Comparative Study on Muscle Fiber Characteristics and Muscle Nutritional Quality between Two Groups of Yellow River Carps

The aim was to protect the Henan Yellow River carp germplasm resources and provide a scientific basis for the meat quality improvement of Yellow River carps. With artificially farmed and wild Henan Yellow River carps as the research objects, comparative study on muscle fiber diameter and density, routine nutritional composition, calcium and phosphorus contents and amino acids composition was conducted between the two groups of carps. The results showed that the moisture content was significantly higher （P 〈0.05）, the crude fat and crude protein contents were significantly lower （P〈0.05）, the essential amino acids and total amino acids contents were lower （P〉0.05）, the phosphorus content was higher （P 〉0.05）, and the calcium content was lower （P〉0.05） in the wild group compared with those in the farming group. The analysis of muscle fiber characteristics showed that there were significant differences in the average muscle fiber diameter （P〈0.05） and muscle fiber density （P〈0.01） between the two groups of Henan Yellow River carps.

移动自组网的CARPs协议分析

移动自组网备受关注的同时也暴露了其在某些方面的局限性,尤其是能量、带宽问题,因此有必要设计最佳路由协议来减少能量消耗、缩短路由、限制泛播,介绍了2种自适应的、无链接的路由协议及其子系统。当采用定向天线时,该协议能减少在分组传送过程中涉及的节点数量,因此减少了能量消耗,充分利用带宽。

Effects of Nano-sustained Release Sodium Butyrate on Growth and Intestinal Cell Proliferation of Grass Carps

Taking grass carps with the initial weight of about 20g as the research object,the basic feeds of grass carps were added with0.0%,0.1%,0.3%,0.6%,0.8%,and 1% of nano-sustained release sodium butyrate to prepare 6 types of experimental feeds with equal nitrogen and energy.The effects of different concentrations of nano-sustained release sodium butyrate were surveyed on growth and intestinal cell proliferation of grass carps.The experiment was carried out in cages with 50 carps per cage,and each treatment was repeated 3 times for60 days.Experimental results indicated that the addition of nano-sustained release sodium butyrate significantly promoted the growth of grass carps and significantly increased the ratio of intestinal villus to crypt depth.When the addition of nano-sustained release sodium butyrate was0.6%,the weight increase rate,specific growth rate,fullness and intestinal villus height of grass carps were the highest,which was significantly higher than that of the control group(P < 0.05).The study results indicated that addition of appropriate amount of nano-sustained release sodium butyrate can promote the growth of grass carps through increasing the intestinal villus height,and the suitable addition dosage was0.6%.

Prevalence of Ectoparasitic Infestation in Indian Major Carps During Winter at Different Blocks of South 24-Parganas District, West Bengal, India

The study was carried out to find the prevalence of ectoparasites in carp species specifically Indian Major Carps(IMC)during the post monsoon season(November’16 to March’17).Four groups of ectoparasites viz.myxozoan,ciliophoran,monogenean and crustacean were recorded from a total 500 number of carp species like,Labeo rohita,Catla catla,Cirrhinus mrigala and Labeo calbasu collected from different ponds of selected blocks of South 24-Parganas district of West Bengal.The highest prevalence(64.8%)of infestation had been recorded by Myxozoans and the lowest was by Monogeneans(4.8%).The highest and lowest ectoparasitic prevalence in carp was observed in L.rohita(32.9%)and C.catla(27.3%).Beside these,lower temperature(Average 19.3℃),low pH(Average 6.9)and marginal level of dissolved oxygen(Average 6.0ppm)were also created an unfavorable condition for parasitic infestation during this season.At the end of this experiment it was concluded that disease occurrences due to ectoparasites was high in winter with some key factors like temperature,pH and dissolved oxygen(DO).

