A cDNA clone encoding a putative EBF-like protein （DCEBF1）was obtained from total RNA isolated from senescing carnation （Dianthus caryophyllus L.） petals using reverse transcription PCR and rapid-amplification of ...A cDNA clone encoding a putative EBF-like protein （DCEBF1）was obtained from total RNA isolated from senescing carnation （Dianthus caryophyllus L.） petals using reverse transcription PCR and rapid-amplification of cDNA ends techniques. The cDNA contained an open reading frame of l 878 bp corresponding to 625 amino acids. Results of Northern blot indicated DCEBFI expression was enhanced by endogenous and exogenous ethylene, and was inhibited by STS in petals and ovaries. Upon wounding treatment, DCEBF1 showed a quick increase in mRNA accumulation which was positively correlated with the increase in ethylene production. The levels of DCEBF1 mRNA increased in both petals and ovaries by sucrose treatment compared with the control.展开更多
Using sucrose, citric acid and vitamin C as the basic liquid, different concentrations of 6-BA and B9 preservatives were added, and the carnation variety ＇ Master＇ was treated. The results showed that the formula of...Using sucrose, citric acid and vitamin C as the basic liquid, different concentrations of 6-BA and B9 preservatives were added, and the carnation variety ＇ Master＇ was treated. The results showed that the formula of 3% sucrose ＋ 50 mg/L citric acid ＋ 600 mg/L vitamin C ＋ 20 mg/L 6-BA could effectively maintain the water balance of the cut flower, prolong the vase life of the cut flower to 16.4 d, and maximize the flower diameter and the crown height.展开更多
In order to distinguish eight carnation cultivars, 40 samples were analyzed by Fourier transform infrared （FI＇IR） spectroscopy combined with principal component analysis （PCA） and hierarchical cluster analysis （...In order to distinguish eight carnation cultivars, 40 samples were analyzed by Fourier transform infrared （FI＇IR） spectroscopy combined with principal component analysis （PCA） and hierarchical cluster analysis （HCA）. According to the results, infrared spectra of eight carnation cuhivars were similar, but signifi- cant differences were observed in wave numbers and absorption peak intensities in the range of 1 800 -700cm-1. The second order derivative spectra in the range of 1 800 -700 cm -l were selected to perform principal component analysis （PCA） and hierarchical cluster analysis （HCA）. The cumulative contribution rate of the first three principal components reached 96.2%. The classification accuracy rate of PCA and HCA was 95% and 100%, respectively. The results demonstrated that Fourier transform infrared （FTIR） spectroscopy combined with principal component analysis （PCA） and hierarchical cluster analysis （HCA） could be used for identification of different carnation cultivars.展开更多
Production of cut flowers is very prevalent in Iran. Carnation is most famous for its use as a cut flower. This study was carried out in the research greenhouse of Islamic Azad University, Isfahan (Khorasgan) Branch, ...Production of cut flowers is very prevalent in Iran. Carnation is most famous for its use as a cut flower. This study was carried out in the research greenhouse of Islamic Azad University, Isfahan (Khorasgan) Branch, to explore the effect of different growing media on some vegetative and reproductive traits of carnation. The experimental design was randomized complete, using 50% perlite and different growing media including: mineral soil and coco peat in 6 proportions (50% coco peat, 10% soil + 40% coco peat, 20% soil + 30% coco peat, 30% soil + 20% coco peat, 40% soil + 10% coco peat, 50% soil). During growth period, irrigation rate, humidity and temperature were similar. The growth characteristics of carnation were determined at the end of growth period. The results indicated that although there were no significant differences between studied growing substrates in chlorophyll a, b. Statistical analysis showed fresh weight of flower was far greater in coco peat 10% + soil 40% and soil 50% compared to other growing media. Fresh and dry weight of root and shoot in soil 50% were higher than other growing media. The results showed that highest concentration of Fe and Zn was observed in coco peat 40% + soil 10%. The concentration of Mn in coco peat 10% + soil 40% was higher than other growing media. The results showed that the highest Ca concentration was observed in soil 50% and the lowest concentration of this element was obtained in coco peat 50%. According to the obtained results, mineral soil can be used as a suitable alternative to cocopeat for cultivation and growth of carnation in greenhouse.展开更多
基金supported by the National Natural Science Foundation of China (30800758 and 30972410)SRF for ROCS,SEM,Fok Ying Tung Education Foundation (104031),Chinathe Plant Biology National Experimental Teaching Demonstration Center of South China Agricultural University,China
文摘A cDNA clone encoding a putative EBF-like protein （DCEBF1）was obtained from total RNA isolated from senescing carnation （Dianthus caryophyllus L.） petals using reverse transcription PCR and rapid-amplification of cDNA ends techniques. The cDNA contained an open reading frame of l 878 bp corresponding to 625 amino acids. Results of Northern blot indicated DCEBFI expression was enhanced by endogenous and exogenous ethylene, and was inhibited by STS in petals and ovaries. Upon wounding treatment, DCEBF1 showed a quick increase in mRNA accumulation which was positively correlated with the increase in ethylene production. The levels of DCEBF1 mRNA increased in both petals and ovaries by sucrose treatment compared with the control.
