Background: Catheter-related infections (CRI), thrombosis, and stenosis are among the most frequent complications associated with catheters which are inserted in vessels as vascular access. These problems are usually ...Background: Catheter-related infections (CRI), thrombosis, and stenosis are among the most frequent complications associated with catheters which are inserted in vessels as vascular access. These problems are usually related to the handling of the staff, the catheter materials, and the surface properties of the catheter. To mitigate such complications surface treatment process of the outer surface, such as ion beam assisted deposition is investigated in a retrospective study from 1992 to 2007, to prove if the surface treatment of the catheters is a sufficient solution. Methods: This study (1992-2007) evaluated silver coated and non-coated implanted large-bore catheters used for extracorporeal detoxification. In 159 patients, 54 patients received a silver coated catheter (Spi-Argent, Spire Corporation, Bedford, MA, USA) and 105 patients, an untreated catheter served as controls. The catheters were inserted into the internal jugular or subclavian veins. After removal, the catheters were cultured for bacterial colonization using standard microbiologic assays. They were also examined using a scanning electron microscope (SEM). Results: The silver coated catheters showed a tendency towards longer in situ time. The microbiologic examinations of the catheter tips were in both catheter types high positive, but not significant. Conclusion: The silver coated catheters showed no significant reduction in infection rate by evaluation of all collected data in this retrospective study. There was no association between both catheters in significantly reducing patient discomfort. Other surface treatments which include the outer and inner surface are necessary. New developed catheter materials such as the microdomain structured inner and outer surface, as an example, are considered more biocompatible because they mimic the structure of natural biological surface.展开更多
Bacterial infection and tissue damage caused by friction are two major threats to patients’health in medical catheter implantation.Hydrogels with antibacterial and lubrication effects are competitive candidates for c...Bacterial infection and tissue damage caused by friction are two major threats to patients’health in medical catheter implantation.Hydrogels with antibacterial and lubrication effects are competitive candidates for catheter coating materials.Photothermal therapy(PTT)is a highly efficient bactericidal method.Here,a composite hydrogel containing MXene nanosheets and hydrophilic 3-sulfopropyl methacrylate potassium salt(SPMK)is reported,which is synthesized through the one-pot method and heat-initiated polymerization.The hydrogel shows excellent antibacterial performance against Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)in 3 min in the air or 20 min in the water environment under near-infrared light(NIR;808 nm)irradiation.The friction coefficient of the hydrogel is about 0.11,which is 48%lower than that without SPMK.The rapid photothermal sterilization is attributed to the outstanding antibacterial ability and thermal effect of photoactivated MXene.The ultra-low friction is the result of the hydration lubrication mechanism.This study provides a potential strategy for the surface coatings of biomedical catheters,which enables rapid sterilization and extremely low interface resistance between catheters and biological tissues.展开更多
