The effect of ginsenoside Rb 1 on cardiomyocyte apotosis after ischemia (30 min ) and reperfusion (6 h) in rats was observed. The ischemia/ reperfusion heart mo del was established by ligating left anterior descendin...The effect of ginsenoside Rb 1 on cardiomyocyte apotosis after ischemia (30 min ) and reperfusion (6 h) in rats was observed. The ischemia/ reperfusion heart mo del was established by ligating left anterior descending branch of coronary arte ry in Wistar rats. The apoptotic cardiomyocytes were examined under transmission electron microscopy and counted by in situ nick end labeling (TUNEL) method and light microscopy. Results showed that (1) The apoptotic cardiomyocytes were found in ischemic regions in the ischemia/reperfusion group, but not in the sh am oper ating group under transmission electron microscopy; (2) The number of apoptotic cells were 134.45±45.61/field in the ischemia/reperfusion group, 0/field in the sham operating group and 51.65±13.71/field in the ginsenoside Rb 1 treated group. The differences were significant among the three groups ( P <0.01). It was concluded that myocardial ischemia reperfusion could induce cardiomyocyte a poptosis, and ginsenoside Rb 1 could significantly inhibit cardiomyocyte apopto sis induced by ischemia reperfusion in rats, indicating that ginsenoside Rb 1 could inhibit cardiomyocyte apoptosis induced by ischemia reperfusion, thus alleviating ischemia reperfusion injury.展开更多
Objective To explore the mechanism of effects o f cardiac sympathetic anesthesia on left ventricular ejection fraction(LVEF)and left cardiac cavity size of patie nts with dilated cardiomyopathy.Method121consecutive pa...Objective To explore the mechanism of effects o f cardiac sympathetic anesthesia on left ventricular ejection fraction(LVEF)and left cardiac cavity size of patie nts with dilated cardiomyopathy.Method121consecutive patients with dilat ed cardiomyopathy were divided into cardiac sympathetic nerve blockade group(TEAgroup)and control group(c group).In TEAgroup,5%lidocaine was injected into thoracic epidural cavity for about 4to 8weeks in addition with routine therapy.In c group,only routine therapy was use d.We observe the changes of LVEF and left cardiac cavity size before a nd after treatment in both groups.Result I n TEAgroup,after anesthesia,LVEF w as increased from(31.3±12.8)to(47.3±21.3),P <0.001;left ventricular end -dia stolic diameter was reduced from(69.1±7.1)to(65.1±8.0),P <0.001;left atrial diameter was decreased from(44.0±6.2)to(39.4±7.2),P <0.001.Conclusion Cardiac sympathetic anesthesia can effectively improve the ejectio n performance of dilated cardiomyopathy and make the dilated cardiac cavity turn to normal level.展开更多
OBJECTIVE: To investigate the effect of Shenfuqiangxin capsule and the underlying mechanism on cardio-renal syndrome(CRS) in rats induced by infrarenal aortic-clamping after renal ischaemia.METHODS: Male Wistar rats u...OBJECTIVE: To investigate the effect of Shenfuqiangxin capsule and the underlying mechanism on cardio-renal syndrome(CRS) in rats induced by infrarenal aortic-clamping after renal ischaemia.METHODS: Male Wistar rats underwent infrarenal aortic-clamping after renal ischaemia or sham operation. The surviving CRS rats were divided randomly into three groups: CRS group(CRS + 10 m L·kg-1·d-1pure water by gavage), SFQX group(CRS + 13.2 g crude drug·kg- 1·d- 1Shenfuqiangxin by gavage),and handleregionpeptide(HRP)group(CRS+10mg/kg HRP by vein). Sham operation rats were given10 m L·kg-1·d-1pure water. Treatments were given8 weeks after surgery, which lasted for 4 weeks. Therats were detected for heart structure and function by transthoracic echocardiography. PPR m RNA was detected by Reverse Transcription Polymerase Chain Reaction(RT-PCR). To determine whether the mitogen-activated protein kinase(MAPK) signal pathway is included in the heart and kidney protective function of Shenfuqiangxin capsule, MAPK related proteins such as posophorylated C-Jun amino terminal kinase(p-JNK), posophorylated extracellular signal-regulated kinase ?(p-ERK1/2), posophorylated p38(p-p38) were examined by Western Blot.Apoptosis in heart and kidney tissues were detected by d UTP Nick End Labeling staining.RESULTS: Shenfuqiangxin capsule alleviated myocardial apoptosis and inhibited PRR m RNA expression and p-JNK, p-ERK1/2, p-p38 proteins expression in CRS rats.CONCLUSION: All the results suggest that Shenfuqiangxin capsule improves the injured heart and kidney function maybe through inhibition of MAPK response pathway.展开更多
