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柴葛芩连汤对解脲脲原体肺炎小鼠肺功能及CARDS TX/NLRP3信号通路的影响
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作者 黄方 许诣 +4 位作者 杨雪 汪枝繁 王静 沈文婷 陈霞 《中成药》 CAS CSCD 北大核心 2023年第8期2537-2542,共6页
目的探究柴葛芩连汤对解脲脲原体肺炎模型小鼠肺功能以及CARDS TX/NLRP3信号通路的影响。方法60只小鼠分为对照组,肺炎组,柴葛芩连汤低、高剂量组和阿奇霉素组。鼻腔滴注解脲脲原体菌液进行造模,柴葛芩连汤低、高剂量组灌胃5.4、16.2 g... 目的探究柴葛芩连汤对解脲脲原体肺炎模型小鼠肺功能以及CARDS TX/NLRP3信号通路的影响。方法60只小鼠分为对照组,肺炎组,柴葛芩连汤低、高剂量组和阿奇霉素组。鼻腔滴注解脲脲原体菌液进行造模,柴葛芩连汤低、高剂量组灌胃5.4、16.2 g/kg柴葛芩连汤;阿奇霉素组灌胃90 mg/kg阿奇霉素溶液2个周期(1周期为7 d,前3 d连续灌胃阿奇霉素溶液,后4 d灌胃等量蒸馏水);对照组和肺炎组灌胃等量蒸馏水,连续14 d。采用动物肺功能分析仪检测肺功能,ELISA法检测肺泡灌洗液中炎症因子水平,HE染色观察肺组织病理变化,RT-qPCR和Western blot法检测肺组织肺炎支原体外毒素(CARDS TX)、炎症小体(NLRP3)、凋亡相关斑点样蛋白(ASC)、半胱氨酸天冬氨酸蛋白酶-1(caspase-1)的mRNA和蛋白表达。结果与对照组比较,肺炎组小鼠PEF、FEV0.1、FEF-50降低(P<0.05),IL-6、TNF-α水平及CARDS TX、NLRP3、ASC、caspase-1 mRNA和蛋白表达均升高(P<0.05),肺泡结构模糊,有炎性细胞浸润;与肺炎组比较,柴葛芩连汤各剂量组和阿奇霉素组小鼠PEF、FEV0.1与FEF-50升高(P<0.05),IL-6、TNF-α水平及CARDS TX、NLRP3、ASC、caspase-1 mRNA和蛋白表达均降低(P<0.05),肺组织病理变化有不同程度缓解。结论柴葛芩连汤可以改善解脲脲原体肺炎小鼠受损的肺功能,该作用可能与抑制CARDS TX/NLRP3信号通路有关。 展开更多
关键词 柴葛芩连汤 肺炎 解脲脲原体 cards TX NLRP3
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基于多交叉置换扩增和纳米生物传感技术快速检测肺炎支原体方法的建立
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作者 肖飞 郑宝英 +8 位作者 徐文健 伏瑾 黄小兰 孙春荣 贾楠 张裕 许峥 周娟 王毅 《遵义医科大学学报》 2024年第5期513-521,共9页
目的建立一种简单、灵敏、快速的肺炎支原体(MP)检测方法,并对其应用性进行验证和评价。方法利用多交叉置换扩增(MCDA)技术对肺炎支原体特异基因CARDS毒素基因进行扩增,利用侧流免疫层析生物传感(LFB)技术读取扩增结果,命名该方法为MP-M... 目的建立一种简单、灵敏、快速的肺炎支原体(MP)检测方法,并对其应用性进行验证和评价。方法利用多交叉置换扩增(MCDA)技术对肺炎支原体特异基因CARDS毒素基因进行扩增,利用侧流免疫层析生物传感(LFB)技术读取扩增结果,命名该方法为MP-MCDA-LFB。分析扩增反应在60~67℃(间隔1℃)的扩增效率,筛选最适反应温度;分析分别扩增10、20、30、40 min时能够检测到的最低核酸浓度,筛选最佳反应时间。利用10倍系列稀释的肺炎支原体核酸分析MP-MCDA-LFB方法的灵敏度和检测限,利用35株非肺炎支原体菌株分析MP-MCDA-LFB方法的特异性。利用MP-MCDA-LFB方法检测80份疑似MP感染的临床样本,并与RT-PCR法检测结果进行比较,分析MP-MCDA-LFB方法的临床应用性。结果MP-MCDA-LFB能够实现对肺炎支原体CARDS毒素基因的快速检测。其最佳反应温度为63℃,最短反应时间为40 min,整个检测过程可在1 h内。MP-MCDA-LFB方法具有较高的灵敏度和特异性,其检测限低至45 ng/L,与其他临床表现相似的病原体无交叉反应,特异性为100%。MP-MCDA-LFB方法从80份临床样本中检出45份阳性样本(56.3%),检出率与RT-PCR方法一致。结论本研究建立的以CARDS毒素基因为靶标的MP-MCDA-LFB检测方法具有简单、快速、灵敏度高、特异性强的优点,在基层医疗机构和现场检测具有较好的应用潜力。 展开更多
关键词 肺炎支原体 多交叉置换扩增技术 侧流免疫层析生物传感技术 cards毒素基因 RT-PCR
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肺炎支原体CARDS毒素蛋白多表位拼接抗原的表达及鉴定 被引量:3
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作者 刘宝山 赵芝娜 +1 位作者 赵雨杰 王桂珍 《中国人兽共患病学报》 CAS CSCD 北大核心 2017年第7期588-591,共4页
目的探讨肺炎支原体CARDS毒素蛋白多表位拼接抗原的免疫反应活性。方法对CARDS毒素蛋白的抗原表位进行分析,选取10个重要的表位进行拼接,反向翻译后合成并克隆,插入pET28a表达载体构建pET-CARDS表达质粒并转入受体菌。经IPTG诱导表达的... 目的探讨肺炎支原体CARDS毒素蛋白多表位拼接抗原的免疫反应活性。方法对CARDS毒素蛋白的抗原表位进行分析,选取10个重要的表位进行拼接,反向翻译后合成并克隆,插入pET28a表达载体构建pET-CARDS表达质粒并转入受体菌。经IPTG诱导表达的多表位拼接CARDS蛋白使用6×His单克隆抗体和人阳性血清进行了免疫印迹的检测。结果 CARDS毒素蛋白的多表位拼接抗原表达载体构建成功,诱导后表达大小为30KDa的重组蛋白,Western blot测定其能与6×His单克隆抗体和人阳性血清发生反应。结论本研究选取的CARDS毒素蛋白抗原表位具有较强的免疫活性,可为Mp感染的诊断提供新的候选抗原。 展开更多
关键词 肺炎支原体 cards毒素蛋白 多表位拼接抗原 表达 鉴定
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CARD6在脓毒症心肌损伤中的机制研究
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作者 李一民 石宁宁 +2 位作者 曲央旺姆 辛勇 周吴刚 《上海医药》 CAS 2024年第5期39-42,69,共5页
目的:探讨CARD6在脓毒症心肌损伤中的调节机制。方法:将C57BL/6小鼠随机分为对照组与模型组。采用LPS腹腔注射法构建脓毒症心肌损伤小鼠模型,RT-qPCR检测小鼠心肌组织中CARD6、caspase-1的mRNA水平。体外实验则采用LPS刺激转染CARD6过... 目的:探讨CARD6在脓毒症心肌损伤中的调节机制。方法:将C57BL/6小鼠随机分为对照组与模型组。采用LPS腹腔注射法构建脓毒症心肌损伤小鼠模型,RT-qPCR检测小鼠心肌组织中CARD6、caspase-1的mRNA水平。体外实验则采用LPS刺激转染CARD6过表达质粒的H9C2细胞,检测caspase-1、细胞活力、CK-MB水平。结果:与对照组相比,模型组小鼠心肌组织中LDH、CK-MB含量,caspase-1表达水平上升,提示脓毒症导致小鼠心肌损伤,而CARD6表达水平明显降低。体外实验发现CARD6表达水平呈下降趋势,转染含有CARD6的过表达质粒后细胞释放CK-MB的水平降低、细胞活力增加、caspase-1水平降低。结论:CARD6可改善脓毒症所致心肌损伤,其机制可能与caspase-1介导的细胞焦亡相关。 展开更多
