Objective To observe the effect of artesunate (Art) on the proliferation of human esophageal carcinoma cell line Eca109 and the growth of transplantation tumors of nude mice, and explore the possible involvement of CD...Objective To observe the effect of artesunate (Art) on the proliferation of human esophageal carcinoma cell line Eca109 and the growth of transplantation tumors of nude mice, and explore the possible involvement of CDC25A expression in the cell cycle arrest induced by Art. Methods MTT method was employed to detect the proliferation of the Eca109 cells and normal human peripheral blood mononuclear cells (hPBMC) after Art treatment. Cell cycle of the tumor cells was assayed by flow cytometry. Inhibitory effects of Art on the transplanted tumor on nude mice were observed by mass weight, volume and morphological method. The expression of CDC25A in the Eca109 cells was examined by RT-PCR and Western blotting. Results Art significantly inhibited the proliferation of Eca109 with the IC50 of (68.80±0.76) μmol/L, while it had weaker effect on that of the hPBMC induced by Con A. At lower doses of Art, the number of Eca109 cells during G0/G1 was increased, and that at S phase was reduced dramatically. However, when the concentration was up to 100 μmol/L, most of cells were arrested at G2/M phase. The volume and weight of transplanted tumor receiving Art treatment were smaller and lower than those of control group, with the maximal inhibitory rate of 76.4%. Art dramatically inhibited the mRNA as well as protein expressions of CDC25A in the Eca109 cells. Conclusion Art can inhibit the proliferation of tumor cells and transplanted tumor without apparent side effect, possibly by the mechanism of modulating cell cycle through CDC25A down-regulation.展开更多
目的通过体外星形胶质细胞的划痕模型,观察槲皮素对星形胶质细胞增殖和其细胞周期的影响,并探究可能的信号通路,分析cdc25A的表达变化。方法经划痕处理过的原代培养的新生SD大鼠的星形胶质细胞用槲皮素处理后,将其放入37℃5%CO2孵箱中...目的通过体外星形胶质细胞的划痕模型,观察槲皮素对星形胶质细胞增殖和其细胞周期的影响,并探究可能的信号通路,分析cdc25A的表达变化。方法经划痕处理过的原代培养的新生SD大鼠的星形胶质细胞用槲皮素处理后,将其放入37℃5%CO2孵箱中进行培养。分别通过Click-iT Edu test,流式细胞术和Western Blotting检测槲皮素对其增殖、细胞周期的影响及细胞周期相关蛋白cdc25A表达的变化。结果与对照组相比,在第24h时就能明显观察到槲皮素抑制划痕的愈合;槲皮素处理24h后,其增殖细胞百分比从20.92%降低到2.74%,G1期星形胶质细胞百分比从82.04%增加到92.06%,其S和G2期有一个相应的减少;50μmol/L槲皮素处理24h以后细胞周期相关蛋白cdc25A的表达强度下调约50%,具有明显的统计学差异(F=40.579,P<0.01)。结论通过划痕模型的研究发现,槲皮素可以通过抑制星形胶质细胞的增殖来抑制划痕的愈合,并通过降低细胞周期相关蛋白cdc25A的表达将其阻断在G1期。展开更多
文摘Objective To observe the effect of artesunate (Art) on the proliferation of human esophageal carcinoma cell line Eca109 and the growth of transplantation tumors of nude mice, and explore the possible involvement of CDC25A expression in the cell cycle arrest induced by Art. Methods MTT method was employed to detect the proliferation of the Eca109 cells and normal human peripheral blood mononuclear cells (hPBMC) after Art treatment. Cell cycle of the tumor cells was assayed by flow cytometry. Inhibitory effects of Art on the transplanted tumor on nude mice were observed by mass weight, volume and morphological method. The expression of CDC25A in the Eca109 cells was examined by RT-PCR and Western blotting. Results Art significantly inhibited the proliferation of Eca109 with the IC50 of (68.80±0.76) μmol/L, while it had weaker effect on that of the hPBMC induced by Con A. At lower doses of Art, the number of Eca109 cells during G0/G1 was increased, and that at S phase was reduced dramatically. However, when the concentration was up to 100 μmol/L, most of cells were arrested at G2/M phase. The volume and weight of transplanted tumor receiving Art treatment were smaller and lower than those of control group, with the maximal inhibitory rate of 76.4%. Art dramatically inhibited the mRNA as well as protein expressions of CDC25A in the Eca109 cells. Conclusion Art can inhibit the proliferation of tumor cells and transplanted tumor without apparent side effect, possibly by the mechanism of modulating cell cycle through CDC25A down-regulation.
文摘目的通过体外星形胶质细胞的划痕模型,观察槲皮素对星形胶质细胞增殖和其细胞周期的影响,并探究可能的信号通路,分析cdc25A的表达变化。方法经划痕处理过的原代培养的新生SD大鼠的星形胶质细胞用槲皮素处理后,将其放入37℃5%CO2孵箱中进行培养。分别通过Click-iT Edu test,流式细胞术和Western Blotting检测槲皮素对其增殖、细胞周期的影响及细胞周期相关蛋白cdc25A表达的变化。结果与对照组相比,在第24h时就能明显观察到槲皮素抑制划痕的愈合;槲皮素处理24h后,其增殖细胞百分比从20.92%降低到2.74%,G1期星形胶质细胞百分比从82.04%增加到92.06%,其S和G2期有一个相应的减少;50μmol/L槲皮素处理24h以后细胞周期相关蛋白cdc25A的表达强度下调约50%,具有明显的统计学差异(F=40.579,P<0.01)。结论通过划痕模型的研究发现,槲皮素可以通过抑制星形胶质细胞的增殖来抑制划痕的愈合,并通过降低细胞周期相关蛋白cdc25A的表达将其阻断在G1期。