Background:A new type of silver alloy hydrogel-coated(SAH)catheter has been shown to prevent bacterial adhesion and colonization by generating a microcurrent,and to block the retrograde infection pathway.However,these...Background:A new type of silver alloy hydrogel-coated(SAH)catheter has been shown to prevent bacterial adhesion and colonization by generating a microcurrent,and to block the retrograde infection pathway.However,these have only been confirmed in ordinary patients.This study aims to evaluate the effectiveness of a SAH catheter for preventing urinary tract infections in critically ill patients.Methods:This was a prospective single-center,single-blind,randomized,controlled study.A total of 132 patients requiring indwelling catheterization in the intensive care unit(ICU)of the First Affiliated Hospital of the University of Science and Technology of China between October 2022 and February 2023 and who met the study inclusion/exclusion criteria were randomly divided into two groups.Patients in the SAH catheter group received a SAH catheter,while patients in the conventional catheter group received a conventional siliconized latex Foley catheter.The main outcome measure was the incidence of catheter-associated urinary tract infections(CAUTIs).Secondary outcome indicators included urine positivity for white blood cells and positive urine cultures on 3 days,7 days,10 days,and 14 days after catheterization,number of viable bacteria in the catheter biofilm on day 14,pathogenic characteristics of positive urine cultures,length of ICU stay,overall hospital stay,ICU mortality,and 28-day mortality.All the data were compared between the two groups.Results:A total of 68 patients in the conventional catheter group and 64 patients in the SAH catheter group were included in the study.On day 7 after catheter placement,the positivity rate for urinary white blood cells was significantly higher in the conventional catheter group than in the SAH catheter group(33.8%vs.15.6%,P=0.016).On day 10,the rates of positive urine cultures(27.9%vs.10.9%,P=0.014)and CAUTIs(22.1%vs.7.8%,P=0.023)were significantly higher in the conventional catheter group than in the SAH catheter group.On day 14,the numbers of viable bacteria isolated from the catheter tip([3.21±1.91]×10^(6) colony-forming units[cfu]/mL vs.[7.44±2.22]×10^(4) cfu/mL,P<0.001),balloon segment([7.30±1.99]×10^(7) cfu/mL vs.[3.48±2.38]×10^(5) cfu/mL,P<0.001),and tail section([6.41±2.07]×10^(5) cfu/mL vs.[8.50±1.46]×10^(3) cfu/mL,P<0.001)were significantly higher in the conventional catheter group than in the SAH catheter group.The most common bacteria in the urine of patients in both groups were Escherichia coli(n=13)and Pseudomonas aeruginosa(n=6),with only one case of Candida in each group.There were no significant differences between the two groups in terms of ICU hospitalization time,total hospitalization time,ICU mortality,and 28-day mortality.Conclusion:SAH catheters can effectively inhibit the formation of catheter-related bacterial biofilms in critically ill patients and reduce the incidence of CAUTIs,compared with conventional siliconized latex Foley catheters;however,regular replacement of the catheter is still necessary.展开更多
Catheter-associated urinary tract infections （CAUTIs） are among the most common bacterial infections associated with medical devices. In the current study, the synthesis, coating, antibiofilm properties, and biocomp...Catheter-associated urinary tract infections （CAUTIs） are among the most common bacterial infections associated with medical devices. In the current study, the synthesis, coating, antibiofilm properties, and biocompatibility of urinary catheters coated with Zn-doped CuO （Zn0.12Cu0.880） nanoparticles （NPs） were examined. The doped NPs were synthesized and subsequently deposited on the catheter by the sonochemical method. The coated catheters displayed high antibiofilm activity and promising biocompatibility, as indicated by low in vitro cytotoxicity, negligible associated cytokine secretion, and absence of detectable irritation. The biocompatibility and ability of the Zn-doped CuO coating to inhibit biofilm formation were also evaluated in vivo using a rabbit model. Rabbits catheterized with uncoated catheters scored positive for CAUTI by day 4 of the experiment. In contrast, rabbits catheterized with Zn-doped CuO-coated catheters did not exhibit CAUTI until day 7 or remained completely uninfected for the whole duration of the 7-day experiment. Furthermore, the in vivo biocompatibility assays and examinations supported the biosafety of Zn-doped CuO-coated catheters. Taken together, these data highlight the potential of Zn-doped CuO nanocomposite as effective antibiofilm compound.展开更多
