AIM To explore the kinetic changes in plasma D (-)lactate and lipopolysaccharide ( LPS ) levels, and investigate whether D (-)-lactate could be used as a marker of intestinal injury in rats following gut ischemia/repe...AIM To explore the kinetic changes in plasma D (-)lactate and lipopolysaccharide ( LPS ) levels, and investigate whether D (-)-lactate could be used as a marker of intestinal injury in rats following gut ischemia/reperfusion, burn, and acute necrotizing pancreatitis (ANP).METHODS Three models were developed in rats:gut ischemia/reperfusion obtained by one hour of superior severe burn injury created by 30% of total body surface induced by continuous inverse infusion of sodium taurocholate and trypsin into main pancreatic duct.Plasma levels of D( - )-lactate in systemic circulation and LPS in portal circulation were measured by enzymaticspectrophotometric method and limulus amebocyte lysate (LAL) test kit, respectively. Tissue samples of intestine were taken for histological analysis.RESULTS One hour gut ischemia followed by reperfusion injuries resulted in a significant elevation in plasma D( - )lactate and LPS levels, and there was a significant correlation between the plasma D( - )-lactate and LPS (r =0.719, P＜0.05). The plasma concentrations of D(-)lactate and LPS increased significantly at 6 h postburn,and there was also a remarkable correlation between them (r = 0.877, P ＜ 0.01). D ( - )-lactate and LPS levels elevated significantly at 2 h after ANP, with a similar significant correlation between the two levels (r-0.798,P ＜ 0.01 ). The desquamation of intestine villi and infiltration of inflammatory cells in the lamina propria were observed in all groups.CONCLUSION The changes of plasma D( - )-lactate levels in systemic blood paralleled with LPS levels in the portal vein blood. The measurement of plasma D (-)-lactate level may be a useful marker to assess the intestinal injury and to monitor an increase of intestinal permeability and endotoxemia following severe injuries in early stage.展开更多
INTRODUCTION The blood irrigate flow obstruction,especially thegastrointestinal(GI)ischemia,is the main factorof the damage to the digestive tract caused byserious burns.The effect of GI ischemia on thewhole body is e...INTRODUCTION The blood irrigate flow obstruction,especially thegastrointestinal(GI)ischemia,is the main factorof the damage to the digestive tract caused byserious burns.The effect of GI ischemia on thewhole body is extensive and profound,which notonly causes the increase of intestinal permeabilityand the movement of bacteria and toxin in theintestinal cavity,but releases a large quantity ofinflammatory media.Neuroendocrine element展开更多
Abstract ：Aim To assess therapeutic effect and mechanism of Oxytropis falcata Bung gel compound （OFBGC） in rats. Methods Wistar rats were divided into： model group, vehicle group, administration groups（low-dose, ...Abstract ：Aim To assess therapeutic effect and mechanism of Oxytropis falcata Bung gel compound （OFBGC） in rats. Methods Wistar rats were divided into： model group, vehicle group, administration groups（low-dose, middle- dose and high-dose group）, positive control and normal control. Deep Ⅱ° burn rat model was used and the animals were treated （2/d）, respectively. In order to assess the wound healing effect, HE staining was conducted. Immuno- histochemical detection for EGF, VEGF and CD34 were performed. ELISA detection for EGF, VEGF, p38 and IL-1β were carried out in serum. Results According to HE staining, administration groups could reduce conspicuously wound scabby time, accelerate wound healing, promote the hyperplasia of sebaceous glands and hair follicles, de- crease the inflammatory cells and relieve swelling in different degree. The expression of EGF and CD34 in burning tissue increased remarkably in 7th day from immunohistochemical assessment. Serum EGF expression went up con- spicuously （P 〈 0. 01 ）, but p38 and IL-1β levels were dramatically lower compared to the model groups. Conclusions OFBGC could promote the wound healing in deep Ⅱ° burn rats. The mechanism may be related, at least in part, to increasing the biosynthesis and release of EGF and CD34 in burning tissue, decreasing serum p38 and IL-1β levels.展开更多
In this case report, we describe our experience of managing a patient with chemical burns caused by hot chromic acid that covered over 60% of the patient＇s body. The patient developed anuria 48 hours after injury. Ea...In this case report, we describe our experience of managing a patient with chemical burns caused by hot chromic acid that covered over 60% of the patient＇s body. The patient developed anuria 48 hours after injury. Early excision of burn eschars and hemodialysis were carried out. The patient survived after a series of comprehensive treatments, including allografting and autografting. In patients burned by hot chromic acid, excision of affected skin down to the muscle fascia should be carried out as soon as possible after injury. Dialysis to remove circulating chromium in the first 24 hours after injury is also recommended.展开更多
