Tartary buckwheat(Fagopyrum tataricum)is a well-known pseudocereal for its health and economic value.However,abundant antinutritional factors(ANFs)reduces its health benefits.As reported,germination can improve the nu...Tartary buckwheat(Fagopyrum tataricum)is a well-known pseudocereal for its health and economic value.However,abundant antinutritional factors(ANFs)reduces its health benefits.As reported,germination can improve the nutritional profile of grains.In this study,we systematically evaluate the safety of Tartary buckwheat seeds(TB)and Tartary buckwheat sprouts(TBS)used as high active ingredients.After evaluating nutrition levels,bioactive compounds and ANFs in TBS during germinating,5^(th)-day TBS were selected as the raw material.C57BL/6J mice were gavaged daily with distilled water,TB,or TBS for 6 weeks.The physiological indices related to ANFs were determined.Results showed that the TB intake tends to generate negative effects on the gut microbiota,and organs.Additionally,upon TB intake,the Fe^(3+)content in serum,trypsin activity in pancreas and jejunum decreased,while the cytokine,IgE,and histamine levels in serum,water content in faeces,cytokine levels in liver and jejunum increased.Conversely,TBS did not induce any obvious negative effects on the above relevant indices and showed better lipid-lowering effect.Altogether,TBS are safer and more effective as a raw material to produce the functional food for long-term consumption with the intention of preventing and treating hyperlipidaemia.展开更多
To mitigate the wastage of seed resources and reduce the usage of pesticides and fertilizers, seed coating agentshave gained popularity. This study employs single-factor and multi-index orthogonal experimental design ...To mitigate the wastage of seed resources and reduce the usage of pesticides and fertilizers, seed coating agentshave gained popularity. This study employs single-factor and multi-index orthogonal experimental design methodsto investigate the seed coating formula and physical properties of Tartary buckwheat. The specific effects ofeach component on Tartary buckwheat seed germination are analyzed. The findings reveal that the seed coatingagent formulated with 1.5% polyvinyl alcohol, 0.15% sodium alginate, 0.2% op-10, 0.1% polyacrylamide, 8% colorant,3% ammonium sulfate, 1% potassium dihydrogen phosphate, and 0.15% carbendazim exhibits the mosteffective coating. It demonstrates optimal physical properties and promotes seed germination efficiently. The suspensionrate of this seed coating agent reaches 91.12%, with a mere 2.13% coating shedding rate and 2.5% coatingseed rot rate. Furthermore, it achieves a germination percentage of 99.17%, which is 20.84% higher than the lowestgroup. The germination potential and index are also significantly higher than the lowest group, with anincrease of 20.84% and 26.56%, respectively. Additionally, the vitality index is 553.08, a 15.75% increase comparedto the lowest group. The application of seed coating agents helps reduce seed resource loss, increase plant numbers,and ultimately enhance agricultural yields. This finding holds practical significance in agriculturalproduction.展开更多
Aluminum (Al) toxicity is a considerable factor limiting crop yield and biomass in acidic soil. Tartary buckwheatgrowing in acidic soil may suffer from Al poisoning. Here, we investigated the influence of Al stress on...Aluminum (Al) toxicity is a considerable factor limiting crop yield and biomass in acidic soil. Tartary buckwheatgrowing in acidic soil may suffer from Al poisoning. Here, we investigated the influence of Al stress on the growthof tartary buckwheat seedling roots, and the alleviation of Al stress by silicon (Si), as has been demonstrated inmany crops. Under Al stress, root growth (total root length, primary root length, root tips, root surface area, androot volume) was significantly inhibited, and Al and malondialdehyde (MDA) accumulated in the root tips. At thesame time, catalase (CAT) and ascorbate peroxidase activities, polyphenols, flavonoids, and 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free-radical scavenging abilitywere significantly decreased. After the application of Si, root growth, Al accumulation, and oxidative damage wereimproved. Compared to Al-treated seedlings, the contents of ·O2− and MDA decreased by 29.39% and 25.22%,respectively. This was associated with Si-induced increases in peroxidase and CAT enzyme activity, flavonoidcompounds, and free-radical scavenging (DPPH and ABTS). The application of Si therefore has positive effectson Al toxicity in tartary buckwheat roots by reducing Al accumulation in the roots and maintaining oxidationhomeostasis.展开更多
Diabetes is one of the most difficult chronic diseases to cure in the world,which seriously affects people’s health and quality of life.Flavonoids in buckwheat can regulate blood glucose levels by inhibitingα-amylas...Diabetes is one of the most difficult chronic diseases to cure in the world,which seriously affects people’s health and quality of life.Flavonoids in buckwheat can regulate blood glucose levels by inhibitingα-amylase activity.Therefore,sweet buckwheat produced in Inner Mongolia was used as the research object,and buckwheat fl avonoids were extracted by ultrasonic-assisted extraction method.Total fl avonoids content was determined by ultraviolet-visible spectrophotometry.With acarbose as the positive control,the inhibition test ofα-amylase was carried out by DNS colorimetry to study the inhibition behavior of fl avonoids onα-amylase activity.The results showed that the extraction process of flavonoids was stable and reliable,and the established method for the determination of flavonoids was simple,accurate and reproducible.The total flavonoids content of buckwheat samples was 2.706 mg/g,buckwheat total fl avonoids extraction solution had an inhibitory eff ect onα-amylase,and its median inhibition concentration(IC_(50))was 38.53 mg/mL.The results of this experiment provide a technical reference for the development and utilization of fl avonoids in Inner Mongolia sweet buckwheat,and provide a theoretical reference for the development and application of flavonoid-rich hypoglycemic food.展开更多
