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NIK和IKKβ连接蛋白、C-myc和细胞周期蛋白D1在结肠癌组织中的表达及意义 被引量:13
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作者 李素艳 黄杰安 +7 位作者 谭林 覃蒙斌 刘诗权 刘宝玉 梁梦紫 徐春燕 彭鹏 李佳荃 《中国全科医学》 CAS CSCD 北大核心 2015年第24期2922-2928,共7页
目的检测结肠癌组织中NIK和IKKβ连接蛋白(NIBP)、磷酸化p65(p-p65)和C-myc、细胞周期蛋白D1(cyclin D1)的表达,并探讨其意义。方法收集广西医科大学第一附属医院结直肠外科2013年2月—2014年2月经外科手术切除及消化内科肠镜活检的部... 目的检测结肠癌组织中NIK和IKKβ连接蛋白(NIBP)、磷酸化p65(p-p65)和C-myc、细胞周期蛋白D1(cyclin D1)的表达,并探讨其意义。方法收集广西医科大学第一附属医院结直肠外科2013年2月—2014年2月经外科手术切除及消化内科肠镜活检的部分标本151例,其中正常结肠黏膜16例、结肠腺瘤21例、结肠癌114例。采用SP免疫组化法分别检测正常结肠黏膜组织、结肠腺瘤组织及结肠癌组织NIBP、p-p65、C-myc和cyclin D1阳性细胞表达率;分析结肠癌组织中NIBP、C-myc和cyclin D1阳性细胞表达率与p-p65阳性细胞表达率的相关性。结果正常结肠黏膜组织、结肠腺瘤组织、结肠癌组织的NIBP、p-p65、C-myc和cyclin D1阳性细胞表达率比较,差异均有统计学意义(P<0.05)。结肠癌组织NIBP、p-p65、C-myc和cyclin D1阳性细胞表达率高于正常结肠黏膜组织和结肠腺瘤组织(P<0.017);正常结肠黏膜组织、结肠腺瘤组织中NIBP、p-p65、C-myc和cyclin D1阳性细胞表达率比较,差异无统计学意义(P>0.017)。不同组织学类型、浸润深度及有无淋巴结转移、远处转移患者结肠癌组织NIBP、p-p65、C-myc和cyclin D1阳性细胞表达率比较,差异有统计学意义(P<0.05)。分化程度越低、TNM分期越高、Duke's分期越高患者结肠癌组织NIBP、p-p65、C-myc和cyclin D1阳性细胞表达率越高(P<0.05)。NIBP、C-myc、cyclin D1阳性细胞表达率与p-p65阳性细胞表达率均呈正相关(P<0.05)。结论 NIBP可能通过激活核因子κB(NF-κB)信号转导通路上调C-myc和cyclin D1等的表达而影响结肠癌的治疗发生、发展、侵袭和转移,为临床发现结肠癌的治疗新靶点提供了参考依据。 展开更多
关键词 结肠肿瘤 NIK和IKKβ连接蛋白 细胞周期蛋白D1 CYCLIN D1
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Abrupt decrease of c-myc expression by antisense transcripts induces terminal differentiation and apoptosis in human promyelocytic leukemia HL-60 cells
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作者 HAO XIU JUAN PEI HSIEN TANG +5 位作者 XIU SEN LI FEI ZI JIANG YONG ZHI XI NING MAO DE LIN DU MIN WU (Institute of Basic Medical Sciences, Beijing 100850)(National Laboratory of Molecular Oncology, In stitute of Cancer Research Beijing, Chinese Academy of Med 《Cell Research》 SCIE CAS CSCD 1996年第2期189-199,共11页
This study was designed using c-myc antisense transcripts to evaluate how alteration of c-myc expression in human myeloid leukemic HL-60 cells could influence the myelomonocytic differentiation and induction of apopto... This study was designed using c-myc antisense transcripts to evaluate how alteration of c-myc expression in human myeloid leukemic HL-60 cells could influence the myelomonocytic differentiation and induction of apoptosis. The recombinant plasmid pDACx expressing antisense transcripts to c-myc fragment containing a part of intron 1 and 137 nt exon 2 was constructed. pDACx was transfected into HL-60 cell line by lipofectin reagent.Cytochemical stainings including NBT reduction, peroxidase and α -NAE as well as detection of CD13 and CD33 antigens by flow cytometric analysis indicated occurrence of myelomonocytic differentiation in cells expressing antisense transcripts to c-myc. DNA degradation measured by DNA gel electrophoresis and typical morphological changes observed under electron microscope proved the switchon of apoptosis in terminally differentiating HL-60 cells. 展开更多
关键词 c-myc oncogene antisense transcripts DIFFERENTIATION APOPTOSIS
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外源性表皮生长因子治疗大鼠慢性胃溃疡对原癌基因表达的影响 被引量:9
