AIM:To measure the bone mineral density and the relative effective factors of osteoporosis in the different age groups, so as to study the relationship between both, and will offer the theoretical foundation on the pr...AIM:To measure the bone mineral density and the relative effective factors of osteoporosis in the different age groups, so as to study the relationship between both, and will offer the theoretical foundation on the prevention and treatment of osteoporosis in the old men.METHODS:Forearm bone mineral density were measured by dual energy X ray absorptiometry (DEXA); By using full automatic biochemical analytic instrument to measure serum calcium (Ca), phosphorus (P); By using radio immunoassay to measure serum parathormone(PTH),Calcitonin(CT),1,25(OH)2 D3,25(OH)D3, interleukin 6(IL 6).Ninety seven old men were divided into osteoporosis group and non osteoporosis group, were compared with normal group of men under sixty years old.RESULTS:Some results such as bone mineral density, CT,1,25(OH)2D3 and 25(OH)D3 showed decrease , while some results such as serum PTH and IL 6 showed increase with aging.The former in osteoporosis group were also significantly lower than those in non osteoporosis group (P< 0.05). The latter in osteoporosis group were also significantly higher than those in non osteoporosis group(P< 0.05-0.001).CONCLUSION:The change of the above relative effective factors lead to the following result.Bone resorption is increasing and bone formation is decreasing.These cause bone loss,at last, osteoporosis comes up.展开更多
目的:观察骨化三醇对糖尿病肾病(diabetic nephropathy,DN)大鼠肾组织中肾素水平,肾小球基底膜乙酰肝素酶(Heparanases,HPA)和足细胞podocin蛋白及m RNA表达的影响,并探讨其可能机制。方法:采用链尿佐菌素(streptozotocin,STZ)腹腔注射...目的:观察骨化三醇对糖尿病肾病(diabetic nephropathy,DN)大鼠肾组织中肾素水平,肾小球基底膜乙酰肝素酶(Heparanases,HPA)和足细胞podocin蛋白及m RNA表达的影响,并探讨其可能机制。方法:采用链尿佐菌素(streptozotocin,STZ)腹腔注射的方法构建DN大鼠动物模型,将造模成功的大鼠随机分为骨化三醇组(T组)、DN组(D组),并设置健康对照组(N组)。T组给予0.03μg·kg-1·d-1的骨化三醇灌胃;D组与N组给予等量花生油灌胃。12周后检测24小时尿蛋白定量及血生化指标,通过ELISA法检测肾组织中肾素和血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)浓度变化,免疫组化法和RT-PCR检测HPA、podocin蛋白及m RNA的表达。结果:与N组相比,T组和D组24小时尿蛋白定量、血清肌酐、肾素及AngⅡ水平较高,D组显著高于T组,差异均有统计学意义(P<0.05)。与N组相比,T组和D组HPA m RNA及蛋白表达较高,D组显著高于T组;T组和D组podocin m RNA及蛋白表达较低,D组显著低于T组,差异均有统计学意义(P<0.05)。肾素和HPA蛋白表达存在正相关趋势(r=0.79,P<0.05);和podocin蛋白表达存在负相关趋势(r=-0.65,P<0.05)。结论:骨化三醇可显著减少DN大鼠早期蛋白尿,此作用可能通过抑制肾素,下调DN肾小球基底膜HPA、上调足细胞podocin蛋白的表达实现。展开更多
文摘AIM:To measure the bone mineral density and the relative effective factors of osteoporosis in the different age groups, so as to study the relationship between both, and will offer the theoretical foundation on the prevention and treatment of osteoporosis in the old men.METHODS:Forearm bone mineral density were measured by dual energy X ray absorptiometry (DEXA); By using full automatic biochemical analytic instrument to measure serum calcium (Ca), phosphorus (P); By using radio immunoassay to measure serum parathormone(PTH),Calcitonin(CT),1,25(OH)2 D3,25(OH)D3, interleukin 6(IL 6).Ninety seven old men were divided into osteoporosis group and non osteoporosis group, were compared with normal group of men under sixty years old.RESULTS:Some results such as bone mineral density, CT,1,25(OH)2D3 and 25(OH)D3 showed decrease , while some results such as serum PTH and IL 6 showed increase with aging.The former in osteoporosis group were also significantly lower than those in non osteoporosis group (P< 0.05). The latter in osteoporosis group were also significantly higher than those in non osteoporosis group(P< 0.05-0.001).CONCLUSION:The change of the above relative effective factors lead to the following result.Bone resorption is increasing and bone formation is decreasing.These cause bone loss,at last, osteoporosis comes up.
文摘目的:观察骨化三醇对糖尿病肾病(diabetic nephropathy,DN)大鼠肾组织中肾素水平,肾小球基底膜乙酰肝素酶(Heparanases,HPA)和足细胞podocin蛋白及m RNA表达的影响,并探讨其可能机制。方法:采用链尿佐菌素(streptozotocin,STZ)腹腔注射的方法构建DN大鼠动物模型,将造模成功的大鼠随机分为骨化三醇组(T组)、DN组(D组),并设置健康对照组(N组)。T组给予0.03μg·kg-1·d-1的骨化三醇灌胃;D组与N组给予等量花生油灌胃。12周后检测24小时尿蛋白定量及血生化指标,通过ELISA法检测肾组织中肾素和血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)浓度变化,免疫组化法和RT-PCR检测HPA、podocin蛋白及m RNA的表达。结果:与N组相比,T组和D组24小时尿蛋白定量、血清肌酐、肾素及AngⅡ水平较高,D组显著高于T组,差异均有统计学意义(P<0.05)。与N组相比,T组和D组HPA m RNA及蛋白表达较高,D组显著高于T组;T组和D组podocin m RNA及蛋白表达较低,D组显著低于T组,差异均有统计学意义(P<0.05)。肾素和HPA蛋白表达存在正相关趋势(r=0.79,P<0.05);和podocin蛋白表达存在负相关趋势(r=-0.65,P<0.05)。结论:骨化三醇可显著减少DN大鼠早期蛋白尿,此作用可能通过抑制肾素,下调DN肾小球基底膜HPA、上调足细胞podocin蛋白的表达实现。