[Objective]The study aimed to carry out RAPD analysis of genetic diversity of common and tartary buckwheat varieties.[Method] The genetic diversity of 19 common and tartary buckwheat varieties including the tested var...[Objective]The study aimed to carry out RAPD analysis of genetic diversity of common and tartary buckwheat varieties.[Method] The genetic diversity of 19 common and tartary buckwheat varieties including the tested varieties in Guizhou region during 1999-2010 and their parents were studied using 7 primers by means of random amplified polymorphic DNA（RAPD）.[Result]A total of 149 DNA bands were obtained.In which,141 bands were polymorphic,accounting for 94.89%.Polymorphism analysis and cluster analysis showed that all varieties had their own special bands different from each other.The varieties native to Weining were close to each other,and other common buckwheat varieties were obviously different from each other.The interspecific genetic variation was the greatest;the intraspecific genetic variation of common buckwheat varieties was greater than that of tartary buckwheat varieties.[Conclusion]The RAPD fingerprints of the 19 buckwheat varieties were established in present study.展开更多
[Objective] This study aimed to finding out the difference in glucose content of tartary buckwheat seeds from different origins.[Method] Taking 35 kinds of tartary buckwheat from different origins as the test material...[Objective] This study aimed to finding out the difference in glucose content of tartary buckwheat seeds from different origins.[Method] Taking 35 kinds of tartary buckwheat from different origins as the test materials,the glucose content of seeds had been determined.[Result] The variation range of glucose content in 35 kinds of tartary buckwheat was from 0.055 6% to 0.840 2% with an average of 0.321 7%.There was a difference in glucose content of tartary buckwheat from different origins.The seeds T324 from Weining in Guizhou had the highest glucose content,while the seeds T398 also from Weining in Guizhou had the lowest glucose content.[Conclusion] This study provides a reference for the genetic variation law of glucose content in different tartary buckwheat resources.展开更多
[Objective]The aim was to select tartary buckwheat materials with high protein content.[Method]Used 35 kinds of tartary buckwheat as experimental material,and the protein content of seeds had been determined.[Result]T...[Objective]The aim was to select tartary buckwheat materials with high protein content.[Method]Used 35 kinds of tartary buckwheat as experimental material,and the protein content of seeds had been determined.[Result]The protein content of 35 kinds of tartary buckwheat will change in the range of 23.65-193.28 mg/g with an average of 111.85 mg/g.There was difference among different origins of tartary buckwheat.The seeds from Hezhang in Guizhou and Sichuan had highest protein content,while the seeds from Nayong had lowest protein content.[Conclusion]The study had provided theoretical basis for the further study on the genetic and variation law of protein content in different tartary buckwheat resources.展开更多
[Objective] The aim was to determine the difference in sucrose content of different tartary buckwheat sources,so as to screen low-sucrose buckwheat materials.[Method] The sucrose contents of 35 tartary buckwheat mater...[Objective] The aim was to determine the difference in sucrose content of different tartary buckwheat sources,so as to screen low-sucrose buckwheat materials.[Method] The sucrose contents of 35 tartary buckwheat materials from different regions were measured.[Result] The sucrose content of the 35 tartary buckwheat materials varied from 0.007 7% to 0.208 9% with an average of 0.051 8%.There was some difference among the tartary buckwheat materials from different regions.The seeds from Hezhang in Guizhou had higher sucrose content,while the seeds from Nayong in Guizhou had lower sucrose content.[Conclusion] The content of sucrose was significantly low in tartary buckwheat,which could be popularized as a kind of low-sugar crop.展开更多
基金Supported by the Opening Project of Key Laboratory of Coarse Cereal Processing of Ministry of Agriculture and Rural AffairsSichuan Engineering and Technology Research Center of Coarse Cereal Industralization,Chengdu University(2022CC013)。
文摘Tartary buckwheat(Fagopyrum tataricum)is a well-known pseudocereal for its health and economic value.However,abundant antinutritional factors(ANFs)reduces its health benefits.As reported,germination can improve the nutritional profile of grains.In this study,we systematically evaluate the safety of Tartary buckwheat seeds(TB)and Tartary buckwheat sprouts(TBS)used as high active ingredients.After evaluating nutrition levels,bioactive compounds and ANFs in TBS during germinating,5^(th)-day TBS were selected as the raw material.C57BL/6J mice were gavaged daily with distilled water,TB,or TBS for 6 weeks.The physiological indices related to ANFs were determined.Results showed that the TB intake tends to generate negative effects on the gut microbiota,and organs.Additionally,upon TB intake,the Fe^(3+)content in serum,trypsin activity in pancreas and jejunum decreased,while the cytokine,IgE,and histamine levels in serum,water content in faeces,cytokine levels in liver and jejunum increased.Conversely,TBS did not induce any obvious negative effects on the above relevant indices and showed better lipid-lowering effect.Altogether,TBS are safer and more effective as a raw material to produce the functional food for long-term consumption with the intention of preventing and treating hyperlipidaemia.
