High-sensitivity cardiac troponin(hs-cTn) assays are increasingly being used in many countries worldwide,however,a generally accepted definition of high-sen-sitivity is still pending.These assays enable cTn mea-sureme...High-sensitivity cardiac troponin(hs-cTn) assays are increasingly being used in many countries worldwide,however,a generally accepted definition of high-sen-sitivity is still pending.These assays enable cTn mea-surement with a high degree of analytical sensitivity with a low analytical imprecision at the low measuring range of cTn assays(coefficient of variation of < 10% at the 99th percentile upper reference limit).One of the most important advantages of these new assays is that they allow novel,more rapid approaches to rule in or rule out acute coronary syndromes(ACSs) than with previous cTn assay generations which are still more commonly used in practice worldwide.hs-cTn is also more sensitive for the detection of myocardial damage unrelated to acute myocardial ischemia.Therefore,the increase in early diagnostic sensitivity of hs-cTn assays for ACS comes at the cost of a reduced ACS specificity,because more patients with other causes of acute or chronic myocardial injury without overt myocardial isch-emia are detected than with previous cTn assays.As hs-cTn assays are increasingly being adopted in clinical practice and more hs-cTn assays are being developed,this review attempts to synthesize the available clinical data to make recommendations for their everyday clini-cal routine use.展开更多
BACKGROUND:Early reperfusion can effectively treat acute myocardial infarction(AMI) and reduce the mortality signif icantly. This study aimed to compare the role of plasma microRNA-1(miR-1) and cardiac troponin T(cTnT...BACKGROUND:Early reperfusion can effectively treat acute myocardial infarction(AMI) and reduce the mortality signif icantly. This study aimed to compare the role of plasma microRNA-1(miR-1) and cardiac troponin T(cTnT) in early diagnosis of AMI patients.METHODS:From May 2011 to May 2012,plasma samples were collected from 56 AMI patients and 28 non-AMI controls. The expression of plasma miR-1 was measured by quantitative reverse transcription-polymerase chain reaction(qRT-PCR),and the level of plasma cTnT was measured using electrochemiluminescence-based methods on an Elecsys 2010 Immunoassay Analyzer. SPSS 16.0 was used for the statistical analysis of the results. Data were expressed as mean±standard deviation unless otherwise described. The differences about clinical characteristics between the AMI patients and controls were tested using Student's t test or Fisher's exact test. The Mann-Whitney U test was conducted to compare the expression of microRNAs between the AMI patients and controls. MicroRNAs expression between different intervals of the AMI patients was compared using Wilcoxon's signed-rank test. The receiver operating characteristic(ROC) curve was established to discriminate the AMI patients from the controls.RESULTS:In the present study,the expression of plasma miR-1 was signifi cantly increased in the AMI patients compared with the healthy controls(P<0.01). The plasma miR-1 in the AMI patients decreased to the normal level at 14 days(P>0.05). The expression of plasma miR-1 was not related to the clinical characteristics of the study population(P>0.05). ROC curve analyses demonstrated that miR-1 was specifi c and sensitive for the early diagnosis of AMI,but not superior to cTnT.CONCLUSION:Plasma miR-1 could be used in the early diagnosis of AMI,but it is similar to cTnT.展开更多
Abstract： This study investigated the effects of three sport-specific high-intensity training units on cardiac biomarker alteration in elite athletes of dragon boating. Thirty six male （age 33 ± 9） and twenty ...Abstract： This study investigated the effects of three sport-specific high-intensity training units on cardiac biomarker alteration in elite athletes of dragon boating. Thirty six male （age 33 ± 9） and twenty nine female （age 31 ±8） elite athletes, members of the German national team, were examined in their final training camp preparing for world championship. At two time points blood panels （pre-training and one hour post-training） were collected and concentrations of high sensitive troponin T, N-terminal pro brain natriuretic peptide （NT-pro BNP）, ereatine phosphokinase （CPK）, MB-creatine kinase （CKMB） and myoglobin were assessed. After exercise, serum levels ofNT-pro BNP, CPK, myoglobin and CKMB increased significantly （P 〈 0.01 for each） with only few values exceeding the upper reference limits. High sensitive troponin T remained below the limit of detection both before and after exercise in all athletes. Significant gender-related differences were found with a higher increase ofNT-pro BNP levels in female athletes （P 〈 0.01） compared to males. In contrast, male athletes displayed a significant higher increase of CPK （P 〈 0.01） and myoglobin （P 〈 0.01） compared to female athletes. In conclusion, three high-intensity training units did not lead to elevated high sensitive troponin T concentrations in elite athletes of dragon boating but to significant increases ofNT-pro BNP, CPK and myoglobin levels. This suggests that high-intensity training units do not lead to a cardiac injury in these athletes.展开更多
文摘High-sensitivity cardiac troponin(hs-cTn) assays are increasingly being used in many countries worldwide,however,a generally accepted definition of high-sen-sitivity is still pending.These assays enable cTn mea-surement with a high degree of analytical sensitivity with a low analytical imprecision at the low measuring range of cTn assays(coefficient of variation of < 10% at the 99th percentile upper reference limit).One of the most important advantages of these new assays is that they allow novel,more rapid approaches to rule in or rule out acute coronary syndromes(ACSs) than with previous cTn assay generations which are still more commonly used in practice worldwide.hs-cTn is also more sensitive for the detection of myocardial damage unrelated to acute myocardial ischemia.Therefore,the increase in early diagnostic sensitivity of hs-cTn assays for ACS comes at the cost of a reduced ACS specificity,because more patients with other causes of acute or chronic myocardial injury without overt myocardial isch-emia are detected than with previous cTn assays.As hs-cTn assays are increasingly being adopted in clinical practice and more hs-cTn assays are being developed,this review attempts to synthesize the available clinical data to make recommendations for their everyday clini-cal routine use.