文摘Using sucrose, citric acid and vitamin C as the basic liquid, different concentrations of 6-BA and B9 preservatives were added, and the carnation variety ＇ Master＇ was treated. The results showed that the formula of 3% sucrose ＋ 50 mg/L citric acid ＋ 600 mg/L vitamin C ＋ 20 mg/L 6-BA could effectively maintain the water balance of the cut flower, prolong the vase life of the cut flower to 16.4 d, and maximize the flower diameter and the crown height.
基金Supported by National Natural Science Foundation of China"Vibration Spectrum-based Diagnosis of Biological Diseases in Broad Bean"(30960179)Science and Technology Innovation Program for Universities in Yunnan Province
文摘In order to distinguish eight carnation cultivars, 40 samples were analyzed by Fourier transform infrared （FI＇IR） spectroscopy combined with principal component analysis （PCA） and hierarchical cluster analysis （HCA）. According to the results, infrared spectra of eight carnation cuhivars were similar, but signifi- cant differences were observed in wave numbers and absorption peak intensities in the range of 1 800 -700cm-1. The second order derivative spectra in the range of 1 800 -700 cm -l were selected to perform principal component analysis （PCA） and hierarchical cluster analysis （HCA）. The cumulative contribution rate of the first three principal components reached 96.2%. The classification accuracy rate of PCA and HCA was 95% and 100%, respectively. The results demonstrated that Fourier transform infrared （FTIR） spectroscopy combined with principal component analysis （PCA） and hierarchical cluster analysis （HCA） could be used for identification of different carnation cultivars.
文摘Production of cut flowers is very prevalent in Iran. Carnation is most famous for its use as a cut flower. This study was carried out in the research greenhouse of Islamic Azad University, Isfahan (Khorasgan) Branch, to explore the effect of different growing media on some vegetative and reproductive traits of carnation. The experimental design was randomized complete, using 50% perlite and different growing media including: mineral soil and coco peat in 6 proportions (50% coco peat, 10% soil + 40% coco peat, 20% soil + 30% coco peat, 30% soil + 20% coco peat, 40% soil + 10% coco peat, 50% soil). During growth period, irrigation rate, humidity and temperature were similar. The growth characteristics of carnation were determined at the end of growth period. The results indicated that although there were no significant differences between studied growing substrates in chlorophyll a, b. Statistical analysis showed fresh weight of flower was far greater in coco peat 10% + soil 40% and soil 50% compared to other growing media. Fresh and dry weight of root and shoot in soil 50% were higher than other growing media. The results showed that highest concentration of Fe and Zn was observed in coco peat 40% + soil 10%. The concentration of Mn in coco peat 10% + soil 40% was higher than other growing media. The results showed that the highest Ca concentration was observed in soil 50% and the lowest concentration of this element was obtained in coco peat 50%. According to the obtained results, mineral soil can be used as a suitable alternative to cocopeat for cultivation and growth of carnation in greenhouse.
