructural characteristics of a pre-twinned Mg alloy with the temperature of the subsequent annealing treatment.To this end,a rolled AZ31 alloy is compressed to 3%plastic strain along the rolling direction(RD)to activate{10-12}twinning and is subsequently annealed at 200,250,300,350,and 400℃.Numerous{10-12}twins are formed throughout the compressed material,leading to the formation of a RD-oriented texture.At an annealing temperature of 200℃,no microstructural variations occur during annealing.As the annealing temperature increases from 250 to 400℃,the residual strain energy and remaining twin boundaries of the annealed material decrease owing to the promoted static recovery and the increased area fraction of twin-free grown grains.Consequently,an increase in the annealing temperature results in a gradual microstructural transition from a fully twinned grain structure to a completely twin-free grain structure.The microstructural evolution during annealing is predominantly governed by the movement of high-angle grain boundaries via a strain-induced boundary migration mechanism,and a few twin boundaries migrate above 350℃because of their lower boundary energy.The boundary migration behavior and resultant microstructural evolution are discussed in detail based on the variations in boundary mobility and driving force for boundary migration with annealing temperature.

Circular RNA AKT3 governs malignant behaviors of esophageal cancer cells by sponging miR-17-5p

BACKGROUND Recent studies have demonstrated that circular RNA AKT3(circAKT3)plays a crucial role in regulating the malignant phenotypes of tumor cells.However,the potential effects of circAKT3 on esophageal cancer have not been investigated.AIM To illuminate the role of circAKT3 in malignant behaviors of esophageal cancer cells and its underlying mechanism.METHODS Clinical samples were collected to detect the expression of circAKT3.The role of circAKT3 in proliferation,migration,invasion,and apoptosis of esophageal cancer cells was evaluated using Cell Counting Kit-8,wound healing assays,Transwell assays,and fluorescence analysis,respectively.The target of circAKT3 was screened and identified using an online database and luciferase reporter assay.A xenograft nude mouse model was established to investigate the role of circAKT3 in vivo.RESULTS In vitro assays showed that proliferative,migratory,and invasive capacities of esophageal cancer cells were significantly enhanced by circAKT3 overexpression.Furthermore,miR-17-5p was screened as the target of circAKT3,and miR-17-5p antagonized the effects of circAKT3 on esophageal cancer cells.Moreover,we identified RHOC and STAT3 as the direct target molecules of miR-17-5p,and circAKT3 facilitated expression of RHOC and STAT3 by inhibiting miR-17-5p.In vivo assays showed circAKT3 knockdown inhibited growth of esophageal cancer.CONCLUSION CircAKT3 contributed to the malignant behaviors of esophageal cancer in vitro and in vivo by sponging miR-17-5p thus providing a potential target for treatment of esophageal cancer.

lncRNACNN3-206 activates intestinal epithelial cell apoptosis and invasion by sponging miR-212, an implication for Crohn’s disease

BACKGROUND Statistics indicate that the incidence of Crohn’s disease(CD)is rising in many countries.The poor understanding on the pathological mechanism has limited the development of effective therapy against this disease.Previous studies showed that long noncoding RNAs(lncRNAs)could be involved in autoimmune diseases including CD,but the detailed molecular mechanisms remain unclear.AIM To identify the differentially expressed lncRNAs in the intestinal mucosa associated with CD,and to characterize their pathogenic role(s)and related mechanisms.METHODS The differential expression of lncRNAs was screened by high-throughput RNA sequencing,and the top candidate genes were validated in an expanded cohort by real-time PCR.The regulatory network was predicted by bioinformatic software and competitive endogenous RNA analysis,and was characterized in Caco-2 and HT-29 cell culture using methods of cell transfection,real-time PCR,Western blotting analysis,flow cytometry,and cell migration and invasion assays.Finally,these findings were confirmed in vivo using a CD animal model.RESULTS The 3'end of lncRNACNN3-206 and the 3’UTR of Caspase10 contain highaffinity miR212 binding sites.lncRNACNN3-206 expression was found to be significantly increased in intestinal lesions of CD patients.Activation of the lncRNACNN3-206-miR-212-Caspase10 regulatory network led to increased apoptosis,migration and invasion in intestinal epithelial cells.Knockdown of lncRNACNN3-206 expression alleviated intestinal mucosal inflammation and tissue damage in the CD mouse model.CONCLUSION lncRNACNN3-206 may play a key role in CD pathogenesis.lncRNACNN3-206 could be a therapeutic target for CD treatment.