目的:体外观察左氧氟沙星(LFX)涂敷导管对铜绿假单胞菌黏附、定植能力的影响,探讨LFX涂敷导管在铜绿假单胞菌导管相关感染中的应用价值。方法:制备LFX涂敷导管,测定LFX涂敷导管的体外药物累积释放度。实验分为LFX涂敷导管组和聚氯乙烯(P...目的:体外观察左氧氟沙星(LFX)涂敷导管对铜绿假单胞菌黏附、定植能力的影响,探讨LFX涂敷导管在铜绿假单胞菌导管相关感染中的应用价值。方法:制备LFX涂敷导管,测定LFX涂敷导管的体外药物累积释放度。实验分为LFX涂敷导管组和聚氯乙烯(PVC)导管组,2组导管分别浸没于5 mL 50%LB培养液中(含铜绿假单胞菌108 CFU/mL),37℃孵育6h、12h、24h、48h,在各时间点,分别于导管表面和导管培养液进行细菌计数。结果:LFX涂敷导管能够在2h内实现药物的快速释放,2h后LFX涂敷导管药物释放很少,甚至没有释放。LFX对实验菌PAO1的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)分别为0.5mg/L、1.0mg/L;6h、12h、24h孵育时间点,LFX1∶3、LFX1∶6、LFX1∶9涂敷导管表面定植、黏附细菌培养均为阴性;且LFX1∶3、LFX1∶6、LFX1∶9涂敷导管在不同孵育时间点培养液的细菌数较PVC导管培养液的细菌数明显减少(P<0.05)。结论:LFX涂敷导管能有效抑制铜绿假单胞菌黏附、定植,提示LFX涂敷导管可作为预防铜绿假单胞菌导管相关感染的有效策略。展开更多
文摘Background: Catheter-related infections (CRI), thrombosis, and stenosis are among the most frequent complications associated with catheters which are inserted in vessels as vascular access. These problems are usually related to the handling of the staff, the catheter materials, and the surface properties of the catheter. To mitigate such complications surface treatment process of the outer surface, such as ion beam assisted deposition is investigated in a retrospective study from 1992 to 2007, to prove if the surface treatment of the catheters is a sufficient solution. Methods: This study (1992-2007) evaluated silver coated and non-coated implanted large-bore catheters used for extracorporeal detoxification. In 159 patients, 54 patients received a silver coated catheter (Spi-Argent, Spire Corporation, Bedford, MA, USA) and 105 patients, an untreated catheter served as controls. The catheters were inserted into the internal jugular or subclavian veins. After removal, the catheters were cultured for bacterial colonization using standard microbiologic assays. They were also examined using a scanning electron microscope (SEM). Results: The silver coated catheters showed a tendency towards longer in situ time. The microbiologic examinations of the catheter tips were in both catheter types high positive, but not significant. Conclusion: The silver coated catheters showed no significant reduction in infection rate by evaluation of all collected data in this retrospective study. There was no association between both catheters in significantly reducing patient discomfort. Other surface treatments which include the outer and inner surface are necessary. New developed catheter materials such as the microdomain structured inner and outer surface, as an example, are considered more biocompatible because they mimic the structure of natural biological surface.
基金support from the National Natural Science Foundation of China(No.52175187)the Fundamental Research Funds for the Central Universities(No.3102019JC001)the Research Fund of the State Key Laboratory of Solidification Processing(NPU),China(No.2023-TS-06).
文摘Bacterial infection and tissue damage caused by friction are two major threats to patients’health in medical catheter implantation.Hydrogels with antibacterial and lubrication effects are competitive candidates for catheter coating materials.Photothermal therapy(PTT)is a highly efficient bactericidal method.Here,a composite hydrogel containing MXene nanosheets and hydrophilic 3-sulfopropyl methacrylate potassium salt(SPMK)is reported,which is synthesized through the one-pot method and heat-initiated polymerization.The hydrogel shows excellent antibacterial performance against Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)in 3 min in the air or 20 min in the water environment under near-infrared light(NIR;808 nm)irradiation.The friction coefficient of the hydrogel is about 0.11,which is 48%lower than that without SPMK.The rapid photothermal sterilization is attributed to the outstanding antibacterial ability and thermal effect of photoactivated MXene.The ultra-low friction is the result of the hydration lubrication mechanism.This study provides a potential strategy for the surface coatings of biomedical catheters,which enables rapid sterilization and extremely low interface resistance between catheters and biological tissues.
基金supported by the Anhui Provincial Key Research and Development Program(grant number:202104j07020043)the Natural Science Research Project of Colleges and Universities in Anhui Province(grant number:2022AH051264).
