Bulked Segregant Analysis to Detect QTL Related to Heat Tolerance in Rice(Oryza sativa L.) Using SSR Markers

The study was undertaken to assess the genetic effect of quantitative trait loci(QTLs) conferring heat tolerance at flowering stage in rice.A population consisting of 279 F2 individuals from the cross between 996,a heat tolerant cultivar and 4628,a heat-sensitive cultivar,was analyzed for their segregation pattern of the difference of seed set rate under optimal temperature condition and high temperature condition.The difference of seed set rate under optimal temperature condition and high temperature condition showed normal distribution,indicating the polygenic control over the trait.To identify main effect of QTL for heat tolerance,the parents were surveyed with 200 primer pairs of simple sequence repeats(SSR).The parental survey revealed 30% polymorphism between parents.In order to detect the main QTL association with heat tolerance,a strategy of combining the DNA pooling from selected segregants and genotyping was adopted.The association of putative markers identified based on DNA pooling from selected segregants was established by single marker analysis(SMA).The results of SMA revealed that SSR markers,RM3735 on chromosome 4 and RM3586 on chromosome 3 showed significant association with heat tolerance respectively,accounted for 17 and 3% of the total variation respectively.The heat tolerance during flowering stage in rice was controlled by multiple gene.The SSR markers,RM3735 on chromosome 4 and RM3586 on chromosome 3 showed significant association with heat tolerance respectively,accounted for 17 and 3% of the total variation respectively.The two genetic loci,especially for RM3735 on chromosome 4,can be used in marker-assistant-selected method in heat tolerance breeding in rice.

水稻抽穗扬花期耐热性的QTL分析

培育高产稳产、对环境钝感的优质杂交稻新组合已成为当前水稻育种的重要目标;近年高温造成水稻结实率显著下降而导致产量的严重损失,水稻耐热性的改良也日益迫切。本研究用一个籼粳交重组自交系群体构建遗传连锁图谱,图谱拟合157个SSR标记位点,覆盖水稻基因组2352.62cM,标记间平均遗传距离14.89cM。以常温和高温结实率的差值作为耐热指数,采用复合区间作图法,对水稻抽穗扬花期的耐热性进行了QTL分析,在第2、3、5连锁群上检测到3个新的耐热QTL。贡献率为:6.59%、10.72%和10.7%,联合贡献率为28.01%。