关键词 CARD6 CASPASE-1 细胞焦亡 脓毒症 心肌损伤
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综合控制糖尿病患者心血管疾病的多重危险因素——从UKPDS到CARDS 被引量:1
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作者 胡大一 《中国医刊》 CAS 2004年第7期59-59,共1页
关键词 综合控制 糖尿病 心血管疾病 危险因素 UKPDS cards
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Working condition recognition of sucker rod pumping system based on 4-segment time-frequency signature matrix and deep learning
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作者 Yun-Peng He Hai-Bo Cheng +4 位作者 Peng Zeng Chuan-Zhi Zang Qing-Wei Dong Guang-Xi Wan Xiao-Ting Dong 《Petroleum Science》 SCIE EI CAS CSCD 2024年第1期641-653,共13页
High-precision and real-time diagnosis of sucker rod pumping system(SRPS)is important for quickly mastering oil well operations.Deep learning-based method for classifying the dynamometer card(DC)of oil wells is an eff... High-precision and real-time diagnosis of sucker rod pumping system(SRPS)is important for quickly mastering oil well operations.Deep learning-based method for classifying the dynamometer card(DC)of oil wells is an efficient diagnosis method.However,the input of the DC as a two-dimensional image into the deep learning framework suffers from low feature utilization and high computational effort.Additionally,different SRPSs in an oil field have various system parameters,and the same SRPS generates different DCs at different moments.Thus,there is heterogeneity in field data,which can dramatically impair the diagnostic accuracy.To solve the above problems,a working condition recognition method based on 4-segment time-frequency signature matrix(4S-TFSM)and deep learning is presented in this paper.First,the 4-segment time-frequency signature(4S-TFS)method that can reduce the computing power requirements is proposed for feature extraction of DC data.Subsequently,the 4S-TFSM is constructed by relative normalization and matrix calculation to synthesize the features of multiple data and solve the problem of data heterogeneity.Finally,a convolutional neural network(CNN),one of the deep learning frameworks,is used to determine the functioning conditions based on the 4S-TFSM.Experiments on field data verify that the proposed diagnostic method based on 4S-TFSM and CNN(4S-TFSM-CNN)can significantly improve the accuracy of working condition recognition with lower computational cost.To the best of our knowledge,this is the first work to discuss the effect of data heterogeneity on the working condition recognition performance of SRPS. 展开更多
关键词 Sucker-rod pumping system Dynamometer card Working condition recognition Deep learning Time-frequency signature Time-frequency signature matrix
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A Review of Lightweight Security and Privacy for Resource-Constrained IoT Devices
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作者 Sunil Kumar Dilip Kumar +3 位作者 Ramraj Dangi Gaurav Choudhary Nicola Dragoni Ilsun You 《Computers, Materials & Continua》 SCIE EI 2024年第1期31-63,共33页