The Potential for Homestead Pond Polyculture of Tilapia and Carps in Coastal Bangladesh

A fishers’women-led Participatory Action Research(PAR)was conducted in 30 homestead ponds to assess the potential for polyculture of Tilapia(Oreochromis niloticus)and major carps Rohu(Labeo rohita)and Catla(Catla catla)in two coastal fishing villages of Bangladesh.Three treatments,namely T1(Tilapia 200 fish per decimal;1 decimal=40 m^2),T2(Tilapia 200+Rohu 32+Catla 8 fish per decimal)and T3(Tilapia 200+Rohu 8+Catla 32 fish per decimal),each with 5 replicates,were tried in Hossainpur and Anipara villages.Formulated commercial Mega-feed was applied to the ponds twice daily at an initial rate of 10%body weight(bw)/day of Tilapia down to 4%bw/day throughout the culture period.The water quality parameters of ponds including transparency,salinity and dissolved oxygen significantly varied among treatments except temperature and pH and remained within optimum range for carp polyculture except salinity.The survival rate,harvesting weight and yield of Tilapia were significantly highest in T1(85.63±05%,258.59±18.76 g&11073±805 kg/ha,respectively)in Anipara and lowest in T3(75.63±0.37%,136.97±10.63 g&5180±406 kg/ha,respectively)in Hossainpur.The gross fish production was the significantly highest in T1(11354±806 kg/ha)of Anipara and lowest in T1(6325±227 kg/ha)of Hossainpur.Statistically,the highest net return(866,627±84874 BDT/ha)was found in T1 of Anipara and lowest in T3(279,389±46104 BDT/ha)of Hossainpur with a significantly higher benefit-cost ratio(BCR)obtained in T1(3.26±0.20)for Tilapia and lower in T3(1.58±0.10)for polyculture Tilapia and carp of Hossainpur.Therefore,it may be concluded that Tilapia production in small homestead coastal ponds has a higher potential than its mix with carps in polyculture for generating food and supplemental income opportunity for coastal fishers’women in Bangladesh.

Prevalence of Argulus sp. in Indian Major Carps from Bhangore Block of South 24 Parganas District, West Bengal, India

The study was carried out to find the prevalence and severity of Argulus sp.in Indian Major Carps(IMCs)collected from Bhangore block of South 24 Parganas district of West Bengal during November 2018 to August 2019.January(2019)recorded the highest parasitic prevalence(PFI,83.33%)and the month of August(2019)recorded the lowest parasitic prevalence(PFI,9%).The identities of selected parasites were further confirmed by molecular identification through 18S rDNA analysis.The study revealed that Argulus sp.infestations had great economic implications especially in the winter months and is one of the most prevalent problems in fresh water aquaculture systems.

Impact of the Three Gorges Dam on reproduction of four major Chinese carps species in the middle reaches of the Changjiang River

Silver carp(Hypophthalmichthys molitrix),bighead carp(Aristichthys nobilis),grass carp(Ctenopharyngodon idella),and black carp(Mylopharyngodon piceus) are the four major Chinese carps and are commercially important aquaculture species in China.Reproduction of these carp has declined since the construction of the Three Gorges Dam(TGD) due to an altered water flow and thermal regime in the Changjiang(Yangtze) River.However,details of the changes in reproduction of the four species are not well understood.To assess the impact of the TGD on reproduction of the four carp,we investigated their eggs and larvae at Yidu City,which is 80 km below the TGD,during 2005-2012.We examined differences in larval abundance of the four species in the Jianli section(350 km downstream of the TGD) before(1997-2002)and after(2003-2012) construction of the TGD.Based on these observations,the first spawning date of the four species was delayed a mean of about 25 days after the dam was constructed.Mean egg abundance in the Yidu section of the river was 249 million and mean larval abundance was 464 million,which were significant decreases since the 1980 s.Moreover,larval abundance in the Jianli section after the dam was constructed was significantly lower than that before construction(ANCOVA,P<0.05).The observed larval abundance accounted for only 24.66%of the predicted value in 2003 when the dam was first inundated.The present spawning grounds between the TGD and Yidu section of the river are very similar to those described in the 1980 s,and some spawning grounds exist upstream of the TGD.Large free-flowing stretches upstream of the TGD and the creation of artificially flooded downstream reaches are needed to stimulate spawning and effectively conserve these four major Chinese carps species.

