Planting grass and legume mixtures on improved grasslands has the potential advantage of realizing both higher yields and lower environmental pollution by optimizing the balance between applied N fertilizer and the na...Planting grass and legume mixtures on improved grasslands has the potential advantage of realizing both higher yields and lower environmental pollution by optimizing the balance between applied N fertilizer and the natural process of legume biological nitrogen fixation. However, the optimal level of N fertilization for grass-legume mixtures, to obtain the highest yield, quality, and contribution of N2 fixation, varies with species. A greenhouse pot experiment was conducted to study the temporal dynamics of N2 fixation of alfalfa （Medicago sativa L.） grown alone and in mixture with smooth bromegrass （Bromus inermis Leyss.） in response to the addition of fertilizer N. Three levels of N （0, 75, and 150 kg ha-1） were examined using 15N-labeled urea to evaluate N2 fixation via the 15N isotope dilution method. Treatments were designated NO （0.001 g per pot）, N75 （1.07 g per pot） and N150 （2.14 g per pot）. Alfalfa grown alone did not benefit from the addition of fertilizer N; dry matter was not significantly increased. In contrast, dry weight and N content of smooth bromegrass grown alone was increased significantly by N application. When grown as a mixture, smooth bromegrass biomass was increased significantly by N application, resulted in a decrease in alfalfa biomass. In addition, individual alfalfa plant dry weight （shoots＋roots） was significantly lower in the mixture than when grown alone at all N levels. Smooth bromegrass shoot and root dry weight were significantly higher when grown with alfalfa than when grown alone, regardless of N application level. When grown alone, alfalfa＇s N2 fixation was reduced with N fertilization （R2=0.9376,P=0.0057）. When grown in a mixture with smooth bromegrass, with 75 kg ha-1 of N fertilizer, the percentage of atmospheric N2 fixation contribution to total N in alfalfa （%Ndfa） had a maximum of 84.07 and 83.05% in the 2nd and 3rd harvests, respectively. Total 3-harvest %Ndfa was higher when alfalfa was grown in a mixture than when grown alone （shoots： ｜t｜=3.39, P=0.0096; root： ｜t｜=3.57, P=0.0073）. We believe this was due to smooth bromegrass being better able to absorb available soil N （due to its fibrous root system）, resulting inlower soil N availability and allowing alfalfa to develop an effective N2 fixing symbiosis prior to the 1st harvest. Once soil N levels were depleted, alfalfa was able to fix N2, resulting in the majority of its tissue N being derived from biological nitrogen fixation （BNF） in the 2nd and 3rd harvests. When grown in a mixture, with added N, alfalfa established an effective symbiosis earlier than when grown alone; in monoculture BNF did not contribute a significant portion of plant N in the N75 and N150 treatments, whereas in the mixture, BNF contributed 17.90 and 16.28% for these treatments respectively. Alfalfa has a higher BNF efficiency when grown in a mixture, initiating BNF earlier, and having higher N2 fixation due to less inhibition by soil-available N. For the greatest N-use-efficiency and sustainable production, grass-legume mixtures are recommended for imDrovino orasslands, usino a moderate amount of N fertilizer （75 kq N ha-l） to provide optimum benefits.展开更多
Fusarium sp. strain ZH-H2 is capable to degrade high molecular weight polycyclic aromatic hydrocarbons （HMW-PAHs）, smooth bromegrass （Bromus inermis Leyss.） can also degrade 4- to 6-ring PAHs. Pot experiments were...Fusarium sp. strain ZH-H2 is capable to degrade high molecular weight polycyclic aromatic hydrocarbons （HMW-PAHs）, smooth bromegrass （Bromus inermis Leyss.） can also degrade 4- to 6-ring PAHs. Pot experiments were conducted to investigate how bromegrass and different inoculum sizes of ZH-H2 clean up HMW-PAHs in agricultural soil derived from a coal mine area. The results showed that, compared with control, different sizes of inocula of ZH-H2 effectively degraded HMW-PAHs, with removal rates of 19.01, 34.25 and 29.26% for 4-, 5- and 6-ring PAHs in the treatment with 1.0 g kg-1ZH- H2 incubation after 90 d. After 5 mon of cultivation, bromegrass reached degradation rate of these compounds by 12.66, 36.26 and 36.24%, respectively. By adding strain ZH-H2 to bromegrass, HMW-PAHs degradation was further improved up to 4.24 times greater than bromegrass （W）, in addition to the degradation rate of Bbf decrease. For removal rates of both 5- and 6-ring PAHs, addition of 0.5 g kg-1 Fusarium ZH-H2 to pots with bromegrass performed better than addition of 0.1 g kg-1, while the highest concentration of 1.0 g kg-1 Fusarium ZH-H2 did not further improve degradation. Degradation of4-ring PAHs showed no significant difference among different ZH-H2 incubations with bromegrass treatments. We found that the degradation rates of 4-, 5- and 6-ring PAHs in all treatments are significantly correlated in a positive, linear man- ner with activity of lignin peroxidase （LIP） （t=0.8065, 0.9350 and 0.9165, respectively）, while degradation of 5- and 6-ring PAHs is correlated to polyphenoloxidase （PPO） activity （r=0.7577 and 07806）. Our findings suggest that the combination of Fusarium sp. ZH-H2 and bromegrass offers a suitable alternative for phytoremediation of aged PAH-contaminated soil in coal mining areas, with a recommended inoculation size of 0.5 g Fusarium sp. ZH-H2 per kg soil.展开更多