Long noncoding RNA negative regulator of antiviral response contributes to pancreatic ductal adenocarcinoma progression via targeting miR-299-3p

BACKGROUND Pancreatic ductal cancer(PDAC)has high malignancy and poor prognosis.Long noncoding RNAs(lncRNAs)are associated with high levels of malignancy,including PDAC.However,the biological and clinical significance of negative regulator of antiviral response(NRAV)in PDAC is unclear.AIM To study the regulatory role of lncRNA NRAV in PDAC.METHODS GEPIA analyzed lncRNA NRAV and miRNA(miR-299-3p)expression levels in PDAC tissues and measured them in PDAC cells by quantitative measurements in real time.The specific role of NRAV and miR-299-3p in cell proliferation and transfer potential was evaluated by cell formation analysis,Cell Counting Kit-8 and Transwell analysis.The relationship between NRAV and miR-299-3p was studied by predictive bioinformatics,RNA immunoassay,and fluorescence enzyme analysis.In vivo experiments included transplantation of simulated tumor cells under naked mice.RESULTS The expression level of lncRNA NRAV was higher in both tumor tissues and cell lines of PDAC and was negatively associated with the clinical survival of PDAC patients.Functionally,overexpression of NRAV promoted cell proliferation and metastasis of PDAC cells,while knockdown of NRAV reversed these effects.Finally,NRAV was performed as a molecular sponge of miR-299-3p.Moreover,overexpression of miR-299-3p could reverse the promoting effects of NRAV on cell proliferation and metastasis of PDAC cells.CONCLUSION NRAV facilitates progression of PDAC as a molecular sponge of miR-299-3p and may be a potential molecular marker for diagnosis and treatment of PDAC.

β-咔啉类生物碱对SGC-7901细胞迁移、细胞侵袭和相关通路基因的影响

目的探索β-咔啉类生物碱对SGC-7901细胞迁移、细胞侵袭,和PI3K/AKT及MAPK/ERK通路基因mRNA,以及蛋白水平的抑制作用。方法通过CCK-8法测定不同浓度条件下β-咔啉类生物碱对胃癌SGC-7901细胞增殖的抑制率;通过Transwell小室测定β-咔啉类生物碱对SGC-7901细胞迁移、侵袭能力的影响;利用q RT-PCR、Western blotting技术检测相关基因mRNA及蛋白的表达差异。结果随着β-咔啉类生物碱浓度增加,人胃癌SGC-7901细胞增殖抑制率逐渐升高,IC50=13.364μg/m L;阳性对照组(5-FU,5μg/m L)、β-咔啉类生物碱组中Transwell小室内SGC-7901细胞均可见显著减少(P<0.01),且β-咔啉类生物碱组SGC-7901细胞数目较阳性对照组明显降低(P<0.01);阳性对照组、β-咔啉类生物碱实验组GRB2、PI3Kp85a、FAK基因mRNA表达水平较空白对照组显著降低(P<0.05),而STAT3、ERK1、ERK2基因mRNA表达水平无统计学差异(P>0.05);β-咔啉类生物碱组PI3Kp85a、p-ERK1/2、AKT蛋白表达水平显著低于空白对照组与阳性对照组(P<0.05)。结论较5-FU,β-咔啉类生物碱具有更有效的抑制胃癌SGC-7901细胞迁移与侵袭能力,而该机制可能与β-咔啉类生物碱抑制PI3Kp85a、p-ERK1/2、AKT蛋白表达水平有关。

Self-aggregating behavior of poly(4-vinyl pyridine)and the potential in mitigating sand production based onπ-πstacking interaction

Unconsolidated sandstone reservoirs are most susceptible to sand production that leads to a dramatic oil production decline.In this study,the poly(4-vinyl pyridine)(P_(4)VP)incorporated with self-aggregating behavior was proposed for sand migration control.The P_(4)VP could aggregate sand grains spontaneously throughπ-πstacking interactions to withstand the drag forces sufficiently.The influential factors on the self-aggregating behavior of the P_(4)VP were evaluated by adhesion force test.The adsorption as well as desorption behavior of P_(4)VP on sand grains was characterized by scanning electron microscopy and adhesion force test at different pH conditions.The result indicated that the pH altered the forms of surface silanol groups on sand grains,which in turn affected the adsorption process of P_(4)VP.The spontaneous dimerization of P_(4)VP molecules resulting from theπ-πstacking interaction was demonstrated by reduced density gradient analysis,which contributed to the self-aggregating behavior and the thermally reversible characteristic of the P_(4)VP.Dynamic sand stabilization test revealed that the P_(4)VP showed wide pH and temperature ranges of application.The production of sands can be mitigated effectively at 20-130℃ within the pH range of 4-8.