Effects of Benzo[a]pyrene on SOD and CAT in Kidneys from Carps

With carps as the object of study, the effects of benzo[a]pyrene concentration on SOD and CAT in carp kidneys were studied. There were three treatment groups and one control group. The exposure method was the static method. The superoxide dismutase （SOD） and catalase （CAT） activity was determined 30 d later. The results showed that after exposing to different concentrations of benzo[a]pyrene （0, 0.1, 0.5 and 1.0 μg /L） for 30 d, the SOD activity was higher in the low concentration group （0.1 μg/L） than in the control group; the 0.5 μg/L benzo[a]pyrene group had no significant difference from the control group; and under the concentration of 1.0 μg/L, the SOD activity decreased remarkably. The CAT activity was higher in the 0.1 μg/L group than in the control group, and decreased significantly in the 1.0 μg/L benzo[a]pyrene group compared with the control group. The results showed that benzo[a]pyrene had certain effects on the antioxidant enzymes in carp kidneys. This study provides a theoretical basis for water environmental pollution.

Enteric Red Mouth disease and its causative bacterium, Yersinia ruckeri, in Indian Major Carps from culture ponds in Andhra Pradesh, India

During the present investigation,studies were carried out to investigate the outbreak of Redmouth disease in culture ponds situated at Krishna,West Godavari and East Godavari districts of Andhra Pradesh.Enteric Red Mouth disease or Yersiniosis is a serious infectious bacterial disease,sometimes responsible for causing severe economic loss to fish farming industry.The disease is clinically characterized by symptoms like bleeding at the base of fins,inside and around mouth,bilateral exophthalmia with or without haemorrhage,and per-ocular and peri-oral haemorrhages.In the present study,live or moribund fish showing lesions similar to enteric redmouth disease were collected and the causative agent was isolated by culture techniques.Bacterial identification was carried out by conventional biochemical methods and molecular polymerase chain reaction.DNA extracted from isolated pathogen was subjected to PCR amplification with a pair of Y.ruckeri specific primers.The PCR product was run on 1.5%agarose gel electrophoresis and positive bands with a molecular size of approximately 500bp were detected.It is similar to that given for PCR products with primers specific for Y.ruckeri.

Endosymbiotic pathogen-inhibitory gut bacteria in three Indian Major Carps under polyculture system: A step toward making a probiotics consortium

The gastrointestinal(GI)microbiome in fish plays significant roles in health and disease resistance.This investigation was accomplished to enumerate,characterize and identify the potential probiotic bacteria from three Indian Major Carps(IMCs),viz.,rohu(Labeo rohita),catla(Catla catla)and mrigal(Cirrhinus mrigala)using culture dependent methods.Altogether,105 pathogen-inhibitory bacteria(out of 1216 isolates)were detected from three IMCs by double layer assay.16S rRNA partial gene sequence analyses and BLAST search in the NCBI GenBank unveiled that 94.29%of the pathogen inhibitory bacteria were bacilli(99 strains)and Bacillus licheniformis by far the most common(28%).The primarily selected 27 pathogen-inhibitory strains(cumulative inhibition score≥13)produced extracellular enzymes,while 15 of them produced all the six exo-enzymes studied(amylase,protease,lipase,cellulase,phytase and xylanase).Gut stability of the strains became apparent by their ability to grow in fish mucus and tolerance to diluted bile-juice.Finally,14 strains were noticed asγ-hemolytic and susceptible to the commonly used antibiotics.Further,intra-peritoneal injection withγ-hemolytic strains did not induce any pathological signs or mortalities in fish,and thus were considered as safe.These 14γ-hemolytic isolates were represented by the genus Bacillus(13)and Stenotrophomonas(1),which might form probiotic consortia for prospective use in carp culture.