A field experiment with 24 treatments consisting of three perennial forage crops [alfalfa (<em>Medicago sativa</em> L. cv. AC Longview), hybrid bromegrass (<em>Bromus riparius</em> Rehm & &...A field experiment with 24 treatments consisting of three perennial forage crops [alfalfa (<em>Medicago sativa</em> L. cv. AC Longview), hybrid bromegrass (<em>Bromus riparius</em> Rehm & <em>Bromus inermis</em> Leyss. cv. AC Success) and their mixture], four Cut 1 dates (approximately June 20, July 10, July 30 or August 20), and two fertilizer levels (unfertilized and fertilized) was established in late May 2014, on a Black Chernozem [Udic Boroll] silty clay soil. Forage dry matter yield [DMY], and concentration (g<span style="white-space:nowrap;">·</span>kg<sup><span style="white-space:nowrap;">−</span>1</sup> DM) of crude protein [CP], total digestible nutrients [TDN] and acid detergent fiber [ADF] data were collected over 3 years from 2015 to 2017. The fertilizer treatments were imposed in 2016 and 2017. Forage crops were initially cut at four Cut 1 dates, and again cut [Cut 2] in autumn (September 2 in 2015, November 7 in 2016 and October 5 in 2017). For all three forage crops, forage DMY usually increased when Cut 1 was delayed. Delaying Cut 1 reduced forage DMY for Cut 2. Total DMY (Cut 1 + Cut 2) for all three forage crops was highest from the combination of July 10 and late Autumn cuts. Alfalfa-bromegrass mixture produced higher DMY than bromegrass or alfalfa alone. Fertilizer application resulted in a significant increase in Cut 1 and total DMY for bromegrass. The CP concentration in Cut 1 forage usually declined as the forage crops matured. The CP concentration was highest for alfalfa, followed by alfalfa-bromegrass mixture, and much lower for bromegrass. There was little or no effect of forage crop maturity on the TDN and ADF concentrations in forage. The TDN concentration was higher and ADF concentration was lower in forage from alfalfa or alfalfa-bromegrass mixture than bromegrass. Fertilizer application significantly increased CP concentration for alfalfa-bromegrass mixture. Delaying harvesting for Cut 1 increased ADF yield and TDN yield until Late July, but CP yield generally decreased with crop maturity. The ADF yield and TDN yield were higher for alfalfa-bromegrass mixture than bromegrass or alfalfa alone, and CP yield was similar for alfalfa and alfalfa-bromegrass mixture but considerably higher than bromegrass. Fertilizer application increased CP yield and ADF yield for bromegrass and alfalfa-bromegrass mixture, and TDN yield only for bromegrass. In conclusion, total DMY (Cut 1 + Cut 2) was highest for a combination of Early July and Autumn cuts. Forage yield was highest for alfalfa-bromegrass mixture, followed by alfalfa and lowest for bromegrass. The CP and TDN concentrations were higher, and ADF concentrations were lower in forage from alfalfa or alfalfa-bromegrass mixture than bromegrass.展开更多
Meadow bromegrass (Bromus riparius Rehmann) is an important forage crop in Canada and the Intermountain West, but it has not been extensively evaluated in the northern Great Plains (NGP). Our objectives were to 1) eva...Meadow bromegrass (Bromus riparius Rehmann) is an important forage crop in Canada and the Intermountain West, but it has not been extensively evaluated in the northern Great Plains (NGP). Our objectives were to 1) evaluate meadow, smooth, and hybrid bromegrasses for forage production under intensive management in eastern South Dakota, and 2) evaluate the effect of selection for vigor in multiple environments on forage production of meadow bromegrass. Thirteen populations (7 meadow bromegrass, 5 hybrid bromegrass, and 1 smooth bromegrass) were evaluated for 4 yrs (2005-2007, 2009) at Brookings, SD. Biomass at anthesis was greater for smooth (6.75 Mg ha-1) than for meadow or hybrid brome (5.4 Mg ha-1) in 2005, but production at anthesis during 2006 and 2007 was similar for meadow and smooth bromegrass. Regrowth harvested during July and October 2005 and November 2006 was greater for meadow than smooth or hybrid bromegrass. Forage production at anthesis in 2009, after rest (i.e., no cutting) and fertilization in 2008, was 4.2 Mg ha-1 for meadow bromegrass compared with 3.3 Mg ha-1 and 2.6 Mg ha-1 for hybrid and smooth bromegrass, respectively. Smooth and hybrid bromegrasses had more leaves·tiller-1 than meadow bromegrass. After 6 yrs, meadow bromegrass had higher tiller density and greater potential for tolerating multiple harvests during a growing season than did smooth or hybrid bromegrass. Selection for vigor in multiple environments in North America resulted in experimental populations of meadow bromegrass with superior forage yield compared with ‘Fleet’ on marginal crop land in the northern Great Plains.展开更多
