A new cyclodipeptide, cyclo(phenylalanyl-N-methyltyrosyl) 1, was isolated from the mycelial solid cultures of Geotrichum candidum. Its structure was elucidated by spectroscopic methods. The antifungal activity of the ...A new cyclodipeptide, cyclo(phenylalanyl-N-methyltyrosyl) 1, was isolated from the mycelial solid cultures of Geotrichum candidum. Its structure was elucidated by spectroscopic methods. The antifungal activity of the new compound against Peronophythora litchii was assessed by microplate Alamar blue assay (MABA).展开更多
Potato(Solanum tuberosum L.)is an important crop throughout the world.An uncharacterized disease has been observed on potato plants during the growing season and tubers during the storage period from Nileke County,Qit...Potato(Solanum tuberosum L.)is an important crop throughout the world.An uncharacterized disease has been observed on potato plants during the growing season and tubers during the storage period from Nileke County,Qitai County and other locations in Xinjiang,China.A particular fungus was consistently isolated from the infected potato plants and tubers.Based on its morphology,molecular characteristics,pathogenicity test and internal transcribed spacer(ITS)sequence,the pathogens was identified as Galactomyces candidum F12.Further study also showed that the hyphae and conidia of the pathogenic fungus grew faster as the temperature was 30℃,pH was 7,soluble starch was used as optimal carbon source and yeast powder as optimal nitrogen source.In addition,12-h continuous ilumination light was beneficial to the hyphal growth,while 24-h continuous ilumination was beneficial to the sporulation of the strain at 30℃.To our knowledge,this is the first report of Galactomyces candidum causing leaf wilt and postharvest tuber rot on potato in China.展开更多
Fusarium oxysporum and Geotrichum candidum, which are among important pathogens of Solanum lycopersicum L. (Tomato), are sometimes misidentified during morphological misidentification. The study was carried out to eva...Fusarium oxysporum and Geotrichum candidum, which are among important pathogens of Solanum lycopersicum L. (Tomato), are sometimes misidentified during morphological misidentification. The study was carried out to evaluate molecular diversity of F. oxysporum and G. candidum isolated from two tomato varieties obtained from Akure, Ilorin and Ibadan, Nigeria. The tomato samples were collected and brought back to the laboratory for fungal isolation. Isolation of the pathogens were done following standard procedures. DNA extraction from pure cultures of the pathogens was done at the Centre Laboratory of University of Ibadan. Genetic relationships among the organisms were also estimated by constructing a Dendrogram through UPGMA using the Mega6 Software and genetic distance was computed also using the Mega6 Software. Five strains of F. oxysporum and seven strains of G. candidum were identified. Percentage similarity of the pathogens with those in GenBank was 99.17% - 100.00% for F. oxysporum and 98.48% - 100.00% for G. candidum. The T-01 marker showed the lowest major allele frequency of 0.0833, while T-10 marker has the highest value for major allele frequency of 0.6667 and an average value of 0.3958. Evolutionary relationship showed that the two strains of G. candidum (MN650247 and MN650250) were similar. The three strains of F. oxysporum (MN650246 and MN650248, MN650245 and MN650253) were also similar. Genetic distances among pairs of the fungal strains ranged from 0.12 to 6.30 in pairwise fashion, with an average of 1.32. Evolutionary relationship or closeness among strains of a fungal species can thus be said not to depend on location.展开更多
