Variations in Caladium humboldtii cv. ＇Phraya Savet＇ from in vitro culture were observed. Callus and small shoots were induced from unexpanded leaf segments cultured on modified MS medium supplemented with 2.69 p.M ...Variations in Caladium humboldtii cv. ＇Phraya Savet＇ from in vitro culture were observed. Callus and small shoots were induced from unexpanded leaf segments cultured on modified MS medium supplemented with 2.69 p.M l-Naphthalene acetic acid （NAA） and 17.76 μM N6-Benzyladenine （BA）. Shoots were transferred onto modified MS medium supplemented with 8.88 μM BA for shoot multiplication. Subsequently, roots were induced on MS without growth regulator. The regenerated plantlets were vigorously grown in glasshouse conditions. From 4 morphological groups （leaf color ratio, leaf color, petiole and leaf pattern）, the regenerated ＇Phraya Savet＇ caladium plants were divided into 6 types. The occurrence of variants was 52%. Most of the mutated plants were observed from only leaf color ratio （green ： white） and leaf shape. The most significance for commercial value from mutated clones was round leaf obtaining 20%. Characteristics of new clones from somatic hybridization between C humboldtii cv. ＇Phraya Savet＇ and C. bicolor cv. ＇Suvarnabhum＇ using thin cell layer technique from in vitro calli were investigated. Each thin cell layer of induced callus, about 0.5 - 1 mm thick, from both caladiums was alternately laid on the top of each other for 8 layers. Subsequently, the combination of thin cell layers was cultured and the regenerated plantlets were grown in glasshouse conditions. From 3 morphological groups （leaf pattern, leaf color and petiole）, the regenerated caladium plants were found dissimilarly to both original caladiums at 85 percent with 8 types of different characters. Somatic hybridization between C. humboldtii cv. ＇Phraya Savet＇ and C. bicolor cv. ＇Suvarnabhum＇ gave rise to a number of most hybrids with all conserving C. bicolor characters.展开更多
以彩叶芋叶柄薄层组织为外植体,接种在附加2.0 mg,L BA 和0.05～0.1 mg/L NAA 的 MS 培养基上,诱导愈伤组织和胚状体的发生。培养4周后,外植体形成瘤状愈伤组织。培养8周后,从愈伤组织分化出胚状体,胚状体进一步发育成正常植株。经12周...以彩叶芋叶柄薄层组织为外植体,接种在附加2.0 mg,L BA 和0.05～0.1 mg/L NAA 的 MS 培养基上,诱导愈伤组织和胚状体的发生。培养4周后,外植体形成瘤状愈伤组织。培养8周后,从愈伤组织分化出胚状体,胚状体进一步发育成正常植株。经12周培养可再生出大量植株。组织学观察表明,其胚状体的发育和结构与典型单子叶植物合子胚的特点很相似。从一至几个细胞原胚、球形胚,至逐渐发育成棒状时,便开始胚芽、胚根、胚轴和子叶的分化,即形成完整的胚状体。胚状体的发育不同步,因而在同一块愈伤组织上可见到不同发育时期的胚状体。愈伤组织内部产生胚状体多于表面。展开更多
文摘Variations in Caladium humboldtii cv. ＇Phraya Savet＇ from in vitro culture were observed. Callus and small shoots were induced from unexpanded leaf segments cultured on modified MS medium supplemented with 2.69 p.M l-Naphthalene acetic acid （NAA） and 17.76 μM N6-Benzyladenine （BA）. Shoots were transferred onto modified MS medium supplemented with 8.88 μM BA for shoot multiplication. Subsequently, roots were induced on MS without growth regulator. The regenerated plantlets were vigorously grown in glasshouse conditions. From 4 morphological groups （leaf color ratio, leaf color, petiole and leaf pattern）, the regenerated ＇Phraya Savet＇ caladium plants were divided into 6 types. The occurrence of variants was 52%. Most of the mutated plants were observed from only leaf color ratio （green ： white） and leaf shape. The most significance for commercial value from mutated clones was round leaf obtaining 20%. Characteristics of new clones from somatic hybridization between C humboldtii cv. ＇Phraya Savet＇ and C. bicolor cv. ＇Suvarnabhum＇ using thin cell layer technique from in vitro calli were investigated. Each thin cell layer of induced callus, about 0.5 - 1 mm thick, from both caladiums was alternately laid on the top of each other for 8 layers. Subsequently, the combination of thin cell layers was cultured and the regenerated plantlets were grown in glasshouse conditions. From 3 morphological groups （leaf pattern, leaf color and petiole）, the regenerated caladium plants were found dissimilarly to both original caladiums at 85 percent with 8 types of different characters. Somatic hybridization between C. humboldtii cv. ＇Phraya Savet＇ and C. bicolor cv. ＇Suvarnabhum＇ gave rise to a number of most hybrids with all conserving C. bicolor characters.
文摘以彩叶芋叶柄薄层组织为外植体,接种在附加2.0 mg,L BA 和0.05～0.1 mg/L NAA 的 MS 培养基上,诱导愈伤组织和胚状体的发生。培养4周后,外植体形成瘤状愈伤组织。培养8周后,从愈伤组织分化出胚状体,胚状体进一步发育成正常植株。经12周培养可再生出大量植株。组织学观察表明,其胚状体的发育和结构与典型单子叶植物合子胚的特点很相似。从一至几个细胞原胚、球形胚,至逐渐发育成棒状时,便开始胚芽、胚根、胚轴和子叶的分化,即形成完整的胚状体。胚状体的发育不同步,因而在同一块愈伤组织上可见到不同发育时期的胚状体。愈伤组织内部产生胚状体多于表面。
