The isoflavone compound Calycosin-7-O-β-D-glucoside (CG) is an active monomer component extracted from the dry roots of the leguminous plant Astragalus mongolicus and Astragalus. It is also one of the main active ing...The isoflavone compound Calycosin-7-O-β-D-glucoside (CG) is an active monomer component extracted from the dry roots of the leguminous plant Astragalus mongolicus and Astragalus. It is also one of the main active ingredients in the Astragalus that is a commonly used traditional herb. CG has obvious effects of anti-oxidation, anti-virus, inhibition of melanin formation, and immunosuppression. With the advancement of modern technology, it has become a pivotal subject that the adjuvant therapy or even substitute for the synthetic drug of monomer of Chinese herb in medical field. In recent years, with the deepening of research on the mechanism of action of CG, which has been found that its pharmacological effects are very extensive, such as the anti-tumor effect and the effect on cerebrovascular diseases of CG. This review summarizes the pharmacological effects and the latest research progress of CG.展开更多
The iso fl avone calycosin-7-O-β-D-glucopyranoside(CG) is a principal constituent of Astragalus membranaceus(AR) and has been reported to inhibit osteoclast development in vitro and bone loss in vivo. The aim of this...The iso fl avone calycosin-7-O-β-D-glucopyranoside(CG) is a principal constituent of Astragalus membranaceus(AR) and has been reported to inhibit osteoclast development in vitro and bone loss in vivo. The aim of this study was to investigate the osteogenic effects of CG and its underlying mechanism in ST2 cells. The results show that exposure of cells to CG in osteogenic differentiation medium increases ALP activity, osteocalcin(Ocal) m RNA expression and the osteoblastic mineralization process. Mechanistically, CG treatment increased the expression of bone morphogenetic protein 2(BMP-2), p-Smad 1/5/8, β-catenin and Runx2, all of which are regulators of the BMP- or wingless-type MMTV integration site family(WNT)/β-catenin-signaling pathways. Moreover, the osteogenic effects of CG were inhibited by Noggin and DKK-1 which are classical inhibitors of the BMP and WNT/β-catenin-signaling pathways, respectively. Taken together, the results indicate that CG promotes the osteoblastic differentiation of ST2 cells through regulating the BMP/WNT signaling pathways. On this basis, CG may be a useful lead compound for improving the treatment of bone-decreasing diseases and enhancing bone regeneration.展开更多
A new isoflavone glucoside, 3'-methoxy-5'-hydroxy-isoflavone-7-O-beta-D-glucoside, was isolated from the roots of Astragalus membranaceus and its structure was elucidated by spectroscopic data.
基金General Project Foundation of Guangdong Traditional Chinese Medicine (20191192)
文摘The isoflavone compound Calycosin-7-O-β-D-glucoside (CG) is an active monomer component extracted from the dry roots of the leguminous plant Astragalus mongolicus and Astragalus. It is also one of the main active ingredients in the Astragalus that is a commonly used traditional herb. CG has obvious effects of anti-oxidation, anti-virus, inhibition of melanin formation, and immunosuppression. With the advancement of modern technology, it has become a pivotal subject that the adjuvant therapy or even substitute for the synthetic drug of monomer of Chinese herb in medical field. In recent years, with the deepening of research on the mechanism of action of CG, which has been found that its pharmacological effects are very extensive, such as the anti-tumor effect and the effect on cerebrovascular diseases of CG. This review summarizes the pharmacological effects and the latest research progress of CG.
基金supported by the National Natural Science Foundation of China, China (No. 31400304)the Natural Science Foundation of Hubei Province, China (No. 2012FFB00303)+1 种基金the Youth & Middle-aged Talent Project of Hubei Province (No. Q20111005)the Science and Technology Program of Shandong Province (No. J12LL07)
文摘The iso fl avone calycosin-7-O-β-D-glucopyranoside(CG) is a principal constituent of Astragalus membranaceus(AR) and has been reported to inhibit osteoclast development in vitro and bone loss in vivo. The aim of this study was to investigate the osteogenic effects of CG and its underlying mechanism in ST2 cells. The results show that exposure of cells to CG in osteogenic differentiation medium increases ALP activity, osteocalcin(Ocal) m RNA expression and the osteoblastic mineralization process. Mechanistically, CG treatment increased the expression of bone morphogenetic protein 2(BMP-2), p-Smad 1/5/8, β-catenin and Runx2, all of which are regulators of the BMP- or wingless-type MMTV integration site family(WNT)/β-catenin-signaling pathways. Moreover, the osteogenic effects of CG were inhibited by Noggin and DKK-1 which are classical inhibitors of the BMP and WNT/β-catenin-signaling pathways, respectively. Taken together, the results indicate that CG promotes the osteoblastic differentiation of ST2 cells through regulating the BMP/WNT signaling pathways. On this basis, CG may be a useful lead compound for improving the treatment of bone-decreasing diseases and enhancing bone regeneration.
文摘A new isoflavone glucoside, 3'-methoxy-5'-hydroxy-isoflavone-7-O-beta-D-glucoside, was isolated from the roots of Astragalus membranaceus and its structure was elucidated by spectroscopic data.