Beef cattle (Bos taurus L.) feedlots pose serious environmental challenges associated with nutrient runoff. Smooth bromegrass (Bromus inermis Leyss.) is a perennial rhizomatous grass that is widely used for forage pro...Beef cattle (Bos taurus L.) feedlots pose serious environmental challenges associated with nutrient runoff. Smooth bromegrass (Bromus inermis Leyss.) is a perennial rhizomatous grass that is widely used for forage production in the USA and Canada. The objective of this research was to determine the best management system for producing forage from a vegetated treatment area (VTA) while maintaining the capacity of the VTA to remove nutrients from feedlot effluent. Four harvest management treatments (1-, 2-, and 3-harvest per year and an un-harvested control) were applied during spring 2005 and evaluated over a 5-yr period in a smooth brome sward on a VTA near Howard, SD. Mean annual total forage production during a 4-yr period ranged from 6.2 Mg·ha-1 to 9.5 Mg·ha-1 for 1- and 3-harvest systems, respectively. Nutrient removal by the bromegrass ranged from 148 kg·N·ha-1 and 15 kg·P·ha-1 for the 1-harvest treatment to 244 kg·ha-1 N and 24 kg·ha-1 P for the 3-harvest treatment. Results indicated that high amounts of smooth bromegrass forage could be produced and soil nutrients removed from 2- and 3-harvest systems without compro-mising stand vigor and persistence on a VTA in eastern South Dakota.展开更多
基金supported by the China Forage and Grass Research System (CARS-35)the National Key Technology R&D Program of China (2011BAD17B01)
文摘Planting grass and legume mixtures on improved grasslands has the potential advantage of realizing both higher yields and lower environmental pollution by optimizing the balance between applied N fertilizer and the natural process of legume biological nitrogen fixation. However, the optimal level of N fertilization for grass-legume mixtures, to obtain the highest yield, quality, and contribution of N2 fixation, varies with species. A greenhouse pot experiment was conducted to study the temporal dynamics of N2 fixation of alfalfa （Medicago sativa L.） grown alone and in mixture with smooth bromegrass （Bromus inermis Leyss.） in response to the addition of fertilizer N. Three levels of N （0, 75, and 150 kg ha-1） were examined using 15N-labeled urea to evaluate N2 fixation via the 15N isotope dilution method. Treatments were designated NO （0.001 g per pot）, N75 （1.07 g per pot） and N150 （2.14 g per pot）. Alfalfa grown alone did not benefit from the addition of fertilizer N; dry matter was not significantly increased. In contrast, dry weight and N content of smooth bromegrass grown alone was increased significantly by N application. When grown as a mixture, smooth bromegrass biomass was increased significantly by N application, resulted in a decrease in alfalfa biomass. In addition, individual alfalfa plant dry weight （shoots＋roots） was significantly lower in the mixture than when grown alone at all N levels. Smooth bromegrass shoot and root dry weight were significantly higher when grown with alfalfa than when grown alone, regardless of N application level. When grown alone, alfalfa＇s N2 fixation was reduced with N fertilization （R2=0.9376,P=0.0057）. When grown in a mixture with smooth bromegrass, with 75 kg ha-1 of N fertilizer, the percentage of atmospheric N2 fixation contribution to total N in alfalfa （%Ndfa） had a maximum of 84.07 and 83.05% in the 2nd and 3rd harvests, respectively. Total 3-harvest %Ndfa was higher when alfalfa was grown in a mixture than when grown alone （shoots： ｜t｜=3.39, P=0.0096; root： ｜t｜=3.57, P=0.0073）. We believe this was due to smooth bromegrass being better able to absorb available soil N （due to its fibrous root system）, resulting inlower soil N availability and allowing alfalfa to develop an effective N2 fixing symbiosis prior to the 1st harvest. Once soil N levels were depleted, alfalfa was able to fix N2, resulting in the majority of its tissue N being derived from biological nitrogen fixation （BNF） in the 2nd and 3rd harvests. When grown in a mixture, with added N, alfalfa established an effective symbiosis earlier than when grown alone; in monoculture BNF did not contribute a significant portion of plant N in the N75 and N150 treatments, whereas in the mixture, BNF contributed 17.90 and 16.28% for these treatments respectively. Alfalfa has a higher BNF efficiency when grown in a mixture, initiating BNF earlier, and having higher N2 fixation due to less inhibition by soil-available N. For the greatest N-use-efficiency and sustainable production, grass-legume mixtures are recommended for imDrovino orasslands, usino a moderate amount of N fertilizer （75 kq N ha-l） to provide optimum benefits.
