The rice false smut disease, caused by Ustilaginoidea virens, has emerged as a significantglobal threat to rice production. The mechanism of carbon catabolite repression plays a crucial role in theefficient utilizatio...The rice false smut disease, caused by Ustilaginoidea virens, has emerged as a significantglobal threat to rice production. The mechanism of carbon catabolite repression plays a crucial role in theefficient utilization of carbon nutrients and enzyme regulation in the presence of complex nutritionalconditions. Although significant progress has been made in understanding carbon catabolite repression infungi such as Aspergillus nidulans and Magnaporthe oryzae, its role in U. virens remains unclear. Toaddress this knowledge gap, we identified UvCreA, a pivotal component of carbon catabolite repression,in U. virens. Our investigation revealed that UvCreA localized to the nucleus. Deletion of UvCreA resultedin decreased growth and pathogenicity in U. virens. Through RNA-seq analysis, it was found that theknockout of UvCreA led to the up-regulation of 514 genes and down-regulation of 640 genes. Moreover,UvCreA was found to be involved in the transcriptional regulation of pathogenic genes and genesassociated with carbon metabolism in U. virens. In summary, our findings indicated that UvCreA isimportant in fungal development, virulence, and the utilization of carbon sources through transcriptionalregulation, thus making it a critical element of carbon catabolite repression.展开更多
Carbon catabolite repression(CCR)plays a key role in many physiological and adaptive responses in a broad range of microorganisms that are commonly associated with eukaryotic hosts.When a mixture of different carbon s...Carbon catabolite repression(CCR)plays a key role in many physiological and adaptive responses in a broad range of microorganisms that are commonly associated with eukaryotic hosts.When a mixture of different carbon sources is available,CCR,a global regulatory mechanism,inhibits the expression and activity of cellular processes associated with utilization of secondary carbon sources in the presence of the preferred carbon source.CCR is known to be executed by completely different mechanisms in different bacteria,yeast,and fungi.In addition to regulating catabolic genes,CCR also appears to play a key role in the expression of genes involved in plant–microbe interactions.Here,we present a detailed overview of CCR mechanisms in various bacteria.We highlight the role of CCR in beneficial as well as deleterious plant–microbe interactions based on the available literature.In addition,we explore the global distribution of known regulatory mechanisms within bacterial genomes retrieved from public repositories and within metatranscriptomes obtained from different plant rhizospheres.By integrating the available literature and performing targeted meta-analyses,we argue that CCR-regulated substrate use preferences of microorganisms should be considered an important trait involved in prevailing plant–microbe interactions.展开更多
Carbon catabolite repression(CCR),which is mainly mediated by Cre1 and triggered by glucose,leads to a decrease in cellulase production in Trichoderma reesei.Many studies have focused on modifying Cre1 for alleviating...Carbon catabolite repression(CCR),which is mainly mediated by Cre1 and triggered by glucose,leads to a decrease in cellulase production in Trichoderma reesei.Many studies have focused on modifying Cre1 for alleviating CCR.Based on the homologous alignment of CreA from wild-type Penicillium oxalicum 114–2(Po-0)and cellulase hyperproducer JUA10-1(Po-1),we constructed a C-terminus substitution strain—Po-2—with decreased transcriptional levels of cellulase and enhanced CCR.Results revealed that the C-terminal domain of CreAPo−1 plays an important role in alleviating CCR.Furthermore,we replaced the C-terminus of Cre1 with that of CreAPo−1 in T.reesei(Tr-0)and generated Tr-1.As a control,the C-terminus of Cre1 was truncated and Tr-2 was generated.The transcriptional profiles of these transformants revealed that the C-terminal chimera greatly improves cellulase transcription in the presence of glucose and thus upregulates cellulase in the presence of glucose and weakens CCR,consistent with truncating the C-terminus of Cre1 in Tr-0.Therefore,we propose constructing a C-terminal chimera as a new strategy to improve cellulase production and alleviate CCR in the presence of glucose.展开更多