The widespread and growing interest in the Internet of Things(IoT)may be attributed to its usefulness in many different fields.Physical settings are probed for data,which is then transferred via linked networks.There ... The widespread and growing interest in the Internet of Things(IoT)may be attributed to its usefulness in many different fields.Physical settings are probed for data,which is then transferred via linked networks.There are several hurdles to overcome when putting IoT into practice,from managing server infrastructure to coordinating the use of tiny sensors.When it comes to deploying IoT,everyone agrees that security is the biggest issue.This is due to the fact that a large number of IoT devices exist in the physicalworld and thatmany of themhave constrained resources such as electricity,memory,processing power,and square footage.This research intends to analyse resource-constrained IoT devices,including RFID tags,sensors,and smart cards,and the issues involved with protecting them in such restricted circumstances.Using lightweight cryptography,the information sent between these gadgets may be secured.In order to provide a holistic picture,this research evaluates and contrasts well-known algorithms based on their implementation cost,hardware/software efficiency,and attack resistance features.We also emphasised how essential lightweight encryption is for striking a good cost-to-performance-to-security ratio. 展开更多
关键词 IOT a sensor device LIGHTWEIGHT CRYPTOGRAPHY block cipher smart card security and privacy
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Credit Card Fraud Detection Using Improved Deep Learning Models
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作者 Sumaya S.Sulaiman Ibraheem Nadher Sarab M.Hameed 《Computers, Materials & Continua》 SCIE EI 2024年第1期1049-1069,共21页
Fraud of credit cards is a major issue for financial organizations and individuals.As fraudulent actions become more complex,a demand for better fraud detection systems is rising.Deep learning approaches have shown pr... Fraud of credit cards is a major issue for financial organizations and individuals.As fraudulent actions become more complex,a demand for better fraud detection systems is rising.Deep learning approaches have shown promise in several fields,including detecting credit card fraud.However,the efficacy of these models is heavily dependent on the careful selection of appropriate hyperparameters.This paper introduces models that integrate deep learning models with hyperparameter tuning techniques to learn the patterns and relationships within credit card transaction data,thereby improving fraud detection.Three deep learning models:AutoEncoder(AE),Convolution Neural Network(CNN),and Long Short-Term Memory(LSTM)are proposed to investigate how hyperparameter adjustment impacts the efficacy of deep learning models used to identify credit card fraud.The experiments conducted on a European credit card fraud dataset using different hyperparameters and three deep learning models demonstrate that the proposed models achieve a tradeoff between detection rate and precision,leading these models to be effective in accurately predicting credit card fraud.The results demonstrate that LSTM significantly outperformed AE and CNN in terms of accuracy(99.2%),detection rate(93.3%),and area under the curve(96.3%).These proposed models have surpassed those of existing studies and are expected to make a significant contribution to the field of credit card fraud detection. 展开更多
