PCR-DGGE Analysis of Bacterial Communities Structure in Babylonia areolata Culture Systems of The Subtidal Zone and The Pond Mulched Plastic Film and Sand in Bottom

To know the bacterial communities structure in Babylonia areolata culture systems and to research and optimize the management pattem of Babylonia areola-ta culture systems of the pond mulched plastic film and sand in bottom, the bacte- rial communities in Babylonia areolata culture systems of the sub-tidal zone and the pond mulched plastic film and sand in bottom were analyzed at molecular level by adopting the denaturing gradient gel electrophoresis （DGGE）. The results indicated that the dominant bacterial communities in Babylonia areolata culture systems of the sub-tidal zone and the pond mulched plastic film and sand in bottom, which were built on the basis of the seawater in East-island of Zhanjiang, included Proteobac- teda Chloroflexi, Cyanobacteria and Actinobacteria. The dominant bacterial groups in the above pond culture system were Garnmaproteobacteria, Alphaproteobacteria, Deltaprotecbacteda, Epsilonproteobacteda, Anaerolineae, Cyanobacteria and Acti- nobacteda. The dominant bacterial communities in the subtidal zone culture system were Gammaprotecbacteda, Alphaproteobacteria, Deltaproteobacteria, Anaerolineae and Cyanobacteda, and there were less Epsilonproteobacteria and Actinobacteria in the culture system. The higher diversity was detected in the above two culture sys- tems. The results of unweighted pair group method with arithmetic average （UPG- MA） showed that the bacterial communities of the sediment samples S1 and S2 in the above two culture systems were a cluster, the similarity of bacterial communities was 54.5%. The bacterial communities of seawater samples S3 and S4 in the above culture systems were in clusters, and the similarity of the bacterial communi- ties was 84.0%. The results showed that the microorganism ecological level in the Babylonia areolata culture systems of the pond mulched plastic film and sand in bottom could be similar to the sub-tidal zone culture systems through changing the pond seawater and monitoring the microbial population.

Factors Influencing High Temperature Thermo Plasticity of BNbRE Steel at the Third Brittle Zone

In view of fine cracks occurred on the surface of BNbRE continuous casting slabs for heavy rail in the practical production, the effect of two factors, i.e. deformation temperature, deformation velocity, on thermo plasticity of BNbRE steel at the third brittle zone was quantitatively investigated on GLEEBLE-1500D thermal-mechanical simulator after measuring the RA-T curve of BNbRE steel. The results provide experimental data for optimizing the technology of continuous casting secondary cooling zone and avoiding the occurrence of fine surface cracks.

Plastic zone distribution laws and its types of surrounding rock in large-span roadway

In order to study the distribution laws and types of plastic zone of surrounding rock in large-span roadway, we analyzed the distribution laws with different spans and lateral pressures using FLAC3D numerical calcu- lation software. Based on the roadway support difficulty and distribution laws of the plastic zone of sur- rounding rock, we defined the large-span roadway and classified the types of large-span rectangular roadways. As a result, the distribution laws of the plastic zone on surrounding rock in a rectangular roadway with different spans and lateral pressures were obtained. The results show that the area of the plastic zone on surrounding rock increased with the increase of the spans and lateral pressures, and the plastic zone was symmetrical to the center line of roadway. At λ=0.5, 1.0, 1.5, and 2.0, the plastic zone presented ＂addle- shape＂ distribution, ＂ellipse＂ distribution, ＂thin and high＂ distribution and ＂inverse trapezium＂ distribu- tion, respectively. In addition, we classified the roadways into four types according to the different lateral pressures and spans, including small-span, moderate-span, large-span and extreme-large-span roadways.

Dilation angle variations in plastic zone around tunnels in rocks-constant or variable dilation parameter

Dilation angle is a significant parameter needed for numerical simulation of tunnels.Even though dilation parameter is physically variable and dependent on confinement and experienced shear plastic strain based on the existing dilation models,numerical simulations of tunnels and underground openings with constant dilation parameter usually lead to satisfactory results in practical use.This study aims to find out why constant dilation angle is enough under practical conditions to simulate numerically tunnels and underground excavations in spite of the fact that dilation angle is variable in laboratory and experimental scale.With this aim,this work studies how mobilized dilation angle varies in a plastic zone surrounding a tunnel.For the circular tunnel under uniform in situ stress field,the stepwise finite difference approximation analytical solution considering strain softening rock mass behavior with mobilized dilation angle was used to study how mobilized dilation angle varies in plastic zone around tunnel under very different conditions.In practical conditions determined in this study,dilative behavior of all over the plastic zone around the tunnel can be approximated to constant dilation angle in the middle region of the plastic zone.Moreover,the plastic zone displacements for mobilized and constant dilation angle models are compared with each other.Further investigation under more general non-uniform in situ stress conditions and non-circular tunnels is performed by using the commercial finite difference software to numerically simulate the Mine-by experimental tunnel of AECL(Atomic Energy of Canada Limited)and the arched tunnel.Although the Mine-by and arched tunnels were numerically simulated based on the mobilized dilation angle model,the variability associated with dilation angle around the simulated Mine-by and arched tunnels is insignificant,and dilation angle is approximately constant in the plastic zone.

