The use of exogenous carbon monoxide releasing molecules（CORMs）provides promise for clinical application;however,the hazard potential of CORMs in vivo remains poorly understood.The developmental toxicity of CORM-3 w...The use of exogenous carbon monoxide releasing molecules（CORMs）provides promise for clinical application;however,the hazard potential of CORMs in vivo remains poorly understood.The developmental toxicity of CORM-3 was investigated by exposure to concentrations ranging from 6.25 to400μmol/L during 4-144 h post fertilization.Toxicity endpoints of mortality,spontaneous movement,heart rate,hatching rate,malformation,body length,and larval behavior were measured.展开更多
Fertilised eggs of the devil stringer （Inimicusjaponicus） were incubated at different salinity levels （21, 25, 29, 33, and 37）, and then the hatching performances, morphological parameters, and biochemical composi...Fertilised eggs of the devil stringer （Inimicusjaponicus） were incubated at different salinity levels （21, 25, 29, 33, and 37）, and then the hatching performances, morphological parameters, and biochemical composition （protein, lipid and carbohydrate） of the larvae were assayed to determine the influence of salinity on the early development ofl.japonicus. The tested salinity levels did not affect the times of hatching or mouth opening for yolk-sac larvae. However, the salinity significantly influenced the hatching and survival rates of open-mouthed larvae, as well as the morphology of yolk-sac larvae. The data indicated that 30.5 to 37.3 and 24.4 to 29.8 were suitable salinity ranges for the survival of embryos and larvae ofl.japonicus, respectively. Larvae incubated at a salinity level of 29 had the greatest full lengths, and decreasing yolk volume was positively correlated with the environmental salinity. With increasing salinity, the individual dry weights of newly hatched larvae or open-mouthed larvae decreased significantly. Newly hatched larvae incubated at a salinity level of 29 had the greatest metabolic substrate contents and gross energy levels, while the open- mouthed larvae＇s greatest values occurred at a salinity level of 25. Larvae incubated in the salinity range of 33 to 37 had the lowest nutritional reserves and energy values. Thus, the I.japonicus yolk-sac larvae acclimated more readily to the lower salinity level than the embryos, and higher salinity levels negatively influenced larval growth and development. In conclusion, the environmental salinity level should be maintained at 29-33 during embryogenesis and at 25-29 during early larval development for this species. Our results can be used to provide optimum aquaculture conditions for the early larval development of I.japonicus.展开更多
基金supported by National Basic Research Program of China(2010CB834202)the National Natural Science Foundation of China(11305226,11175222)+1 种基金the Scientific Technology Research Projects of Lanzhou City(2015-3-75)the Western Talent Program of Chinese Academy of Sciences(Y460030XB0)
文摘The use of exogenous carbon monoxide releasing molecules（CORMs）provides promise for clinical application;however,the hazard potential of CORMs in vivo remains poorly understood.The developmental toxicity of CORM-3 was investigated by exposure to concentrations ranging from 6.25 to400μmol/L during 4-144 h post fertilization.Toxicity endpoints of mortality,spontaneous movement,heart rate,hatching rate,malformation,body length,and larval behavior were measured.
基金Supported by the Innovation Project of the Shanghai Education Commission,China(No.12ZZ166)the Shanghai Universities First-Class Disciplines Project of Fisheries
文摘Fertilised eggs of the devil stringer （Inimicusjaponicus） were incubated at different salinity levels （21, 25, 29, 33, and 37）, and then the hatching performances, morphological parameters, and biochemical composition （protein, lipid and carbohydrate） of the larvae were assayed to determine the influence of salinity on the early development ofl.japonicus. The tested salinity levels did not affect the times of hatching or mouth opening for yolk-sac larvae. However, the salinity significantly influenced the hatching and survival rates of open-mouthed larvae, as well as the morphology of yolk-sac larvae. The data indicated that 30.5 to 37.3 and 24.4 to 29.8 were suitable salinity ranges for the survival of embryos and larvae ofl.japonicus, respectively. Larvae incubated at a salinity level of 29 had the greatest full lengths, and decreasing yolk volume was positively correlated with the environmental salinity. With increasing salinity, the individual dry weights of newly hatched larvae or open-mouthed larvae decreased significantly. Newly hatched larvae incubated at a salinity level of 29 had the greatest metabolic substrate contents and gross energy levels, while the open- mouthed larvae＇s greatest values occurred at a salinity level of 25. Larvae incubated in the salinity range of 33 to 37 had the lowest nutritional reserves and energy values. Thus, the I.japonicus yolk-sac larvae acclimated more readily to the lower salinity level than the embryos, and higher salinity levels negatively influenced larval growth and development. In conclusion, the environmental salinity level should be maintained at 29-33 during embryogenesis and at 25-29 during early larval development for this species. Our results can be used to provide optimum aquaculture conditions for the early larval development of I.japonicus.
文摘试验结果表明:泰山赤鳞鱼产卵时间在4～5月,自然产卵水温为18～26℃,产卵于水底细沙中,分散产卵.卵呈弱粘性,金黄色,属沉性卵,平均卵径2.5 mm.在水温18～22℃条件下,胚胎从受精到孵化出膜历时66 h,经过了胚盘形成期、卵裂期、囊胚期、原肠胚期、神经胚期、器官分化期6个主要阶段.初孵仔鱼全长6.0～6.5 mm.卵黄囊前部膨大成球形,约占鱼体整个长度的1/3;后端为均匀的棒状.仔鱼孵出到消化道完全形成约经历136 h.