An Improved vTPM-VM Live Migration Protocol

To prevent malicious virtual machine from harming the security of vTPM-VM live migration process, we propose an im- proved vTPM-VM live migration protocol which uses a TPM-based integrity verification policy and a specific encryption scheme to enhance security. The TPM-based integrity verification policy is presented to ensure that all participating entities in this process are trustworthy. In data transfer phase, the specific encryp- tion scheme is designed to associate the decipher process with one certain platform status so that only the destination platform can gain the key data of the migrated VM and vTPM instance. The security of this new protocol is analyzed. The results show that this protocol can effectively resist most of the attacks in the proc- ess ofvTPM-VM live migration.

CircRNA_Maml2 promotes the proliferation and migration of intestinal epithelial cells after severe burns by regulating the miR-93-3p/FZD7/Wnt/β-catenin pathway

Background:Circular RNA(circRNA)plays key regulatory roles in the development of many diseases.However the biological functions and potential molecular mechanisms of circRNA in the injury and repair of intestinal mucosa in mice after severe burns are yet to be elucidated.Methods:Cell counting kit-8(CCK-8),5-ethynyl-2-deoxyuridine(EdU),wound healing and transwell assays were used to detect cell proliferation and migration ability.Real-time quantitative PCR was used to identify the expression of circRNA,microRNA and messenger RNA.Nuclear and cytoplas-mic separation experiments were employed to perceive the location of circRNA_Maml2.Finally,in vitro and in vivo experiments were conducted to study the repairing effect of circRNA_Maml2 on the intestinal mucosa of mice after severe burns.Results:When compared with the control group,the expression of circRNA_Maml2 was sig-nificantly reduced in the severe burn group.Furthermore,overexpression of circRNA_Maml2 promoted the proliferation and migration of CT26.wt cells in vivo and the repair of damaged intestinal mucosa in vitro.CircRNA_Maml2 acted as a sponge adsorption molecule for miR-93-3p to enhance the expression of frizzled class receptor 7 and activate the downstream Wnt/β-catenin pathway,thereby promoting the repair of the intestinal mucosa.Conclusions:Our findings demonstrate that circRNA_Maml2 regulates the miR-93-3p/FZD7/Wnt/β-catenin pathway and promotes the repair of damaged intestinal mucosa.Hence,circRNA_Maml2 is a potential therapeutic target to promote intestinal mucosal repair.

An Olefinic 1,2-α-Boryl Migration Enables 1,2-Bis(boronic esters)via Radical-Polar Crossover Reaction

A radical-induced 1,2-α-boryl migration through radical polar crossover reactions has been described.In this work,in situ formed vinyldiboron“ate”complexes from alkenyl Grignard reagent and diborylalkanes react with commercial radical precursors under light initiation.This three-component process enables diborylation of alkene.This protocol features high atom economy,a broad substrate scope as well as good functional group toleration with mild conditions.

Maternal miR-202-5p is required for zebrafish primordial germ cell migration by protecting small GTPase Cdc42

In many lower animals,germ cell formation,migration,and maintenance depend on maternally provided determinants in germ plasm.In zebrafish,these processes have been extensively studied in terms of RNA-binding proteins and other coding genes.The role of small non-coding RNAs in the regulation of primordial germ cell(PGC)development remains largely unknown and poorly investigated,even though growing interests for the importance of miRNAs involved in a wide variety of biological processes.Here,we reported the role and mechanism of the germ plasm-specific miRNA miR-202-5p in PGC migration:(i)both maternal loss and knockdown of miR-202-5p impaired PGC migration indicated by the mislocalization and reduced number of PGCs;(ii)cdc42se1 was a direct target gene of miR-202-5p,and overexpression of Cdc42se1 in PGCs caused PGC migration defects similar to those observed in loss of miR-202-5p mutants;(iii)Cdc42se1 not only interacted with Cdc42 but also inhibited cdc42 transcription,and overexpression of Cdc42 could rescue PGC migration defects in Cdc42se1 overexpressed embryos.Thus,miR-202-5p regulates PGC migration by directly targeting and repressing Cdc42se1 to protect the expression of Cdc42,which interacts with actin to direct PGC migration.

