AIM: To investigate the immunogenicity of a novel DNA vaccine,pSW3891/HBc, based on HBV core gene in Balb/c mice.METHODS: A novel DNA vaccine, pSW3891/HBc, encoding HBV core gene was constructed using a vector plasmid...AIM: To investigate the immunogenicity of a novel DNA vaccine,pSW3891/HBc, based on HBV core gene in Balb/c mice.METHODS: A novel DNA vaccine, pSW3891/HBc, encoding HBV core gene was constructed using a vector plasmid pSW3891. Balb/c mice were immunized with either pSW3891/HBc or empty vector DNA via gene gun. IgG anti-HBc responses in mouse sera were demonstrated by ELISA. Specific cytotoxicity of cytotoxic T lymphocytes (CTLs) of mice was quantitatively measured by lactate dehydrogenase release assay.RESULTS: HBcAg was expressed effectively in 293T cell line transiently transfected with pSW3891/HBc. Strong IgG anti-HBc responses were elicited in mice immunized with pSW3891/HBc. The end-point titers of anti-HBc reached the highest 1:97 200, 4 wk after the third immunization. The specific CTL killing with the highest specific lysis reached 73.25% at effector:target ratio of 20:1 in mice that received pSW3891/HBc DNA vaccine.CONCLUSION: pSW3891/HBc vaccination elicits specific anti-HBc response and induces HBc-specific CTL response in immunized Balb/c mice.展开更多
为了提高电子束偏转扫描频率和磁场均匀性,采用Helmholtz空心线圈结构设计了一种低感抗电子枪偏转扫描线圈。采用ANSYS有限元分析软件对偏转扫描线圈的电磁场分布进行仿真,并采用高斯计对线圈内部的电磁场进行了测量,仿真结果与实际测...为了提高电子束偏转扫描频率和磁场均匀性,采用Helmholtz空心线圈结构设计了一种低感抗电子枪偏转扫描线圈。采用ANSYS有限元分析软件对偏转扫描线圈的电磁场分布进行仿真,并采用高斯计对线圈内部的电磁场进行了测量,仿真结果与实际测量值基本吻合,线圈内部的磁感应强度大小和电磁场分布的均匀性可以满足电子束高频偏转扫描的要求。实际扫描试验表明,该线圈实现了两点电子束高频偏转扫描,扫描频率可达30 k Hz。展开更多
基金Supported by the 135 Project of Jiangsu Province, No. 044
文摘AIM: To investigate the immunogenicity of a novel DNA vaccine,pSW3891/HBc, based on HBV core gene in Balb/c mice.METHODS: A novel DNA vaccine, pSW3891/HBc, encoding HBV core gene was constructed using a vector plasmid pSW3891. Balb/c mice were immunized with either pSW3891/HBc or empty vector DNA via gene gun. IgG anti-HBc responses in mouse sera were demonstrated by ELISA. Specific cytotoxicity of cytotoxic T lymphocytes (CTLs) of mice was quantitatively measured by lactate dehydrogenase release assay.RESULTS: HBcAg was expressed effectively in 293T cell line transiently transfected with pSW3891/HBc. Strong IgG anti-HBc responses were elicited in mice immunized with pSW3891/HBc. The end-point titers of anti-HBc reached the highest 1:97 200, 4 wk after the third immunization. The specific CTL killing with the highest specific lysis reached 73.25% at effector:target ratio of 20:1 in mice that received pSW3891/HBc DNA vaccine.CONCLUSION: pSW3891/HBc vaccination elicits specific anti-HBc response and induces HBc-specific CTL response in immunized Balb/c mice.
文摘为了提高电子束偏转扫描频率和磁场均匀性,采用Helmholtz空心线圈结构设计了一种低感抗电子枪偏转扫描线圈。采用ANSYS有限元分析软件对偏转扫描线圈的电磁场分布进行仿真,并采用高斯计对线圈内部的电磁场进行了测量,仿真结果与实际测量值基本吻合,线圈内部的磁感应强度大小和电磁场分布的均匀性可以满足电子束高频偏转扫描的要求。实际扫描试验表明,该线圈实现了两点电子束高频偏转扫描,扫描频率可达30 k Hz。