This paper is a report of a 34-year-old man with chronic renal failure undergoing Continuous Ambulatory Peritoneal Dialysis which developed peritonitis due to Geotricum candidum. The diagnosis was established by cultu...This paper is a report of a 34-year-old man with chronic renal failure undergoing Continuous Ambulatory Peritoneal Dialysis which developed peritonitis due to Geotricum candidum. The diagnosis was established by culture of dialysis fluid. The purpose of this report is to provide data on a fungal peritonitis due to a non-common agent.展开更多
从云南昆明地区的开菲尔粒中分离得到K2、Z7-1和Z8-4菌株,3株菌发酵产物可产生愉悦的花果香味。在基因组重测序基础上,分析菌株K2、Z7-1、Z8-4与白地霉(Geotrichum candidum)LMA-244基因组相似度,DNA杂交分析相似度分别为99.50%、99.40%...从云南昆明地区的开菲尔粒中分离得到K2、Z7-1和Z8-4菌株,3株菌发酵产物可产生愉悦的花果香味。在基因组重测序基础上,分析菌株K2、Z7-1、Z8-4与白地霉(Geotrichum candidum)LMA-244基因组相似度,DNA杂交分析相似度分别为99.50%、99.40%、99.50%;平均核酸一致性分析,相似度分别为98.66%、98.65%、98.68%。结合形态特征和26S rDNA序列同源性分析,K2、Z7-1和Z8-43株菌均鉴定为白地霉。利用基因本体论、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)、直系同源簇等数据库对白地霉K2进行基因功能注释,注释基因中40.74%与细胞组成相关,47.54%与生物过程相关,11.73%与分子功能相关。KEGG代谢途径分析发现萜类合成的相关基因,白地霉K2具有合成挥发性香味成分的遗传基础。采用气相色谱-质谱联用仪对白地霉K2菌株的发酵液挥发性成分进行检测分析,共检出50种挥发性成分,其中3-蒈烯、α-蒎烯和对伞花烃呈花香,L-α-松油醇呈松木香,4-萜烯醇、(-)-4-萜品醇呈丁香味,乙酸α-松油酯呈清甜香。推测白地霉是开菲尔酸奶香味物质的重要贡献菌株。本研究为白地霉K2用于发酵乳制品及其他发酵食品提供了理论基础。展开更多
基于CiteSpace知识图谱与专利分析,探讨铁皮石斛的研究热点与发展趋势。通过系统检索中国知网(CNKI)数据库和Web of Science(WOS)核心合集数据库关于铁皮石斛的中、英文文献,运用CiteSpace 5.8.R3软件对关键词信息绘制知识图谱,进行铁...基于CiteSpace知识图谱与专利分析,探讨铁皮石斛的研究热点与发展趋势。通过系统检索中国知网(CNKI)数据库和Web of Science(WOS)核心合集数据库关于铁皮石斛的中、英文文献,运用CiteSpace 5.8.R3软件对关键词信息绘制知识图谱,进行铁皮石斛发展历程与研究热点的可视化分析,并结合专利计量学研究手段分析铁皮石斛相关专利的领域分布与发展趋势,以及铁皮石斛在食品应用开发方面的专利情况。结果表明,研究热点国内外各有侧重,国内专注于铁皮石斛多糖、保健功能、化学成分、产品开发及基因等研究,国外偏向于动物实验及安全性评价等临床实验研究,在活性成分鉴定、药理作用及作用机制等领域研究较多。专利计量分析发现铁皮石斛食品种类丰富且大多为明确保健功能的食品,共涉及9种保健功能,其中以增强免疫力为主,占比42.04%,铁皮石斛在食品领域可在不同保健功能开发,产品制备技术以及铁皮石斛花、叶上进一步进行研究。本研究为分析铁皮石斛的研究内容、判断研究热点及食品专利申请方向提供参考。展开更多
文摘A new cyclodipeptide, cyclo(phenylalanyl-N-methyltyrosyl) 1, was isolated from the mycelial solid cultures of Geotrichum candidum. Its structure was elucidated by spectroscopic methods. The antifungal activity of the new compound against Peronophythora litchii was assessed by microplate Alamar blue assay (MABA).
基金Supported by the Basic Scientific Research Fund Project of the Public Welfare Scientific Research Institute of Xinjiang Autonomous Region,China(KY2018012,KY2019020)the Open Fund of Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis,Ministry of Agriculture and Rural Affairs,China(KFJJ202006).
文摘Potato(Solanum tuberosum L.)is an important crop throughout the world.An uncharacterized disease has been observed on potato plants during the growing season and tubers during the storage period from Nileke County,Qitai County and other locations in Xinjiang,China.A particular fungus was consistently isolated from the infected potato plants and tubers.Based on its morphology,molecular characteristics,pathogenicity test and internal transcribed spacer(ITS)sequence,the pathogens was identified as Galactomyces candidum F12.Further study also showed that the hyphae and conidia of the pathogenic fungus grew faster as the temperature was 30℃,pH was 7,soluble starch was used as optimal carbon source and yeast powder as optimal nitrogen source.In addition,12-h continuous ilumination light was beneficial to the hyphal growth,while 24-h continuous ilumination was beneficial to the sporulation of the strain at 30℃.To our knowledge,this is the first report of Galactomyces candidum causing leaf wilt and postharvest tuber rot on potato in China.