基金a grantfrom the NaturalScience Foundation of Hubei Province (Serial No.2 0 0 0 J0 5 0 )
文摘The effect of ginsenoside Rb 1 on cardiomyocyte apotosis after ischemia (30 min ) and reperfusion (6 h) in rats was observed. The ischemia/ reperfusion heart mo del was established by ligating left anterior descending branch of coronary arte ry in Wistar rats. The apoptotic cardiomyocytes were examined under transmission electron microscopy and counted by in situ nick end labeling (TUNEL) method and light microscopy. Results showed that (1) The apoptotic cardiomyocytes were found in ischemic regions in the ischemia/reperfusion group, but not in the sh am oper ating group under transmission electron microscopy; (2) The number of apoptotic cells were 134.45±45.61/field in the ischemia/reperfusion group, 0/field in the sham operating group and 51.65±13.71/field in the ginsenoside Rb 1 treated group. The differences were significant among the three groups ( P <0.01). It was concluded that myocardial ischemia reperfusion could induce cardiomyocyte a poptosis, and ginsenoside Rb 1 could significantly inhibit cardiomyocyte apopto sis induced by ischemia reperfusion in rats, indicating that ginsenoside Rb 1 could inhibit cardiomyocyte apoptosis induced by ischemia reperfusion, thus alleviating ischemia reperfusion injury.
文摘Objective To explore the mechanism of effects o f cardiac sympathetic anesthesia on left ventricular ejection fraction(LVEF)and left cardiac cavity size of patie nts with dilated cardiomyopathy.Method121consecutive patients with dilat ed cardiomyopathy were divided into cardiac sympathetic nerve blockade group(TEAgroup)and control group(c group).In TEAgroup,5%lidocaine was injected into thoracic epidural cavity for about 4to 8weeks in addition with routine therapy.In c group,only routine therapy was use d.We observe the changes of LVEF and left cardiac cavity size before a nd after treatment in both groups.Result I n TEAgroup,after anesthesia,LVEF w as increased from(31.3±12.8)to(47.3±21.3),P <0.001;left ventricular end -dia stolic diameter was reduced from(69.1±7.1)to(65.1±8.0),P <0.001;left atrial diameter was decreased from(44.0±6.2)to(39.4±7.2),P <0.001.Conclusion Cardiac sympathetic anesthesia can effectively improve the ejectio n performance of dilated cardiomyopathy and make the dilated cardiac cavity turn to normal level.
基金Natural Science Foundation-funded Project:Study on Mechanism of Invigorate Qi Warm Yang Activate Blood And Promote Dieresis Treatment Adjusted By MAPK Mediated by(P)RR(No.30973795)Sponsored by the the National Natural Science Foundation of China(No.81202801)
文摘OBJECTIVE: To investigate the effect of Shenfuqiangxin capsule and the underlying mechanism on cardio-renal syndrome(CRS) in rats induced by infrarenal aortic-clamping after renal ischaemia.METHODS: Male Wistar rats underwent infrarenal aortic-clamping after renal ischaemia or sham operation. The surviving CRS rats were divided randomly into three groups: CRS group(CRS + 10 m L·kg-1·d-1pure water by gavage), SFQX group(CRS + 13.2 g crude drug·kg- 1·d- 1Shenfuqiangxin by gavage),and handleregionpeptide(HRP)group(CRS+10mg/kg HRP by vein). Sham operation rats were given10 m L·kg-1·d-1pure water. Treatments were given8 weeks after surgery, which lasted for 4 weeks. Therats were detected for heart structure and function by transthoracic echocardiography. PPR m RNA was detected by Reverse Transcription Polymerase Chain Reaction(RT-PCR). To determine whether the mitogen-activated protein kinase(MAPK) signal pathway is included in the heart and kidney protective function of Shenfuqiangxin capsule, MAPK related proteins such as posophorylated C-Jun amino terminal kinase(p-JNK), posophorylated extracellular signal-regulated kinase ?(p-ERK1/2), posophorylated p38(p-p38) were examined by Western Blot.Apoptosis in heart and kidney tissues were detected by d UTP Nick End Labeling staining.RESULTS: Shenfuqiangxin capsule alleviated myocardial apoptosis and inhibited PRR m RNA expression and p-JNK, p-ERK1/2, p-p38 proteins expression in CRS rats.CONCLUSION: All the results suggest that Shenfuqiangxin capsule improves the injured heart and kidney function maybe through inhibition of MAPK response pathway.
