To acquire more understanding about the myocardial cell membrane structural damageduring severe burns on the molecular level. Methods: In the in vivo burn model established in rats, alterations of the anion sites at h...To acquire more understanding about the myocardial cell membrane structural damageduring severe burns on the molecular level. Methods: In the in vivo burn model established in rats, alterations of the anion sites at hasal lamina of the myocardial cells was assessed with a PEI cationic probe. The membrane permeability of the myocardial cells was observed using colloidal lanthanum as a marker and the myocardial water content measured. Results: The anion sites of hasal lamina of the myocardial cells showed regular linear lattice arrangement (40 nm- 80nm). The PEI granules of the hasal lamina were decreased at the lst h and decreased significantly at the 6th h after burns. The lanthanum granules entered into the myocardial cells at the lst h and the myocardial water content was significantly increased (P<0. 01 ) at the 3rd h after burns. Conclusion: The damage of the hasal lamina and decrease of the anion sites on the myocardial cells might be a contributive factor causing the increase of membrane permeability, tissue edema and membrane protective barrier dysfunction after severe burns.展开更多
The effects of toxic substance D1,extracted from burn eschars,on the hepatic mitochondrial respiratory function and its damages to the respirtatory chain were observed in the rat.It was found that the rate of stste 3r...The effects of toxic substance D1,extracted from burn eschars,on the hepatic mitochondrial respiratory function and its damages to the respirtatory chain were observed in the rat.It was found that the rate of stste 3respiration was inhibited and that of state 4 respiration stimulated by D1.There was an obvious dosage-effect relationship between D1 dosage and the rate of oxygen consumption, that is,the changes of the rate was slow in the range of low dosage and fast in the range of high dosage. The point of change was at 1 mg/ml of D1 concentration.The ratio of FCCP-stimulated respiration rate/state 4 respiration rate was decreased when D1 dosage was increased.The results of experiments were similar whether glutamate+malate or succinate were used as the substrate. The point of change was also at 1 mg/ml of D1 concentration. The decrease of FCCP-stimulated rate/state 4rate implied that the respiratory chain was damaged.But complex Ⅳ-the terminal segment of the chain-remained intact. Thus, the site of damage must be somewhere in complexesⅠ￣Ⅲ and/or complexes Ⅱ￣Ⅲ. Since NADH dehydrogenase and succinate dehydrogenase-the main enzymes in complexesⅠand Ⅱ-were not affected by D1 but NADH: cytochrome c reductase and succinate:cytochrome c reductase markedly inhibited, the site of damage was most likely located in complex Ⅲ-the bc1 complex.展开更多
Full thickness skin grafts and composite grafts are a workhorse in reconstructive surgery of nose and ear defects whether they are originated from trauma, cancer surgery or burns. The reliability of these grafts has b...Full thickness skin grafts and composite grafts are a workhorse in reconstructive surgery of nose and ear defects whether they are originated from trauma, cancer surgery or burns. The reliability of these grafts has been proved with an established clinical use and morbidity to the donor site is minimal if harvesting and donor site selection if appropriate. Use of double or multiple grafts to reconstruct a complex defect of the nose has not been described and our report is meaningful for the fact that it describes a further use of the surgical concept of grafting.展开更多
文摘To acquire more understanding about the myocardial cell membrane structural damageduring severe burns on the molecular level. Methods: In the in vivo burn model established in rats, alterations of the anion sites at hasal lamina of the myocardial cells was assessed with a PEI cationic probe. The membrane permeability of the myocardial cells was observed using colloidal lanthanum as a marker and the myocardial water content measured. Results: The anion sites of hasal lamina of the myocardial cells showed regular linear lattice arrangement (40 nm- 80nm). The PEI granules of the hasal lamina were decreased at the lst h and decreased significantly at the 6th h after burns. The lanthanum granules entered into the myocardial cells at the lst h and the myocardial water content was significantly increased (P<0. 01 ) at the 3rd h after burns. Conclusion: The damage of the hasal lamina and decrease of the anion sites on the myocardial cells might be a contributive factor causing the increase of membrane permeability, tissue edema and membrane protective barrier dysfunction after severe burns.
文摘The effects of toxic substance D1,extracted from burn eschars,on the hepatic mitochondrial respiratory function and its damages to the respirtatory chain were observed in the rat.It was found that the rate of stste 3respiration was inhibited and that of state 4 respiration stimulated by D1.There was an obvious dosage-effect relationship between D1 dosage and the rate of oxygen consumption, that is,the changes of the rate was slow in the range of low dosage and fast in the range of high dosage. The point of change was at 1 mg/ml of D1 concentration.The ratio of FCCP-stimulated respiration rate/state 4 respiration rate was decreased when D1 dosage was increased.The results of experiments were similar whether glutamate+malate or succinate were used as the substrate. The point of change was also at 1 mg/ml of D1 concentration. The decrease of FCCP-stimulated rate/state 4rate implied that the respiratory chain was damaged.But complex Ⅳ-the terminal segment of the chain-remained intact. Thus, the site of damage must be somewhere in complexesⅠ￣Ⅲ and/or complexes Ⅱ￣Ⅲ. Since NADH dehydrogenase and succinate dehydrogenase-the main enzymes in complexesⅠand Ⅱ-were not affected by D1 but NADH: cytochrome c reductase and succinate:cytochrome c reductase markedly inhibited, the site of damage was most likely located in complex Ⅲ-the bc1 complex.
文摘Full thickness skin grafts and composite grafts are a workhorse in reconstructive surgery of nose and ear defects whether they are originated from trauma, cancer surgery or burns. The reliability of these grafts has been proved with an established clinical use and morbidity to the donor site is minimal if harvesting and donor site selection if appropriate. Use of double or multiple grafts to reconstruct a complex defect of the nose has not been described and our report is meaningful for the fact that it describes a further use of the surgical concept of grafting.
