期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Stem cell properties and neural differentiation of sheep amniotic epithelial cells
1
作者 Xuemin Zhu Xiumei Wang +7 位作者 Guifang Cao Fengjun Liu Yinfeng Yang Xiaonan Li Yuling Zhang Yan Mi Junping Liu Lingli Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2013年第13期1210-1219,共10页
This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that th... This study was designed to verify the stem cell properties of sheep amniotic epithelial cells and their capacity for neural differentiation. Immunofluorescence microscopy and reverse transcription-PCR revealed that the sheep amniotic epithelial cells were positive for the embryonic stem cell marker proteins SSEA-1, SSEA-3, SSEA-4, TRA-1-60 and TRA-1-81, and the totipotency-associated genes Oct-4, Sox-2 and Rex-1, but negative for Nanog. Amniotic epithelial cells expressed β-Ⅲ-tubulin, glial fibrillary acidic protein, nestin and microtubule-associated protein-2 at 28 days after induction with serum-free neurobasal-A medium containing B-27. Thus, sheep amniotic epithelial cells could differentiate into neurons expressing β-Ⅲ-tubulin and microtubule-associated protein-2, and glial-like cells expressing glial fibrillary acidic protein, under specific conditions. 展开更多
关键词 neural regeneration stem cells SHEEP amniotic epithelial cells isolation and culture stem cecharacteristics DIFFERENTIATION differentiation potential reverse transcription-PCR immunofluorescence microscopy grants-supported paper NEUROREGENERATION
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部