Enzyme-linked immunosorbent assay of changes in serum levels of growth hormone(cGH)in common carps(Cyprinus carpio)

The aim of the present study was to purify the common native carp growth hormone (ncGH), produce monoclonal antibodies (mAbs) to common native carp growth hormone (ncGH), and further enhance the sensitivity of enzyme-linked immunosorbent assays (ELISA) for ncGH. Additionally, we investigated changes in serum ncGH levels in carps raised in different environmental conditions. The recombinant grass carp (Ctenopharyngodon idella) growth hormone was purified and used as antigen to immunize the rabbit. The natural ncGH was isolated from the pituitaries of common carp. SDS-PAGE and Western blot utilizing the polyclonal anti-rgcGH antibody confirmed the purification of ncGH from pituitaries. Purified ncGH was then used as an immunogen in the B lymphocyte hybridoma technique. A total of 14 hybridoma cell lines (FMU-cGH 1-14) were established that were able to stably secrete mAbs against ncGH. Among them, eight clones (FMU-cGH1-6, 12 and 13) were successfully used for Western blot while nine clones (FMU-cGH 1-7, 9 and 10) were used in fluorescent staining and immunohistochemistry. Epitope mapping by competitive ELISA demonstrated that these mAbs recognized five different epitopes. A sensitive sandwich ELISA for detection of ncGH was developed using FMU-cGH12 as the coating mAb and FMU-cGH6 as the enzyme labeled mAb. This detection system was found to be highly stable and sensitive, with detection levels of 70 pg/mL. Additionally, we found that serum ncGH levels in restricted food group and in the net cage group increased 6.9-and 5.8-fold, respectively, when compared to controls, demonstrating differences in the GH stress response in common carp under different living conditions.

Unveiling the emerging role of curcumin to alleviate ochratoxin A-induced muscle toxicity in grass carp(Ctenopharyngodon idella):in vitro and in vivo studies

Background Ochratoxin A(OTA),a globally abundant and extremely hazardous pollutant,is a significant source of contamination in aquafeeds and is responsible for severe food pollution.The developmental toxicity of OTA and the potential relieving strategy of natural products remain unclear.This study screened the substance curcumin(Cur),which had the best effect in alleviating OTA inhibition of myoblast proliferation,from 96 natural products and investigated its effect and mechanism in reducing OTA myotoxicity in vivo and in vitro.Methods A total of 720 healthy juvenile grass carp,with an initial average body weight of 11.06±0.05 g,were randomly assigned into 4 groups:the control group(without OTA and Cur),1.2 mg/kg OTA group,400 mg/kg Cur group,and 1.2 mg/kg OTA+400 mg/kg Cur group.Each treatment consisted of 3 replicates(180 fish)for 60 d.Results Firstly,we cultured,purified,and identified myoblasts using the tissue block culture method.Through preliminary screening and re-screening of 96 substances,we examined cell proliferation-related indicators such as cell viability and ultimately found that Cur had the best effect.Secondly,Cur could alleviate OTA-inhibited myoblast differentiation and myofibrillar development-related proteins(Myo G and MYHC)in vivo and in vitro and improve the growth performance of grass carp.Then,Cur could also promote the expression of OTA-inhibited protein synthesis-related proteins(S6K1 and TOR),which was related to the activation of the AKT/TOR signaling pathway.Finally,Cur could downregulate the expression of OTA-enhanced protein degradation-related genes(murf1,foxo3a,and ub),which was related to the inhibition of the Fox O3a signaling pathway.Conclusions In summary,our data demonstrated the effectiveness of Cur in alleviating OTA myotoxicity in vivo and in vitro.This study confirms the rapidity,feasibility,and effectiveness of establishing a natural product screening method targeting myoblasts to alleviate fungal toxin toxicity.

