The effects of auxins and media on callus induction from the mature and immature embryos of Chinese spring wheat （Triticum aestivum L.） varieties were investigated. It was found that genotype, medium, auxin source a...The effects of auxins and media on callus induction from the mature and immature embryos of Chinese spring wheat （Triticum aestivum L.） varieties were investigated. It was found that genotype, medium, auxin source and concentration had the significant effects on the induction of embryogenic callus, explants germination and the increment of callus fresh weight. For immature embryos cultured on MS medium, 2 mg L^-1 of 2, 4-D was optimal, and the highest frequency of embryogenic callus （33.50%） was observed. For the mature embryos on N6 medium, 4 mg L^-1 of 2, 4-D was optimal. The frequency of embryogenic callus and increment of callus fresh weight on 2, 4, 5-T media were higher than those on 2, 4-D media, and in the presence of 2, 4, 5-T the precocious germination of explants for all genotypes were significantly suppressed. These results indicated that 2, 4, 5-T was superior to 2, 4-D and NAA in the culture of immature embryos. This is the first report about the effect of 2, 4, 5-T and NAA on wheat tissue culture, particularly in comparison with 2, 4-D in detail.展开更多
为了加快油茶品种改良,提高油茶花药愈伤组织的诱导效率,在低温预处理(4℃)的基础上,以M S为基本培养基,研究了不同浓度的2,4-D、NAA及2,4-D、6-BA配比对普通油茶花药愈伤组织诱导效率的影响。结果表明:2,4-D、NAA诱导普通油茶愈伤组织...为了加快油茶品种改良,提高油茶花药愈伤组织的诱导效率,在低温预处理(4℃)的基础上,以M S为基本培养基,研究了不同浓度的2,4-D、NAA及2,4-D、6-BA配比对普通油茶花药愈伤组织诱导效率的影响。结果表明:2,4-D、NAA诱导普通油茶愈伤组织的较优组合为2,4-D 0.6 m g/L,NAA 0.05或0.5 m g/L,愈伤组织诱导率分别达到35.56%和24.65%,在油茶花药愈伤组织诱导过程中,2,4-D有利于愈伤组织的形成,是最重要的因素;2,4-D、6-BA的混合使用更有利于愈伤组织的发生,并且两种激素适当的配比是提高愈伤组织诱导率的重要因素,0.5 m g/L的2,4-D与0.2 m g/L 6-BA组合是实验得出的较优水平,愈伤组织诱导率达到42.74%。展开更多
基金the National High Tech R&D Program of China(863 Program,2003AA207100)the Foundation for the Author of National Excellent Doctoral Dissertation of P R China(200357) the Program forChangjiang Scholars and Innovative Research Team inUniversity(IRT0453).
文摘The effects of auxins and media on callus induction from the mature and immature embryos of Chinese spring wheat （Triticum aestivum L.） varieties were investigated. It was found that genotype, medium, auxin source and concentration had the significant effects on the induction of embryogenic callus, explants germination and the increment of callus fresh weight. For immature embryos cultured on MS medium, 2 mg L^-1 of 2, 4-D was optimal, and the highest frequency of embryogenic callus （33.50%） was observed. For the mature embryos on N6 medium, 4 mg L^-1 of 2, 4-D was optimal. The frequency of embryogenic callus and increment of callus fresh weight on 2, 4, 5-T media were higher than those on 2, 4-D media, and in the presence of 2, 4, 5-T the precocious germination of explants for all genotypes were significantly suppressed. These results indicated that 2, 4, 5-T was superior to 2, 4-D and NAA in the culture of immature embryos. This is the first report about the effect of 2, 4, 5-T and NAA on wheat tissue culture, particularly in comparison with 2, 4-D in detail.
文摘为了加快油茶品种改良,提高油茶花药愈伤组织的诱导效率,在低温预处理(4℃)的基础上,以M S为基本培养基,研究了不同浓度的2,4-D、NAA及2,4-D、6-BA配比对普通油茶花药愈伤组织诱导效率的影响。结果表明:2,4-D、NAA诱导普通油茶愈伤组织的较优组合为2,4-D 0.6 m g/L,NAA 0.05或0.5 m g/L,愈伤组织诱导率分别达到35.56%和24.65%,在油茶花药愈伤组织诱导过程中,2,4-D有利于愈伤组织的形成,是最重要的因素;2,4-D、6-BA的混合使用更有利于愈伤组织的发生,并且两种激素适当的配比是提高愈伤组织诱导率的重要因素,0.5 m g/L的2,4-D与0.2 m g/L 6-BA组合是实验得出的较优水平,愈伤组织诱导率达到42.74%。