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作者 陈宝雯 王洪涛 +2 位作者 刘正新 贾博琦 马清钧 《世界华人消化杂志》 CAS 1999年第6期504-506,共3页
目的通过观察外源性EGF对大鼠实验性胃溃疡治疗后,对胃粘膜组织学及原癌基因cmyc基因及cfos基因表达的影响,探讨EGF在溃疡治疗中应用的可能性.方法乙酸烧灼法制备溃疡模型.术后次日15只以西咪替丁100mg/... 目的通过观察外源性EGF对大鼠实验性胃溃疡治疗后,对胃粘膜组织学及原癌基因cmyc基因及cfos基因表达的影响,探讨EGF在溃疡治疗中应用的可能性.方法乙酸烧灼法制备溃疡模型.术后次日15只以西咪替丁100mg/(kg·d)sc治疗,6只以生理盐水治疗,14只及10只对照组大鼠以EGF10μg/(kg·d)sc治疗,共4wk.于实验的1wk及12wk分别处死动物,进行组织学及原位杂交检查.结果EGF治疗后胃粘膜无异型增生出现.正常胃粘膜cmyc基因表达阴性,cfos基因表达弱阳性;治疗1wk后的溃疡边缘cmyc基因、cfos基因表达弱阳性.实验wk12溃疡治愈后胃粘膜cmyc基因表达阴性,cfos基因表达水平无升高.EGF作用正常胃粘膜后,cmyc基因,cfos基因表达水平无升高.结论外源性EGF长期作用于正常及溃疡状态胃粘膜后。 展开更多
关键词 胃溃疡 表皮生长因子 c-myc原因 C-FOS基因
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Induction of Rat Prolactinoma by β-Estradiol and Its Relation to Expression of c-myc Oncogene
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作者 许荣琨 郭传海 +4 位作者 黄曼影 韩小四 周远征 单惠敏 黄醒亚 《Developmental and Reproductive Biology》 1995年第1期64-69,T001-T002,共8页
Sprague-Dawley rats were implanted with silastic capsules containing β-estradiol. After 60 days, their pituitary weights, serum prolactin contentsand transcription level of c-myc proto-oncogene were found increasedsi... Sprague-Dawley rats were implanted with silastic capsules containing β-estradiol. After 60 days, their pituitary weights, serum prolactin contentsand transcription level of c-myc proto-oncogene were found increasedsignificantly. It was also found that the anterior pituitary cells proliferatedsignificantly, but their differentiation was suppressed. 展开更多
关键词 ESTRADIOL Anterior pituitary tumor PROLACTIN PROTOONCOGENE
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In Vitro transformation of LW13 rat liver epithelial cells
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作者 SHICAN KARLFETNANSKY 《Cell Research》 SCIE CAS CSCD 1992年第2期139-152,共14页
A rat liver epithelial cell line designated LW13 was established using a sequential sedimentation method. The cell line retained many normal properties of liver epithelial cells and showed some structural and function... A rat liver epithelial cell line designated LW13 was established using a sequential sedimentation method. The cell line retained many normal properties of liver epithelial cells and showed some structural and functional features resembling those of liver parenchymal cells. LW13 cells became malignant after the introduction of exogenous transforming EJ Ha ras gene. Tumors produced by inoculation of the transformed cells into baby rats .contained areas of poorly differentiated hepatocellular carcinoma. In situ hybridization analysis confirmed the random rather than specific integration of exogenous ras gene into host chromosomes. Furthermore , an at least tenfold increase in the expression of the endogenous c myc gene was detected among transformed cell lines, suggesting the involvement of the c myc proto oncogene in the in vitro transformation of rat liver epithelial cells by EJ Ha ras oncogene. 展开更多
关键词 LW13-cell line in vitro transformation EJ Ha ras integration c myc expression.