基金supported by the National High-Tech R&D Program of China(863 Program)(2012AA101403)the Educational Commission of Hebei Province of China(Z2013058)+1 种基金the Human Resources Department of Hebei Province of China(2013–2016 Project)the Educational Commission of Hebei Province of China(ZD2013013)
文摘Fusarium sp. strain ZH-H2 is capable to degrade high molecular weight polycyclic aromatic hydrocarbons （HMW-PAHs）, smooth bromegrass （Bromus inermis Leyss.） can also degrade 4- to 6-ring PAHs. Pot experiments were conducted to investigate how bromegrass and different inoculum sizes of ZH-H2 clean up HMW-PAHs in agricultural soil derived from a coal mine area. The results showed that, compared with control, different sizes of inocula of ZH-H2 effectively degraded HMW-PAHs, with removal rates of 19.01, 34.25 and 29.26% for 4-, 5- and 6-ring PAHs in the treatment with 1.0 g kg-1ZH- H2 incubation after 90 d. After 5 mon of cultivation, bromegrass reached degradation rate of these compounds by 12.66, 36.26 and 36.24%, respectively. By adding strain ZH-H2 to bromegrass, HMW-PAHs degradation was further improved up to 4.24 times greater than bromegrass （W）, in addition to the degradation rate of Bbf decrease. For removal rates of both 5- and 6-ring PAHs, addition of 0.5 g kg-1 Fusarium ZH-H2 to pots with bromegrass performed better than addition of 0.1 g kg-1, while the highest concentration of 1.0 g kg-1 Fusarium ZH-H2 did not further improve degradation. Degradation of4-ring PAHs showed no significant difference among different ZH-H2 incubations with bromegrass treatments. We found that the degradation rates of 4-, 5- and 6-ring PAHs in all treatments are significantly correlated in a positive, linear man- ner with activity of lignin peroxidase （LIP） （t=0.8065, 0.9350 and 0.9165, respectively）, while degradation of 5- and 6-ring PAHs is correlated to polyphenoloxidase （PPO） activity （r=0.7577 and 07806）. Our findings suggest that the combination of Fusarium sp. ZH-H2 and bromegrass offers a suitable alternative for phytoremediation of aged PAH-contaminated soil in coal mining areas, with a recommended inoculation size of 0.5 g Fusarium sp. ZH-H2 per kg soil.
