Potato late blight, caused by the oomycete pathogen Phytophthora infestans, is the most serious disease of potato worldwide. The adoption of varieties with resistance genes, especially broad-spectrum resistance genes,...Potato late blight, caused by the oomycete pathogen Phytophthora infestans, is the most serious disease of potato worldwide. The adoption of varieties with resistance genes, especially broad-spectrum resistance genes, is the most efficient approach to control late blight. Solanum demissum is a well-known wild potato species from which 11 race-specific resistance genes have been identified, however, no broad-spectrum resistance genes like RB have been reported in this species. Here, we report a novel reisistance locus from S. demissum that potentially confer broad-spectrum resistance to late blight. A small segregating population of S. demissum were assessed for resistance to aggressive P. infestans isolates(race 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11). This coupled with nucleotide binding site(NBS) profiling analyses, led to the identification of three fragments that linked to the potential candidate resistance gene(s). Cloning and sequence analysis of these fragments suggested that the identified resistance gene locus is located in the region containing R2 resistance gene at chromosome 4. Based on the sequences of the cloned fragments, a co-segregating sequence characterized amplified region(SCAR) marker, RDSP, was developed. The newly identified marker RDSP will be useful for marker assisted breeding and further cloning of this potential resistance gene locus.展开更多
With the increasing maritime activities and the rapidly developing maritime economy, the fifth-generation(5G) mobile communication system is expected to be deployed at the ocean. New technologies need to be explored t...With the increasing maritime activities and the rapidly developing maritime economy, the fifth-generation(5G) mobile communication system is expected to be deployed at the ocean. New technologies need to be explored to meet the requirements of ultra-reliable and low latency communications(URLLC) in the maritime communication network(MCN). Mobile edge computing(MEC) can achieve high energy efficiency in MCN at the cost of suffering from high control plane latency and low reliability. In terms of this issue, the mobile edge communications, computing, and caching(MEC3) technology is proposed to sink mobile computing, network control, and storage to the edge of the network. New methods that enable resource-efficient configurations and reduce redundant data transmissions can enable the reliable implementation of computing-intension and latency-sensitive applications. The key technologies of MEC3 to enable URLLC are analyzed and optimized in MCN. The best response-based offloading algorithm(BROA) is adopted to optimize task offloading. The simulation results show that the task latency can be decreased by 26.5’ ms, and the energy consumption in terminal users can be reduced to 66.6%.展开更多
基金supported by the Key Laboratory of Biology and Genetic Improvement of Tuber and Root Crop, Ministry of Agriculture, P.R.Chinafunded by the National Natural Science Foundation of China (NSFC, 31000738)
文摘Potato late blight, caused by the oomycete pathogen Phytophthora infestans, is the most serious disease of potato worldwide. The adoption of varieties with resistance genes, especially broad-spectrum resistance genes, is the most efficient approach to control late blight. Solanum demissum is a well-known wild potato species from which 11 race-specific resistance genes have been identified, however, no broad-spectrum resistance genes like RB have been reported in this species. Here, we report a novel reisistance locus from S. demissum that potentially confer broad-spectrum resistance to late blight. A small segregating population of S. demissum were assessed for resistance to aggressive P. infestans isolates(race 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11). This coupled with nucleotide binding site(NBS) profiling analyses, led to the identification of three fragments that linked to the potential candidate resistance gene(s). Cloning and sequence analysis of these fragments suggested that the identified resistance gene locus is located in the region containing R2 resistance gene at chromosome 4. Based on the sequences of the cloned fragments, a co-segregating sequence characterized amplified region(SCAR) marker, RDSP, was developed. The newly identified marker RDSP will be useful for marker assisted breeding and further cloning of this potential resistance gene locus.
基金the National S&T Major Project (No. 2018ZX03001011)the National Key R&D Program(No.2018YFB1801102)+1 种基金the National Natural Science Foundation of China (No. 61671072)the Beijing Natural Science Foundation (No. L192025)
文摘With the increasing maritime activities and the rapidly developing maritime economy, the fifth-generation(5G) mobile communication system is expected to be deployed at the ocean. New technologies need to be explored to meet the requirements of ultra-reliable and low latency communications(URLLC) in the maritime communication network(MCN). Mobile edge computing(MEC) can achieve high energy efficiency in MCN at the cost of suffering from high control plane latency and low reliability. In terms of this issue, the mobile edge communications, computing, and caching(MEC3) technology is proposed to sink mobile computing, network control, and storage to the edge of the network. New methods that enable resource-efficient configurations and reduce redundant data transmissions can enable the reliable implementation of computing-intension and latency-sensitive applications. The key technologies of MEC3 to enable URLLC are analyzed and optimized in MCN. The best response-based offloading algorithm(BROA) is adopted to optimize task offloading. The simulation results show that the task latency can be decreased by 26.5’ ms, and the energy consumption in terminal users can be reduced to 66.6%.