Rules of distribution in a plastic zone of rocks surrounding a roadway affected by tectonic stress

In order to study the rules of distribution in a plastic zone of rocks, surrounding a roadway, affected by tectonic stress, we first analyzed the mechanics of a roadway affected by tectonic stress and derived a theoretical formula for the plastic zone of rocks surrounding a roadway. We also analyzed the distribution characteristics of the plastic zone under different levels of tectonic stress, vertical pressure, cohesion and friction angle of the surrounding rock. Secondly, we used numerical simulation to analyze the range and shape features of the plastic zone of rocks surrounding the roadway, given different tectonic stress levels. Finally we used a rock drilling detector to carry out field measurements on the broken state of rock surrounding the roadway at the –700 substation and channels in the Xinzhuang mine of the Shenhuo mining area. Given the measured ground stress, we analyzed the relationship between tectonic stress and the distribution of this plastic zone. Our results show that the range of the plastic zone at the top and bottom of the roadway increases with an increase in tectonic stress and this increase is especially obvious at the roadway corner.

Prediction of plastic zone size around circular tunnels in non-hydrostatic stress field

This paper discusses the calculation of plastic zone properties around circular tunnels to rock-masses that satisfy the Hoek–Brown failure criterion in non-hydrostatic condition,and reviews the calculation of plastic zone and displacement,and the basis of the convergence–confinement method in hydrostatic condition.A two-dimensional numerical simulation model was developed to gain understanding of the plastic zone shape.Plastic zone radius in any angles around the tunnel is analyzed and measured,using different values of overburden(four states)and stress ratio(nine states).Plastic zone radius equations were obtained from fitting curve to data which are dependent on the values of stress ratio,angle and plastic zone radius in hydrostatic condition.Finally validation of this equation indicate that results predict the real plastic zone radius appropriately.

PLASTIC ZONE OF SEMI-INFINITE CRACK IN PLANAR KAGOME AND TRIANGULAR LATTICES

The fracture investigations of the planar lattices made of ductile cell walls are currently limited to bending-dominated hexagonal honeycomb. In this paper, the plastic zones of stretching-dominated lattices, including Kagome and triangular lattices, are estimated by analyzing their effective yield loci. The normalized in-plane yield loci of these two lattices are almost identical convex curves enclosed by 4 straight lines, which is almost independent of the relative density but is highly sensitive to the principal stress directions. Therefore, the plastic zones around the crack tip of Kagome and triangular are estimated to be quite different to those of the continuum solid and also hexagonal lattice. The plastic zones predictions by convex yield surfaces of both lattices are validated by FE calculations, although the shear lag region caused by non-local bending effect in the Kagome lattice enlarges the plastic zone in cases of small ratio of rp/l.

INTERACTION BETWEEN A SCREW DISLOCATION AND A PLASTIC ZONE OF AN ARBITRARY SHAPE

A plastic deformation zone near a screw dislocation is treated as an equivalent transformation inclusion by means of the Eshelby inclusion theory. A closed form solution for determining the interaction between a screw dislocation and a plastically deformed zone of an arbitrary shape is obtained by using the solution between a dislocation and an equivalent transformation inclusion.

Elastic-plastic analysis of antiplane crack near crack surface region

The elastic-plastic stress distribution and the elastic-plastic boundary con- figuration near a crack surface region are significant but hard to obtain by means of the conventional analysis. A crack line analysis method is developed in this paper by consid- ering the crack surface as an extension of the crack line. The stresses in the plastic zone, the length, and the unit normal vector of the elastic-plastic boundary near a crack surface region are obtained for an antiplane crack in an elastic-perfectly plastic solid. The usual small scale yielding assumptions are not needed in the analysis.

General forms of elastic-plastic matching equations for mode-Ⅲ cracks near crack line

Crack line analysis is an effective way to solve elastic-plastic crack problems. Application of the method does not need the traditional small-scale yielding conditions and can obtain sufficiently accurate solutions near the crack line. To address mode- Ⅲ crack problems under the perfect elastic-plastic condition, matching procedures of the crack line analysis method axe summarized and refined to give general forms and formulation steps of plastic field, elastic-plastic boundary, and elastic-plastic matching equations near the crack line. The research unifies mode-III crack problems under different conditions into a problem of determining four integral constants with four matching equations. An example is given to verify correctness, conciseness, and generality of the procedure.