关键词 Card fraud detection hyperparameter tuning deep learning autoencoder convolution neural network long short-term memory RESAMPLING
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A Self-Adapting and Efficient Dandelion Algorithm and Its Application to Feature Selection for Credit Card Fraud Detection
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作者 Honghao Zhu MengChu Zhou +1 位作者 Yu Xie Aiiad Albeshri 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2024年第2期377-390,共14页
A dandelion algorithm(DA) is a recently developed intelligent optimization algorithm for function optimization problems. Many of its parameters need to be set by experience in DA,which might not be appropriate for all... A dandelion algorithm(DA) is a recently developed intelligent optimization algorithm for function optimization problems. Many of its parameters need to be set by experience in DA,which might not be appropriate for all optimization problems. A self-adapting and efficient dandelion algorithm is proposed in this work to lower the number of DA's parameters and simplify DA's structure. Only the normal sowing operator is retained;while the other operators are discarded. An adaptive seeding radius strategy is designed for the core dandelion. The results show that the proposed algorithm achieves better performance on the standard test functions with less time consumption than its competitive peers. In addition, the proposed algorithm is applied to feature selection for credit card fraud detection(CCFD), and the results indicate that it can obtain higher classification and detection performance than the-state-of-the-art methods. 展开更多
关键词 Credit card fraud detection(CCFD) dandelion algorithm(DA) feature selection normal sowing operator
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CARD11 serves as a therapeutic biomarker for the drug therapies of ccRCC
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作者 KAIWEN TIAN HANZHONG CHEN +6 位作者 QIANQIAN WANG FENGLIAN JIANG CHUNXIANG FENG TENG LI XIAOYONG PU YANLIN TANG JIUMIN LIU 《BIOCELL》 SCIE 2024年第5期817-834,共18页
Background:The incidence of clear cell renal cell carcinoma(ccRCC)is globally high;however,despite the introduction of innovative drug therapies,there remains a lack of effective biomarkers for evaluating treatment re... Background:The incidence of clear cell renal cell carcinoma(ccRCC)is globally high;however,despite the introduction of innovative drug therapies,there remains a lack of effective biomarkers for evaluating treatment response.Recently,Caspase recruiting domain-containing protein 11(CARD11)has garnered attention due to its significant association with tumor development and the immune system.Methods:The expression of CARD11 mRNA and protein in ccRCC were analyzed by public database and immunohistochemistry.The focus of this study is on the epigenomic modifications of CARD11,its expression of ccRCC immunophenotype,and its correlation with response to immunotherapy and targeted therapy.Furthermore,to investigate the mechanism of this molecule’s influence on different