文摘Fusarium oxysporum and Geotrichum candidum, which are among important pathogens of Solanum lycopersicum L. (Tomato), are sometimes misidentified during morphological misidentification. The study was carried out to evaluate molecular diversity of F. oxysporum and G. candidum isolated from two tomato varieties obtained from Akure, Ilorin and Ibadan, Nigeria. The tomato samples were collected and brought back to the laboratory for fungal isolation. Isolation of the pathogens were done following standard procedures. DNA extraction from pure cultures of the pathogens was done at the Centre Laboratory of University of Ibadan. Genetic relationships among the organisms were also estimated by constructing a Dendrogram through UPGMA using the Mega6 Software and genetic distance was computed also using the Mega6 Software. Five strains of F. oxysporum and seven strains of G. candidum were identified. Percentage similarity of the pathogens with those in GenBank was 99.17% - 100.00% for F. oxysporum and 98.48% - 100.00% for G. candidum. The T-01 marker showed the lowest major allele frequency of 0.0833, while T-10 marker has the highest value for major allele frequency of 0.6667 and an average value of 0.3958. Evolutionary relationship showed that the two strains of G. candidum (MN650247 and MN650250) were similar. The three strains of F. oxysporum (MN650246 and MN650248, MN650245 and MN650253) were also similar. Genetic distances among pairs of the fungal strains ranged from 0.12 to 6.30 in pairwise fashion, with an average of 1.32. Evolutionary relationship or closeness among strains of a fungal species can thus be said not to depend on location.
文摘This paper is a report of a 34-year-old man with chronic renal failure undergoing Continuous Ambulatory Peritoneal Dialysis which developed peritonitis due to Geotricum candidum. The diagnosis was established by culture of dialysis fluid. The purpose of this report is to provide data on a fungal peritonitis due to a non-common agent.
文摘从云南昆明地区的开菲尔粒中分离得到K2、Z7-1和Z8-4菌株,3株菌发酵产物可产生愉悦的花果香味。在基因组重测序基础上,分析菌株K2、Z7-1、Z8-4与白地霉(Geotrichum candidum)LMA-244基因组相似度,DNA杂交分析相似度分别为99.50%、99.40%、99.50%;平均核酸一致性分析,相似度分别为98.66%、98.65%、98.68%。结合形态特征和26S rDNA序列同源性分析,K2、Z7-1和Z8-43株菌均鉴定为白地霉。利用基因本体论、京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)、直系同源簇等数据库对白地霉K2进行基因功能注释,注释基因中40.74%与细胞组成相关,47.54%与生物过程相关,11.73%与分子功能相关。KEGG代谢途径分析发现萜类合成的相关基因,白地霉K2具有合成挥发性香味成分的遗传基础。采用气相色谱-质谱联用仪对白地霉K2菌株的发酵液挥发性成分进行检测分析,共检出50种挥发性成分,其中3-蒈烯、α-蒎烯和对伞花烃呈花香,L-α-松油醇呈松木香,4-萜烯醇、(-)-4-萜品醇呈丁香味,乙酸α-松油酯呈清甜香。推测白地霉是开菲尔酸奶香味物质的重要贡献菌株。本研究为白地霉K2用于发酵乳制品及其他发酵食品提供了理论基础。
文摘基于CiteSpace知识图谱与专利分析,探讨铁皮石斛的研究热点与发展趋势。通过系统检索中国知网(CNKI)数据库和Web of Science(WOS)核心合集数据库关于铁皮石斛的中、英文文献,运用CiteSpace 5.8.R3软件对关键词信息绘制知识图谱,进行铁皮石斛发展历程与研究热点的可视化分析,并结合专利计量学研究手段分析铁皮石斛相关专利的领域分布与发展趋势,以及铁皮石斛在食品应用开发方面的专利情况。结果表明,研究热点国内外各有侧重,国内专注于铁皮石斛多糖、保健功能、化学成分、产品开发及基因等研究,国外偏向于动物实验及安全性评价等临床实验研究,在活性成分鉴定、药理作用及作用机制等领域研究较多。专利计量分析发现铁皮石斛食品种类丰富且大多为明确保健功能的食品,共涉及9种保健功能,其中以增强免疫力为主,占比42.04%,铁皮石斛在食品领域可在不同保健功能开发,产品制备技术以及铁皮石斛花、叶上进一步进行研究。本研究为分析铁皮石斛的研究内容、判断研究热点及食品专利申请方向提供参考。
