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Chinese Cabbage-pak-choi Transcriptome Map Construction with cDNA-AFLP Techniques 被引量:1
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作者 FAN Shu-ying LE Jian-gang CHENG Guang-jie WU Cai-jun 《Agricultural Sciences in China》 CAS CSCD 2008年第10期1181-1188,共8页
Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino) transcriptome map with cDNA-AFLP techniques was constructed. The inbred line Aijiaohuang 97-3-2, the inbred line Baimanjing 001-24 of turnip [B... Chinese cabbage-pak-choi (Brassica campestris L. ssp. chinensis Makino) transcriptome map with cDNA-AFLP techniques was constructed. The inbred line Aijiaohuang 97-3-2, the inbred line Baimanjing 001-24 of turnip [B. campestris ssp. rapifera (Matzg.) Sinsk] and 183 F6 (recombinant inbred population) plants were used as experimental materials, cDNAs were synthesized from total RNA extracted from young leaves at rosette stage. 256 pairs of cDNA-AFLP primers were used to detect the polymorphisms between parents Aijiaohuang 97-3-2 and Baimanjing 001-24. 56 pairs of cDNA-AFLP primers with high polymorphisms were screened from 256 pairs of primer by DNA-AFLP techniques. The genetic diversity of parents and 183 F6 progenies was detected by 56 pairs of cDNA-AFLP primers. The segregation and distribution of cDNA-AFLPs molecular marker were analyzed to construct transcriptome map amongst parents and F6 plants. A total of 164 cDNA-AFLPs marker loci were mapped into 13 linkage groups which covered 1401.2 cM with an average distance of 9.7 cM. It was the first transcriptome map of Chinese cabbage using cDNA-AFLP technique. 展开更多
关键词 Brassica campestris ssp. chinensis cdna-aflp transcriptome map
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cDNA-AFLP profiling for the fiber development stage of secondary cell wall synthesis and transcriptome mapping in cotton 被引量:5
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作者 PAN YuXin MA Jun ZHANG GuiYin HAN GaiYing WANG XingFent MA ZhiYingt 《Chinese Science Bulletin》 SCIE EI CAS 2007年第17期2358-2364,共7页
Cotton fiber strength is mainly determined during the secondary cell wall deposition stage when cel- lulose is synthesized. We obtained cDNA of 20―25 d post anthesis (DPA) fiber from 109 F2 progeny and developed a co... Cotton fiber strength is mainly determined during the secondary cell wall deposition stage when cel- lulose is synthesized. We obtained cDNA of 20―25 d post anthesis (DPA) fiber from 109 F2 progeny and developed a cotton fiber transcriptome profiling via cDNA-AFLP technology using 37 different primer combinations. The F2 population originated from an interspecific cross between Gossypium hirsutum and Gossypium barbadense. One hundred and thirty-eight absence/presence polymorphic transcript- derived fragments (TDFs), with sizes ranging from 100 bp to 722 bp, were screened. Of these, 75 (53.62%) were polymorphic between the parents of the F2 population. Sequencing the 75 transcripts revealed that 37 of them had been reported to be cotton fiber ESTs. Nine of 75 transcript sequences were homologous to 7 cloned cotton fiber genes, encoding cysteine proteinase, vacuolar H+-pyro- phosphatase, vacuolar H+-ATPase, catalytic subunit, arabinogalactan protein, putative receptor protein kinase PERK1, GIA/RGA-like gibberellin response modulator and cellulose synthase. Some other transcripts may represent new gene fragments in cotton fiber development. Surprisingly, 46 of the 75 transcripts were mapped to a single linkage group. The transcriptome groups and the sequenced TDFs could serve as important resources in the functional genomic research of cotton fiber development. 展开更多
