Two new saponins named capilliposide C 1 and capilliposide D 2 were isolated from the whole plants of Lysimachia capillipes, their structures were deternuned by 1D and 2D NMR, ESIMS techniques, and chemical methods.Ca...Two new saponins named capilliposide C 1 and capilliposide D 2 were isolated from the whole plants of Lysimachia capillipes, their structures were deternuned by 1D and 2D NMR, ESIMS techniques, and chemical methods.Capilliposide C showed significant cytotoxic activity against human A2780 cells.展开更多
Objective:To investigate the potential effect of Lysimachia capillipes capilliposide(LCC)on the chemo sensitivity and the stemness of human ovarian cancer cells.Methods:Cell Counting Kit-8(CCK8)was used to measure the...Objective:To investigate the potential effect of Lysimachia capillipes capilliposide(LCC)on the chemo sensitivity and the stemness of human ovarian cancer cells.Methods:Cell Counting Kit-8(CCK8)was used to measure the IC50 values.The apoptosis of cells was measured through flow cytometry.Evaluation of the stemness and differentiation markers was performed by the immunoblotting and the immunostaining assays.RNA-seq was performed through the Illumina HiSeq PE150 platform and differentially expressed genes(DEGs)were screened out through the bioinformation analysis.Overexpression or knockdown of Fos gene was achieved by shRNA transfection.Results:Pre-exposure of A2780T cells with 10μg/mL LCC sensitized them to paclitaxel,of which the IC50 value reduced from 8.644μmol/L(95%CI:7.315–10.082μmol/L)to 2.5μmol/L(95%CI:2.233–2.7882μmol/L).Exposure with LCC enhanced the paclitaxel-induced apoptosis and inhibited the colony formation of A2780T cells.LCC exposure reduced the expression of cancer stemness markers,ALDH1,Myd88 and CD44,while promoting that of terminal differentiation markers,NFATc1,Cathepsin K and MMP9.RNAseq analysis revealed that the expressions of FOS and JUN were upregulated in LCC-treated A2780T cells.A2780T cells overexpressing Fos gene displayed increased paclitaxel-sensitivity and reduced cell stemness,and shared common phenotypes with LCC-treated A2780T cells.Conclusion:These findings suggested that LCC promoted terminal differentiations of ovarian cancer cells and sensitized them to paclitaxel through activating the Fos/Jun pathway.LCC might become a novel therapy that targets at cancer stem cells and enhances the chemotherapeutic effect of ovarian cancer treatments.展开更多
To investigate the saponins from whole plants of Lysimachia capilUpes Hemsl., two new saponins, named capilliposide E （1） and capilliposide F （2）, were isolated. The structures of the new saponins were elucidated ...To investigate the saponins from whole plants of Lysimachia capilUpes Hemsl., two new saponins, named capilliposide E （1） and capilliposide F （2）, were isolated. The structures of the new saponins were elucidated as 3β, 22α-dihydroxy-16α-acetat-28→ 13-lactone-oleanane-3-O- [β-D-glucopyranosyl- （1 →2）-α-L-arabinpyranoyl]-22-O-β-D-glucopyranoside （1） and 3β, 22α-dihydroxy- 16α-acetat-28→ 13-lactone-oleanane-3-O-{ [β-D-glucopyranosyl-（1→2）-β-D-glucopyranosyl-（1→4）]-α-L-arabinpyranoyl}-22-O-β- D-glucopyranoside （2）. The structures of these compounds were determined by 1D- and 2D-NMR, MS techniques, and chemical methods.展开更多
基金supported by the National Natural Science Foundation of China(Grant No.39870085)
文摘Two new saponins named capilliposide C 1 and capilliposide D 2 were isolated from the whole plants of Lysimachia capillipes, their structures were deternuned by 1D and 2D NMR, ESIMS techniques, and chemical methods.Capilliposide C showed significant cytotoxic activity against human A2780 cells.
基金supported by the Medical Science,Foundation of Zhejiang Province (No.LGF18H160087)
文摘Objective:To investigate the potential effect of Lysimachia capillipes capilliposide(LCC)on the chemo sensitivity and the stemness of human ovarian cancer cells.Methods:Cell Counting Kit-8(CCK8)was used to measure the IC50 values.The apoptosis of cells was measured through flow cytometry.Evaluation of the stemness and differentiation markers was performed by the immunoblotting and the immunostaining assays.RNA-seq was performed through the Illumina HiSeq PE150 platform and differentially expressed genes(DEGs)were screened out through the bioinformation analysis.Overexpression or knockdown of Fos gene was achieved by shRNA transfection.Results:Pre-exposure of A2780T cells with 10μg/mL LCC sensitized them to paclitaxel,of which the IC50 value reduced from 8.644μmol/L(95%CI:7.315–10.082μmol/L)to 2.5μmol/L(95%CI:2.233–2.7882μmol/L).Exposure with LCC enhanced the paclitaxel-induced apoptosis and inhibited the colony formation of A2780T cells.LCC exposure reduced the expression of cancer stemness markers,ALDH1,Myd88 and CD44,while promoting that of terminal differentiation markers,NFATc1,Cathepsin K and MMP9.RNAseq analysis revealed that the expressions of FOS and JUN were upregulated in LCC-treated A2780T cells.A2780T cells overexpressing Fos gene displayed increased paclitaxel-sensitivity and reduced cell stemness,and shared common phenotypes with LCC-treated A2780T cells.Conclusion:These findings suggested that LCC promoted terminal differentiations of ovarian cancer cells and sensitized them to paclitaxel through activating the Fos/Jun pathway.LCC might become a novel therapy that targets at cancer stem cells and enhances the chemotherapeutic effect of ovarian cancer treatments.
文摘To investigate the saponins from whole plants of Lysimachia capilUpes Hemsl., two new saponins, named capilliposide E （1） and capilliposide F （2）, were isolated. The structures of the new saponins were elucidated as 3β, 22α-dihydroxy-16α-acetat-28→ 13-lactone-oleanane-3-O- [β-D-glucopyranosyl- （1 →2）-α-L-arabinpyranoyl]-22-O-β-D-glucopyranoside （1） and 3β, 22α-dihydroxy- 16α-acetat-28→ 13-lactone-oleanane-3-O-{ [β-D-glucopyranosyl-（1→2）-β-D-glucopyranosyl-（1→4）]-α-L-arabinpyranoyl}-22-O-β- D-glucopyranoside （2）. The structures of these compounds were determined by 1D- and 2D-NMR, MS techniques, and chemical methods.