代谢调控蛋白A(catabolite control protein A,CcpA)是革兰阳性菌重要的多效调节因子,在磷酸烯醇丙酮酸依赖的磷酸转移酶系统辅助下,能与靶基因上的特异性DNA序列——代谢反应元件结合,促进或阻遏靶基因表达,从而调控细菌的生理过程和...代谢调控蛋白A(catabolite control protein A,CcpA)是革兰阳性菌重要的多效调节因子,在磷酸烯醇丙酮酸依赖的磷酸转移酶系统辅助下,能与靶基因上的特异性DNA序列——代谢反应元件结合,促进或阻遏靶基因表达,从而调控细菌的生理过程和毒力。CcpA通过介导微生物碳分解代谢物阻遏效应,控制细菌摄取与利用外界环境中营养物质,保障其高效利用能源。CcpA通过直接或间接调控细菌的代谢过程和毒力基因表达,将细菌的代谢与毒力偶联。该文介绍了CcpA的作用机制及其对细菌多种代谢途径和致病性调控的研究进展。展开更多
The nitrogen source requirements for riboflavin production by ccpA mutant Bacillus subtilis 24A1/pMX45 were optimized using linear regression. The optimal medium components considered included 8% glucose as carbon sou...The nitrogen source requirements for riboflavin production by ccpA mutant Bacillus subtilis 24A1/pMX45 were optimized using linear regression. The optimal medium components considered included 8% glucose as carbon source, 2% yeast powder, 0.05% MgSO4 ·7H2O, and four types of nitrogen sources ： 0.1% yeast extract, 2% soybean powder, 1% corn plasm, and 0.2% （ NH4 ） 2 HPO4 in shake flask tests. Predictive ellipsoid was applied to determining the response values under the optimal levels for riboflavin production and glucose consumption. The optimal concentrations of the four types of nitrogen sources can remedy ammonium assimilative defection of ccpA mutant. Under the optimal conditions, the riboflavin yield increases to more than 5.0 g/L and 8%, glucose can be consumed completely after 60 h.展开更多
代谢调控蛋白A(catabolite control protein A,CcpA)是革兰氏阳性菌重要的全局性转录调节因子,通过与靶基因代谢反应元件(cre基序)结合,调控基因的转录与表达,参与碳分解代谢物阻遏效应、物质代谢、毒力及抗菌药物耐受等众多生命活动的...代谢调控蛋白A(catabolite control protein A,CcpA)是革兰氏阳性菌重要的全局性转录调节因子,通过与靶基因代谢反应元件(cre基序)结合,调控基因的转录与表达,参与碳分解代谢物阻遏效应、物质代谢、毒力及抗菌药物耐受等众多生命活动的调节。本文就CcpA蛋白的分子结构、作用机制、表达调控、生物学功能等方面的研究进展进行综述,以增加对CcpA蛋白及细菌复杂转录调控网络的认识,为革兰氏阳性菌感染的防控提供潜在靶标。展开更多
碳分解代谢物阻遏(carbon catabolite repression,CCR)是指微生物在混合碳源发酵时优先利用速效碳源(通常为葡萄糖),且该碳源的代谢产物会抑制其他非速效碳源代谢相关的基因表达和蛋白活性,从而影响非速效碳源利用的现象。在低GC含量革...碳分解代谢物阻遏(carbon catabolite repression,CCR)是指微生物在混合碳源发酵时优先利用速效碳源(通常为葡萄糖),且该碳源的代谢产物会抑制其他非速效碳源代谢相关的基因表达和蛋白活性,从而影响非速效碳源利用的现象。在低GC含量革兰氏阳性菌中,CCR效应的关键调控因子为分解代谢物控制蛋白CcpA(catabolite control protein A)。该调控蛋白具有多效性功能,除参与CCR外,还与中心碳、氮代谢的调控、生物被膜的形成和毒性基因的表达等多种生理过程相关。综述了近年来有关CcpA蛋白的功能、作用机制及分子结构的研究进展。展开更多
基金the Key Projects of Zhejiang Provincial Natural Science Foundation,China(Grant No.LZ23C130002)the National Natural Science Foundation of China(Grant No.32100161)+3 种基金the Zhejiang Science and Technology Major Program on Rice New Variety Breeding,China(Grant No.2021C02063)the Key R&D Project of China National Rice Research Institute(Grant No.CNRRI-2020-04)the Chinese Academy of Agricultural Sciences under the Agricultural Sciences and Technologies Innovation Program,the Youth innovation Program of Chinese Academy of Agricultural Sciences(Grant No.Y2023QC22)the Joint Open Competitive Project of the Yazhou Bay Seed Laboratory and China National Seed Company Limited(Grant Nos.B23YQ1514 and B23CQ15EP).
文摘The rice false smut disease, caused by Ustilaginoidea virens, has emerged as a significantglobal threat to rice production. The mechanism of carbon catabolite repression plays a crucial role in theefficient utilization of carbon nutrients and enzyme regulation in the presence of complex nutritionalconditions. Although significant progress has been made in understanding carbon catabolite repression infungi such as Aspergillus nidulans and Magnaporthe oryzae, its role in U. virens remains unclear. Toaddress this knowledge gap, we identified UvCreA, a pivotal component of carbon catabolite repression,in U. virens. Our investigation revealed that UvCreA localized to the nucleus. Deletion of UvCreA resultedin decreased growth and pathogenicity in U. virens. Through RNA-seq analysis, it was found that theknockout of UvCreA led to the up-regulation of 514 genes and down-regulation of 640 genes. Moreover,UvCreA was found to be involved in the transcriptional regulation of pathogenic genes and genesassociated with carbon metabolism in U. virens. In summary, our findings indicated that UvCreA isimportant in fungal development, virulence, and the utilization of carbon sources through transcriptionalregulation, thus making it a critical element of carbon catabolite repression.
基金This work was supported by the French National Research Agency(ANR-18-CE32-0005,DIORE).