基金the Sichuan Science and Technology Program(2023NSFSC0214)China Agriculture Research System(CARS-07-B-1)+1 种基金National Natural Sciences Foundation of China(Nos.3230185031771716).
文摘To mitigate the wastage of seed resources and reduce the usage of pesticides and fertilizers, seed coating agentshave gained popularity. This study employs single-factor and multi-index orthogonal experimental design methodsto investigate the seed coating formula and physical properties of Tartary buckwheat. The specific effects ofeach component on Tartary buckwheat seed germination are analyzed. The findings reveal that the seed coatingagent formulated with 1.5% polyvinyl alcohol, 0.15% sodium alginate, 0.2% op-10, 0.1% polyacrylamide, 8% colorant,3% ammonium sulfate, 1% potassium dihydrogen phosphate, and 0.15% carbendazim exhibits the mosteffective coating. It demonstrates optimal physical properties and promotes seed germination efficiently. The suspensionrate of this seed coating agent reaches 91.12%, with a mere 2.13% coating shedding rate and 2.5% coatingseed rot rate. Furthermore, it achieves a germination percentage of 99.17%, which is 20.84% higher than the lowestgroup. The germination potential and index are also significantly higher than the lowest group, with anincrease of 20.84% and 26.56%, respectively. Additionally, the vitality index is 553.08, a 15.75% increase comparedto the lowest group. The application of seed coating agents helps reduce seed resource loss, increase plant numbers,and ultimately enhance agricultural yields. This finding holds practical significance in agriculturalproduction.
基金Science&Technology Department of Sichuan Province(2022YFQ0041,2022NSFSC1725,2023NSFSC0214)China Agriculture Research System(CARS-07-B-1)+2 种基金The National Natural Science Foundation of China(32160428)Innovative Training Program for College Students(202311079040,S202311079112,CDUCX2023550)Undergraduate Education and Teaching Reform Project of Chengdu University(cdjgb2022186).
文摘Aluminum (Al) toxicity is a considerable factor limiting crop yield and biomass in acidic soil. Tartary buckwheatgrowing in acidic soil may suffer from Al poisoning. Here, we investigated the influence of Al stress on the growthof tartary buckwheat seedling roots, and the alleviation of Al stress by silicon (Si), as has been demonstrated inmany crops. Under Al stress, root growth (total root length, primary root length, root tips, root surface area, androot volume) was significantly inhibited, and Al and malondialdehyde (MDA) accumulated in the root tips. At thesame time, catalase (CAT) and ascorbate peroxidase activities, polyphenols, flavonoids, and 1,1-diphenyl-2-picrylhydrazyl(DPPH) and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS) free-radical scavenging abilitywere significantly decreased. After the application of Si, root growth, Al accumulation, and oxidative damage wereimproved. Compared to Al-treated seedlings, the contents of ·O2− and MDA decreased by 29.39% and 25.22%,respectively. This was associated with Si-induced increases in peroxidase and CAT enzyme activity, flavonoidcompounds, and free-radical scavenging (DPPH and ABTS). The application of Si therefore has positive effectson Al toxicity in tartary buckwheat roots by reducing Al accumulation in the roots and maintaining oxidationhomeostasis.