EXACT SOLUTIONS OF NEAR CRACK LINE FIELDS FOR MODE I CRACK UNDER PLANE STRESS CONDITION IN AN ELASTIC－PERFECTLY PLASTIC SOLID

The near crack line analysis method has been used in the present paper,The classical small scale yielding conditions have been completely abandoned in the analyses and one inappropriate matching condition used to be used at the elasticplastic boundary has been corrected.The reasonable solution of the plastic stresses near the crack line region has been established.By matching the plastic stresses with the exact elastic stresses at the elastic-plastic boundary,the plastic stresses the length of the plastic zone and the unit normal vector of the elastic-plastic boundary near the crock line region have been obtained for a mode I crack under uniaxial tension,as well as a mode I crack under biaxial tension,which shows that for both conditions the plastic stress componentsσy, and σsy.he length of the plastic zone and the unit normal vector of the elastic-plastic boundary are quite the same while the plastic stress σs is different.

Comparative studies on phenotypic plasticity of two herbs,Changium smyrnioides and Anthriscus sylvestris

The endangered medicinal herb, Changium smyrnioides can only be found in deciduous forest gaps within the middle to northern subtropical broad-leaved evergreen forest zone of China. The considerable plasticity of its shoot and root structure helps it to capture light more effectively in winter and early spring, and to adapt to the soil moisture conditions in its narrow habitat. Another medicinal plant, Anthriscus sylvestris, is of similar economic importance but commonly distributed widely. In contrast to C. smyrnioides, it has low structural plasticity. It is also specialized to adapt to the moist and sunny environment, where habitat, such as the banks of creeks and rivers, is abundant.

Formation Depth of Coesite-Bearing Eclogite,Dabie UHPM Zone,China

The plastic deformation of garnet in coesite bearing eclogite, quartz eclogite and garnet amphibolite of the UHPM complex in Yingshan County in the Dabie Mountains has been studied. The stress generated by the strong tectonic movement was an important component of the total pressure that resulted in the formation of the eclogite in the Dabie UHPM zone. The three dimensional tectonic principal stresses and additional tectonic stress induced hydrostatic pressure [ p s=( σ 1+ σ 2+ σ 3)/3] are reconstructed according to the differential stress and the strain ratio ( α ) of the garnet in the minor coesite bearing eclogite of the Yingshan County. Then the gravity induced hydrostatic pressure ( p g) is calculated following the equation p minus p s, where p is estimated to be 2.8 GPa based on the quartz coesite geobarmeter. Therefore, the thickness of the rock column overlying the coesite bearing eclogite in the Ying shan County is determined ≥32 km. This estimation, significantly different from ≥100 km, the previous one obtained solely based on the weight/specific weight ratio (W/SW), offers a proper explanation for the puzzle that no tracer of the addition of mantle derived material has been found in the Dabie UHPM zone during the process of UHPM, although a number of researchers claim that this process took place at the depth of the mantle (≥100 km). It is concluded that attention should be paid to the additional tectonic stress induced hydrostatic pressure in the study of UHPM zones.

Stresses and Shear Fracture Zone of Jinshazhou Tunnel Surrounding Rock in Rich Water Region

Field evidence has shown that large-scale and unstable discontinuous planes in the rock mass surrounding tunnels in rich water region are probably generated after excavation. The tunnel surrounding rock was divided into three zones, including elastic zone, plastic damage zone and shear fracture zone for assessing the stability of the tunnel surrounding rock. By local hydrogeology, the stresses of surrounding rock of Jinshazhou circular tunnel was analyzed and the stress solutions on the elastic and plastic damage zones were obtained by applying the theories of fluid-solid coupling and elasto-plastic damage mechanics. The shear fracture zone generated by joints was studied and its range was determined by using Molar-Coulomb strength criterion. Finally, the correctness of the theoretical results was validated by comparing the scopes of shear fracture zones calculated in this paper with those from literature.

A SUPPLEMENTARY STUDY OF ANISOTROPIC PLASTIC FIELDS AT A RAPIDLY PROPAGATING PLANE－STRESS CRACK－TIP（I）

The results in Ref. [1] are not suitable for the cases of β≥2. For this reason, byusing the methods in Ref [1] and Ref [2], we derive the general expressions ofamsotropic plastic fields at a rapidly propagating plane-stress crack-tip for both thecases of β=2 and β>2 .

Elastoplastical analysis on cylinder of different moduli in tension and compression

The constitutive equation was deduced. The results were obtained from theelastic and elastoplastical analysis of the cylinder sample applied with different ratios ofload on both the inner and outer sides and different moduli ratio. The factors affecting thedevelopment of the plastic field of the cylinder with finite radius were shown. The resultsshow that the different moduli ratio is the most important factor in the development of theplastic zone. The slight fluctuation of the ratio will bring multiplied increment of the displacement, which may result in the final destruction of the engineering material.