文摘Carbon catabolite repression(CCR)plays a key role in many physiological and adaptive responses in a broad range of microorganisms that are commonly associated with eukaryotic hosts.When a mixture of different carbon sources is available,CCR,a global regulatory mechanism,inhibits the expression and activity of cellular processes associated with utilization of secondary carbon sources in the presence of the preferred carbon source.CCR is known to be executed by completely different mechanisms in different bacteria,yeast,and fungi.In addition to regulating catabolic genes,CCR also appears to play a key role in the expression of genes involved in plant–microbe interactions.Here,we present a detailed overview of CCR mechanisms in various bacteria.We highlight the role of CCR in beneficial as well as deleterious plant–microbe interactions based on the available literature.In addition,we explore the global distribution of known regulatory mechanisms within bacterial genomes retrieved from public repositories and within metatranscriptomes obtained from different plant rhizospheres.By integrating the available literature and performing targeted meta-analyses,we argue that CCR-regulated substrate use preferences of microorganisms should be considered an important trait involved in prevailing plant–microbe interactions.
基金This work was supported by National Key R&D Program of China(No.2018YFA0901700)National Natural Science Foundation of China(No.31870785 and 31570040)+1 种基金the 111 Project(No.B16030)the State Key Laboratory of Microbial Technology Open Projects Fund.
文摘Carbon catabolite repression(CCR),which is mainly mediated by Cre1 and triggered by glucose,leads to a decrease in cellulase production in Trichoderma reesei.Many studies have focused on modifying Cre1 for alleviating CCR.Based on the homologous alignment of CreA from wild-type Penicillium oxalicum 114–2(Po-0)and cellulase hyperproducer JUA10-1(Po-1),we constructed a C-terminus substitution strain—Po-2—with decreased transcriptional levels of cellulase and enhanced CCR.Results revealed that the C-terminal domain of CreAPo−1 plays an important role in alleviating CCR.Furthermore,we replaced the C-terminus of Cre1 with that of CreAPo−1 in T.reesei(Tr-0)and generated Tr-1.As a control,the C-terminus of Cre1 was truncated and Tr-2 was generated.The transcriptional profiles of these transformants revealed that the C-terminal chimera greatly improves cellulase transcription in the presence of glucose and thus upregulates cellulase in the presence of glucose and weakens CCR,consistent with truncating the C-terminus of Cre1 in Tr-0.Therefore,we propose constructing a C-terminal chimera as a new strategy to improve cellulase production and alleviate CCR in the presence of glucose.
文摘代谢调控蛋白A(catabolite control protein A,CcpA)是革兰阳性菌重要的多效调节因子,在磷酸烯醇丙酮酸依赖的磷酸转移酶系统辅助下,能与靶基因上的特异性DNA序列——代谢反应元件结合,促进或阻遏靶基因表达,从而调控细菌的生理过程和毒力。CcpA通过介导微生物碳分解代谢物阻遏效应,控制细菌摄取与利用外界环境中营养物质,保障其高效利用能源。CcpA通过直接或间接调控细菌的代谢过程和毒力基因表达,将细菌的代谢与毒力偶联。该文介绍了CcpA的作用机制及其对细菌多种代谢途径和致病性调控的研究进展。
文摘The nitrogen source requirements for riboflavin production by ccpA mutant Bacillus subtilis 24A1/pMX45 were optimized using linear regression. The optimal medium components considered included 8% glucose as carbon source, 2% yeast powder, 0.05% MgSO4 ·7H2O, and four types of nitrogen sources ： 0.1% yeast extract, 2% soybean powder, 1% corn plasm, and 0.2% （ NH4 ） 2 HPO4 in shake flask tests. Predictive ellipsoid was applied to determining the response values under the optimal levels for riboflavin production and glucose consumption. The optimal concentrations of the four types of nitrogen sources can remedy ammonium assimilative defection of ccpA mutant. Under the optimal conditions, the riboflavin yield increases to more than 5.0 g/L and 8%, glucose can be consumed completely after 60 h.
文摘代谢调控蛋白A(catabolite control protein A,CcpA)是革兰氏阳性菌重要的全局性转录调节因子,通过与靶基因代谢反应元件(cre基序)结合,调控基因的转录与表达,参与碳分解代谢物阻遏效应、物质代谢、毒力及抗菌药物耐受等众多生命活动的调节。本文就CcpA蛋白的分子结构、作用机制、表达调控、生物学功能等方面的研究进展进行综述,以增加对CcpA蛋白及细菌复杂转录调控网络的认识,为革兰氏阳性菌感染的防控提供潜在靶标。
文摘碳分解代谢物阻遏(carbon catabolite repression,CCR)是指微生物在混合碳源发酵时优先利用速效碳源(通常为葡萄糖),且该碳源的代谢产物会抑制其他非速效碳源代谢相关的基因表达和蛋白活性,从而影响非速效碳源利用的现象。在低GC含量革兰氏阳性菌中,CCR效应的关键调控因子为分解代谢物控制蛋白CcpA(catabolite control protein A)。该调控蛋白具有多效性功能,除参与CCR外,还与中心碳、氮代谢的调控、生物被膜的形成和毒性基因的表达等多种生理过程相关。综述了近年来有关CcpA蛋白的功能、作用机制及分子结构的研究进展。