文摘Diabetes is one of the most difficult chronic diseases to cure in the world,which seriously affects people’s health and quality of life.Flavonoids in buckwheat can regulate blood glucose levels by inhibitingα-amylase activity.Therefore,sweet buckwheat produced in Inner Mongolia was used as the research object,and buckwheat fl avonoids were extracted by ultrasonic-assisted extraction method.Total fl avonoids content was determined by ultraviolet-visible spectrophotometry.With acarbose as the positive control,the inhibition test ofα-amylase was carried out by DNS colorimetry to study the inhibition behavior of fl avonoids onα-amylase activity.The results showed that the extraction process of flavonoids was stable and reliable,and the established method for the determination of flavonoids was simple,accurate and reproducible.The total flavonoids content of buckwheat samples was 2.706 mg/g,buckwheat total fl avonoids extraction solution had an inhibitory eff ect onα-amylase,and its median inhibition concentration(IC_(50))was 38.53 mg/mL.The results of this experiment provide a technical reference for the development and utilization of fl avonoids in Inner Mongolia sweet buckwheat,and provide a theoretical reference for the development and application of flavonoid-rich hypoglycemic food.
基金Supported by Special Project of Guizhou Animal and Plant Breeding(QianNongYuZhuanZi[2010]023)Natural Science Foundation of China(31060207)Key Project of Guizhou Agriculture(QianKeHe NY Zi[2010]3094)~~
文摘[Objective]The study aimed to carry out RAPD analysis of genetic diversity of common and tartary buckwheat varieties.[Method] The genetic diversity of 19 common and tartary buckwheat varieties including the tested varieties in Guizhou region during 1999-2010 and their parents were studied using 7 primers by means of random amplified polymorphic DNA（RAPD）.[Result]A total of 149 DNA bands were obtained.In which,141 bands were polymorphic,accounting for 94.89%.Polymorphism analysis and cluster analysis showed that all varieties had their own special bands different from each other.The varieties native to Weining were close to each other,and other common buckwheat varieties were obviously different from each other.The interspecific genetic variation was the greatest;the intraspecific genetic variation of common buckwheat varieties was greater than that of tartary buckwheat varieties.[Conclusion]The RAPD fingerprints of the 19 buckwheat varieties were established in present study.
基金Supported by Science and Technology Foundation in Guizhou[Guizhou He J(2009)No.2108]Key Scientific Research Project for Students in Guizhou Normal University(2010),Scientific Research Fund for PhD in Guizhou Normal University(2008)~~
文摘[Objective] This study aimed to finding out the difference in glucose content of tartary buckwheat seeds from different origins.[Method] Taking 35 kinds of tartary buckwheat from different origins as the test materials,the glucose content of seeds had been determined.[Result] The variation range of glucose content in 35 kinds of tartary buckwheat was from 0.055 6% to 0.840 2% with an average of 0.321 7%.There was a difference in glucose content of tartary buckwheat from different origins.The seeds T324 from Weining in Guizhou had the highest glucose content,while the seeds T398 also from Weining in Guizhou had the lowest glucose content.[Conclusion] This study provides a reference for the genetic variation law of glucose content in different tartary buckwheat resources.
基金Supported by Science and Technology Foundation of Guizhou Province[Qiankehe-J(2009)No.2108)]Guizhou Normal University Fund for Doctor(2008)~~
文摘[Objective]The aim was to select tartary buckwheat materials with high protein content.[Method]Used 35 kinds of tartary buckwheat as experimental material,and the protein content of seeds had been determined.[Result]The protein content of 35 kinds of tartary buckwheat will change in the range of 23.65-193.28 mg/g with an average of 111.85 mg/g.There was difference among different origins of tartary buckwheat.The seeds from Hezhang in Guizhou and Sichuan had highest protein content,while the seeds from Nayong had lowest protein content.[Conclusion]The study had provided theoretical basis for the further study on the genetic and variation law of protein content in different tartary buckwheat resources.
基金Supported by the Science and Technology Foundation of Guizhou Province[J(2009)2108]Agricultural Research Project of Guizhou Province[NY(2010)3094]Special Program of Plant and Animal Breeding of Guizhou Province[No.(2010)023]~~
文摘[Objective] The aim was to determine the difference in sucrose content of different tartary buckwheat sources,so as to screen low-sucrose buckwheat materials.[Method] The sucrose contents of 35 tartary buckwheat materials from different regions were measured.[Result] The sucrose content of the 35 tartary buckwheat materials varied from 0.007 7% to 0.208 9% with an average of 0.051 8%.There was some difference among the tartary buckwheat materials from different regions.The seeds from Hezhang in Guizhou had higher sucrose content,while the seeds from Nayong in Guizhou had lower sucrose content.[Conclusion] The content of sucrose was significantly low in tartary buckwheat,which could be popularized as a kind of low-sugar crop.