Contact dynamics of elasto-plastic thin beams simulated via absolute nodal coordinate formulation

Under the frame of multibody dynamics, the contact dynamics of elasto-plastic spatial thin beams is numerically studied by using the spatial thin beam elements of absolute nodal coordinate formulation（ANCF）. The internal force of the elasto-plastic spatial thin beam element is derived under the assumption that the plastic strain of the beam element depends only on its longitudinal deformation.A new body-fixed local coordinate system is introduced into the spatial thin beam element of ANCF for efficient contact detection in the contact dynamics simulation. The linear isotropic hardening constitutive law is used to describe the elasto-plastic deformation of beam material, and the classical return mapping algorithm is adopted to evaluate the plastic strains. A multi-zone contact approach of thin beams previously proposed by the authors is also introduced to detect the multiple contact zones of beams accurately, and the penalty method is used to compute the normal contact force of thin beams in contact. Four numerical examples are given to demonstrate the applicability and effectiveness of the proposed elasto-plastic spatial thin beam element of ANCF for flexible multibody system dynamics.

巷道蝶形破坏强度准则低敏感性研究及工程应用

巷道围岩的塑性区形态对巷道的破坏形式及破坏程度有重要影响。为探究三向应力场下塑性区形态演化过程,基于弹性力学推导了轴向应力表达式,并依据蝶形塑性区边界方程求解思路,确定了三维强度准则下三向塑性区近似解的求解方法。通过等球应力p、等偏应力q以及不同Lode角θσ来确定围岩应力加载方案,对不同三维强度准则下的围岩塑性区形态演化规律进行深入研究,论证了蝶形破坏的准则低敏感性。基于蝶形破坏理论对羊场湾160206回风巷道的非对称变形破坏机制及控制技术进行深入分析。研究结果表明:(1)在相同p、q及不同θσ的应力加载条件下,5种强度准则下的塑性区形态均呈现圆形、类椭圆及蝶形的演化规律,且每种强度准则在相同θσ的情况下围岩的塑性区形态基本一致。(2)相同应力大小、不同应力方向的加载方案下,围岩的塑性区形态大不相同。圆巷围岩的塑性区形态很大程度上由水平侧压比决定,轴向侧压对围岩的塑性区尺寸影响较大,对塑性区形态影响较小。(3)羊场湾160206回风巷道在叠加采动影响下顶板呈现非对称大变形破坏,基于蝶形塑性区支护思路,应用非对称锚杆索+超前单元支架+钻孔卸压的协同支护技术,取得了良好的支护效果。

掏土孔应力扰动法下的塑性区范围研究

针对普通浅层掏土法下倾斜建筑回倾速度慢、沉降不均匀的问题,提出掏土孔应力扰动法。在岩石力学的弹性圈和塑性圈应力解中引入修正系数,得到掏土孔应力扰动法下的塑性区半径计算公式。在进行现场掏土孔周应力扰动试验时,采用地锚反压的形式模拟上部建筑荷载,利用长钉在掏土孔周围进行深度为50 mm的螺旋扰动,通过测量应力扰动前后的孔周土体应力并结合有限元模拟结果,得到实际塑性区范围,验证了塑性区半径公式的正确性。结果表明:当掏土孔半径为100 mm,应力扰动范围为孔径的1/2时,塑性区范围扩大30%~40%,掏土孔应力扰动法对于扩大塑性区范围、加快建筑纠倾速度具有工程应用价值。

三向非等压应力场下围岩主应力差与塑性区分布关系研究

首先,以弹性理论为基础推导出考虑巷道轴向应力影响的主应力差解析式,通过变化水平侧压比、轴向侧压比研究完全弹性条件时不同应力场下主应力差分布形态演化规律。其次,推导出考虑轴向应力作用的围岩塑性区近似解,研究不同应力环境下围岩的塑性区形态演化规律。最后,对比分析主应力差及塑性区形态特征,研究二者分布形态的对应关系,并通过数值模拟验证理论分析的正确性。研究结果表明:在弹性理论下,三向应力场条件的主应力差值分布会呈现圆形、椭圆形、蝶形3种形态的演化规律;在考虑巷道塑性破坏的情况下,主应力差值在σx、σz较小一侧形成峰值壳,在较大一侧形成卸压壳。在不同水平侧压比及轴向侧压比情况下,围岩塑性区形态会呈现圆形—椭圆形、椭圆形—蝶形、蝶形恶性扩展3个阶段的演化过程。巷道主应力差的分布形态在一定程度上可以反映塑性区形态,主应力差和塑性区在相同应力条件下的形态特征具有逐一对应关系,且二者形态在很大程度上由水平侧压比决定,轴向侧压比对塑性区形态影响较小。160206回风巷在偏转式主应力差集中区形成非对称异化特征。本文基于分析及现场破坏情况提出的联合协同支护技术在现场应用效果较好。