AIM To evaluate effect of treatment failure on cag A and vac A genotypes in Helicobacter pylori(H. pylori) isolates from Colombia.METHODS One hundred and seventy-six participants infected with H. pylori from Colombia ...AIM To evaluate effect of treatment failure on cag A and vac A genotypes in Helicobacter pylori(H. pylori) isolates from Colombia.METHODS One hundred and seventy-six participants infected with H. pylori from Colombia were treated during 14 d with the triple-standard therapy. Six weeks later, eradication was evaluated by 13C-Urea breath test. Patients with treatment failure were subjected to endoscopy control; biopsies obtained were used for histopathology and culture. DNA from H. pylori isolates was amplified using primers specific for cag A and vac A genes. The phylogenetic relationships among isolates obtained before and after treatment were established by conglomerate analysis based on random amplified polymorphic DNA(RAPD) fingerprinting.RESULTS Treatment effectiveness was at 74.6%. Of the par-ticipants with treatment failure, 25 accepted subjected to a second endoscopy. Prevalence of posttreatment infection was 64%(16/25) and 40%(10/25) by histology and culture, respectively. Upon comparing the cag A and vac A genotypes found before and after therapy, multiple cag A genotypes(cag A-positive and cag A-negative) were found before treatment; in contrast, cag A-negative genotypes decreased after treatment. vac A s1m1 genotype was highly prevalent in patients before and after therapy. The 3'cag A region was successfully amplified in 95.5%(21/22) of the isolates obtained before and in 81.8%(18/22) of the isolates obtained after treatment. In the isolates obtained from patients with treatment failure, it was found that 72.7%(16/22) presented alterations in the number of EPIYA motifs, compared to isolates found before treatment.CONCLUSION Unsuccessful treatment limits colonization by lowvirulence strains resulting in partial and selective eradication in mixed infections, and acts on the cag A-positive strains inducing genetic rearrangements in cag A variable region that produces a loss or gain of EPIYA repetitions.展开更多
To setup a method of amplification for the whole Cag A gene of H elicobacter pylori and its fingerprinting by restriction fragm entlength polym orphism(RFL P) ,nested PCR was employed in com bination with TD- PCR to...To setup a method of amplification for the whole Cag A gene of H elicobacter pylori and its fingerprinting by restriction fragm entlength polym orphism(RFL P) ,nested PCR was employed in com bination with TD- PCR to am plify the gene and Eco RΙ and Hind were used to generate the RFL P fingerprinting.Target DNA fragm ents from 13of2 0 samples were successfully amplified and the relevant RFL P fingerprintings were obtained.It is concluded thatthe m ethod can be used to amplify the whole Cag A gene and Cag A gene has apparent diversity of RFL P profile.展开更多
Objective:To determine the association of smoking, alcohol and nonsteroidal anti-inflammatory drugs(NSAIDs) use with presence and virulence of Helicobacter pylori(H. pylori) infection in a representative sample of a r...Objective:To determine the association of smoking, alcohol and nonsteroidal anti-inflammatory drugs(NSAIDs) use with presence and virulence of Helicobacter pylori(H. pylori) infection in a representative sample of a random adult population of asymptomatic subjects.Methods:Non virulent 16S rRNA and virulent cag A and T genes from salivary samples of 854 asymptomatic subjects were determined using polymerase chain reaction. The presence and absence of virulent and non virulent infection was statistically compared with consumption of smoking, alcohol and NSAID s.Results:The prevalence of infection in male and female subjects was found to be 69.25% and 66.90%, respectively. The prevalence of infection in the population of asymptomaticsubjects with respect to consumption of alcohol was as follows: current(31.22%), former(52.20%) and never(43.58%). The prevalence of infection in the population of asymptomatic subjects withrespect to smoking of cigarettes was as follows: current(88.80%), former(57.14%) and never(33.33%).The prevalence of infection in the subject population consuming NSAIDs and not consuming NSAIDs frequently was found to be 82.75%and 21.16%, respectively. Virulence in male and femalesubjects was found to be 60.00%and 50.00%, respectively. The presence of virulent infection in the population of asymptomatic subjects with respect to consumption of alcohol was as follows:current(28.57%), former(40.15%) and never(50.00%). The prevalence of virulent infection in the population of asymptomatic subjects with respect to smoking of cigarettes was as follows: current(79.32%), former(75.00%) and never(50.00%). The prevalence of virulent infection in the subject population consuming NSAIDs and not consuming NSAIDs frequently was found to be 88.23% and 66.66%, respectively.Conclusions:It can be concluded that smoking and NSAIDs consumption are aggravating factors for virulence ofH. pylori and alcohol can inhibitH. pyloriin fection in asymptomatic subjects.展开更多
