Objective:To investigate the regulatory role of cyclic adenosine monophosphate responsive element binding protein(CREB)/brain-derived neurotrophic factor(BDNF)signaling pathway in acute sleep deprivation(SD)-induced a...Objective:To investigate the regulatory role of cyclic adenosine monophosphate responsive element binding protein(CREB)/brain-derived neurotrophic factor(BDNF)signaling pathway in acute sleep deprivation(SD)-induced anxiety-like behavior mice(SD group)to study the mechanism of anxiety-like behavior better.Methods:The SD chamber was used to deprive the mice of sleep,and the anxiety-like behavior of the mice was verified using an open field test(OFT),elevated plus maze(EPM),forced swim test(FST),and tail suspension test(TST).Finally,proteins were detected by Western blotting.Result:OFT showed that the active distance and the time of stay in the central area were significantly reduced(P<0.05).EPM showed that the time and number of open arms in the SD group were significantly lower than in the control group(P<0.05).The FST showed that the forced swimming immobility time of the SD group was significantly lower than that of the control(P<0.05).Moreover,the TST showed that the immobility time of the tail suspension experiment in the SD group was significantly higher than that in the control group(P<0.05).Conclusion:Acute SD can regulate anxiety-like behavior in mice through the CREB/BDNF signaling pathway.展开更多
脑源性神经营养因子(B ra in-derived neurotroph ic factor,BDNF)是脑组织中含量最丰富的神经营养因子而且可能为一活性依赖性神经元存活因子。体外试验证实BDNF对正常皮质神经元有维持存活效应;对缺氧神经元有确切保护作用。它主要通...脑源性神经营养因子(B ra in-derived neurotroph ic factor,BDNF)是脑组织中含量最丰富的神经营养因子而且可能为一活性依赖性神经元存活因子。体外试验证实BDNF对正常皮质神经元有维持存活效应;对缺氧神经元有确切保护作用。它主要通过丝裂原活化蛋白激酶(M itogen-activated prote in k inase,M APK)信号通路及C a2+/钙调蛋白依赖性激酶(C a2+/C a lm odu lin-dependen t k inase,C aM K)信号通路调节脑细胞的生长、分化、存活、抗凋亡作用,而且其信号传导通路之间存在着复杂的交叉串扰机制。展开更多
文摘Objective:To investigate the regulatory role of cyclic adenosine monophosphate responsive element binding protein(CREB)/brain-derived neurotrophic factor(BDNF)signaling pathway in acute sleep deprivation(SD)-induced anxiety-like behavior mice(SD group)to study the mechanism of anxiety-like behavior better.Methods:The SD chamber was used to deprive the mice of sleep,and the anxiety-like behavior of the mice was verified using an open field test(OFT),elevated plus maze(EPM),forced swim test(FST),and tail suspension test(TST).Finally,proteins were detected by Western blotting.Result:OFT showed that the active distance and the time of stay in the central area were significantly reduced(P<0.05).EPM showed that the time and number of open arms in the SD group were significantly lower than in the control group(P<0.05).The FST showed that the forced swimming immobility time of the SD group was significantly lower than that of the control(P<0.05).Moreover,the TST showed that the immobility time of the tail suspension experiment in the SD group was significantly higher than that in the control group(P<0.05).Conclusion:Acute SD can regulate anxiety-like behavior in mice through the CREB/BDNF signaling pathway.
文摘脑源性神经营养因子(B ra in-derived neurotroph ic factor,BDNF)是脑组织中含量最丰富的神经营养因子而且可能为一活性依赖性神经元存活因子。体外试验证实BDNF对正常皮质神经元有维持存活效应;对缺氧神经元有确切保护作用。它主要通过丝裂原活化蛋白激酶(M itogen-activated prote in k inase,M APK)信号通路及C a2+/钙调蛋白依赖性激酶(C a2+/C a lm odu lin-dependen t k inase,C aM K)信号通路调节脑细胞的生长、分化、存活、抗凋亡作用,而且其信号传导通路之间存在着复杂的交叉串扰机制。
