Cyclin-dependent kinase 2-associated protein 1（CDK2AP1）, a cell growth inhibitory factor, is abnormally expressed in cancer cells, and might be implicated in the development of lung cancer. However, no studies on th...Cyclin-dependent kinase 2-associated protein 1（CDK2AP1）, a cell growth inhibitory factor, is abnormally expressed in cancer cells, and might be implicated in the development of lung cancer. However, no studies on the function of CDK2AP1 in human lung cancer have been yet reported. In this study, overexpressing lentiviral vectors containing full-length CDK2AP1 cDNA and CDK2AP1 shRNA（short hairpin RNA） were constructed. Our results show that infecting A549 cells with lentivirus containing CDK2AP1 shRNA or full-length CDK2AP1 cDNA results in significantly down- or up-regulated the expression of CDK2AP1, respectively, thereby providing reliable tools for studying the function of CDK2AP1 in pulmonary carcinogenesis. Our data of MTT assay and flowcytometric analysis demonstrate that CDK2AP1 plays an important role in the proliferation/growth and cell cycling of A549 cells in vitro, and further investigation into its underlying mechanism of pulmonary carcinogenesis is needed.展开更多
目的研究在口腔黏膜白斑和口腔鳞癌组织中Bcl-2和CDK2AP1的表达情况及临床意义。方法运用免疫组织化学和实时荧光定量PCR技术对组织中Bcl-2、CDK2AP1表达进行检测。结果在口腔正常黏膜组织、口腔黏膜白斑组织和口腔鳞癌组织中Bcl-2蛋白...目的研究在口腔黏膜白斑和口腔鳞癌组织中Bcl-2和CDK2AP1的表达情况及临床意义。方法运用免疫组织化学和实时荧光定量PCR技术对组织中Bcl-2、CDK2AP1表达进行检测。结果在口腔正常黏膜组织、口腔黏膜白斑组织和口腔鳞癌组织中Bcl-2蛋白细胞阳性率依次为(2.72±1.45)%、(19.29±8.18)%和(57.20±19.37)%;CDK2AP1蛋白细胞阳性率依次为(62.68±11.07)%、(25.68±8.17)%和(7.87±2.18)%。Bcl-2和CDK2AP1 m RNA表达水平与蛋白表达水平一致。各组之间差异有统计学意义(P<0.05)。结论随着口腔病变恶性程度增高,在口腔正常黏膜、口腔黏膜白斑和口腔鳞癌中Bcl-2表达水平上升,而CDK2AP1表达水平降低,这2个指标可能与口腔肿瘤的发生、发展及预后有关。展开更多
基金Supported by the Grants from the Project of Scientific Innovation and Creative for Jilin Provincial Oversea Scholars(No.2010273)Jilin Provincial Science and Technology Services(Nos.20050408, 20070720, 200805120, 20090732)+2 种基金Jilin Provincial Development and Reformation Committee(No.2009Y042J12314)Jilin Province Talent Development Foundation (No.JRJB2007-2)the Natural Science Foundation of China(Nos.30670301, 30870354)
文摘Cyclin-dependent kinase 2-associated protein 1（CDK2AP1）, a cell growth inhibitory factor, is abnormally expressed in cancer cells, and might be implicated in the development of lung cancer. However, no studies on the function of CDK2AP1 in human lung cancer have been yet reported. In this study, overexpressing lentiviral vectors containing full-length CDK2AP1 cDNA and CDK2AP1 shRNA（short hairpin RNA） were constructed. Our results show that infecting A549 cells with lentivirus containing CDK2AP1 shRNA or full-length CDK2AP1 cDNA results in significantly down- or up-regulated the expression of CDK2AP1, respectively, thereby providing reliable tools for studying the function of CDK2AP1 in pulmonary carcinogenesis. Our data of MTT assay and flowcytometric analysis demonstrate that CDK2AP1 plays an important role in the proliferation/growth and cell cycling of A549 cells in vitro, and further investigation into its underlying mechanism of pulmonary carcinogenesis is needed.
文摘目的研究在口腔黏膜白斑和口腔鳞癌组织中Bcl-2和CDK2AP1的表达情况及临床意义。方法运用免疫组织化学和实时荧光定量PCR技术对组织中Bcl-2、CDK2AP1表达进行检测。结果在口腔正常黏膜组织、口腔黏膜白斑组织和口腔鳞癌组织中Bcl-2蛋白细胞阳性率依次为(2.72±1.45)%、(19.29±8.18)%和(57.20±19.37)%;CDK2AP1蛋白细胞阳性率依次为(62.68±11.07)%、(25.68±8.17)%和(7.87±2.18)%。Bcl-2和CDK2AP1 m RNA表达水平与蛋白表达水平一致。各组之间差异有统计学意义(P<0.05)。结论随着口腔病变恶性程度增高,在口腔正常黏膜、口腔黏膜白斑和口腔鳞癌中Bcl-2表达水平上升,而CDK2AP1表达水平降低,这2个指标可能与口腔肿瘤的发生、发展及预后有关。