Aim: The Androgen Receptor (AR) is a ligand-dependent transcriptional activator and the AR gene contains a highly polymorphic trinucleotide repeat CAG and GGN in the first exon. Given the lack of information AR-CAG an...Aim: The Androgen Receptor (AR) is a ligand-dependent transcriptional activator and the AR gene contains a highly polymorphic trinucleotide repeat CAG and GGN in the first exon. Given the lack of information AR-CAG and GGN repeat polymorphism and its potential correlation with breast cancer in South Indian women, we conducted a case-control study to observe the effects of CAG & GGN repeat length polymorphism and risk of breast cancer. Methods: Polymorphisms for AR-CAG and GGN repeat length was detected by Gene Scan analysis in the genomic DNA from cases with breast cancer and controls. Results: Association between AR genotype was calculated by categorising alleles as short (S) and long (L) and taking median value as the cut-off. LL genotype of CAG repeat was found to be associated with breast cancer (OR, 4.58;95% CI, 10.61-1.98;p—0.0004). GGN repeat having ≥21 was found in most of the cases and none of the cases showed 20 repeats thus indicate that alleles having homozygous repeat 20 may be protective towards breast cancer. Also, SS genotype was observed in 56.84% of cases and in 73.03% of controls (OR, 0.48;95% CI, 0.26-0.89;p value, 0.02). Conclusion: Our results indicate that longer CAG and GGN repeat may be associated with breast cancer whereas, the shorter GGN repeat length genotype of AR are protective.展开更多
文摘AIM To evaluate effect of treatment failure on cag A and vac A genotypes in Helicobacter pylori(H. pylori) isolates from Colombia.METHODS One hundred and seventy-six participants infected with H. pylori from Colombia were treated during 14 d with the triple-standard therapy. Six weeks later, eradication was evaluated by 13C-Urea breath test. Patients with treatment failure were subjected to endoscopy control; biopsies obtained were used for histopathology and culture. DNA from H. pylori isolates was amplified using primers specific for cag A and vac A genes. The phylogenetic relationships among isolates obtained before and after treatment were established by conglomerate analysis based on random amplified polymorphic DNA(RAPD) fingerprinting.RESULTS Treatment effectiveness was at 74.6%. Of the par-ticipants with treatment failure, 25 accepted subjected to a second endoscopy. Prevalence of posttreatment infection was 64%(16/25) and 40%(10/25) by histology and culture, respectively. Upon comparing the cag A and vac A genotypes found before and after therapy, multiple cag A genotypes(cag A-positive and cag A-negative) were found before treatment; in contrast, cag A-negative genotypes decreased after treatment. vac A s1m1 genotype was highly prevalent in patients before and after therapy. The 3'cag A region was successfully amplified in 95.5%(21/22) of the isolates obtained before and in 81.8%(18/22) of the isolates obtained after treatment. In the isolates obtained from patients with treatment failure, it was found that 72.7%(16/22) presented alterations in the number of EPIYA motifs, compared to isolates found before treatment.CONCLUSION Unsuccessful treatment limits colonization by lowvirulence strains resulting in partial and selective eradication in mixed infections, and acts on the cag A-positive strains inducing genetic rearrangements in cag A variable region that produces a loss or gain of EPIYA repetitions.
基金Thisprojectwassupported by a grant from the MinistryofPublicHealth(Serial No.:98- 1- 12 3)
文摘To setup a method of amplification for the whole Cag A gene of H elicobacter pylori and its fingerprinting by restriction fragm entlength polym orphism(RFL P) ,nested PCR was employed in com bination with TD- PCR to am plify the gene and Eco RΙ and Hind were used to generate the RFL P fingerprinting.Target DNA fragm ents from 13of2 0 samples were successfully amplified and the relevant RFL P fingerprintings were obtained.It is concluded thatthe m ethod can be used to amplify the whole Cag A gene and Cag A gene has apparent diversity of RFL P profile.