Anti-infective immune functions of type Ⅳ interferon in grass carp(Ctenopharyngodon idella):A novel antibacterial and antiviral interferon in lower vertebrates

Type Ⅳ interferon(IFN-υ)is a recently discovered cytokine crucial for host defense against viral infections.However,the role and mechanisms of IFN-υin bacterial infections remain unexplored.This study investigated the antibacterial and antiviral functions and mechanisms of grass carp(Ctenopharyngodon idella)IFN-υ(CiIFN-υ)both in vivo and in vitro.The CiIFN-υgene was first identified and characterized in grass carp.Subsequently,the immune expression of CiIFN-υsignificantly increased following bacterial challenge,indicating its response to bacterial infections.The eukaryotic recombinant expression plasmid of CiIFN-υwas then constructed and transfected into fathead minnow(FHM)cells.Supernatants were collected and incubated with four bacterial strains,followed by plate spreading and colony counting.Results indicated that CiIFN-υexhibited more potent antibacterial activity against gram-negative bacteria compared to gram-positive bacteria and aggregated gram-negative bacteria but not gram-positive bacteria.In vivo experiments further confirmed the antibacterial function,showing high survival rates,low tissue edema and damage,reduced tissue bacterial load,and elevated proinflammatory response at the early stages of bacterial infection.In addition,the antiviral function of CiIFN-υwas confirmed through in vitro and in vivo experiments,including crystal violet staining,survival rates,tissue viral burden,and reverse transcription-quantitative real-time polymerase chain reaction(RT-qPCR).This study highlights the antibacterial function and preliminary mechanism of IFN-υ,demonstrating that IFN-υpossesses dual functions against bacterial and viral infections.

Electrophoretic Analysis of Isozymes and Discussion about Species Differentiation in Three Species of Genus Gymnocypris

By using the method of electrophoresis,three isozymes (lactate dehydrogenase,malate dehydrogenase and esterase) of three species of genus Gymnocypris were described and analyzed from North Tibet in this paper. The results showed that all three isozymes presented interspecific difference and distinct differentiation among individuals in the same population,and there was no electrophorectic difference between males and females. Analysis of relationships among three naked carps indicated a high degree of similarity between G. selincuoensis and G. cuoensis ,whereas low degree between G. selincuoensis and G. namensis . Furthermore,three isozymes presented expression of null alleles,and the duplicate genes of LDH A 2,LDH B 2,s MDH A 2 and m MDH B 2 also expressed in some individuals. Compared to other tetraploid fishes,three naked carps retained more functional duplicate genes and null alleles. This suggests fishes of genus Gymnocypris are at the early stage of evolution after polyploidization than that of fishes of Catostomidae,it directly related to the later time of schizothoracine fishes originate as well as severe environment.

Research Progress on Nutritional Requirements of Carp

Carp is a temperate freshwater fish native to Asia,distributed in all regions of the world except Australia and South America.With the improvement of comprehensive and healthy breeding technology of carp,the unit yield has been greatly increased mainly due to the exten-sive use of compound feed.In this study,the nutritional requirements of carp were summarized from the aspects of protein,amino acids,fat,carbohydrate,calcium and phosphorus,vitamins and taurine.This study provides a certain theoretical reference for scientific formula of carp feed.

异育银鲫“中科3号”与丰产鲫形态特征及生长的比较研究

池养条件下,比较研究了异育银鲫(Carassius auratus gibelio)"中科3号"和丰产鲫(Carassius auratus Var.Pengzenensis)的形态特征与生长。结果显示:体长/体高、体长/头长、体长/尾柄长、尾柄长/尾柄高等可量性状参数比值差异显著,可作为两者形态区别主要参数;其中异育银鲫"中科3号"的体长为体高的2.55倍,为头长的4.27倍,为尾柄长的8.56倍,尾柄长为尾柄高的0.76倍;丰产鲫的体长为体高的2.74倍,为头长的3.66倍,为尾柄长的5.87倍,尾柄长为尾柄高的1.14倍;异育银鲫"中科3号"体略高,头略短,尾柄略短略高,丰产鲫体型较修长。两种鲫鱼体长体重增长率差异显著,养殖前3个月,丰产鲫体长体重增长率高过异育银鲫"中科3号",养殖后4个月,异育银鲫"中科3号"的体长体重增长率高过丰产鲫;两种鲫鱼肥满度指数(K)在不同养殖阶段有一定变化(2.71～3.12),但差异不显著;综合评定异育银鲫"中科3号"生长性能优于丰产鲫,适宜华南地区推广养殖。