大城市郊区流动人口“带眷迁移”特征研究——基于上海市闵行区流动人口状况调查

通过分析流动人口"带眷迁移"的活跃性和迁移量,本文试图解读大城市流动人口"带眷迁移"行为的一般规律。借助于2005年7月《闵行区流动人口状况与服务需求》课题的问卷调查资料,从流动人口的来沪时间、年龄、收入水平、职业状况、受教育程度五个方面考察流动人口个体带动迁移行为发生概率及带动迁移能力。结果表明,"带眷迁移"行为受上述五方面的影响显著,年龄40岁、收入3000元成为流动人口带动迁移强度提升的节点;流动人口对少儿和老人的带动有明显差异,反映为少儿的高带动率、低带动能力,老人的低带动率、高带动能力。

大城市流动人口“带动迁移”特征分析——以上海市闵行区为例

20世纪90年代中期以来,伴随着流动人口大量涌入城市,被带动迁入的少年儿童和老人规模也日益增大,对流入地和流出地均产生一定的影响。借助问卷调查资料,以带动迁移主体为研究对象,在界定带动迁移率的基础上,从流动人口的年龄、来沪时间长短、受教育程度、从业状况、收入水平五个方面考察带动迁移行为发生概率及带动迁移能力,以期解析大城市带动迁移行为的一般特征。

西北地区进城农民工配偶相携迁移发展机制研究——基于22省60市的问卷调查

家庭迁移已经成为中国20世纪90年代以来农村劳动力乡城流动中的一个重要特征。运用课题组"城镇打工农民就业变化与市民化问卷调查"数据,分析了西北地区携眷迁移发展状况,从整体携眷迁移和夫妻不同先行者携眷迁移两个维度建立三个回归模型,将人力资本和社会资本放在统一的分析框架中,探讨西北地区进城农民工携眷迁移发展机制,首次对城市农民工配偶相携迁移机制进行了实证分析,拓展了以往农民工家庭迁移的研究视野。研究表明:西北地区未婚和已婚农民工携眷迁移水平远远低于个体迁移,且女性携眷迁移水平较男性携眷发展更低,与西北外地区携眷发展还有差距。人力资本、社会资本及城市门槛对夫妻不同先行者携眷迁移的影响作用及路径有显著差异,西北地区夫携迁移发展机制主要来自城市打工生活时间、现城镇住房状况、业缘朋友数量等级、迁移距离的作用,而妻携迁移发展的主要渠道和作用较夫携迁移多元化。应努力创建农民工相应迁移机制发挥效应的环境和平台,以增强其家庭成员迁移的人力资本、社会资本及跨越城市迁移门槛的能力,并形成携眷迁移与女性职业提升协同发展的互动机制。

Crust and upper mantle structures beneath Northeast China from receiver function studies

P-wave and S-wave receiver function analyses have been performed along a profile consisted of 27 broadband seismic stations to image the crustal and upper mantle discontinuities across Northeast China. The results show that the average Moho depth varies from about 37 km beneath the Daxing＇anling orogenic belt in the west to about 33 km beneath the Songliao Basin, and to about 35 km beneath the Changbai mountain region in the east. Our results reveal that the Moho is generally flat beneath the Daxing＇anling region and a remarkable Moho offset （about 4 km） exists beneath the basin-mountain boundary, the Daxing＇anling-Taihang Gravity Line. Beneath the Tanlu faults zone, which seperates the Songliao Basin and Changbai region, the Moho is uplift and the crustal thick- ness changes rapidly. We interpret this feature as that the Tanlu faults might deeply penetrate into the upper mantle, and facilitate the mantle upwelling along the faults during the Cenozoic era. The average depth of the lithosphereasthenosphere boundary （LAB） is - 80 km along the profile which is thinner than an average thickness of a continental lithosphere. The LAB shows an arc-like shape in the basin, with the shallowest part approximately beneath the center of the basin. The uplift LAB beneath the basin might be related to the extensive lithospheric stretching in the Mesozoic. In the mantle transition zone, a structurally complicated 660 km discontinuity with a maximum 35 km depression beneath the Changbai region is observed. The 35 km depression is roughly coincident with the location of the stagnant western pacific slab on top of the 660 km discontinuity revealed by the recent P wave tomography.

