The electrochemical behaviors of lomefloxacin at a single-wall carbon nanotube-modified glassy carbon electrode have been investigated by cyclic voltammetry.In a Britton-Robinson buffer (pH 4.5),lomefloxacin yields a ...The electrochemical behaviors of lomefloxacin at a single-wall carbon nanotube-modified glassy carbon electrode have been investigated by cyclic voltammetry.In a Britton-Robinson buffer (pH 4.5),lomefloxacin yields a sensitive and well-defined oxidation peak at ca.1.24 V (vs.SCE) on the modified electrode.Compared with the bare glassy carbon electrode,the oxidation peak current of lomefloxacin significantly increases and the oxidation peak potential positively shifts.Under the optimal conditions,the interaction of lomefloxacin with bovine serum albumin is also investigated.The results indicate that an electrochemically inactive supramolecular complex is formed and the formation of complex between lomefloxacin and bovine serum albumin is an intercalation mechanism.The proposed methods offer a reference for the studies on the biological effects and action mechanism of lomefloxacin with albumins in vivo.展开更多
In pH 5.5 phosphate buffer solution, N-n-undecyl-N＇-（sodium-p-amino-benzenesulfonate） thiourea （UPT） produced a pair of redox peaks on the bare glassy carbon electrode. At the multi-walled carbon nanotube （MWNT...In pH 5.5 phosphate buffer solution, N-n-undecyl-N＇-（sodium-p-amino-benzenesulfonate） thiourea （UPT） produced a pair of redox peaks on the bare glassy carbon electrode. At the multi-walled carbon nanotube （MWNT） modified electrode, the electrochemical behavior of UPT enhanced greatly. In the presence of bovine serum albumin （BSA）, the peak currents of UPT decreased linearly due to the formation of a super-molecular complex. This method was successfully applied to the determination of BSA in a bovine serum sample.展开更多
In this paper, Fourier transform infrared spectroscopy and inductively coupled plasma atomic emission spectrometry were used to study the filtrates produced by reactions of different initial mole ratios of bovine seru...In this paper, Fourier transform infrared spectroscopy and inductively coupled plasma atomic emission spectrometry were used to study the filtrates produced by reactions of different initial mole ratios of bovine serum albumin (BSA) and calcium carbonate (CaCO3) in saline solution. The results demonstrated that there were strong interactions between BSA and calcium carbonate by hydrogen bonds or coordination or electrostatic attraction, which made the solubility of calcium carbonate in aqueous solution increased and the microstructure of BSA and CaCO3 changed. The interaction helped to form biomineral containing protein and CaCO3 in organism.展开更多
以电化学法和光度法对铬黑T（Eriochrome black T,EBT）与牛血清白蛋白（Bovine serum albumin,BSA）的相互作用进行了研究。研究表明：在pH5.29的（Britton-Robinson,B-R）缓冲液中,EBT有两个氧化峰和两个还原峰,加入BSA后EBT峰电位不...以电化学法和光度法对铬黑T（Eriochrome black T,EBT）与牛血清白蛋白（Bovine serum albumin,BSA）的相互作用进行了研究。研究表明：在pH5.29的（Britton-Robinson,B-R）缓冲液中,EBT有两个氧化峰和两个还原峰,加入BSA后EBT峰电位不变,峰电流下降,据此建立了一种测定BSA的电分析方法。在优化条件下,峰电流下降值与所加的BSA量在2.0×10-6~1.0×10-5mol/L、2.0×10-6~6.0×10-7mol/L和6.0×10-7~5.0×10-8mol/L范围内呈线性响应,检测限为1.0×10-9mol/L。通过测定BSA引入前后的一些电化学参数,推测EBT与BSA相互作用生成了一种非电活性的超分子化合物,探讨了BSA与EBT的结合反应机理。展开更多
基金National Natural Science Foundation of China (No. 20775047)the Natural Science Foundation of Henan Province (No. 0511022300)
文摘The electrochemical behaviors of lomefloxacin at a single-wall carbon nanotube-modified glassy carbon electrode have been investigated by cyclic voltammetry.In a Britton-Robinson buffer (pH 4.5),lomefloxacin yields a sensitive and well-defined oxidation peak at ca.1.24 V (vs.SCE) on the modified electrode.Compared with the bare glassy carbon electrode,the oxidation peak current of lomefloxacin significantly increases and the oxidation peak potential positively shifts.Under the optimal conditions,the interaction of lomefloxacin with bovine serum albumin is also investigated.The results indicate that an electrochemically inactive supramolecular complex is formed and the formation of complex between lomefloxacin and bovine serum albumin is an intercalation mechanism.The proposed methods offer a reference for the studies on the biological effects and action mechanism of lomefloxacin with albumins in vivo.
基金the National Natural Science Foundation of China(No.20575077).
文摘In pH 5.5 phosphate buffer solution, N-n-undecyl-N＇-（sodium-p-amino-benzenesulfonate） thiourea （UPT） produced a pair of redox peaks on the bare glassy carbon electrode. At the multi-walled carbon nanotube （MWNT） modified electrode, the electrochemical behavior of UPT enhanced greatly. In the presence of bovine serum albumin （BSA）, the peak currents of UPT decreased linearly due to the formation of a super-molecular complex. This method was successfully applied to the determination of BSA in a bovine serum sample.
文摘In this paper, Fourier transform infrared spectroscopy and inductively coupled plasma atomic emission spectrometry were used to study the filtrates produced by reactions of different initial mole ratios of bovine serum albumin (BSA) and calcium carbonate (CaCO3) in saline solution. The results demonstrated that there were strong interactions between BSA and calcium carbonate by hydrogen bonds or coordination or electrostatic attraction, which made the solubility of calcium carbonate in aqueous solution increased and the microstructure of BSA and CaCO3 changed. The interaction helped to form biomineral containing protein and CaCO3 in organism.
文摘以电化学法和光度法对铬黑T（Eriochrome black T,EBT）与牛血清白蛋白（Bovine serum albumin,BSA）的相互作用进行了研究。研究表明：在pH5.29的（Britton-Robinson,B-R）缓冲液中,EBT有两个氧化峰和两个还原峰,加入BSA后EBT峰电位不变,峰电流下降,据此建立了一种测定BSA的电分析方法。在优化条件下,峰电流下降值与所加的BSA量在2.0×10-6~1.0×10-5mol/L、2.0×10-6~6.0×10-7mol/L和6.0×10-7~5.0×10-8mol/L范围内呈线性响应,检测限为1.0×10-9mol/L。通过测定BSA引入前后的一些电化学参数,推测EBT与BSA相互作用生成了一种非电活性的超分子化合物,探讨了BSA与EBT的结合反应机理。