biological behaviors of cells,cell tests in vitro have been conducted to observe the impact of its expression level.Results:CARD11 expression was upregulated which may be mainly modified by body methylation and was correlated with poor prognosis in ccRCC.In the tumor microenvironment of ccRCC,CARD11 expression was positively correlated with increased T lymphocyte infiltration and increased expression of inhibitory immune checkpoints.Moreover,ccRCC patients with high CARD11 expression had a better response to immunotherapy and targeted therapy.The knockdown of CARD11 ultimately suppressed the proliferation,migration,and invasion capabilities of ccRCC cells while simultaneously enhancing tumor cell apoptosis.Conclusion:We identified CARD11 as a novel therapeutic biomarker for immunotherapy and targeted therapy in ccRCC. 展开更多
关键词 Clear cell renal cell carcinoma Tumor microenvironment CARD11 Immune checkpoint inhibitor Tyrosine kinase inhibitor
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退变性腰椎滑脱French分型和CARDS分型的可重复性与可信度研究 被引量:13
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作者 盛亚超 孙旭 +5 位作者 朱泽章 史本龙 孙伟翔 陈曦 袁鑫鑫 邱勇 《中国脊柱脊髓杂志》 CAS CSCD 北大核心 2017年第3期228-234,共7页
目的 :对退变性腰椎滑脱的French分型及CARDS分型进行可重复性与可信度的对比分析,探讨两种分型在退变性腰椎滑脱患者中的应用价值。方法:回顾性分析2012年1月~2016年6月期间118例腰椎退变性滑脱(L4/5 91例、L5/S1 27例)患者,其中男性26... 目的 :对退变性腰椎滑脱的French分型及CARDS分型进行可重复性与可信度的对比分析,探讨两种分型在退变性腰椎滑脱患者中的应用价值。方法:回顾性分析2012年1月~2016年6月期间118例腰椎退变性滑脱(L4/5 91例、L5/S1 27例)患者,其中男性26例,女性92例,平均年龄61.1±8.1岁。3名脊柱外科医师对患者术前X线片独自进行两次测量,分别使用French分型和临床与影像学分型(clinical and radiographic degenerative spondylolisthesis,CARDS分型)进行评估和分型,收集结果 ,作同一观察者间可重复性及不同观察者间可信度分析。应用Kappa值比较分析两种分型的差异性。结果:3位观察者使用French分型系统共进行708次(118例×3×2次)分型,包括1型261次,2型107次,3型83次,4型54次,5型203次,观察者内分型一致率80.5%~86.4%(Kappa值0.740~0.815),属于"基本可信";观察者间分型一致率为79.7%~82.2%(Kappa值0.728~0.758),属于"基本可信"。测量并分型单个患者平均花费时间约138s。CARDS分型系统共708次分型中,包括A型(A1)19次,B型(B1 90次,B2 59次)149次,C型(C1 291次,C2 108次)399次,D型(D1 98次,D2 43次)141次,观察者内总体一致率90.7%~93.2%(Kappa值0.878~0.911),属于"完全可信";观察者间总体一致率88.1%~94.1%(Kappa值0.844~0.921),属于"完全可信"。测量并分型单个患者平均花费时间约67s。结论 :两种分型系统具有较高的可重复性与可信度,CARDS分型可信度与可重复性优于French分型。 展开更多
关键词 退变性滑脱 French分型 cards分型 可信度 可重复性
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Real-Time Fraud Detection Using Machine Learning
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作者 Benjamin Borketey 《Journal of Data Analysis and Information Processing》 2024年第2期189-209,共21页
Credit card fraud remains a significant challenge, with financial losses and consumer protection at stake. This study addresses the need for practical, real-time fraud detection methodologies. Using a Kaggle credit ca... Credit card fraud remains a significant challenge, with financial losses and consumer protection at stake. This study addresses the need for practical, real-time fraud detection methodologies. Using a Kaggle credit card dataset, I tackle class imbalance using the Synthetic Minority Oversampling Technique (SMOTE) to enhance modeling efficiency. I compare several machine learning algorithms, including Logistic Regression, Linear Discriminant Analysis, K-nearest Neighbors, Classification and Regression Tree, Naive Bayes, Support Vector, Random Forest, XGBoost, and Light Gradient-Boosting Machine to classify transactions as fraud or genuine. Rigorous