文摘A field experiment with 24 treatments consisting of three perennial forage crops [alfalfa (<em>Medicago sativa</em> L. cv. AC Longview), hybrid bromegrass (<em>Bromus riparius</em> Rehm & <em>Bromus inermis</em> Leyss. cv. AC Success) and their mixture], four Cut 1 dates (approximately June 20, July 10, July 30 or August 20), and two fertilizer levels (unfertilized and fertilized) was established in late May 2014, on a Black Chernozem [Udic Boroll] silty clay soil. Forage dry matter yield [DMY], and concentration (g<span style="white-space:nowrap;">·</span>kg<sup><span style="white-space:nowrap;">−</span>1</sup> DM) of crude protein [CP], total digestible nutrients [TDN] and acid detergent fiber [ADF] data were collected over 3 years from 2015 to 2017. The fertilizer treatments were imposed in 2016 and 2017. Forage crops were initially cut at four Cut 1 dates, and again cut [Cut 2] in autumn (September 2 in 2015, November 7 in 2016 and October 5 in 2017). For all three forage crops, forage DMY usually increased when Cut 1 was delayed. Delaying Cut 1 reduced forage DMY for Cut 2. Total DMY (Cut 1 + Cut 2) for all three forage crops was highest from the combination of July 10 and late Autumn cuts. Alfalfa-bromegrass mixture produced higher DMY than bromegrass or alfalfa alone. Fertilizer application resulted in a significant increase in Cut 1 and total DMY for bromegrass. The CP concentration in Cut 1 forage usually declined as the forage crops matured. The CP concentration was highest for alfalfa, followed by alfalfa-bromegrass mixture, and much lower for bromegrass. There was little or no effect of forage crop maturity on the TDN and ADF concentrations in forage. The TDN concentration was higher and ADF concentration was lower in forage from alfalfa or alfalfa-bromegrass mixture than bromegrass. Fertilizer application significantly increased CP concentration for alfalfa-bromegrass mixture. Delaying harvesting for Cut 1 increased ADF yield and TDN yield until Late July, but CP yield generally decreased with crop maturity. The ADF yield and TDN yield were higher for alfalfa-bromegrass mixture than bromegrass or alfalfa alone, and CP yield was similar for alfalfa and alfalfa-bromegrass mixture but considerably higher than bromegrass. Fertilizer application increased CP yield and ADF yield for bromegrass and alfalfa-bromegrass mixture, and TDN yield only for bromegrass. In conclusion, total DMY (Cut 1 + Cut 2) was highest for a combination of Early July and Autumn cuts. Forage yield was highest for alfalfa-bromegrass mixture, followed by alfalfa and lowest for bromegrass. The CP and TDN concentrations were higher, and ADF concentrations were lower in forage from alfalfa or alfalfa-bromegrass mixture than bromegrass.
文摘Meadow bromegrass (Bromus riparius Rehmann) is an important forage crop in Canada and the Intermountain West, but it has not been extensively evaluated in the northern Great Plains (NGP). Our objectives were to 1) evaluate meadow, smooth, and hybrid bromegrasses for forage production under intensive management in eastern South Dakota, and 2) evaluate the effect of selection for vigor in multiple environments on forage production of meadow bromegrass. Thirteen populations (7 meadow bromegrass, 5 hybrid bromegrass, and 1 smooth bromegrass) were evaluated for 4 yrs (2005-2007, 2009) at Brookings, SD. Biomass at anthesis was greater for smooth (6.75 Mg ha-1) than for meadow or hybrid brome (5.4 Mg ha-1) in 2005, but production at anthesis during 2006 and 2007 was similar for meadow and smooth bromegrass. Regrowth harvested during July and October 2005 and November 2006 was greater for meadow than smooth or hybrid bromegrass. Forage production at anthesis in 2009, after rest (i.e., no cutting) and fertilization in 2008, was 4.2 Mg ha-1 for meadow bromegrass compared with 3.3 Mg ha-1 and 2.6 Mg ha-1 for hybrid and smooth bromegrass, respectively. Smooth and hybrid bromegrasses had more leaves·tiller-1 than meadow bromegrass. After 6 yrs, meadow bromegrass had higher tiller density and greater potential for tolerating multiple harvests during a growing season than did smooth or hybrid bromegrass. Selection for vigor in multiple environments in North America resulted in experimental populations of meadow bromegrass with superior forage yield compared with ‘Fleet’ on marginal crop land in the northern Great Plains.
文摘Beef cattle (Bos taurus L.) feedlots pose serious environmental challenges associated with nutrient runoff. Smooth bromegrass (Bromus inermis Leyss.) is a perennial rhizomatous grass that is widely used for forage production in the USA and Canada. The objective of this research was to determine the best management system for producing forage from a vegetated treatment area (VTA) while maintaining the capacity of the VTA to remove nutrients from feedlot effluent. Four harvest management treatments (1-, 2-, and 3-harvest per year and an un-harvested control) were applied during spring 2005 and evaluated over a 5-yr period in a smooth brome sward on a VTA near Howard, SD. Mean annual total forage production during a 4-yr period ranged from 6.2 Mg·ha-1 to 9.5 Mg·ha-1 for 1- and 3-harvest systems, respectively. Nutrient removal by the bromegrass ranged from 148 kg·N·ha-1 and 15 kg·P·ha-1 for the 1-harvest treatment to 244 kg·ha-1 N and 24 kg·ha-1 P for the 3-harvest treatment. Results indicated that high amounts of smooth bromegrass forage could be produced and soil nutrients removed from 2- and 3-harvest systems without compro-mising stand vigor and persistence on a VTA in eastern South Dakota.
