血清CEA及CA153连续且显著降低对晚期乳腺癌无影像学进展的判定价值

目的:探讨晚期乳腺癌患者化疗后血清CEA及CA153连续且显著降低对无影像学进展的判定价值。方法:回顾性分析2011年1月至2015年12月93例沈阳军区总医院住院化疗的晚期乳腺癌患者的病历资料,实施疗效评价188次,有完整CEA、CA153数据的病例分别为105、139例次,分析血清CEA及CA153连续且显著降低对于疾病无影像学进展的判定效度。结果:CEA连续降低且降低总幅度达10%时,敏感度为54.1%,特异度为95%;CA153连续降低且降低总幅度达40%时,敏感度为19.1%,特异度为96.6%。结论:血清CEA及CA153连续降低分别达10%与40%以上时可作为晚期乳腺癌患者的疗效判定指标。

Construction of CEA siRNA expression vector and its inhibitory effects on the expression of CEA in EC9706 cells

Objective: To construct the small interfering RNA (siRNA) expression vector of carcino-embryonic antigen (CEA) and inhibit the expression of CEA in EC9706 cells by RNA interference. Methods: Two pairs of oligonucleotide sequences were designed and synthesized according to the encoding sequence of mRNA of CEA. The annealed oligonucleotide frag-ments were cloned into pRNAT-U6.2 expression vector and identified by sequencing. The recombinant plasmid pRNAT-U6.2-CEA was transfected into EC9706 cells. The expression of CEA in the stable transfected cells was assayed by real time PCR and Western blot. Results: DNA sequencing showed that the oligonucleotide fragments were correctly inserted into pRNAT-U6.2 vector, and CEA expression in the transfected cells was down-regulated significantly by pRNAT-U6.2-CEA at both the mRNA and protein levels. Conclusion: The siRNA expression vector of CEA is successfully constructed and inhibits CEA expression in EC9706 cells. This facilitates further studies of the function of CEA at the molecular level.