evaluation metrics, such as AUC, PRAUC, F1, KS, Recall, and Precision, identify the Random Forest as the best performer in detecting fraudulent activities. The Random Forest model successfully identifies approximately 92% of transactions scoring 90 and above as fraudulent, equating to a detection rate of over 70% for all fraudulent transactions in the test dataset. Moreover, the model captures more than half of the fraud in each bin of the test dataset. SHAP values provide model explainability, with the SHAP summary plot highlighting the global importance of individual features, such as “V12” and “V14”. SHAP force plots offer local interpretability, revealing the impact of specific features on individual predictions. This study demonstrates the potential of machine learning, particularly the Random Forest model, for real-time credit card fraud detection, offering a promising approach to mitigate financial losses and protect consumers. 展开更多
关键词 Credit Card Fraud Detection Machine Learning SHAP Values Random Forest
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清肺透邪汤对肺炎支原体感染小鼠CARDS TX/NLRP3炎性小体相关因子表达的影响 被引量:12
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作者 王子 王雪峰 +2 位作者 张秀英 吴振起 张童 《中华中医药学刊》 CAS 北大核心 2020年第7期121-126,I0017,共7页
目的探讨清肺透邪汤对肺炎支原体(MP)感染后CARDS TX及NLRP3炎性小体相关因子的变化及相关性,试图进一步明确清肺透邪汤防治肺炎支原体肺炎(MPP)的效应靶点。方法随机将48只Balb/c幼鼠分成正常组、模型组、清肺透邪汤组、阿奇霉素组4组... 目的探讨清肺透邪汤对肺炎支原体(MP)感染后CARDS TX及NLRP3炎性小体相关因子的变化及相关性,试图进一步明确清肺透邪汤防治肺炎支原体肺炎(MPP)的效应靶点。方法随机将48只Balb/c幼鼠分成正常组、模型组、清肺透邪汤组、阿奇霉素组4组,每组12只。除正常组外,对其余3组Balb/c幼鼠采用滴鼻法进行MP感染。造模后,清肺透邪汤组每只幼鼠给予15 g/kg的对应方剂进行灌胃,1次/d,连续14 d;阿奇霉素组,每只幼鼠给予阿奇霉素90 mg/kg灌胃,1次/d,连续3 d,停药4 d,2个周期,停药期间给予蒸馏水灌胃;正常组、模型组每只幼鼠均给予0.3 mL蒸馏水灌胃。在感染后的第3、7、10、14天进行取材,运用Real-time PCR法检测肺组织CARDS TX、NLRP3、ASC及Caspase-1的表达,采用ELISA法检测血清中IL-1β表达水平,采用免疫组化法检测肺组织中NF-κB表达。结果MP感染后,幼鼠肺组织出现炎症改变,镜下可见肺泡壁增厚、肺泡上皮细胞破坏、浸润。MP感染后,幼鼠肺组织中的CARDS TX、NLRP3、ASC及Caspase-1基因表达明显升高;与模型相比,清肺透邪方组幼鼠肺组织中的CARDS TX、NLRP3、ASC及Caspase-1基因表达明显下降,其上游调控因子NF-κB减少,下游促炎因子IL-1β降低(均P<0.05)。结论清肺透邪汤可以有效地下调CARDS TX基因表达,降低MP毒力作用的主要效应靶点;初步证实了清肺透邪汤可以通过NF-κB通路调控,影响NLRP3炎性小体合成,促使IL-1β所致炎性反应减弱,可能是保护肺损伤的相关机制。 展开更多
关键词 肺炎支原体 清肺透邪汤 NLRP3 cards TX 小鼠
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Leveraging Zibo Barbecue’s Success to Develop Hebei’s Culinary Brand:A Strategic Path
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作者 Chengguo E Yang Yang +1 位作者 Ali Mao Duo Pan 《Proceedings of Business and Economic Studies》 2024年第3期48-52,共5页
In 2023,Zibo barbecue culture exploded across the entire country,allowing people nationwide to experience and enjoy Zibo barbecue.This phenomenon injected new vitality into the economic development of Zibo.To promote ... In 2023,Zibo barbecue culture exploded across the entire country,allowing people nationwide to experience and enjoy Zibo barbecue.This phenomenon injected new vitality into the economic development of Zibo.To promote the economic development of Hebei Province,this paper fully analyzes the reasons behind the popularity of Zibo barbecue,combines these insights with the characteristics of traditional cuisine in Hebei,and draws lessons from Zibo barbecue’s success as a business card for Zibo.The paper then outlines a strategy for building a culinary business card for Hebei. 展开更多
