Calf spleen extract(CSE) has been clinically used as an adjuvant agent in malignant tumor therapy.It can improve the physical status of patients.However,its mechanism of action remains relatively unclear.In this study...Calf spleen extract(CSE) has been clinically used as an adjuvant agent in malignant tumor therapy.It can improve the physical status of patients.However,its mechanism of action remains relatively unclear.In this study,we investigated the effect of CSE on leucopenia in mice and on promyelocytic leukemia cells(HL60).CSE in 10 mL/kg(2.5 mg bioactive polypeptides and 190μg ribose/mL) significantly increased leukocyte numbers in leucopenia mice.The effect on neutrophil numbers,among all leukocytes,was the most evident.CSE stimulated the proliferation of bone marrow cells in vitro and decreased the GM-CSF level in serum.In addition,CSE significantly reduced the viability of HL60 cells,decreased the production of lactate and adenosine triphosphate(ATP) of these cells.CSE induced the apoptosis and S-phase arrest in HL60 cells.In conclusion,CSE can enhance leukocyte numbers,which may be attributed to its direct stimulatory effect on bone marrow cells.CSE is an inhibitor of promyelocytic leukemia cell viability,which may be attributed to the induction of the apoptosis,the arrest of cell cycle and the inhibition of the glycolysis of cells.展开更多
Calf spleen extract （CSE） has been clinically used as an adjuvant agent in malignant tumor therapy. It can improve the physical status of patients. However, its mechanism of action remains relatively unclear. In thi...Calf spleen extract （CSE） has been clinically used as an adjuvant agent in malignant tumor therapy. It can improve the physical status of patients. However, its mechanism of action remains relatively unclear. In this study, we investigated the effect of CSE on leucopenia in mice and on promyelocytic leukemia cells （HL60）. CSE in 10 mL/kg （2.5 mg bioactive polypeptides and 190 pg ribose/mL） significantly increased leukocyte numbers in leucopenia mice. The effect on neutrophil numbers, among all leukocytes, was the most evident. CSE stimulated the proliferation of bone marrow cells in vitro and decreased the GM-CSF level in serum. In addition, CSE significantly reduced the viability of HL60 cells, decreased the production of lactate and adenosine triphosphate （ATP） of these cells. CSE induced the apoptosis and S-phase arrest in HL60 cells. In conclusion, CSE can enhance leukocyte numbers, which may be attributed to its direct stimulatory effect on bone marrow cells. CSE is an inhibitor ofpromyelocytic leukemia cell viability, which may be attributed to the induction of the apoptosis, the arrest of cell cycle and the inhibition of the glycolysis of cells.展开更多
目的探讨小牛脾提取物对卵巢上皮性恶性肿瘤术后化疗患者的疗效及对血清中金属蛋白酶(MMP)-2和糖蛋白抗原125(CA125)的影响。方法将88例卵巢上皮性恶性肿瘤患者随机分为对照组44例和治疗组44例。对照组采取手术联合术后化疗。治疗组在...目的探讨小牛脾提取物对卵巢上皮性恶性肿瘤术后化疗患者的疗效及对血清中金属蛋白酶(MMP)-2和糖蛋白抗原125(CA125)的影响。方法将88例卵巢上皮性恶性肿瘤患者随机分为对照组44例和治疗组44例。对照组采取手术联合术后化疗。治疗组在对照组基础上予小牛脾提取物注射液,1次/d,10 m L/次,连续8周。比较两组生活质量、不良反应及血清中MMP-2及CA125水平。结果治疗组化疗后8周疼痛、恶心呕吐、疲倦评分明显低于对照组,躯体功能、角色功能、认知功能、情绪功能及社会功能评分显著高于对照组(P<0.01)。治疗组白细胞减少、恶心呕吐及肝肾功能损伤发生率显著低于对照组(P<0.05),两组神经毒性发生率比较无统计学意义(P>0.05)。化疗后8周,治疗组血清中MMP-2和CA125水平明显低于对照组(P<0.01)。结论小牛脾提取物可明显改善恶性卵巢肿瘤术后化疗患者的生活质量,降低不良反应,抑制患者血清中MMP-2和CA125水平可能与其疗效有关。展开更多
文摘Calf spleen extract(CSE) has been clinically used as an adjuvant agent in malignant tumor therapy.It can improve the physical status of patients.However,its mechanism of action remains relatively unclear.In this study,we investigated the effect of CSE on leucopenia in mice and on promyelocytic leukemia cells(HL60).CSE in 10 mL/kg(2.5 mg bioactive polypeptides and 190μg ribose/mL) significantly increased leukocyte numbers in leucopenia mice.The effect on neutrophil numbers,among all leukocytes,was the most evident.CSE stimulated the proliferation of bone marrow cells in vitro and decreased the GM-CSF level in serum.In addition,CSE significantly reduced the viability of HL60 cells,decreased the production of lactate and adenosine triphosphate(ATP) of these cells.CSE induced the apoptosis and S-phase arrest in HL60 cells.In conclusion,CSE can enhance leukocyte numbers,which may be attributed to its direct stimulatory effect on bone marrow cells.CSE is an inhibitor of promyelocytic leukemia cell viability,which may be attributed to the induction of the apoptosis,the arrest of cell cycle and the inhibition of the glycolysis of cells.
文摘Calf spleen extract （CSE） has been clinically used as an adjuvant agent in malignant tumor therapy. It can improve the physical status of patients. However, its mechanism of action remains relatively unclear. In this study, we investigated the effect of CSE on leucopenia in mice and on promyelocytic leukemia cells （HL60）. CSE in 10 mL/kg （2.5 mg bioactive polypeptides and 190 pg ribose/mL） significantly increased leukocyte numbers in leucopenia mice. The effect on neutrophil numbers, among all leukocytes, was the most evident. CSE stimulated the proliferation of bone marrow cells in vitro and decreased the GM-CSF level in serum. In addition, CSE significantly reduced the viability of HL60 cells, decreased the production of lactate and adenosine triphosphate （ATP） of these cells. CSE induced the apoptosis and S-phase arrest in HL60 cells. In conclusion, CSE can enhance leukocyte numbers, which may be attributed to its direct stimulatory effect on bone marrow cells. CSE is an inhibitor ofpromyelocytic leukemia cell viability, which may be attributed to the induction of the apoptosis, the arrest of cell cycle and the inhibition of the glycolysis of cells.
文摘目的探讨小牛脾提取物对卵巢上皮性恶性肿瘤术后化疗患者的疗效及对血清中金属蛋白酶(MMP)-2和糖蛋白抗原125(CA125)的影响。方法将88例卵巢上皮性恶性肿瘤患者随机分为对照组44例和治疗组44例。对照组采取手术联合术后化疗。治疗组在对照组基础上予小牛脾提取物注射液,1次/d,10 m L/次,连续8周。比较两组生活质量、不良反应及血清中MMP-2及CA125水平。结果治疗组化疗后8周疼痛、恶心呕吐、疲倦评分明显低于对照组,躯体功能、角色功能、认知功能、情绪功能及社会功能评分显著高于对照组(P<0.01)。治疗组白细胞减少、恶心呕吐及肝肾功能损伤发生率显著低于对照组(P<0.05),两组神经毒性发生率比较无统计学意义(P>0.05)。化疗后8周,治疗组血清中MMP-2和CA125水平明显低于对照组(P<0.01)。结论小牛脾提取物可明显改善恶性卵巢肿瘤术后化疗患者的生活质量,降低不良反应,抑制患者血清中MMP-2和CA125水平可能与其疗效有关。