基金supported by grants from the National Natural Science Foundation of China(81071030)the Science and Technology Foundation of Guangdong Province(2011B080701006)
文摘BACKGROUND:Early reperfusion can effectively treat acute myocardial infarction(AMI) and reduce the mortality signif icantly. This study aimed to compare the role of plasma microRNA-1(miR-1) and cardiac troponin T(cTnT) in early diagnosis of AMI patients.METHODS:From May 2011 to May 2012,plasma samples were collected from 56 AMI patients and 28 non-AMI controls. The expression of plasma miR-1 was measured by quantitative reverse transcription-polymerase chain reaction(qRT-PCR),and the level of plasma cTnT was measured using electrochemiluminescence-based methods on an Elecsys 2010 Immunoassay Analyzer. SPSS 16.0 was used for the statistical analysis of the results. Data were expressed as mean±standard deviation unless otherwise described. The differences about clinical characteristics between the AMI patients and controls were tested using Student's t test or Fisher's exact test. The Mann-Whitney U test was conducted to compare the expression of microRNAs between the AMI patients and controls. MicroRNAs expression between different intervals of the AMI patients was compared using Wilcoxon's signed-rank test. The receiver operating characteristic(ROC) curve was established to discriminate the AMI patients from the controls.RESULTS:In the present study,the expression of plasma miR-1 was signifi cantly increased in the AMI patients compared with the healthy controls(P<0.01). The plasma miR-1 in the AMI patients decreased to the normal level at 14 days(P>0.05). The expression of plasma miR-1 was not related to the clinical characteristics of the study population(P>0.05). ROC curve analyses demonstrated that miR-1 was specifi c and sensitive for the early diagnosis of AMI,but not superior to cTnT.CONCLUSION:Plasma miR-1 could be used in the early diagnosis of AMI,but it is similar to cTnT.
文摘Abstract： This study investigated the effects of three sport-specific high-intensity training units on cardiac biomarker alteration in elite athletes of dragon boating. Thirty six male （age 33 ± 9） and twenty nine female （age 31 ±8） elite athletes, members of the German national team, were examined in their final training camp preparing for world championship. At two time points blood panels （pre-training and one hour post-training） were collected and concentrations of high sensitive troponin T, N-terminal pro brain natriuretic peptide （NT-pro BNP）, ereatine phosphokinase （CPK）, MB-creatine kinase （CKMB） and myoglobin were assessed. After exercise, serum levels ofNT-pro BNP, CPK, myoglobin and CKMB increased significantly （P 〈 0.01 for each） with only few values exceeding the upper reference limits. High sensitive troponin T remained below the limit of detection both before and after exercise in all athletes. Significant gender-related differences were found with a higher increase ofNT-pro BNP levels in female athletes （P 〈 0.01） compared to males. In contrast, male athletes displayed a significant higher increase of CPK （P 〈 0.01） and myoglobin （P 〈 0.01） compared to female athletes. In conclusion, three high-intensity training units did not lead to elevated high sensitive troponin T concentrations in elite athletes of dragon boating but to significant increases ofNT-pro BNP, CPK and myoglobin levels. This suggests that high-intensity training units do not lead to a cardiac injury in these athletes.
