建立了超高效液相色谱-电喷雾串联三重四级杆质谱法(UPLC-ESI-MS)同时测定贞芪扶正胶囊中红景天苷、毛蕊异黄酮-7-O-β-D-葡萄糖苷和黄芪甲苷含量的方法.采用UPLC-ESI-MS联用法,乙腈和0.3%甲酸水溶液不同比例梯度洗脱,流速0.3 m L/min,1...建立了超高效液相色谱-电喷雾串联三重四级杆质谱法(UPLC-ESI-MS)同时测定贞芪扶正胶囊中红景天苷、毛蕊异黄酮-7-O-β-D-葡萄糖苷和黄芪甲苷含量的方法.采用UPLC-ESI-MS联用法,乙腈和0.3%甲酸水溶液不同比例梯度洗脱,流速0.3 m L/min,12 min内可完成测试,且线性关系良好(r2>0.999).方法简便、准确、可靠、重复性好,可用于贞芪扶正制剂的质量控制.展开更多
Aim To determine calycosin-7-O-β-D-glucoside,astragaloside IV and formononetin in Radix Astragali and other relative samples by HPLC-MS.Methods HPLC was carried out with Agilent 1100LC/MSD,equipped with Agilent Zorba...Aim To determine calycosin-7-O-β-D-glucoside,astragaloside IV and formononetin in Radix Astragali and other relative samples by HPLC-MS.Methods HPLC was carried out with Agilent 1100LC/MSD,equipped with Agilent Zorbax SB C18 column(250 mm×4.6 mm ID,5 μm) and mass spectrum detector.The mobile phase(CH3CN-H2O) was eluted in gradient mode.Results The calibration curves of calycosin-7-O-β-D-glucoside,astragaloside IV and formononetin were linear in the range of 0.031.21 μg·mL-1,0.35-13.86 μg·mL-1 and 0.38-15.22 μg·mL-1,respectively.These recoveries of samples were from 95% to 105% with RSD less than 1.5%.Conclusion The method was employed to analyse 25 samples of Radix Astragali and other relative samples,including Radix Astragali slice,Radix Astragali Preparata,Hedysarum polybotrys Hand.-Mazz,Astragalus ernestii Comb.The contents of three constituents vary greatly because of the species, place of collection and season of harvesting.This method could apply to evaluate the quality of Radix Astragali and it is simple,sensitive and reliable.展开更多
文摘建立了超高效液相色谱-电喷雾串联三重四级杆质谱法(UPLC-ESI-MS)同时测定贞芪扶正胶囊中红景天苷、毛蕊异黄酮-7-O-β-D-葡萄糖苷和黄芪甲苷含量的方法.采用UPLC-ESI-MS联用法,乙腈和0.3%甲酸水溶液不同比例梯度洗脱,流速0.3 m L/min,12 min内可完成测试,且线性关系良好(r2>0.999).方法简便、准确、可靠、重复性好,可用于贞芪扶正制剂的质量控制.
文摘Aim To determine calycosin-7-O-β-D-glucoside,astragaloside IV and formononetin in Radix Astragali and other relative samples by HPLC-MS.Methods HPLC was carried out with Agilent 1100LC/MSD,equipped with Agilent Zorbax SB C18 column(250 mm×4.6 mm ID,5 μm) and mass spectrum detector.The mobile phase(CH3CN-H2O) was eluted in gradient mode.Results The calibration curves of calycosin-7-O-β-D-glucoside,astragaloside IV and formononetin were linear in the range of 0.031.21 μg·mL-1,0.35-13.86 μg·mL-1 and 0.38-15.22 μg·mL-1,respectively.These recoveries of samples were from 95% to 105% with RSD less than 1.5%.Conclusion The method was employed to analyse 25 samples of Radix Astragali and other relative samples,including Radix Astragali slice,Radix Astragali Preparata,Hedysarum polybotrys Hand.-Mazz,Astragalus ernestii Comb.The contents of three constituents vary greatly because of the species, place of collection and season of harvesting.This method could apply to evaluate the quality of Radix Astragali and it is simple,sensitive and reliable.