关于管线排迁工作在城市隧道建设中的思考

沈阳市南北快速干道工程南段隧道,处于交通繁忙的城市主干道上,两边建筑紧贴道路红线。地下管线密集,管线排迁工作给隧道基坑及主体结构设计、施工带来了较大的困难。通过对南段隧道管线排迁进行分析和总结,提出了相应的思路和对策,较好地解决了管线排迁、交通疏解、附属结构设置等问题,可供类似工程参考。

Late-stage rapid accumulation of the PL19-3 giant oilfield in an active fault zone during Neotectonism in the Bozhong depression,Bohai Bay

Late-stage is defined here as the period when Neotectonism occurred since 5.1 Ma. Most petroliferous basins in China lie in the areas where Neotectonism occurred intensively. In recent years, Chinese petroleum geologists have paid much attention to late-stage petroleum accumulation. The PL19-3 giant oilfield is situated where faulting activities occurred violently during Neotectonism. To understand the mechanism of lat-stage rapid accumulation, we discussed the most important aspects responsible for the formation of the giant oilfield, including oil generation, active oil-source rock occurrence, fault activity and fault conduits, late-stage rapid oil injection as well as the distinguishing indicators. This study shows that: (1) sufficient oil was supplied to the PL19-3 field since 5.1 Ma because the PL19-3 structure was surrounded by four sags in which three intervals of high-quality source rocks remained active during Neotectonism; (2) densely distributed faults and high porosity/permeability sandstone carrier beds comprised the effective conduit system for oil migration and injection; (3) oil migrated along the faults and charged the PL19-3 structure rapidly by means of seismic pumping which was triggered by frequent earthquakes during Neotetonism. It is documented that elevated reservoir temperature, abnormal geothermal gradients and abnormally high homogenization temperatures of the fluid inclusions are the indicators for late-stage rapid oil accumulation.

Mex3a promotes oncogenesis through the RAP1/MAPK signaling pathway in colorectal cancer and is inhibited by hsa-miR-6887-3p

Background:Although Mex3 RNA-binding family member A(Mex3a)has demonstrated an important role in multiple cancers,its role and regulatory mechanism in CRC is unclear.In this study,we aimed to investigate the role and clinical significance of Mex3a in CRC and to explore its underlying mechanism.Methods:Western blotting and quantitative real-time polymerase chain reaction(qRT-PCR)were performed to detect the expression levels of genes.5-Ethynyl-2’-deoxyuridine(EDU)and transwell assays were utilized to examine CRC cell proliferation and metastatic ability.The R software was used to do hierarchical clustering analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis.Overexpression and rescue experiments which included U0126,a specific mitogen activated protein kinase kinase/extracellular regulated protein kinase(MEK/ERK)inhibitor,and PX-478,a hypoxia-inducible factor 1 subunit alpha(HIF-1α)inhibitor,were used to study the molecularmechanisms of Mex3a in CRC cells.Co-immunoprecipitation(Co-IP)assay was performed to detect the interaction between two proteins.Bioinformatics analysis including available public database and Starbase software(starbase.sysu.edu.cn)were used to evaluate the expression and prognostic significance of genes.TargetScan(www.targetscan.org)and the miRDB(mirdb.org)website were used to predict the combination site between microRNA and target mRNA.BALB/c nude micewere used to study the function of Mex3a and hsa-miR-6887-3p in vivo.Results:Clinicopathological and immunohistochemical(IHC)studies of 101 CRC tissues and 79 normal tissues demonstrated that Mex3a was a significant prognostic factor for overall survival(OS)in CRC patients.Mex3a knockdown substantially inhibited the migration,invasion,and proliferation of CRC cells.Transcriptome analysis and mechanism verification showed that Mex3a regulated the RAP1 GTPase activating protein(RAP1GAP)/MEK/ERK/HIF-1αpathway.Furthermore,RAP1GAP was identified to interact with Mex3a in Co-IP experiments.Bioinformatics and dual-luciferase reporter experiments revealed that hsa-miR-6887-3p could bind to the 3’-untranslated regions(3’-UTR)of the Mex3amRNA.hsa-miR-6887-3p downregulated Mex3a expression and inhibited the tumorigenesis of CRC both in vitro and in vivo.Conclusions:Our study demonstrated that the hsa-miR-6887-3p/Mex3a/RAP1GAP signaling axis was a key regulator of CRC and Mex3a has the potential to be a new diagnostic marker and treatment target for CRC.

