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细胞周期相关新基因CACUL1对培养的结直肠癌细胞凋亡的影响 被引量:2
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作者 孔莺 亓小改 +1 位作者 白沛松 南克俊 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2013年第5期462-464,468,共4页
目的研究细胞周期相关新基因细胞周期素依赖激酶2相关性Culin结构域1(CACUL1)对肿瘤细胞凋亡的影响,探讨CACUL1与细胞凋亡调控的关系。方法用紫外线及化疗药物诱导细胞周期的阻滞,研究CACUL1在细胞DNA损伤状态下表达情况;同时用p53野生... 目的研究细胞周期相关新基因细胞周期素依赖激酶2相关性Culin结构域1(CACUL1)对肿瘤细胞凋亡的影响,探讨CACUL1与细胞凋亡调控的关系。方法用紫外线及化疗药物诱导细胞周期的阻滞,研究CACUL1在细胞DNA损伤状态下表达情况;同时用p53野生型及敲除的结直肠癌细胞,应用Western blot、Northern blot等方法检测了CACUL1蛋白及mRNA表达与p53的关系。结果与细胞凋亡的调控有关,在紫外线及化疗药物诱导的DNA损伤情况下,CACUL1蛋白的表达在2 h达最高,随着时间的逐渐延长,CACUL1的表达逐渐减低,而这种表达的增高是p53非依赖性的,Northern blot结果显示CACUL1的表达增高是转录后调控机制。CACUL1的高表达能够抑制紫外线及化疗药物诱导的细胞凋亡。结论 CACUL1在细胞凋亡中的作用是以一种转录后调控的p53非依赖方式帮助细胞跨过G1/S检控点,利于细胞生存。 展开更多
关键词 CACUL1 细胞周期 细胞凋亡
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沉默CACUL1表达对食管癌细胞增殖的影响及其机制 被引量:3
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作者 马海琳 车少敏 +2 位作者 王晓丽 张晓智 党诚学 《山西医科大学学报》 CAS 2018年第4期347-351,共5页
目的探讨沉默CACUL1表达对食管癌细胞增殖的影响及机制,为食管癌的基因治疗提供实验依据。方法合成CACUL1 siRNA,转染EC109细胞,实时定量PCR检测转染CACUL1 siRNA前后CACUL1 mRNA表达变化,MTT法检测EC109细胞增殖能力,FCM检测细胞周期... 目的探讨沉默CACUL1表达对食管癌细胞增殖的影响及机制,为食管癌的基因治疗提供实验依据。方法合成CACUL1 siRNA,转染EC109细胞,实时定量PCR检测转染CACUL1 siRNA前后CACUL1 mRNA表达变化,MTT法检测EC109细胞增殖能力,FCM检测细胞周期和凋亡,Western blot法分析CACUL1 siRNA对CDK2-Cyclin A/E蛋白表达的影响。结果通过转染CACUL1 siRNA沉默CACUL1,转染后EC109细胞中CACUL1 mRNA和蛋白表达量均较转染前显著降低(P<0.01)。MTT检测结果显示,转染后EC109细胞增殖能力受到显著抑制(P<0.01),FCM检测显示细胞周期阻滞在G_1/G_0期(88.56±4.96,P<0.01),早期凋亡和晚期凋亡细胞数量均显著增加(P<0.01)。Western blot实验结果显示,CDK2-Cyclin A/E蛋白表达量显著下调。结论 CACUL1通过调控CDK2-Cyclin A/E信号通路促进食管癌EC109细胞增殖。 展开更多
关键词 食管癌 CACUL1 CDK2-Cyclin A/E 细胞增殖
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The effect of phase angle and wing spacing on tandem flapping wings 被引量:12
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作者 Timothy M. Broering Yong-Sheng Lian 《Acta Mechanica Sinica》 SCIE EI CAS CSCD 2012年第6期1557-1571,共15页
In a tandem wing configuration, the hindwing of- ten operates in the wake of the forewing and, hence, its per- formance is affected by the vortices shed by the forewing. Changes in the phase angle between the flapping... In a tandem wing configuration, the hindwing of- ten operates in the wake of the forewing and, hence, its per- formance is affected by the vortices shed by the forewing. Changes in the phase angle between the flapping motions of the fore and the hind wings, as well as the spacing between them, can affect the resulting vortex/wing and vortex/vortex interactions. This study uses 2D numerical simulations to in- vestigate how these changes affect the leading dege vortexes (LEV) generated by the hindwing and the resulting effect on the lift and thrust coefficients as well as the efficiencies. The tandem wing configuration was simulated using an incom- pressible Navier-Stokes solver at a chord-based Reynolds number of 5 000. A harmonic single frequency sinusoidal oscillation consisting of a combined pitch and plunge motion was used for the flapping wing kinematics at a Strouhal num- ber of 0.3. Four different spacings ranging from 0.1 chords to 1 chord were tested at three different phase angles, 0°, 90° and 180°. It was found that changes in the spacing and phase angle affected the timing of the interaction between the vor- tex shed from the forewing and the hindwing. Such an inter- action affects the LEV formation on the hindwing and results in changes in aerodynamic force production and efficiencies of the hindwing. It is also observed that changing the phase angle has a similar effect as changing the spacing. The re- suits further show that at different spacings the peak force generation occurs at different phase angles, as do the peak efficiencies. 展开更多
关键词 Tandem. Flapping. Airfoils. Micro air vehicle.Caculational fluid dynamics (CFD) Overlapping grids
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