The present investigation was carried out in exfoliated buccal cells and saliva collected from pre-operative brain tumour patients. The DNA damage in these cells was assessed by alkaline comet assay and micronucleus (...The present investigation was carried out in exfoliated buccal cells and saliva collected from pre-operative brain tumour patients. The DNA damage in these cells was assessed by alkaline comet assay and micronucleus (MN) assay. Salivary flow rate, pH, osmolality, total antioxidant activity (AOA) and vitamin C levels were also assessed in unstimulated whole saliva of these patients. In the comet assay a significant increase in the tail length (P<0.02) was observed when control and malignant groups were compared. A significant (P<0.02) difference in tail length was also noted between benign and malignant groups. Non significant results were found when control and benign groups were compared. Further, a marked increase in % MN (P<0.002) was observed when control and benign groups were compared. A significant increase in % MN (P<0.029) was also observed in benign cases when compared to malignant tumours. No significance was obtained when % MN in control and malignant cases was compared. Moreover, salivary flow rate and pH was significantly decreased and osmolality was markedly increased in brain tumour patients. The AOA levels in saliva were markedly decreased in brain tumours and vitamin C levels exhibited no change when compared to controls. Thus, as noted above susceptibility to free radical induced DNA damage also exists in the exfoliated buccal cells conducive to the lowered salivary antioxidant status of brain tumour patients.展开更多
Vascular malformations occur most frequently in the maxillofacial and oral regions. Although many cases of vascular malformations with phlebolithiasis have been reported, only few reports have documented cases of thro...Vascular malformations occur most frequently in the maxillofacial and oral regions. Although many cases of vascular malformations with phlebolithiasis have been reported, only few reports have documented cases of thrombosis formation. We report a rare case of a vascular malformation with multiple thromboses in the left buccal region. A 28-year-old woman had a painless swelling in the left cheek when she consulted our hospital. T1-weighted magnetic resonance imaging revealed a low-signal lesion, having the same intensity as the muscle, in the left buccal region, and T2-weighted imaging revealed a high-signal lesion. Additionally, three tuberous tumours were noted in this lesion showing both a light high signal on T1-weighted imaging and a low signal on T2-weighted imaging. Based on these imaging findings, as well as clinical and pathological findings, the patient was diagnosed with multiple vascular malformations in the left midfacial region. The three tuberous tumours showed necrotic tissue in the central area, which was formed by calcified and concentric fibrous tissue, and vascularization. Because tumorous lesion and typical vascular structure by the Elastica Van Gieson staining were not observed, these tumours were seemed that fibrin thrombus in this lesion formed the organized tissue.展开更多
文摘The present investigation was carried out in exfoliated buccal cells and saliva collected from pre-operative brain tumour patients. The DNA damage in these cells was assessed by alkaline comet assay and micronucleus (MN) assay. Salivary flow rate, pH, osmolality, total antioxidant activity (AOA) and vitamin C levels were also assessed in unstimulated whole saliva of these patients. In the comet assay a significant increase in the tail length (P<0.02) was observed when control and malignant groups were compared. A significant (P<0.02) difference in tail length was also noted between benign and malignant groups. Non significant results were found when control and benign groups were compared. Further, a marked increase in % MN (P<0.002) was observed when control and benign groups were compared. A significant increase in % MN (P<0.029) was also observed in benign cases when compared to malignant tumours. No significance was obtained when % MN in control and malignant cases was compared. Moreover, salivary flow rate and pH was significantly decreased and osmolality was markedly increased in brain tumour patients. The AOA levels in saliva were markedly decreased in brain tumours and vitamin C levels exhibited no change when compared to controls. Thus, as noted above susceptibility to free radical induced DNA damage also exists in the exfoliated buccal cells conducive to the lowered salivary antioxidant status of brain tumour patients.
文摘Vascular malformations occur most frequently in the maxillofacial and oral regions. Although many cases of vascular malformations with phlebolithiasis have been reported, only few reports have documented cases of thrombosis formation. We report a rare case of a vascular malformation with multiple thromboses in the left buccal region. A 28-year-old woman had a painless swelling in the left cheek when she consulted our hospital. T1-weighted magnetic resonance imaging revealed a low-signal lesion, having the same intensity as the muscle, in the left buccal region, and T2-weighted imaging revealed a high-signal lesion. Additionally, three tuberous tumours were noted in this lesion showing both a light high signal on T1-weighted imaging and a low signal on T2-weighted imaging. Based on these imaging findings, as well as clinical and pathological findings, the patient was diagnosed with multiple vascular malformations in the left midfacial region. The three tuberous tumours showed necrotic tissue in the central area, which was formed by calcified and concentric fibrous tissue, and vascularization. Because tumorous lesion and typical vascular structure by the Elastica Van Gieson staining were not observed, these tumours were seemed that fibrin thrombus in this lesion formed the organized tissue.