关键词 Zibo barbecue Hebei traditional food Online platform City business card Government-enterprise cooperation
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退变性腰椎滑脱CARDS分型中各亚组患者在脊柱骨盆矢状位参数上的差异 被引量:1
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作者 李宗欢 刘金龙 +3 位作者 耿伟 张野 李洁 马金柱 《中国现代医生》 2021年第5期57-60,65,共5页
目的探讨退变性腰椎滑脱临床与影像学(CARDS)分型中各亚组患者在脊柱骨盆矢状位参数上的差异。方法回顾性分析2018年1月至2019年12月我院收治的92例L4~5单阶段退变性腰椎滑脱患者的临床资料。依据腰椎正侧位X线或动力位腰椎X线正侧位上... 目的探讨退变性腰椎滑脱临床与影像学(CARDS)分型中各亚组患者在脊柱骨盆矢状位参数上的差异。方法回顾性分析2018年1月至2019年12月我院收治的92例L4~5单阶段退变性腰椎滑脱患者的临床资料。依据腰椎正侧位X线或动力位腰椎X线正侧位上滑脱节段椎间隙是否后凸成角分为D型滑脱组和非D型滑脱组(即A、B、C三型)。术前、术后均测量入组患者的脊柱骨盆矢状位参数,同时采用Oswestry功能障碍指数(ODI)和腰部疼痛视觉模拟评分法(VAS)对患者生活质量进行评估。结果术前D型滑脱组LL、PI、SS均显著低于非D型滑脱组,而PT显著高于非D型滑脱组,差异有统计学意义(P<0.05)。术前D型滑脱组ODI评分和腰痛VAS评分均显著高于非D型滑脱组,差异有统计学意义(P<0.05)。结论退变性腰椎滑脱CARDS分型中D型患者与非D型患者在脊柱骨盆矢状位参数存在差异,D型可以作为一种独特的亚型进行探讨,可以推广为临床常用的术前评估分类系统之一。D型患者术前腰椎生理前凸丢失,容易表现出更严重的临床症状。 展开更多
关键词 退变性腰椎滑脱 cards分型 脊柱骨盆矢状位参数
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Christmas Cards
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作者 李国勤 《疯狂英语(初中天地)》 2002年第43期26-26,共1页
Every December,millions of Christmas cards go through the post offices.There're pictures of stars, snow or Christmas tree on the cards. In these days it's hard to think of a Christmas without cards,yet
关键词 Christmas cards
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Christmas Cards
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作者 李国勤 《疯狂英语(初中天地)》 2002年第47期26-26,共1页
Every December,millions of Christmascards go through thepost offices.There’repictures of stars。
关键词 Christmas cards
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社区获得性呼吸窘迫综合征(CARDS)肺炎支原体毒素促进THP-1细胞自噬并激活NLRP3炎性体 被引量:10
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作者 夏雯 戴晓玥 +5 位作者 吴亮 易承学 邹治情 丁龙坤 席月 许化溪 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2020年第12期1076-1082,共7页
目的研究社区获得性呼吸窘迫综合征(CARDS)肺炎支原体(Mp)毒素诱导THP-1细胞自噬以及活化含pyrin结构域核苷酸结合寡聚结构域样受体家族3(NLRP3)炎性体的能力和机制。方法采用原核表达技术获得重组CARDS(rCARDS)Mp毒素,5μg/mL和10μg/m... 目的研究社区获得性呼吸窘迫综合征(CARDS)肺炎支原体(Mp)毒素诱导THP-1细胞自噬以及活化含pyrin结构域核苷酸结合寡聚结构域样受体家族3(NLRP3)炎性体的能力和机制。方法采用原核表达技术获得重组CARDS(rCARDS)Mp毒素,5μg/mL和10μg/mL rCARDS Mp毒素作用THP-1细胞20 min、40 min、1 h、2 h和3 h。Western blot法检测THP-1细胞自噬相关蛋白beclin-1、微管相关蛋白1轻链3Ⅱ(LC3Ⅱ)和P62表达水平,实时定量PCR检测THP-1细胞NLRP3、胱天蛋白酶1(caspase-1)和白细胞介素1β(IL-1β)的mRNA水平,2',7'-二氯二氢荧光素二乙酸酯染色检测THP-1细胞活性氧(ROS)水平。结果与正常细胞对照组相比,rCARDS毒素作用1 h内,THP-1细胞beclin-1、LC3和P62表达量随着作用时间延长明显增加;rCARDS毒素作用2 h和3 h时,beclin-1、LC3和P62表达量降低。rCARDS Mp毒素作用THP-1细胞20 min和40 min时,5μg/mL、10μg/mL rCARDS Mp毒素组细胞NLRP3表达量无显著差别,但在各时间点,rCARDS Mp毒素处理的THP-1细胞NLRP3 mRNA水平均显著高于正常对照组,且作用1 h和2 h时,10μg/mL组细胞NLRP3 mRNA水平显著高于5μg/mL组,而3 h时5μg/mL组和10μg/mL组细胞NLRP3 mRNA水平均低于2 h时。rCARDS Mp毒素作用40 min、1 h和2 h时,不同剂量rCARDS Mp毒素组细胞caspase-1 mRNA水平均高于正常细胞对照组,作用40 min、1 h、2 h和3 h时,10μg/mL组细胞caspase-1 mRNA水平均显著高于5μg/mL组。与正常细胞对照组相比,rCARDS Mp毒素作用20 min和40 min时,不同剂量rCARDS Mp毒素组细胞IL-1βmRNA水平变化不明显,rCARDS Mp毒素作用1 h^3 h时,各组细胞IL-1βmRNA和ROS水平显著增加并呈剂量依赖性。结论CARDS肺炎支原体毒素可以激活THP-1细胞NLRP3炎性体活化并诱导细胞自噬。 展开更多
关键词 肺炎支原体毒素 社区获得性呼吸窘迫综合征(cards) 自噬 含pyrin结构域核苷酸结合寡聚结构域样受体家族3(NLRP3)
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A new modified remote user authentication scheme using smart cards 被引量:4
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作者 SHEN Zhong-hua 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2008年第3期371-376,共6页