关键词 棉花 cdna-aflp 基因转录 纤维生长 细胞壁
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用cDNA-AFLP技术构建基因组转录图谱 被引量:16
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作者 付凤玲 李晚忱 《分子植物育种》 CAS CSCD 2003年第4期523-529,共7页
以分离群体mRNA反转录的cDNA为模板进行AFLP分析 ,在保留AFLP多态性丰富、稳定性高、无需了解序列信息等优点的同时 ,集中显示基因组表达序列的多态性差异 ,已逐渐发展成为基因差异表达显示、表达基因遗传连锁作图和基因克隆的常用方法... 以分离群体mRNA反转录的cDNA为模板进行AFLP分析 ,在保留AFLP多态性丰富、稳定性高、无需了解序列信息等优点的同时 ,集中显示基因组表达序列的多态性差异 ,已逐渐发展成为基因差异表达显示、表达基因遗传连锁作图和基因克隆的常用方法。本文介绍了作图群体组配、总RNA提取与mRNA分离、cDNA合成、AFLP分析、转录图谱多态性分析作图等cDNA AFLP分析的主要技术。 展开更多
关键词 基因组转录图谱 cdnaaflp技术 作图群体组配 总RNA提取 mRNA分离 cdna合成
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用cDNA-AFLP技术构建白菜转录图谱 被引量:2
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作者 范淑英 乐建刚 +1 位作者 成广杰 吴才君 《中国农业科学》 CAS CSCD 北大核心 2008年第6期1735-1741,共7页
【目的】构建白菜(Brasscia campestris ssp.chinensis)分子转录图谱。【方法】以矮脚黄白菜自交系(97-3-2)和白蔓菁芜菁自交系(001-24),以及以两者为亲本杂交建立的F6重组自交系群体为材料,在莲座期取嫩叶提取RNA并反转录成cDNA,采用cD... 【目的】构建白菜(Brasscia campestris ssp.chinensis)分子转录图谱。【方法】以矮脚黄白菜自交系(97-3-2)和白蔓菁芜菁自交系(001-24),以及以两者为亲本杂交建立的F6重组自交系群体为材料,在莲座期取嫩叶提取RNA并反转录成cDNA,采用cDNA-AFLP技术,利用256对cDNA-AFLP引物对亲本97-3-2、001-24之间的多态性进行检测,筛选出56对多态性引物用于F6群体分析,通过对cDNA-AFLPs分子标记的遗传分析构建图谱。【结果】构建了一个包括164个cDNA-AFLPs分子标记的分子转录图谱。该图谱由13个连锁群组成,图谱总长度为1401.2cM,标记间平均图距9.7cM。【结论】这是第一份利用cDNA-AFLP标记构建的白菜分子转录图谱。 展开更多
关键词 白菜 cdna-aflp 转录图谱
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Mining cotton fiber strength candidate genes based on transcriptome mapping 被引量:1
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作者 LIU HengWei WANG XingFen +3 位作者 PAN YuXin SHI RuiFeng ZHANG GuiYin MA ZhiYing 《Chinese Science Bulletin》 SCIE EI CAS 2009年第24期4651-4657,共7页
Cotton fiber strength is mainly determined during the secondary cell wall (SCW) thickening stage. In 24―25 days post anthesis (DPA) of SCW thickening stage, cDNA-amplified fragment length polymorphism (AFLP) was carr... Cotton fiber strength is mainly determined during the secondary cell wall (SCW) thickening stage. In 24―25 days post anthesis (DPA) of SCW thickening stage, cDNA-amplified fragment length polymorphism (AFLP) was carried out to construct fiber transcriptome groups. Based on these groups, cotton fiber strength candidate genes were detected by composite interval mapping (CIM) through quantitative trait locus (QTL) scanning. The mapping population was the interspecific backcross BC1 of Gossypium hirsutum × G. barbadense. One hundred and fifteen BC1 plants were used for group construction with 102 qualified absence/presence polymorphic transcript-derived fragments (TDFs) from G. barbadense, and 78 TDFs were assigned into eight transcriptome groups that gave a total length of 462.63 centimorgans (cM). Two significant QTLs, FS1 and FS2, were detected and explained 16.08% and 15.87% of fiber strength variance, respectively. Of the six TDFs co-segregating with FS1 and FS2, except one encoding an unknown protein, five targeted putative phosphatidylinositol kinase, trehalose-6 phosphate synthase, MADS transcription factor, cellulose synthase-like protein and phenylalanine ammonia lyase, respectively. These functional genes were involved in plant cell wall morphogenesis or cellulose synthesis metabolism processes, and were considered as the candidate genes controlling cotton fiber strength. 展开更多
关键词 纤维比强度 基因转录 扩增片段长度多态性 棉花 映射 候选人 数量性状位点 复合区间作图法
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