文摘Background:A new type of silver alloy hydrogel-coated(SAH)catheter has been shown to prevent bacterial adhesion and colonization by generating a microcurrent,and to block the retrograde infection pathway.However,these have only been confirmed in ordinary patients.This study aims to evaluate the effectiveness of a SAH catheter for preventing urinary tract infections in critically ill patients.Methods:This was a prospective single-center,single-blind,randomized,controlled study.A total of 132 patients requiring indwelling catheterization in the intensive care unit(ICU)of the First Affiliated Hospital of the University of Science and Technology of China between October 2022 and February 2023 and who met the study inclusion/exclusion criteria were randomly divided into two groups.Patients in the SAH catheter group received a SAH catheter,while patients in the conventional catheter group received a conventional siliconized latex Foley catheter.The main outcome measure was the incidence of catheter-associated urinary tract infections(CAUTIs).Secondary outcome indicators included urine positivity for white blood cells and positive urine cultures on 3 days,7 days,10 days,and 14 days after catheterization,number of viable bacteria in the catheter biofilm on day 14,pathogenic characteristics of positive urine cultures,length of ICU stay,overall hospital stay,ICU mortality,and 28-day mortality.All the data were compared between the two groups.Results:A total of 68 patients in the conventional catheter group and 64 patients in the SAH catheter group were included in the study.On day 7 after catheter placement,the positivity rate for urinary white blood cells was significantly higher in the conventional catheter group than in the SAH catheter group(33.8%vs.15.6%,P=0.016).On day 10,the rates of positive urine cultures(27.9%vs.10.9%,P=0.014)and CAUTIs(22.1%vs.7.8%,P=0.023)were significantly higher in the conventional catheter group than in the SAH catheter group.On day 14,the numbers of viable bacteria isolated from the catheter tip([3.21±1.91]×10^(6) colony-forming units[cfu]/mL vs.[7.44±2.22]×10^(4) cfu/mL,P<0.001),balloon segment([7.30±1.99]×10^(7) cfu/mL vs.[3.48±2.38]×10^(5) cfu/mL,P<0.001),and tail section([6.41±2.07]×10^(5) cfu/mL vs.[8.50±1.46]×10^(3) cfu/mL,P<0.001)were significantly higher in the conventional catheter group than in the SAH catheter group.The most common bacteria in the urine of patients in both groups were Escherichia coli(n=13)and Pseudomonas aeruginosa(n=6),with only one case of Candida in each group.There were no significant differences between the two groups in terms of ICU hospitalization time,total hospitalization time,ICU mortality,and 28-day mortality.Conclusion:SAH catheters can effectively inhibit the formation of catheter-related bacterial biofilms in critically ill patients and reduce the incidence of CAUTIs,compared with conventional siliconized latex Foley catheters;however,regular replacement of the catheter is still necessary.
文摘Catheter-associated urinary tract infections （CAUTIs） are among the most common bacterial infections associated with medical devices. In the current study, the synthesis, coating, antibiofilm properties, and biocompatibility of urinary catheters coated with Zn-doped CuO （Zn0.12Cu0.880） nanoparticles （NPs） were examined. The doped NPs were synthesized and subsequently deposited on the catheter by the sonochemical method. The coated catheters displayed high antibiofilm activity and promising biocompatibility, as indicated by low in vitro cytotoxicity, negligible associated cytokine secretion, and absence of detectable irritation. The biocompatibility and ability of the Zn-doped CuO coating to inhibit biofilm formation were also evaluated in vivo using a rabbit model. Rabbits catheterized with uncoated catheters scored positive for CAUTI by day 4 of the experiment. In contrast, rabbits catheterized with Zn-doped CuO-coated catheters did not exhibit CAUTI until day 7 or remained completely uninfected for the whole duration of the 7-day experiment. Furthermore, the in vivo biocompatibility assays and examinations supported the biosafety of Zn-doped CuO-coated catheters. Taken together, these data highlight the potential of Zn-doped CuO nanocomposite as effective antibiofilm compound.
文摘目的:体外观察左氧氟沙星(LFX)涂敷导管对铜绿假单胞菌黏附、定植能力的影响,探讨LFX涂敷导管在铜绿假单胞菌导管相关感染中的应用价值。方法:制备LFX涂敷导管,测定LFX涂敷导管的体外药物累积释放度。实验分为LFX涂敷导管组和聚氯乙烯(PVC)导管组,2组导管分别浸没于5 mL 50%LB培养液中(含铜绿假单胞菌108 CFU/mL),37℃孵育6h、12h、24h、48h,在各时间点,分别于导管表面和导管培养液进行细菌计数。结果:LFX涂敷导管能够在2h内实现药物的快速释放,2h后LFX涂敷导管药物释放很少,甚至没有释放。LFX对实验菌PAO1的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)分别为0.5mg/L、1.0mg/L;6h、12h、24h孵育时间点,LFX1∶3、LFX1∶6、LFX1∶9涂敷导管表面定植、黏附细菌培养均为阴性;且LFX1∶3、LFX1∶6、LFX1∶9涂敷导管在不同孵育时间点培养液的细菌数较PVC导管培养液的细菌数明显减少(P<0.05)。结论:LFX涂敷导管能有效抑制铜绿假单胞菌黏附、定植,提示LFX涂敷导管可作为预防铜绿假单胞菌导管相关感染的有效策略。
