Background:Depression is a common clinical phenomenon in the patients with heart failure(HF).In traditional Chinese medicine(TCM),diseases in the brain and heart are thought to be correlated and interact.Naoxintong ca...Background:Depression is a common clinical phenomenon in the patients with heart failure(HF).In traditional Chinese medicine(TCM),diseases in the brain and heart are thought to be correlated and interact.Naoxintong capsules(NXT)has been used for treating cardio-cerebrovascular diseases,while its therapeutic effect on depression after HF remains unclear.Objective:The aim of the study is to evaluate the intervention effect of NXT on depression after HF.Methods:Sprague-Dawley rats were assigned into the following 5 groups:sham,model,NXT(250,1000 mg/kg),and valsartan(8mg/kg).Coronary artery occlusion was performed to induce HF and subsequent depression in rats.The cardiac function was evaluated by echocardiography,hematoxylin-eosin staining,and Masson trichrome staining.The sucrose preference test and Morris water maze test were carried out to assess the depressive behaviors in rats.The ultrastructure of hippocampal CA1 neurons was observed and the levels of corticotropin-releasing hormone in the hypothalamus,brain-derived neurotrophic factor in the cortex,and adrenocorticotropic hormone(ACTH)in the plasma were determined by enzyme-linked immunosorbent assay.The levels of dopamine,5-hydroxytryptamine,norepinephrine,andγ-aminobutyric acid in the hippocampus were measured by UPLC-QQQ-MS.Results:NXT reduced myocardial injury and pathological changes in the cardiac tissue and increased the left ventricular ejection fraction,left ventricular fractional shortening,and cardiac output.NXT increased the sugar preference rate and number of crossings and shortened the escape latency.Furthermore,the NXT treatment restored the levels of corticotropin-releasing hormone,adrenocorticotropic hormone,brain-derived neurotrophic factor,dopamine,andγ-aminobutyric acid to the baseline values.Conclusions:NXT not only demonstrates cardioprotective effect but also attenuates depression in the rats after HF.It may exert the antidepressant effect by inhibiting the hyperactivity of the hypothalamic-pituitary-adrenal axis and recovering the levels of neurotrophic factors and neurotransmitters.展开更多
The antioxidant activity and its protective effects of Green Tea Extract (GTE) from a Chinese green tea, Hangzhou Longjing, on acute myocardial inthrction (AMI) were investigated in rats. Blood, liver and heart we...The antioxidant activity and its protective effects of Green Tea Extract (GTE) from a Chinese green tea, Hangzhou Longjing, on acute myocardial inthrction (AMI) were investigated in rats. Blood, liver and heart were collected to perform DNA damage test, hepatic antioxidant test, gene expression as well as capillary distribution study. A well-known angiotensin II type I receptor antagonist, Losartan was used as control. It was fbund there were significant reductions in certain DNA damage products' levels in GTE and Losartan treated groups compared to saline-treated group. In addition, significant increases in antioxidant enzyme activities and antioxidant gene expressions were observed in GTE and Losartan treated groups. Molphological study also demonstrated the cardioprotective effects of GTE on AMI. In conclusion, GTE has potent and similar cardioprotective effect as Losartan that mav due to its ability to scavenge free radicals.展开更多
基金supported by Scientific and Technological Innovation Project of China Academy of Chinese Medical Sciences(No.CI2021B017-07,CI2021A05208,CI2021A04202)National Natural Science Foundation of China(No.81973711).
文摘Background:Depression is a common clinical phenomenon in the patients with heart failure(HF).In traditional Chinese medicine(TCM),diseases in the brain and heart are thought to be correlated and interact.Naoxintong capsules(NXT)has been used for treating cardio-cerebrovascular diseases,while its therapeutic effect on depression after HF remains unclear.Objective:The aim of the study is to evaluate the intervention effect of NXT on depression after HF.Methods:Sprague-Dawley rats were assigned into the following 5 groups:sham,model,NXT(250,1000 mg/kg),and valsartan(8mg/kg).Coronary artery occlusion was performed to induce HF and subsequent depression in rats.The cardiac function was evaluated by echocardiography,hematoxylin-eosin staining,and Masson trichrome staining.The sucrose preference test and Morris water maze test were carried out to assess the depressive behaviors in rats.The ultrastructure of hippocampal CA1 neurons was observed and the levels of corticotropin-releasing hormone in the hypothalamus,brain-derived neurotrophic factor in the cortex,and adrenocorticotropic hormone(ACTH)in the plasma were determined by enzyme-linked immunosorbent assay.The levels of dopamine,5-hydroxytryptamine,norepinephrine,andγ-aminobutyric acid in the hippocampus were measured by UPLC-QQQ-MS.Results:NXT reduced myocardial injury and pathological changes in the cardiac tissue and increased the left ventricular ejection fraction,left ventricular fractional shortening,and cardiac output.NXT increased the sugar preference rate and number of crossings and shortened the escape latency.Furthermore,the NXT treatment restored the levels of corticotropin-releasing hormone,adrenocorticotropic hormone,brain-derived neurotrophic factor,dopamine,andγ-aminobutyric acid to the baseline values.Conclusions:NXT not only demonstrates cardioprotective effect but also attenuates depression in the rats after HF.It may exert the antidepressant effect by inhibiting the hyperactivity of the hypothalamic-pituitary-adrenal axis and recovering the levels of neurotrophic factors and neurotransmitters.
基金Ministry of Science and Technology of China (Grant No. 2012ZX09103-301-033)the Fundamental Research Funds for the Central Universities (Grant No. 11611206)
文摘The antioxidant activity and its protective effects of Green Tea Extract (GTE) from a Chinese green tea, Hangzhou Longjing, on acute myocardial inthrction (AMI) were investigated in rats. Blood, liver and heart were collected to perform DNA damage test, hepatic antioxidant test, gene expression as well as capillary distribution study. A well-known angiotensin II type I receptor antagonist, Losartan was used as control. It was fbund there were significant reductions in certain DNA damage products' levels in GTE and Losartan treated groups compared to saline-treated group. In addition, significant increases in antioxidant enzyme activities and antioxidant gene expressions were observed in GTE and Losartan treated groups. Molphological study also demonstrated the cardioprotective effects of GTE on AMI. In conclusion, GTE has potent and similar cardioprotective effect as Losartan that mav due to its ability to scavenge free radicals.