It was believed that electroosmotic mobility μeo is inversely proportional to the square root of the ionic strength L But the linear relationship for regression analysis was expressed differently in different papers....It was believed that electroosmotic mobility μeo is inversely proportional to the square root of the ionic strength L But the linear relationship for regression analysis was expressed differently in different papers. The paper studied the linear expression of the mathematical relationship between μeo and c （background buffer concentration） by mathematical transform and real experimental data.μeo values of fused silica capillary were determined in four buffer systems. Their experimental conditions were controlled carefully for decreasing temperature difference AT and pH difference ApH in 50 μm ID capillary, in which no double layer overlap existed. The linear relationship between the reciprocal of electroosmotic mobility and the square root of concentration （or ionic strength） was derived by mathematical method. The regression analysis of experimental data was shown to well correspond to the relationship. The constants in regression equation could be well defined and the calculated results were acceptable.展开更多
Manipulation of electroosmotic flow in capillary electrophoresis is an importantstep for separation of inorganic anions.The type and concentratlon of electroosmotic flowmodifier (OFM) exert a tremendous influence on t...Manipulation of electroosmotic flow in capillary electrophoresis is an importantstep for separation of inorganic anions.The type and concentratlon of electroosmotic flowmodifier (OFM) exert a tremendous influence on the electroosmotic mobllity. ln the pres-ence of CTAB as buffer solutions’ pH values increase, the electroosmotic mobility becomeslower. At the same ionic strength, the buffer type affects the electroosmotic veloclty and themigration order for iodide and iodate.展开更多
A new method for the determination of the electroosmotic mobility was presented in this paper. The "sample zone" itself was used as a marker of the electroosmotic flow. The elution time of the sample zone fr...A new method for the determination of the electroosmotic mobility was presented in this paper. The "sample zone" itself was used as a marker of the electroosmotic flow. The elution time of the sample zone from the capillary was derived from the position of the electrophoretic current step. Based on it the mobility of electroosmotic flow can be calculated.The results was well in agreement with those of the neutral marker method. Furthermore,the present method was easy to operate and need no additive equipments.展开更多
文摘It was believed that electroosmotic mobility μeo is inversely proportional to the square root of the ionic strength L But the linear relationship for regression analysis was expressed differently in different papers. The paper studied the linear expression of the mathematical relationship between μeo and c （background buffer concentration） by mathematical transform and real experimental data.μeo values of fused silica capillary were determined in four buffer systems. Their experimental conditions were controlled carefully for decreasing temperature difference AT and pH difference ApH in 50 μm ID capillary, in which no double layer overlap existed. The linear relationship between the reciprocal of electroosmotic mobility and the square root of concentration （or ionic strength） was derived by mathematical method. The regression analysis of experimental data was shown to well correspond to the relationship. The constants in regression equation could be well defined and the calculated results were acceptable.
文摘Manipulation of electroosmotic flow in capillary electrophoresis is an importantstep for separation of inorganic anions.The type and concentratlon of electroosmotic flowmodifier (OFM) exert a tremendous influence on the electroosmotic mobllity. ln the pres-ence of CTAB as buffer solutions’ pH values increase, the electroosmotic mobility becomeslower. At the same ionic strength, the buffer type affects the electroosmotic veloclty and themigration order for iodide and iodate.
文摘A new method for the determination of the electroosmotic mobility was presented in this paper. The "sample zone" itself was used as a marker of the electroosmotic flow. The elution time of the sample zone from the capillary was derived from the position of the electrophoretic current step. Based on it the mobility of electroosmotic flow can be calculated.The results was well in agreement with those of the neutral marker method. Furthermore,the present method was easy to operate and need no additive equipments.
