An 8 years old Murrah buffalo in its 5^(th) party was presented with the history of 9 month of gestation and sudden bilateral abdominal distension since last 25 days.Animal was 7 anorectic with pale mucus membrane and...An 8 years old Murrah buffalo in its 5^(th) party was presented with the history of 9 month of gestation and sudden bilateral abdominal distension since last 25 days.Animal was 7 anorectic with pale mucus membrane and had staggering gait.Per vaginum examination revealed closed cervix with intact cervical seal.By transrectal palpation only the fluid in the uterus could be palpable.Based on history and clinical examination findings,it was diagnosed as hydroallantois.Parturition was induced using cloprostenol and dexamethasone.Beside this an intracervical injection of closprostenol was also given on 6 o’clock and 12 o’clock position(250μg each)of external os to hasten the cervical dilation.A dead bulldog calf was removed with manual traction after 26 hours of initial treatment.It was concluded that the cloprostenol and dexamethasone are effective for the induction of parturition in animals suffering from hydroallantois provided fluid from the allantoic sac is removed slowly.展开更多
为了探究移植脐带间充质干细胞(UC-MSCs)对荷斯坦公犊牛日增重和血清中激素水平的影响,试验选取1月龄体重相近的荷斯坦公犊牛28头,随机分为对照组和试验组;采集健康的荷斯坦新生胎牛的脐带组织,通过胰酶消化筛选法体外分离、纯化及培养...为了探究移植脐带间充质干细胞(UC-MSCs)对荷斯坦公犊牛日增重和血清中激素水平的影响,试验选取1月龄体重相近的荷斯坦公犊牛28头,随机分为对照组和试验组;采集健康的荷斯坦新生胎牛的脐带组织,通过胰酶消化筛选法体外分离、纯化及培养牛脐带间充质干细胞(UCMSCs),试验组公犊牛注射干细胞3×105个/kg(稀释至8 m L颈静脉注射),对照组注射等量生理盐水,试验期为155 d。结果表明:注射UC-MSCs后,4,5,6月龄时,试验组公犊牛平均体重显著高于对照组(P<0.05)。4月龄时,试验组公犊牛平均日增重显著高于对照组(P<0.05);6月龄时,试验组公犊牛平均日增重极显著高于对照组(P<0.01)。4月龄时,试验组公犊牛血清中生长抑素(SS)水平显著低于对照组(P<0.05);6月龄时,试验组公犊牛血清中甲状腺素(T4)、三碘甲腺原氨酸(T3)水平差异极显著(P<0.01)。说明移植UC-MSCs能显著增加荷斯坦公犊牛的体增重,降低血清中生长激素(GH)、SS、瘦素(LEP)的水平,提高血清中T4、T3水平,而血清中这些激素水平与公犊牛的生长发育密切相关,进而影响其生长性能。展开更多
文摘An 8 years old Murrah buffalo in its 5^(th) party was presented with the history of 9 month of gestation and sudden bilateral abdominal distension since last 25 days.Animal was 7 anorectic with pale mucus membrane and had staggering gait.Per vaginum examination revealed closed cervix with intact cervical seal.By transrectal palpation only the fluid in the uterus could be palpable.Based on history and clinical examination findings,it was diagnosed as hydroallantois.Parturition was induced using cloprostenol and dexamethasone.Beside this an intracervical injection of closprostenol was also given on 6 o’clock and 12 o’clock position(250μg each)of external os to hasten the cervical dilation.A dead bulldog calf was removed with manual traction after 26 hours of initial treatment.It was concluded that the cloprostenol and dexamethasone are effective for the induction of parturition in animals suffering from hydroallantois provided fluid from the allantoic sac is removed slowly.
文摘为了探究移植脐带间充质干细胞(UC-MSCs)对荷斯坦公犊牛日增重和血清中激素水平的影响,试验选取1月龄体重相近的荷斯坦公犊牛28头,随机分为对照组和试验组;采集健康的荷斯坦新生胎牛的脐带组织,通过胰酶消化筛选法体外分离、纯化及培养牛脐带间充质干细胞(UCMSCs),试验组公犊牛注射干细胞3×105个/kg(稀释至8 m L颈静脉注射),对照组注射等量生理盐水,试验期为155 d。结果表明:注射UC-MSCs后,4,5,6月龄时,试验组公犊牛平均体重显著高于对照组(P<0.05)。4月龄时,试验组公犊牛平均日增重显著高于对照组(P<0.05);6月龄时,试验组公犊牛平均日增重极显著高于对照组(P<0.01)。4月龄时,试验组公犊牛血清中生长抑素(SS)水平显著低于对照组(P<0.05);6月龄时,试验组公犊牛血清中甲状腺素(T4)、三碘甲腺原氨酸(T3)水平差异极显著(P<0.01)。说明移植UC-MSCs能显著增加荷斯坦公犊牛的体增重,降低血清中生长激素(GH)、SS、瘦素(LEP)的水平,提高血清中T4、T3水平,而血清中这些激素水平与公犊牛的生长发育密切相关,进而影响其生长性能。