In 2000, a remote user authentication scheme using smart cards was proposed and the masquerade attacks were proved successful on this scheme. Recently, Kumar has suggested the idea of check digits to overcome the abov... In 2000, a remote user authentication scheme using smart cards was proposed and the masquerade attacks were proved successful on this scheme. Recently, Kumar has suggested the idea of check digits to overcome the above attacks with a new scheme that removes these threats well. In this paper it is pointed out that the weakness still exists in Kumar's scheme, and the intruder can login to the remote system through having some information. A new scheme which can overcome these attacks and appears more secure and efficient than Kumar's is presented. 展开更多
关键词 remote user AUTHENTICATION smart card CRYPTOGRAPHY
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Progressive Randomization of a Deck of Playing Cards: Experimental Tests and Statistical Analysis of the Riffle Shuffle 被引量:1
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作者 M. P. Silverman 《Open Journal of Statistics》 2019年第2期268-298,共31页
The question of how many shuffles are required to randomize an initially ordered deck of cards is a problem that has fascinated mathematicians, scientists, and the general public. The two principal theoretical approac... The question of how many shuffles are required to randomize an initially ordered deck of cards is a problem that has fascinated mathematicians, scientists, and the general public. The two principal theoretical approaches to the problem, which differed in how each defined randomness, has led to statistically different threshold numbers of shuffles. This paper reports a comprehensive experimental analysis of the card randomization problem for the purposes of determining 1) which of the two theoretical approaches made the more accurate prediction, 2) whether different statistical tests yield different threshold numbers of randomizing shuffles, and 3) whether manual or mechanical shuffling randomizes a deck more effectively for a given number of shuffles. Permutations of 52-card decks, each subjected to sets of 19 successive riffle shuffles executed manually and by an auto-shuffling device were recorded sequentially and analyzed in respect to 1) the theory of runs, 2) rank ordering, 3) serial correlation, 4) theory of rising sequences, and 5) entropy and information theory. Among the outcomes, it was found that: 1) different statistical tests were sensitive to different patterns indicative of residual order;2) as a consequence, the threshold number of randomizing shuffles could vary widely among tests;3) in general, manual shuffling randomized a deck better than mechanical shuffling for a given number of shuffles;and 4) the mean number of rising sequences as a function of number of manual shuffles matched very closely the theoretical predictions based on the Gilbert-Shannon-Reed (GSR) model of riffle shuffles, whereas mechanical shuffling resulted in significantly fewer rising sequences than predicted. 展开更多
关键词 RANDOMIZATION of cards Number of RIFFLE Shuffles Rising Sequences GSR Model Entropy and Information
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