基金financially supported by India Council of Technical Education as National Doctoral Fellowship(grant No.RID/NDF-37/2009/10)
文摘Objective:To determine the association of smoking, alcohol and nonsteroidal anti-inflammatory drugs(NSAIDs) use with presence and virulence of Helicobacter pylori(H. pylori) infection in a representative sample of a random adult population of asymptomatic subjects.Methods:Non virulent 16S rRNA and virulent cag A and T genes from salivary samples of 854 asymptomatic subjects were determined using polymerase chain reaction. The presence and absence of virulent and non virulent infection was statistically compared with consumption of smoking, alcohol and NSAID s.Results:The prevalence of infection in male and female subjects was found to be 69.25% and 66.90%, respectively. The prevalence of infection in the population of asymptomaticsubjects with respect to consumption of alcohol was as follows: current(31.22%), former(52.20%) and never(43.58%). The prevalence of infection in the population of asymptomatic subjects withrespect to smoking of cigarettes was as follows: current(88.80%), former(57.14%) and never(33.33%).The prevalence of infection in the subject population consuming NSAIDs and not consuming NSAIDs frequently was found to be 82.75%and 21.16%, respectively. Virulence in male and femalesubjects was found to be 60.00%and 50.00%, respectively. The presence of virulent infection in the population of asymptomatic subjects with respect to consumption of alcohol was as follows:current(28.57%), former(40.15%) and never(50.00%). The prevalence of virulent infection in the population of asymptomatic subjects with respect to smoking of cigarettes was as follows: current(79.32%), former(75.00%) and never(50.00%). The prevalence of virulent infection in the subject population consuming NSAIDs and not consuming NSAIDs frequently was found to be 88.23% and 66.66%, respectively.Conclusions:It can be concluded that smoking and NSAIDs consumption are aggravating factors for virulence ofH. pylori and alcohol can inhibitH. pyloriin fection in asymptomatic subjects.
文摘Aim: The Androgen Receptor (AR) is a ligand-dependent transcriptional activator and the AR gene contains a highly polymorphic trinucleotide repeat CAG and GGN in the first exon. Given the lack of information AR-CAG and GGN repeat polymorphism and its potential correlation with breast cancer in South Indian women, we conducted a case-control study to observe the effects of CAG & GGN repeat length polymorphism and risk of breast cancer. Methods: Polymorphisms for AR-CAG and GGN repeat length was detected by Gene Scan analysis in the genomic DNA from cases with breast cancer and controls. Results: Association between AR genotype was calculated by categorising alleles as short (S) and long (L) and taking median value as the cut-off. LL genotype of CAG repeat was found to be associated with breast cancer (OR, 4.58;95% CI, 10.61-1.98;p—0.0004). GGN repeat having ≥21 was found in most of the cases and none of the cases showed 20 repeats thus indicate that alleles having homozygous repeat 20 may be protective towards breast cancer. Also, SS genotype was observed in 56.84% of cases and in 73.03% of controls (OR, 0.48;95% CI, 0.26-0.89;p value, 0.02). Conclusion: Our results indicate that longer CAG and GGN repeat may be associated with breast cancer whereas, the shorter GGN repeat length genotype of AR are protective.
文摘目的探究阿糖胞苷、阿克拉霉素、粒细胞集落刺激因子(cytarabine,aclarubicin,granulocyte colony stimulating factor,CAG)化疗结合中医健脾益肾治疗老年急性髓系白血病的疗效。方法以2017年1月—2022年1月在广州市第一人民医院确诊为急性髓系白血病(acute myeloid leukemia,AML)的60例老年患者为研究对象,将其随机分为单用化疗组和联合治疗组,每组30例。单用化疗组采用CAG化疗治疗,中西医结合组在此基础上服用健脾益肾中药汤剂进行补充治疗。比较两组治疗后的疗效及不良反应发生情况,观察两组患者骨髓恢复时间、血液指标和血液成分输入量,评价两组治疗后的中医症候积分及生活质量。结果联合治疗组总有效率、中医症候积分及癌症患者生活质量量表(functional assessment of cancer therapy-general,FACT-G)得分明显优于单独化疗组(P<0.05);联合治疗组感染、出血、胃肠道反应和骨髓抑制等不良反应发生率明显低于单独化疗组(P<0.05);联合治疗组骨髓恢复时间明显短于单独化疗组,血小板含量较单独化疗组高,而输注的红细胞与血小板数量明显低于单独化疗组(P<0.05)。结论CAG化疗结合健脾益肾治疗对老年AML患者的症状缓解可能有更好的效果,且能有效改善感染等不良反应的发生及血液制品的输注,使其拥有相对良好的生活质量。
