Background:Emerging data suggested that liquid biopsy such as detection of circulating tumor cells(CTCs)and cell-free tumor DNA analysis augments the management of patients with urothelial cancer(UC).We presented our ...Background:Emerging data suggested that liquid biopsy such as detection of circulating tumor cells(CTCs)and cell-free tumor DNA analysis augments the management of patients with urothelial cancer(UC).We presented our pilot experience of liquid biopsy using the Ion Torrent platform to detect CTCs and genomic alterations in UC.Materials and methods:Blood or urine samples from 16 patients were subjected to CTC and plasma/urine cell-free tumor DNA isolation for next generation sequencing(NGS)using the Ion S5 system to detect mutations among 50 oncogenes on the Ion AmpliSeq Cancer Hotspot Panel.Results:The Ion Torrent platform detected a higher number of CTCs than those in previous studies using the CellSearch^(TM)system.Overall,mutations were detected in 13/16(81.3%)patients with a median number of 18(range 12-25).NGS isolated 17 hotspot mutations from 11 genes and 41 novel genomic alterations from 24 genes,some of which are supposed to be clinically actionable.Conclusions:The Ion Torrent platform efficiently detected CTCs compared with previous reports.NGS with the present system also allowed for detection of gene alterations which are likely to be therapeutic targets and provided an attractive tool to guide personalized therapy for patients with advanced UC.展开更多
Induced pluripotent stem （iPS） cells are a recent development which has brought a promise of great therapeutic values. The previous technique of somatic ceil nuclear transfer （SCNT） has been ineffective in humans....Induced pluripotent stem （iPS） cells are a recent development which has brought a promise of great therapeutic values. The previous technique of somatic ceil nuclear transfer （SCNT） has been ineffective in humans. Recent discoveries show that human fibroblasls can be reprogrammed by a transient over expression of a small number of genes; they can undergo induced pluripotency, iPS were first produced in 2006. By 2008, work was underway to remove the potential oncogenes from their structure. In 2009, protein iPS （piPS） cells were discovered. Surface markers and reporter genes play an important role in stem cell research. Clinical applications include generation of self renewing stem cells, tissue replacement and many more. Stem cell therapy has the ability to dramatically change the treatment of human diseases.展开更多
文摘Background:Emerging data suggested that liquid biopsy such as detection of circulating tumor cells(CTCs)and cell-free tumor DNA analysis augments the management of patients with urothelial cancer(UC).We presented our pilot experience of liquid biopsy using the Ion Torrent platform to detect CTCs and genomic alterations in UC.Materials and methods:Blood or urine samples from 16 patients were subjected to CTC and plasma/urine cell-free tumor DNA isolation for next generation sequencing(NGS)using the Ion S5 system to detect mutations among 50 oncogenes on the Ion AmpliSeq Cancer Hotspot Panel.Results:The Ion Torrent platform detected a higher number of CTCs than those in previous studies using the CellSearch^(TM)system.Overall,mutations were detected in 13/16(81.3%)patients with a median number of 18(range 12-25).NGS isolated 17 hotspot mutations from 11 genes and 41 novel genomic alterations from 24 genes,some of which are supposed to be clinically actionable.Conclusions:The Ion Torrent platform efficiently detected CTCs compared with previous reports.NGS with the present system also allowed for detection of gene alterations which are likely to be therapeutic targets and provided an attractive tool to guide personalized therapy for patients with advanced UC.
文摘Induced pluripotent stem （iPS） cells are a recent development which has brought a promise of great therapeutic values. The previous technique of somatic ceil nuclear transfer （SCNT） has been ineffective in humans. Recent discoveries show that human fibroblasls can be reprogrammed by a transient over expression of a small number of genes; they can undergo induced pluripotency, iPS were first produced in 2006. By 2008, work was underway to remove the potential oncogenes from their structure. In 2009, protein iPS （piPS） cells were discovered. Surface markers and reporter genes play an important role in stem cell research. Clinical applications include generation of self renewing stem cells, tissue replacement and many more. Stem cell therapy has the ability to dramatically change the treatment of human diseases.
