During the screening of a traditional Chinese folk herb library against Hep G2 and Hep3 B cell lines, the Et OAc extract from the Tibetan medicine, Caragana tibetica（CT-Et OAc） exhibited potential anti-hepatocellula...During the screening of a traditional Chinese folk herb library against Hep G2 and Hep3 B cell lines, the Et OAc extract from the Tibetan medicine, Caragana tibetica（CT-Et OAc） exhibited potential anti-hepatocellular carcinoma（anti-HCC） activity. HPLC-based activity profiling was performed for targeted identification of anti-HCC activity from CT-Et OAc by MS-directed purification method. CT-Et OAc was separated by time-based fractionation for further anti-HCC bioassay by a semipreparative HPLC column（150 mm × 10 mm i.d., 5 μm） with a single injection of 5 mg. Bioassay-guided and ESIMS-directed large scale purification was performed with a single injection of 400 mg of CT-Et OAc by peak-based fractionation. A 1.4-mm heavy wall micro NMR tube with z-gradient was used to measure one and two dimensional NMR spectra for the minor or trace amounts of components of the extract. Two active compounds could be elucidated as naringenin chalcone（CT-1） and 3-hydroxy-8, 9-dimethoxypterocarpan（CT-2） relevant to anti-HCC effects for the Et OAc extract of C. tibetica rapidly and unambiguously by this protocol.展开更多
在内蒙古高原,藏锦鸡儿(Caragana tibetica)群落分布在草原向荒漠的过渡带上,在植被区划中常作为由草原进入荒漠的指示物种。藏锦鸡儿是一种旱生垫状矮灌木,由于其垫状生物学特性,在地表覆沙的生境中常形成较均匀的、非常醒目的灌丛沙...在内蒙古高原,藏锦鸡儿(Caragana tibetica)群落分布在草原向荒漠的过渡带上,在植被区划中常作为由草原进入荒漠的指示物种。藏锦鸡儿是一种旱生垫状矮灌木,由于其垫状生物学特性,在地表覆沙的生境中常形成较均匀的、非常醒目的灌丛沙堆。不同大小的灌丛沙堆可对应其不同的发育阶段,选取不同大小的藏锦鸡儿灌丛沙堆,划分成小、中、大3组,小、中两组灌丛沙堆对应其发育阶段,大沙堆组对应于稳定阶段,通过测定沙堆间、沙堆内(顶部、中部)及沙堆下的土壤有机质(Soil organic matters,SOM)、全磷(Total phosphorus,TP)和土壤质量含水量(Mass water content of soil,Wm),分析了藏锦鸡儿群落土壤资源的特点。结果显示,随着沙堆的发育,沙堆内和堆下0～20cm处的土壤有机质、全磷和土壤质量含水量的平均含量呈递增趋势,大沙堆、中沙堆及堆下0～20cm处的土壤有机质、全磷和土壤质量含水量的平均含量均大于沙堆间,小沙堆的土壤有机质、堆下0～20cm的土壤全磷和质量含水量的平均含量大于沙堆间。不同大小的灌丛沙堆,在垂直方向上,土壤有机质、全磷含量基本均呈先增加后减少的趋势,土壤质量含水量呈递减趋势;在水平方向,随着灌丛沙堆的发育,土壤有机质、全磷和土壤质量含水量在同一部位的同一土层基本呈递增趋势;不同大小的沙堆表层0～20cm处的土壤有机质由顶部、中部、沙堆间依次呈先增加后减小的趋势;大沙堆和中沙堆的全磷由沙堆顶部、中部、沙堆间依次呈递减趋势,而小沙堆土壤全磷呈先增加后减少的趋势。随着灌丛沙堆的发育,藏锦鸡儿灌丛截获的植物残体量呈增加趋势。研究显示,藏锦鸡儿在发育过程中形成"沃岛效应",这种效应不仅表现在沙堆内,也表现在沙堆下,同时也引起土壤有机质、全磷和土壤水分的空间异质性分布。展开更多
基金supported by grants from Natural Science Foundation of Qinghai Province(No.2012-z-904)the Major Research Project of Department of Education of Hubei Province,China(No.D20131103)+1 种基金National Natural Science Foundation of China(No.81102798)Wuhan Youth Chenguang Program of Science and Technology(No.2013070104010028)
文摘During the screening of a traditional Chinese folk herb library against Hep G2 and Hep3 B cell lines, the Et OAc extract from the Tibetan medicine, Caragana tibetica（CT-Et OAc） exhibited potential anti-hepatocellular carcinoma（anti-HCC） activity. HPLC-based activity profiling was performed for targeted identification of anti-HCC activity from CT-Et OAc by MS-directed purification method. CT-Et OAc was separated by time-based fractionation for further anti-HCC bioassay by a semipreparative HPLC column（150 mm × 10 mm i.d., 5 μm） with a single injection of 5 mg. Bioassay-guided and ESIMS-directed large scale purification was performed with a single injection of 400 mg of CT-Et OAc by peak-based fractionation. A 1.4-mm heavy wall micro NMR tube with z-gradient was used to measure one and two dimensional NMR spectra for the minor or trace amounts of components of the extract. Two active compounds could be elucidated as naringenin chalcone（CT-1） and 3-hydroxy-8, 9-dimethoxypterocarpan（CT-2） relevant to anti-HCC effects for the Et OAc extract of C. tibetica rapidly and unambiguously by this protocol.
文摘在内蒙古高原,藏锦鸡儿(Caragana tibetica)群落分布在草原向荒漠的过渡带上,在植被区划中常作为由草原进入荒漠的指示物种。藏锦鸡儿是一种旱生垫状矮灌木,由于其垫状生物学特性,在地表覆沙的生境中常形成较均匀的、非常醒目的灌丛沙堆。不同大小的灌丛沙堆可对应其不同的发育阶段,选取不同大小的藏锦鸡儿灌丛沙堆,划分成小、中、大3组,小、中两组灌丛沙堆对应其发育阶段,大沙堆组对应于稳定阶段,通过测定沙堆间、沙堆内(顶部、中部)及沙堆下的土壤有机质(Soil organic matters,SOM)、全磷(Total phosphorus,TP)和土壤质量含水量(Mass water content of soil,Wm),分析了藏锦鸡儿群落土壤资源的特点。结果显示,随着沙堆的发育,沙堆内和堆下0～20cm处的土壤有机质、全磷和土壤质量含水量的平均含量呈递增趋势,大沙堆、中沙堆及堆下0～20cm处的土壤有机质、全磷和土壤质量含水量的平均含量均大于沙堆间,小沙堆的土壤有机质、堆下0～20cm的土壤全磷和质量含水量的平均含量大于沙堆间。不同大小的灌丛沙堆,在垂直方向上,土壤有机质、全磷含量基本均呈先增加后减少的趋势,土壤质量含水量呈递减趋势;在水平方向,随着灌丛沙堆的发育,土壤有机质、全磷和土壤质量含水量在同一部位的同一土层基本呈递增趋势;不同大小的沙堆表层0～20cm处的土壤有机质由顶部、中部、沙堆间依次呈先增加后减小的趋势;大沙堆和中沙堆的全磷由沙堆顶部、中部、沙堆间依次呈递减趋势,而小沙堆土壤全磷呈先增加后减少的趋势。随着灌丛沙堆的发育,藏锦鸡儿灌丛截获的植物残体量呈增加趋势。研究显示,藏锦鸡儿在发育过程中形成"沃岛效应",这种效应不仅表现在沙堆内,也表现在沙堆下,同时也引起土壤有机质、全磷和土壤水分的空间异质性分布。