基金the Fund for National Outstanding Young Researchers of China
文摘AIM To explore the kinetic changes in plasma D (-)lactate and lipopolysaccharide ( LPS ) levels, and investigate whether D (-)-lactate could be used as a marker of intestinal injury in rats following gut ischemia/reperfusion, burn, and acute necrotizing pancreatitis (ANP).METHODS Three models were developed in rats:gut ischemia/reperfusion obtained by one hour of superior severe burn injury created by 30% of total body surface induced by continuous inverse infusion of sodium taurocholate and trypsin into main pancreatic duct.Plasma levels of D( - )-lactate in systemic circulation and LPS in portal circulation were measured by enzymaticspectrophotometric method and limulus amebocyte lysate (LAL) test kit, respectively. Tissue samples of intestine were taken for histological analysis.RESULTS One hour gut ischemia followed by reperfusion injuries resulted in a significant elevation in plasma D( - )lactate and LPS levels, and there was a significant correlation between the plasma D( - )-lactate and LPS (r =0.719, P＜0.05). The plasma concentrations of D(-)lactate and LPS increased significantly at 6 h postburn,and there was also a remarkable correlation between them (r = 0.877, P ＜ 0.01). D ( - )-lactate and LPS levels elevated significantly at 2 h after ANP, with a similar significant correlation between the two levels (r-0.798,P ＜ 0.01 ). The desquamation of intestine villi and infiltration of inflammatory cells in the lamina propria were observed in all groups.CONCLUSION The changes of plasma D( - )-lactate levels in systemic blood paralleled with LPS levels in the portal vein blood. The measurement of plasma D (-)-lactate level may be a useful marker to assess the intestinal injury and to monitor an increase of intestinal permeability and endotoxemia following severe injuries in early stage.
文摘INTRODUCTION The blood irrigate flow obstruction,especially thegastrointestinal(GI)ischemia,is the main factorof the damage to the digestive tract caused byserious burns.The effect of GI ischemia on thewhole body is extensive and profound,which notonly causes the increase of intestinal permeabilityand the movement of bacteria and toxin in theintestinal cavity,but releases a large quantity ofinflammatory media.Neuroendocrine element
文摘Abstract ：Aim To assess therapeutic effect and mechanism of Oxytropis falcata Bung gel compound （OFBGC） in rats. Methods Wistar rats were divided into： model group, vehicle group, administration groups（low-dose, middle- dose and high-dose group）, positive control and normal control. Deep Ⅱ° burn rat model was used and the animals were treated （2/d）, respectively. In order to assess the wound healing effect, HE staining was conducted. Immuno- histochemical detection for EGF, VEGF and CD34 were performed. ELISA detection for EGF, VEGF, p38 and IL-1β were carried out in serum. Results According to HE staining, administration groups could reduce conspicuously wound scabby time, accelerate wound healing, promote the hyperplasia of sebaceous glands and hair follicles, de- crease the inflammatory cells and relieve swelling in different degree. The expression of EGF and CD34 in burning tissue increased remarkably in 7th day from immunohistochemical assessment. Serum EGF expression went up con- spicuously （P 〈 0. 01 ）, but p38 and IL-1β levels were dramatically lower compared to the model groups. Conclusions OFBGC could promote the wound healing in deep Ⅱ° burn rats. The mechanism may be related, at least in part, to increasing the biosynthesis and release of EGF and CD34 in burning tissue, decreasing serum p38 and IL-1β levels.
文摘In this case report, we describe our experience of managing a patient with chemical burns caused by hot chromic acid that covered over 60% of the patient＇s body. The patient developed anuria 48 hours after injury. Early excision of burn eschars and hemodialysis were carried out. The patient survived after a series of comprehensive treatments, including allografting and autografting. In patients burned by hot chromic acid, excision of affected skin down to the muscle fascia should be carried out as soon as possible after injury. Dialysis to remove circulating chromium in the first 24 hours after injury is also recommended.