Comparative Microsatellite Analysis of Grass Carp Genomes of Two Gynogenetic Groups and the Xiangjiang River Group

The genomes of three groups of grass carp, namely the Xiangjiang River grass carp group （Xiangjiang group）, a one-generation artificially induced meio-gynogenetic grass carp group （meio-gynogenetic-1 group）, and a two-generation artificially induced meio-gynogenetic grass carp group （meio-gynogenetic-2 group）, were comparatively analyzed with microsatellite markers. Genetic polymorphism had been observed in the Xiangjiang group and most of the examined loci had more than two alleles. But the degree of genetic diversity was not very high. Although all the examined genetic loci in the analyzed individuals were in homozygous state, the genotypes of different individuals of the group were not identical in the meio-gynogenetic-1 group. In the meio-gynogenetic-2 group, not only the examined genetic loci of each individual were homozygous but also the genotypes of all the analyzed individuals of the group were the same. These results suggested that the examined meio-gynogenetic-2 group is a homozygous group and homozygous clone could be produced by continuous artificial induction of gynogenesis for two generations. It was found that the polymorphism existed not only at the allele level but also at the locus level; many alleles of the microsatellite loci and some of the microsatellite loci had been lost during the process of artificial gynogenesis. Therefore, both protection of the diversity of natural grass carp resource and selection of homozygous traits with desired economic genotypes are very important aspects for grass carp breeding.

Microsatellite DNA Marker Analysis of Genetic Diversity in Wild Common Carp (Cyprinus carpio L.) Populations

Thirty microsatellite loci were used for analyzing six wild populations of common carp （Cyprinus carpio L.）. Observed （Ho） and expected （He） heterozygosity values, polymorphic information content （PIC）, and number of effective alleles （Ae） were all detected. Genetic similarity index and genetic distance were computed based on the allele frequency. The Hardy-Weinberg Equilibrium was checked according to the test of χ^2. Genetic differentiation and hierarchical partition of genetic diversity were evaluated by FST and Nm. A clustering dendrograrn was made based on the results of UPGMA methods using the PHYLIP software package （version 3.63）. There were totally 8,136 fragments ranging from 125 bp to 414 bp in length. Three to thirteen alleles were amplified in 30 loci and 210 alleles in all six populations. The average number of alleles in each locus was seven. The result showed that 1） the level of genetic variability was moderate in the six populations. Polymorphic information contents of the six wild common carp populations were 0.44, 0.52, 0.53, 0.57, 0.63, and 0.64 respectively. Effective alleles were from 1.04 to 4.72, the average numbers in each population were 2.19, 2.60, 2.42, 2.43, 2.45, and 2.33. The average expected heterozygosity values were 0.50, 0.59, 0.56, 0.56, 0.57, and 0.54 respectively; 2） the highest genetic similarity index that came from the populations of BR and ZL was 0.8511 and the lowest index was 0.6688, and it came from the populations of BR and HN. There was a correlation between the clustering result and the geographical distribution.

一种求解多车型CARP问题的高效进化算法

对传统遗传算法的染色体编码机制和种群结构进行了改进,并借鉴单亲遗传算法和Memetic Algorithm(MA)算法的优秀思想,设计了一种解决CARP(Capacitated ArcRouting Problem)问题的高效算法HEGA。新算法不但有效解决了使用现有算法无力解决的多车型CARP问题,并且应用于一般的单车型CARP问题在求解效率和求解精度上也比现有MA算法效果更好。结合洒水车路径优化问题,通过一组真实的数据集合对文中算法在该问题上的求解能力做出评测。