Reservoir forming conditions and key exploration technologies of Lingshui 17-2 giant gas field in deepwater area of Qiongdongnan Basin

On September 15,2014,China National Offshore Oil Co.,Ltd announced that a high production of oil and gas flow of 1.6
106 m3/d was obtained in Well LS17-2-1 in deepwater area in northern South China Sea,which is the first great oil and gas discovery for self-run deepwater exploration in China sea areas,and a strategic breakthrough was made in natural gas exploration in deepwater area of Lingshui sag in Qiongdongnan Basin.Under the combined action of climax of international deepwater exploration,high oil prices,national demands of China,practical needs of exploration,breakthroughs in seismic exploration and testing technologies,innovations in geological cognition and breakthroughs in deepwater operation equipment,Lingshui 17-2 gas field is discovered.Among these factors,the innovation in reservoir forming geological cognition directly promotes the discovery.The quality of seismic data in the early time is poor,so key reservoir forming conditions such as effective source rocks,high quality reservoirs and oil-gas migration pathways are unable to be ascertained;with support of new seismic acquisition and processing technology,some researches show that Lingshui sag is a successive large and deep sag with an area of 5000 km2 and the maximum thickness of Cenozoic stratum of 13 km.In the Early Oligocene,the Lingshui sag was a semi-closed delta-estuarine environment,where the coalmeasure and marine mudstones in Lower Oligocene Yacheng Formation were developed.The Lingshui sag is a sag with high temperature,and the bottom temperature of source rocks in Yacheng Formation can exceed 250C,but the simulation experiment of hydrocarbon generation at high temperature indicates that the main part of this set of source rock is still in the gas-generation window,with resources of nearly 1 trillion cubic meters,so the Lingshui sag is a hydrocarbon-rich generation sag.In the Neogene,the axial canyon channel from the Thu Bon River in Vietnam passed through the Lingshui sag,and five stages of secondary channels were developed in the axial canyon channel,where four types of reservoirs with excellent physical properties including the axial sand,lateral accretion sand,natural levee sand as well as erosion residual sand were developed,and lithologic traps or structural-lithologic traps were formed.The diapiric zone in the southern Lingshui sag connects deep source rocks in Yacheng Formation and shallow sandstones in the channels,and the migration pattern of natural gas is a T-type migration pattern,in other words,the natural gas generated from Yacheng Formation migrates vertically to the interior of the channel sandbody,and then migrates laterally in the channel reservoirs and forms the reservoirs.Innovations of geophysical exploration technologies for complicated geological conditions of deepwater areas are made,such as the detuning comprehensive quantitative amplitude hydrocarbon detection technology,which greatly improves the success rate of deepwater exploration;key technologies of deepwater safety exploratory well testing represented by the platform-dragged riser displacement technology are developed,which greatly reduces the drilling test cost.The above key exploration technologies provide a strong guarantee for the efficient exploration and development of Lingshui gas field.

MiRNA 24-3p-rich exosomes functionalized DEGMA-modified hyaluronic acid hydrogels for corneal epithelial healing

The damage of corneal epithelium may lead to the formation of irreversible corneal opacities and even blindness.The migration rate of corneal epithelial cells directly affects corneal repair.Here,we explored ocu-microRNA 24-3p(miRNA 24-3p)that can promote rabbit corneal epithelial cells migration and cornea repair.Exosomes,an excellent transport carrier,were exacted from adipose derived mesenchymal stem cells for loading with miRNA 24-3p to prepare miRNA 24-3p-rich exosomes(Exos-miRNA 24-3p).It can accelerate corneal epithelial migration in vitro and in vivo.For application in cornea alkali burns,we further modified hyaluronic acid with di(ethylene glycol)monomethyl ether methacrylate(DEGMA)to obtain a thermosensitive hydrogel,also reported a thermosensitive DEGMA-modified hyaluronic acid hydrogel(THH)for the controlled release of Exos-miRNA 24-3p.It formed a highly uniform and clear thin layer on the ocular surface to resist clearance from blinking and extended the drug-ocular-epithelium contact time.The use of THH-3/Exos-miRNA 24-3p for 28 days after alkali burn injury accelerated corneal epithelial defect healing and epithelial maturation.It also reduced corneal stromal fibrosis and macrophage activation.MiRNA 24-3p-rich exosomes functionalized DEGMA-modified hyaluronic acid hydrogel as a multilevel delivery strategy has a potential use for cell-free therapy of corneal epithelial regeneration.