This study examined the potential antilithic effects of a traditional Chinese medicine Urtica dentata Hand （UDH） in experimental rats and screened the optimal extract of UDH as a possible therapeutic agent for kidne...This study examined the potential antilithic effects of a traditional Chinese medicine Urtica dentata Hand （UDH） in experimental rats and screened the optimal extract of UDH as a possible therapeutic agent for kidney stones. The rat model of urinary calcium oxalate stones was induced by intragastric （i.g.） administration of 2 mL of 1.25% ethylene glycol （EG） and 1% ammonium chloride （AC） for 28 days and was confirmed by Color Doppler ultrasound imaging. The rats in different experimental groups were then intragastrically given petroleum ether extract （PEE）, N-butanol extract （NBE）, aqueous extract （AqE） of UDH, Jieshitong （positive control drug）, and saline, respectively. Treatment with NBE significantly reduced the elevated levels of urinary calcium, uric acid, phosphate, as well as increased urinary output. Accordingly, the increased calcium, oxalate levels and the number of calcium oxalate crystals deposits were remarkably reverted in the renal tissue of NBE-treated rats. In addition, NBE also prevented the impairment of renal function to decrease the contents of blood urea nitrogen （BUN） and creatinine. Taken together, these data suggest that NBE of UDH has a beneficial effect on calcium oxalate urinary stones in rats by flushing the stones out and protecting renal function.展开更多
Artificial calcium oxalate films have been applied to protect sculptures,monuments,and buildings made of marble due to their potential protective capacities.Unfortunately,artificially prepared calcium oxalate films ar...Artificial calcium oxalate films have been applied to protect sculptures,monuments,and buildings made of marble due to their potential protective capacities.Unfortunately,artificially prepared calcium oxalate films are usually loose,and easy to peel off in contrast to the natural ones.In this paper,a new method was developed for the preparation of a compact and cohesive calcium oxalate film,using ethylene glycol as an additive.The morphology,elemental composition,structure,and crystal phase of the film were analyzed by SEM,EDX,IR,and XRD,and the protective effects were investigated by acid resistance test,while its adhesion to the underlying marble by the Scotch tape test.The porosity,water absorption,and color of treated marble were also assessed.The results showed that a calcium oxalate film with a compact structure,good adhesion,and a protective effect against acid rain can be successfully obtained by including ethylene glycol as an additive.With the new preparation method,a better bond between the stone and the film is obtained which contributes to solving the spalling problem of the artificial calcium oxalate films.展开更多
INTRODUCTION:Cystone is an approved Ayurvedic polyherbal proprietary medicine used in India for various urinary disorders,including urolithiasis.AIM:To evaluate the protective effect of Cystone against hyperoxaluria-i...INTRODUCTION:Cystone is an approved Ayurvedic polyherbal proprietary medicine used in India for various urinary disorders,including urolithiasis.AIM:To evaluate the protective effect of Cystone against hyperoxaluria-induced oxidative stress and calcium oxalate crystal deposition in urolithiasis.METHODS:Ethylene glycol(EG)(0.75%,V/V)in drinking water was given to rats for 28 days to induce urolithiasis with simultaneous treatment of Cystone(500 and 750 mg/kg body weight),and various urinary risk factors of urolithiasis and antioxidant markers were assessed.RESULTS:EG treatment lead to increased urine volume and lowered urinary pH,along with increased urinary excretion of oxalate,calcium and phosphate in untreated animals.These changes caused extensive calcium oxalate crystal deposition,increased lipid peroxidation and decreased activity of antioxidant enzymes(SOD,catalase and GPx)in the kidney of untreated rats.Cystone prevented these hyperoxaluric manifestations and inhibited calcium oxalate crystal deposition in treated rats at both doses.CONCLUSIONS:Cystone therapy provides protection against hyperoxaluria-induced oxidative stress and calcium oxalate crystal deposition by improving renal tissue antioxidant status and diuresis.展开更多
基金supported by a grant from the National Natural Sciences Foundation of China (No. 81073124)
文摘This study examined the potential antilithic effects of a traditional Chinese medicine Urtica dentata Hand （UDH） in experimental rats and screened the optimal extract of UDH as a possible therapeutic agent for kidney stones. The rat model of urinary calcium oxalate stones was induced by intragastric （i.g.） administration of 2 mL of 1.25% ethylene glycol （EG） and 1% ammonium chloride （AC） for 28 days and was confirmed by Color Doppler ultrasound imaging. The rats in different experimental groups were then intragastrically given petroleum ether extract （PEE）, N-butanol extract （NBE）, aqueous extract （AqE） of UDH, Jieshitong （positive control drug）, and saline, respectively. Treatment with NBE significantly reduced the elevated levels of urinary calcium, uric acid, phosphate, as well as increased urinary output. Accordingly, the increased calcium, oxalate levels and the number of calcium oxalate crystals deposits were remarkably reverted in the renal tissue of NBE-treated rats. In addition, NBE also prevented the impairment of renal function to decrease the contents of blood urea nitrogen （BUN） and creatinine. Taken together, these data suggest that NBE of UDH has a beneficial effect on calcium oxalate urinary stones in rats by flushing the stones out and protecting renal function.
基金supported by the National Natural Science Foundation of China(Grant Nos.B050121975202,52108031)the Natural Science Foundation of Shaanxi Province(Grant No.2023-JC-YB-097)the 111Project(Grant No.D18004)。
文摘Artificial calcium oxalate films have been applied to protect sculptures,monuments,and buildings made of marble due to their potential protective capacities.Unfortunately,artificially prepared calcium oxalate films are usually loose,and easy to peel off in contrast to the natural ones.In this paper,a new method was developed for the preparation of a compact and cohesive calcium oxalate film,using ethylene glycol as an additive.The morphology,elemental composition,structure,and crystal phase of the film were analyzed by SEM,EDX,IR,and XRD,and the protective effects were investigated by acid resistance test,while its adhesion to the underlying marble by the Scotch tape test.The porosity,water absorption,and color of treated marble were also assessed.The results showed that a calcium oxalate film with a compact structure,good adhesion,and a protective effect against acid rain can be successfully obtained by including ethylene glycol as an additive.With the new preparation method,a better bond between the stone and the film is obtained which contributes to solving the spalling problem of the artificial calcium oxalate films.
文摘INTRODUCTION:Cystone is an approved Ayurvedic polyherbal proprietary medicine used in India for various urinary disorders,including urolithiasis.AIM:To evaluate the protective effect of Cystone against hyperoxaluria-induced oxidative stress and calcium oxalate crystal deposition in urolithiasis.METHODS:Ethylene glycol(EG)(0.75%,V/V)in drinking water was given to rats for 28 days to induce urolithiasis with simultaneous treatment of Cystone(500 and 750 mg/kg body weight),and various urinary risk factors of urolithiasis and antioxidant markers were assessed.RESULTS:EG treatment lead to increased urine volume and lowered urinary pH,along with increased urinary excretion of oxalate,calcium and phosphate in untreated animals.These changes caused extensive calcium oxalate crystal deposition,increased lipid peroxidation and decreased activity of antioxidant enzymes(SOD,catalase and GPx)in the kidney of untreated rats.Cystone prevented these hyperoxaluric manifestations and inhibited calcium oxalate crystal deposition in treated rats at both doses.CONCLUSIONS:Cystone therapy provides protection against hyperoxaluria-induced oxidative stress and calcium oxalate crystal deposition by improving renal tissue antioxidant status and diuresis.