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多态性上皮黏蛋白1和原癌基因C-myc在老年甲状腺乳头状癌患者中的表达 被引量:5
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作者 胡耀杰 罗晓燕 +3 位作者 杨岳 陈春悠 张志勇 郭欣 《中华老年医学杂志》 CAS CSCD 北大核心 2014年第5期496-498,共3页
目的 探讨多态性上皮黏蛋白1 (mucin 1,MUC1)和原癌基因蛋白质(proto-oncogene proteins C-myc,C-Myc)基因在老年甲状腺乳头状癌(PTC)患者中的表达和临床意义. 方法 应用免疫组化法检测30例腺瘤旁正常甲状腺组织、35例结节性甲状... 目的 探讨多态性上皮黏蛋白1 (mucin 1,MUC1)和原癌基因蛋白质(proto-oncogene proteins C-myc,C-Myc)基因在老年甲状腺乳头状癌(PTC)患者中的表达和临床意义. 方法 应用免疫组化法检测30例腺瘤旁正常甲状腺组织、35例结节性甲状腺肿和58例PTC标本中MUC1和C-myc的表达水平. 结果 MUC1基因在甲状腺癌中表达率为77.6%(45/58),MUC1在有局部浸润和淋巴结转移患者中的检出率分别为90.0%(9/10)和88.2%(15/17).C-myc基因在甲状腺癌中阳性率为81.0% (47/58);C-myc在淋巴结转移组中的检出率为100.0% (17/17). 结论 MUC1和C myc的阳性表达在甲状腺良恶性病变间有差异,并与甲状腺癌的转移有关. 展开更多
关键词 乳头状 甲状腺肿瘤 黏蛋白类 Mucins1
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Regulating Effects of Herb Cake-partitioned Moxibustion on the Expression of p53 and C-myc Protein in Rats with Ulcerative Colitis 被引量:4
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作者 赵天平 王晓梅 +2 位作者 刘慧荣 吴焕淦 周爽 《Journal of Acupuncture and Tuina Science》 2010年第3期154-158,共5页
Objective: To investigate the mechanism of herb cake-partitioned moxibustion treating ulcerative colitis (UC) from the relationship between expression of p53 and C-myc protein, and morbidity of UC. Methods: Rats m... Objective: To investigate the mechanism of herb cake-partitioned moxibustion treating ulcerative colitis (UC) from the relationship between expression of p53 and C-myc protein, and morbidity of UC. Methods: Rats model of UC was made with immune methods and local stimulation. Forty SD rats were divided into normal, model, herb cake-partitioned, and mild moxibustion group by a random number table, 10 rats in each group. Hanging moxibustion in the mild moxibustion group was applied to Tianshu (ST 25) and Qihai (CV 6) for 10 rain. Two moxa cones of herb cake-partitioned moxibustion were applied to the same acupoints respectively, in the herb cake-partitioned moxibustion group. Expression of p53 and C-myc protein was measured with immuno- histochemistical method in the colonic tissue of rats with UC. Results: Postive area, strength, and the immunohistochemistry index of the expression of p53 and C-myc protein were found more in the model rats than those in the normal rats (P〈0.01), whereas less in the herb cake-partitioned moxibustion and mild moxibustion groups than those in the model group (P〈0.01). Conclusion: p53 and C-myc play important roles in the morbidity and development of UC, and herb cake-partitioned moxibustion could regulate the expression of p53 and C-myc protein in the colonic tissue of UC rats. 展开更多
关键词 Colitis Ulcerative Indirect Moixbustion Genes p53 Proto-Oncogene Proteins c-myc
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