Objective:To elucidate the effects of Huangqin Qingre Chubi capsule(HQC)on bone metabolism and serum TLR4 and NF-kB in rats with rheumatoid arthritis(RA),and to provide experimental bases for the treatment of RA with ...Objective:To elucidate the effects of Huangqin Qingre Chubi capsule(HQC)on bone metabolism and serum TLR4 and NF-kB in rats with rheumatoid arthritis(RA),and to provide experimental bases for the treatment of RA with HQC.Methods:A total of 40 SD rats were randomly divided into the normal group,model group,western medicine treatment group,and HQC group,with 10 rats in each group,and the RA model was induced by complete adjuvant in all groups except the normal group.After successful modeling,they were treated for 4 weeks to compare the degree of joint swelling;meanwhile,micro-CT was used to evaluate bone microstructural parameters,and changes in serum TLR4 and NF-kB expression levels were detected by ELISA.Results:There was no statistical significance in comparing the degree of joint swelling among the model group,western medicine treatment group and HQC group(P>0.05);Micro-CT results showed that bone microstructural parameters deteriorated in the osteoporosis model group compared with the normal group.Both western medicine and traditional Chinese medicine HQC could improve the bone microjunction of RA rats,and the HQC group was better than the western medicine treatment in improving the number of bone trabeculae(2.58±0.19)·mm^(-1);the serum results showed that RA was accompanied by the up-regulation of the expression of TLR4 and NF-kB.Both western drugs and HQC down-regulated the high expression of serum TLR4 and NF-kB in osteoporotic rats,and the down-regulation of serum TLR4(9.90±0.55)ng·ml^(-1)and NF-kB(350.29±3.14)ng·L^(-1)by HQC was more obvious.Conclusion:Chinese herbal medicine HQC can significantly improve the bone microstructure of RA rats,and its effect is better than that of western medicine in some aspects.The mechanism of action of HQC is related to the inhibition of the activation of the TLR4/NF-kB pathway,and this study provides an experimental basis for the further development and utilization of HQC.展开更多
目的：观察黄芩清热除痹胶囊口服联合芙蓉膏外敷治疗膝骨关节炎湿热痹阻型的临床疗效。方法60例膝骨关节炎患者，年龄40～70岁，中医辨证属湿热痹阻证者，随机分为治疗组30例与对照组30例，治疗组用黄芩清热除痹胶囊口服配合芙蓉膏外敷...目的：观察黄芩清热除痹胶囊口服联合芙蓉膏外敷治疗膝骨关节炎湿热痹阻型的临床疗效。方法60例膝骨关节炎患者，年龄40～70岁，中医辨证属湿热痹阻证者，随机分为治疗组30例与对照组30例，治疗组用黄芩清热除痹胶囊口服配合芙蓉膏外敷，疗程为2周，对照组给予氨基葡萄糖、美洛昔康口服治疗。结果治疗组总有效率96．7％，对照组总有效率76．7％，治疗组总有效率优于对照组，差异具有统计学意义（P ＜0．05）；两组治疗后均能显著降低患者中医证候总积分、关节疼痛 VAS 评分、WOMAC 指数、血沉（ESR），C －反应蛋白（CRP）水平（P ＜0．05或 P ＜0．01），两组治疗后比较，治疗组 VAS 评分、WOMAC 指数、ESR、CRP 下降的更明显（P ＜0．05或 P ＜0．01）；治疗组在上调超氧化物歧化酶（SOD）方面优于对照组（P＜0．05）；两组治疗后谷丙转氨酶（ALT）、谷草转氨酶（AST）、肌酐（Cre）、血常规均在正常范围。结论黄芩清热除痹胶囊口服联合芙蓉膏外敷能有效改善膝骨关节炎急性期症状，减轻患者疼痛 VAS 评分、WOMAC 指数、降低 ESR、CRP，起效迅速，且安全性良好，未见明显毒副作用。展开更多
文摘Objective:To elucidate the effects of Huangqin Qingre Chubi capsule(HQC)on bone metabolism and serum TLR4 and NF-kB in rats with rheumatoid arthritis(RA),and to provide experimental bases for the treatment of RA with HQC.Methods:A total of 40 SD rats were randomly divided into the normal group,model group,western medicine treatment group,and HQC group,with 10 rats in each group,and the RA model was induced by complete adjuvant in all groups except the normal group.After successful modeling,they were treated for 4 weeks to compare the degree of joint swelling;meanwhile,micro-CT was used to evaluate bone microstructural parameters,and changes in serum TLR4 and NF-kB expression levels were detected by ELISA.Results:There was no statistical significance in comparing the degree of joint swelling among the model group,western medicine treatment group and HQC group(P>0.05);Micro-CT results showed that bone microstructural parameters deteriorated in the osteoporosis model group compared with the normal group.Both western medicine and traditional Chinese medicine HQC could improve the bone microjunction of RA rats,and the HQC group was better than the western medicine treatment in improving the number of bone trabeculae(2.58±0.19)·mm^(-1);the serum results showed that RA was accompanied by the up-regulation of the expression of TLR4 and NF-kB.Both western drugs and HQC down-regulated the high expression of serum TLR4 and NF-kB in osteoporotic rats,and the down-regulation of serum TLR4(9.90±0.55)ng·ml^(-1)and NF-kB(350.29±3.14)ng·L^(-1)by HQC was more obvious.Conclusion:Chinese herbal medicine HQC can significantly improve the bone microstructure of RA rats,and its effect is better than that of western medicine in some aspects.The mechanism of action of HQC is related to the inhibition of the activation of the TLR4/NF-kB pathway,and this study provides an experimental basis for the further development and utilization of HQC.
文摘目的：观察黄芩清热除痹胶囊口服联合芙蓉膏外敷治疗膝骨关节炎湿热痹阻型的临床疗效。方法60例膝骨关节炎患者，年龄40～70岁，中医辨证属湿热痹阻证者，随机分为治疗组30例与对照组30例，治疗组用黄芩清热除痹胶囊口服配合芙蓉膏外敷，疗程为2周，对照组给予氨基葡萄糖、美洛昔康口服治疗。结果治疗组总有效率96．7％，对照组总有效率76．7％，治疗组总有效率优于对照组，差异具有统计学意义（P ＜0．05）；两组治疗后均能显著降低患者中医证候总积分、关节疼痛 VAS 评分、WOMAC 指数、血沉（ESR），C －反应蛋白（CRP）水平（P ＜0．05或 P ＜0．01），两组治疗后比较，治疗组 VAS 评分、WOMAC 指数、ESR、CRP 下降的更明显（P ＜0．05或 P ＜0．01）；治疗组在上调超氧化物歧化酶（SOD）方面优于对照组（P＜0．05）；两组治疗后谷丙转氨酶（ALT）、谷草转氨酶（AST）、肌酐（Cre）、血常规均在正常范围。结论黄芩清热除痹胶囊口服联合芙蓉膏外敷能有效改善膝骨关节炎急性期症状，减轻患者疼痛 VAS 评分、WOMAC 指数、降低 ESR、CRP，起效迅速，且安全性良好，未见明显毒副作用。
