Cationic polysaccharides have been receiving more attentions and used as nonviral gene delivery vectors. In this paper, quaternized hydroxyethylcellulose （QHEC） derivatives were studied as gene carriers for their ef...Cationic polysaccharides have been receiving more attentions and used as nonviral gene delivery vectors. In this paper, quaternized hydroxyethylcellulose （QHEC） derivatives were studied as gene carriers for their efficient DNA binding abilities. All QHECs could form stable QHEC/DNA complexes and resist the degradation of DNase I. And the dynamic light scatter （DLS） results showed that all QHEC/DNA complexes could form compact particles. These QHEC/DNA complexes exhibited effective transfeetion abilities in comparison to the naked DNA. The cytotox- icities of QHEC and QHEC/DNA complexes were also evaluated in four cell lines which were relatively low com- pared with 25 kDa bPEI. All results indicated that these quaternized hydroxyethylcelluloses could be used as poten- tial gene delivery vectors.展开更多
Design and synthesis of a carbamate-linked cationic lipid DDCTMA (N-[1-(2,3-didodecylcarbamoyloxy)propyl]-N,N,N-trimethylammonium iodide)? as gene delivery carriers was described in this work. The transfection efficie...Design and synthesis of a carbamate-linked cationic lipid DDCTMA (N-[1-(2,3-didodecylcarbamoyloxy)propyl]-N,N,N-trimethylammonium iodide)? as gene delivery carriers was described in this work. The transfection efficiency of cationic liposome increased dramatically with the increase in the content of DOPE. In addition, the transfection efficiency of some of cationic lipoplexes was superior or parallel to that of two commercial transfection agents, Lipofectamine2000 and DOTAP. The carbamate-linked cationic lipid DDCTMA/DOPE may be a promising gene carrier that has high transfection efficiency as well as low cytotoxicity.展开更多
文摘Cationic polysaccharides have been receiving more attentions and used as nonviral gene delivery vectors. In this paper, quaternized hydroxyethylcellulose （QHEC） derivatives were studied as gene carriers for their efficient DNA binding abilities. All QHECs could form stable QHEC/DNA complexes and resist the degradation of DNase I. And the dynamic light scatter （DLS） results showed that all QHEC/DNA complexes could form compact particles. These QHEC/DNA complexes exhibited effective transfeetion abilities in comparison to the naked DNA. The cytotox- icities of QHEC and QHEC/DNA complexes were also evaluated in four cell lines which were relatively low com- pared with 25 kDa bPEI. All results indicated that these quaternized hydroxyethylcelluloses could be used as poten- tial gene delivery vectors.
文摘Design and synthesis of a carbamate-linked cationic lipid DDCTMA (N-[1-(2,3-didodecylcarbamoyloxy)propyl]-N,N,N-trimethylammonium iodide)? as gene delivery carriers was described in this work. The transfection efficiency of cationic liposome increased dramatically with the increase in the content of DOPE. In addition, the transfection efficiency of some of cationic lipoplexes was superior or parallel to that of two commercial transfection agents, Lipofectamine2000 and DOTAP. The carbamate-linked cationic lipid DDCTMA/DOPE may be a promising gene carrier that has high transfection efficiency as well as low cytotoxicity.
